Rapid monitoring of bacteria in dialysis fluids and reverse-osmosis water purification system by fluorescent vital staining method

• Project/Area Number: 19590118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Osaka University
• Principal Investigator: YAMAGUCHI Nobuyasu Osaka University, 大学院・薬学研究科, 准教授 (20252702)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 透析液 / RO水 / 細菌モニタリング / 蛍光活性染色法 / 増殖活性 / 群集構造解析 / マイクロコロニー / 変性剤濃度勾配ゲル電気泳動法(6) / 細菌 / モニタリング

Research Abstract

透析液中の微生物モニタリングは、衛生微生物学的に安全な透析液の供給のために重要である。そこで、これまでに開発・検討してきた迅速検出法を用いて、透析液およびその原料となるRO 水調製システムにおける細菌の動態解析を行った。その結果、透析液中の細菌数の迅速モニタリングには、蛍光活性染色法およびマイクロコロニー法が適していること、透析液調製過程における細菌群集構造の変化および優占種の推定は、変性剤濃度勾配ゲル電気泳動法により可能であることがわかった。これらの方法を用いることにより、透析液やその調製システムの微生物学的な管理を高精度に行えることがわかった

Research Products

• [Journal Article] Staphylococcus epidermidis forms floating micro-colonies in platelet concentrates at the early stage of contamination (2009)
  • Author(s): N. Yamaguchi, et al.
  • Journal Title: J. Health Sci. Volume: 55 Pages: 726-731
  • NAID: 130000127960
  • Peer Reviewed
• [Journal Article] Rapid detection of starved Escherichia coli with respiratory activity in potable water by signal-amplified in situ hybridization following formazan reduction. (2009)
  • Author(s): N. Yamaguchi, et al.
  • Journal Title: Microbes Environ. Volume: 24 Pages: 286-290
  • NAID: 10025921064
  • Peer Reviewed
• [Journal Article] 透析液製造ラインにおける細菌の動態 (2007)
  • Author(s): 馬場貴志, 山口進康, 那須正夫
  • Journal Title: 腎と透析別冊「HDF 療法' 07」 Pages: 186-188
• [Journal Article] Rapid monitoring of bacteria in dialysis fluids by fluorescent vital staining and microcolony methods Nephrology Dialysis Transplantation (2007)
  • Author(s): N. Yamaguchi, T. Baba, S. Nakagawa, A. Saito and M. Nasu
  • Journal Title: 22 Pages: 612-616
  • Peer Reviewed
• [Journal Article] 透析液製造ラインにおける細菌の動態 (2007)
  • Author(s): 馬場貴志, 他.
  • Journal Title: 腎と透析別冊「HDF療法'07」 Pages: 186-188
• [Journal Article] Rapid monitoring of bacteria in dialysis fluids by fluorescent vital staining and microcolony methods (2007)
  • Author(s): Nobuyasu Yamaguchi, et al
  • Journal Title: Nephrology Dialysis Transplantation 22 Pages: 612-616
  • Peer Reviewed
• [Journal Article] Bacterial population dynamics in a reverse-osmosis water purification system determined by fluorescent staining and PCR-denaturing gradient gel electrophoresis
  • Author(s): N. Yamaguchi, T. Baba, R. Matsumoto and M. Nasu
  • Journal Title: Microbes Environ (in press)
  • NAID: 10025920303
  • Peer Reviewed
• [Presentation] Features and effectiveness of rapid bacterial detection system for contaminated blood products (2008)
  • Author(s): N. Yamaguchi
  • Organizer: American Association of Blood Bank 2008 Annual Meeting
  • Place of Presentation: モントリオール
  • Year and Date: 2008-10-04
• [Presentation] Features and Effectiveness of Rapid Bacterial Detection System for Contaminated Blood Products (2008)
  • Author(s): N. Yamaguchi
  • Organizer: American Association of Blood Bank 2008 Annual Meeting
  • Place of Presentation: Montreal
  • Year and Date: 2008-10-04
• [Presentation] Rapid enumeration of bacteria in contaminated platelet concentrates using a newly developed bio-imaging system following fluorescent vital staining (2008)
  • Author(s): N. Yamaguchi
  • Organizer: International Congress of International Society of Blood Transfusion
  • Place of Presentation: マカオ
  • Year and Date: 2008-06-09
• [Presentation] Rapid and sensitive detection of bacterial cells in contaminated platelet concentrates using a newly developed bio-imaging system (2008)
  • Author(s): N. Yamaguchi
  • Organizer: American Society for Microbiology 2008 General Meeting
  • Place of Presentation: ボストン
  • Year and Date: 2008-06-03
• [Presentation] 蛍光活性染色法による血小板製剤の迅速・高精度な細菌モニタリング (2008)
  • Author(s): 山口進康
  • Organizer: 第56回日本輸血・細胞治療学会総会
  • Place of Presentation: 福岡(招待講演)
  • Year and Date: 2008-04-27
• [Presentation] 蛍光活性染色法による血小板製剤の迅速・高精度な細菌モニタリング (2008)
  • Author(s): 山口進康
  • Organizer: 第56回日本輸血・細胞治療学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-04-27
• [Presentation] 血小板製剤中の細菌の蛍光活性染色法による迅速・高精度モニタリング (2008)
  • Author(s): 山口進康
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-26
• [Presentation] 透析液製造工程における細菌の動態解析 (2007)
  • Author(s): 山口進康
  • Organizer: 日本薬学会第127 年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-28