Development of highly productive medicinal plants utilizing tissue-specific gene expression systems

• Project/Area Number: 19590117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: University of Toyama
• Principal Investigator: KUROSAKI Fumiya University of Toyama, 大学院・医学薬学研究部(薬学), 准教授 (70143865)
• Co-Investigator(Kenkyū-buntansha): YAMADA Kyoji 富山大学, 大学院・医学薬学研究部(薬学), 教授 (70200714) ; LEE Jung-bum 富山大学, 大学院・医学薬学研究部(薬学), 助教 (40332655)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 薬用資源学 / 細胞内情報伝達系 / 遺伝子組み換え植物 / GTP結合タンパク質 / Rac / Rop GTPase / 遺伝子クローニング / 発現解析 / ゲノム解析 / 細胞内情報伝達 / 植物二次代謝 / αサブユニット / 二次代謝

Research Abstract

高等植物の二次代謝能の活性化にかかわる細胞内情報伝達系構成因子のうち、GTP結合タンパク質をコードする遺伝子を単離し器官特異的に過剰発現させて、物質生産への応用の可能性を検討した。ヘテロ三量体Gタンパクのαサブユニット遺伝子は刺激に対して殆んど応答しなかったが、単量体GTPase遺伝子の一部は転写レベルが上昇し、これを根組織で発現させた組み換え植物の分泌活性が顕著に上昇することを見出した。

Research Products

• [Journal Article] Cloning and characterization of a gene encoding Rac/Ro like monomeric guanosine 5'-triphosphate-binding protein from Scoparia dulcis (2009)
  • Author(s): Mitamura, T, Shite, M., Yamamura, Y., Kurosaki, F.
  • Journal Title: Biol. Pharm. Bull 32( In press)
  • Peer Reviewed
• [Journal Article] Cloning and characterization of Sdga gene encoding α-subunit of heterotrimeric GTP-binding protein complex in Scoparia dulcis (2008)
  • Author(s): Shite, M, Yamamura, Y., Hayashi, T, Kurosaki, F
  • Journal Title: Biol. Pharm. Bull 31 Pages: 2150-2153
  • Peer Reviewed
• [Journal Article] Two RNA editing sites with cis-acting elements of moderate sequence identity are recognized by an identical site-recognition protein in tobacco chloroplasts (2008)
  • Author(s): Kobayashi, Y., Matsuo, M., Sakamoto, K., Wakasugi, T., Yamada, K, Obokata J
  • Journal Title: Nucleic Acids Res 31 Pages: 752-754
  • Peer Reviewed
• [Journal Article] Effects of topical application of tacrolimus on acute itch-associated responses in mice (2008)
  • Author(s): Nakano T, Andoh T, Tayama M., Kosaka M., Lee J.-B, and Kuraishi Y
  • Journal Title: Biol. Pharm. Bull 31 Pages: 752-754
  • Peer Reviewed
• [Journal Article] Different dorsal horn neurons responding to histamine and allergic itchstimuli (2008)
  • Author(s): Nakano T, Andoh T, Lee J.-B, Kuraishi Y.
  • Journal Title: Neuroreport 19 Pages: 723-726
  • Peer Reviewed
• [Journal Article] Activation of proteinase-activated receptors induces itch associated response through histamine-dependent and independent pathways in mice (2008)
  • Author(s): Tsujii K, Andoh T, Lee J.-B, Kuraishi Y.
  • Journal Title: J. Pharmacol. Sci 108 Pages: 385-388
  • Peer Reviewed
• [Journal Article] Enhanced secretory activity of Atropa belladonna hairy root culture over-expressing ADP-ribosylation factor gene (2008)
  • Author(s): Asakura, Y, Seki, H, Muranaka, T, Yamamura, Y, Kurosaki, F.
  • Journal Title: Biol. Pharm. Bull 31 Pages: 1465-1468
  • NAID: 110006792122
  • Peer Reviewed
• [Journal Article] Structure and antiviral activity of an acidic polysaccharide from an edible blue-green alga (2008)
  • Author(s): Kanekiyo K, Hayashi K, Lee J.-B, Takenaka H, and Hayashi T
  • Journal Title: Nostoc flagelliforme. Yakugaku Zasshi 128 Pages: 725-731
  • Peer Reviewed
• [Journal Article] Cloning and characterization of Sdga gene encoding α-subunit of heterotrimeric GTP-binding protein complex in Scoparia dulcis. (2008)
  • Author(s): Shite, M., et al.
  • Journal Title: Biol. Pharm. Bull. 31 Pages: 2150-21534
  • Peer Reviewed
• [Journal Article] Enhanced secretory activity of Atropa belladonna hairy root culture over-expressing ADP-ribosylation factor gene. (2008)
  • Author(s): Asakura Y., et al.
  • Journal Title: Biol. Pharm. Bull. 31 Pages: 1465-1468
  • Peer Reviewed
• [Journal Article] Effects of topical application of tacrolimus on acute itch-associated responses in mice. (2008)
  • Author(s): Nakano T., et al.
  • Journal Title: Biol. Pharm. Bull. 31 Pages: 752-754
  • Peer Reviewed
• [Journal Article] Two RNA editing sites with cis-acting elements of moderate sequence identity are recognized by an identical site-recognition protein in tobacco chloroplasts. (2008)
  • Author(s): Kobayashi, Y., et al.
  • Journal Title: Nucleic Acids Res. 36 Pages: 311-318
  • Peer Reviewed
• [Journal Article] Different dorsal horn neurons responding to histamine and allergic itch stimuli. (2008)
  • Author(s): Nakano T., et al.
  • Journal Title: Neuroreport 79 Pages: 723-726
  • Peer Reviewed
• [Journal Article] Activation of proteinase-activated receptors induces itch-associated response through histamine-dependent and -independent pathways in mice. (2008)
  • Author(s): Tsujii K., et al.
  • Journal Title: J. Pharmacol., Sci. 108 Pages: 385-388
  • Peer Reviewed
• [Journal Article] Structure and antiviral activity of an acidic polysaccharide from an edible blue-green alga, Nostoc flagelliforme. (2008)
  • Author(s): Kanekiyo K., et al.
  • Journal Title: Yakugaku Zasshi 128 Pages: 725-731
  • Peer Reviewed
• [Journal Article] Cloning and expression of Dcga gene encoding α-subunit of GTP binding protein in carrot seedlings (2007)
  • Author(s): Asakura, Y., Kurosaki, F
  • Journal Title: 30 Pages: 1800-1804
  • NAID: 110006380273
  • Peer Reviewed
• [Journal Article] Cloning and expression of cDNAs encoding ADP-ribosylation factor in carrot seedling (2007)
  • Author(s): Asakura, Y, Ishigaki, E, Sugiyama, R., Kurosaki, F.
  • Journal Title: Plant Science 172 Pages: 189-195
  • Peer Reviewed
• [Journal Article] Cloning and expression of calmodulin gene inScoparia dulcis. (2007)
  • Author(s): Saitoh, D, Asakura, Y, Kasidimoko, N, K, Shite, M, Sugiyama, R,, Lee, J.-B., Hayashi, T, Kurosaki F.
  • Journal Title: Biol. Pharm. Bull. 30 Pages: 1161-1163
  • Peer Reviewed
• [Journal Article] Effect of partial desulfation and oversulfation of sodium spirulan on the potency of anti-herpetic activities (2007)
  • Author(s): Lee J.-B., Hou X., Hayashi K., Hayashi T.
  • Journal Title: Carbohydr. Polym 69 Pages: 651-658
  • Peer Reviewed
• [Journal Article] Cordyceps militaris grown on germinated soybeans (2007)
  • Author(s): Ohta Y., Lee J.-B., Hayashi K., Fujita A., Park D.-K., Hayashi T.
  • Journal Title: J. Agric. Food Chem 55 Pages: 10194-10199
  • Peer Reviewed
• [Journal Article] Anti-herpes virus target of an acidic polysaccharide, nostoflan, from an edible alga (2007)
  • Author(s): Kanekiyo K., Hayashi K., Takenaka H., Lee J.-B., Hayashi T.
  • Journal Title: Nostoc fragelliforme. Biol. Pharm. Bull 30 Pages: 1573-1575
  • Peer Reviewed
• [Journal Article] Cloning and expression of cDNAs encoding ADP-ribosylation factor in carrot seedling (2007)
  • Author(s): Asakura, et. al.
  • Journal Title: Plant Science 172 Pages: 189-195
  • Peer Reviewed
• [Journal Article] Cloning and expression of calmodulin gene in Scoparia dulcis (2007)
  • Author(s): Saitoh, et. al.
  • Journal Title: Biol.Pharm.Bull 30 Pages: 1161-1163
  • Peer Reviewed
• [Journal Article] Effect of partial desulfation and oversu1fation of sodium spirulan on the potency of anti-herpetic activities (2007)
  • Author(s): LeeJ.B., et. al.
  • Journal Title: Carbohydr.Polym 69 Pages: 651-658
  • Peer Reviewed
• [Presentation] メチルジャスモン酸で誘導されるScoparia dulcisのジテルペン生合成活性化に関わる情報伝達関連遺伝子群のクローニング (2008)
  • Author(s): 志手 真人, 他
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-03-26
• [Presentation] 海藻由来硫酸化多糖の生物活性 (2008)
  • Author(s): 李貞範, 林京子, 林利光
  • Organizer: 第17回天然薬物の開発と応用シンポジウム
  • Place of Presentation: 福岡
• [Presentation] ツルムラサキ(Basella rubra)由来抗ウイルス活性多糖に関する研究 (2008)
  • Author(s): 水越悠輔, 李貞範, 林京子, 林利光
  • Organizer: 日本生薬学会第55回年会
  • Place of Presentation: 長崎
• [Presentation] Methyl jasmonate influences secondary metabolism and protein expression in Scoparia dulcis (2008)
  • Author(s): 3Lee J.-B., Suzuki S., Nkembo KM., Kayagi K., and Hayashi T.
  • Organizer: 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
  • Place of Presentation: Athens, Greece
• [Presentation] Usefulness of algal polysaccharides for viral infectious diseases (2008)
  • Author(s): Ohta Y., Lee J.-B., Hayashi K., Takeshita A., and Hayashi T.
  • Organizer: 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
  • Place of Presentation: Athens, Greece
• [Presentation] Anti-influenza A virus activity of an acidic polysaccharide from a blue-green alga Nostoc flagelliforme (2008)
  • Author(s): Hayashi K., Kanekiyo K., Ohta Y., Lee J.-B., Takenaka H., and Hayashi T
  • Organizer: 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
  • Place of Presentation: Athens, Greece
• [Presentation] Signal transduction mechanisms involved in induced biosynthesis of defense-related compound in carrot (2008)
  • Author(s): Shite M, Yamamura Y, Kurosaki F
  • Organizer: 5th International Conference on Plant Metabolomics
  • Place of Presentation: Yokohama
• [Presentation] Real-time analysis of cell wall degrading enzymes expression in plant-pathogenic fungus Fusarium verticillioides upon infection (2008)
  • Author(s): 7Yamamura Y, Kurosaki F
  • Organizer: 5th International Conference on Plant Metabolomics
  • Place of Presentation: Yokohama
• [Presentation] 緑藻ヒトエグサMonostroma nitidum由来硫酸化多糖の構造と生物活性 (2008)
  • Author(s): 李貞範, 小泉誠志, 林京子, 田中茂男, 林利光
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] 空心蓮子草Alternanthera philoxeroides由来ウイルス活性物質の作用特性 (2008)
  • Author(s): Rattananthongkom Ariya, 李貞範, 林京子, 林利光
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] 褐藻イトヨレモクSargassum trichophyllum由来生物活性多糖の探索 (2008)
  • Author(s): 竹下亜澄, 李貞範, 林京子, 林利光
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] メチルジャスモン酸で誘導されるScoparia dulcisのジテルペン生合成活性化に関わる情報伝達関連遺伝子群のクローニング (2008)
  • Author(s): 志手真人, 山村良美, 林利光, 黒崎文也
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] Methyl jasmonate influences secondary metabolism and proteinexpression in Scoparia dulcis. (2008)
  • Author(s): Lee J. -B., et al.
  • Organizer: 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
  • Place of Presentation: Athens, Greece
• [Presentation] Anti-influenza A virus activity of an acidic polysaccharide from a blue-green alga Nostoc flagelliforme. (2008)
  • Author(s): Hayashi K, et al.
  • Organizer: 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
  • Place of Presentation: Athens, Greece
• [Presentation] Usefulness of algal polysaccharides for viral infectious diseases. (2008)
  • Author(s): Ohta Y., et al.
  • Organizer: 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
  • Place of Presentation: Athens, Greece
• [Presentation] Sinai transduction mechanisms involved in induced biosynthesis of defense-related compound in carrot (2008)
  • Author(s): Shite M., et al.
  • Organizer: 5th International Conference on Plant Metabolomics
  • Place of Presentation: Yokohama