Identification and Functional Characterization of Pyrilamine Transporter, an Organic Cation Transporter

• Project/Area Number: 19590161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Teikyo University
• Principal Investigator: DEGUCHI Yoshlharu Teikyo University, 薬学部, 教授 (40254255)
• Co-Investigator(Kenkyū-buntansha): 黄倉 崇 帝京大学, 薬学部, 講師 (80326123)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 薬物動態学 / 血液脳関門 / 有機カチオントランスポーター / 不死化細胞株 / ピリラミン / PAMT / トランスポーター / 有機カチオン性物質 / モノアミン / PMAT / TR-BBB13 / siRNA / 有機カチオン性薬物 / OCTN2

Research Abstract

本研究課題は、血液脳関門(BBB)における有機カチオン輸送系ピリラミントランスポーターの分子実体の解明と輸送特性を明らかにすることを目的とした。本研究において、研究代表者は分子実体と輸送機能が異なる2つの有機カチオン性トランスポーターがBBBで機能していることを明らかにした。

Research Products

• [Journal Article] Involvement of the pyrilamine transporter, a putative organic cation transporter, in blood-brain barrier transport of oxycodone (2008)
  • Author(s): Okura T, Hattori A, Takano Y, Sato T, Hammarlund-Udenaes M, Terasaki T, Deguchi Y.
  • Journal Title: Drug Metab. Dispos 36 Pages: 2005-2005
• [Journal Article] Involvement of the pyrilamine transporter, a putative organic cationtransporter, in blood-brain barrier transport of oxycodone. (2008)
  • Author(s): Okura T, Deguchi Y, et al.
  • Journal Title: Drug Metab. Dispos. 36 Pages: 2005-2013
  • Peer Reviewed
• [Journal Article] Blood-brain barrier transport of pramipexole, a dopamine D2 agonist (2007)
  • Author(s): Okura T. Ito R, Ishiguro N, Tamai I, Deguchi Y.
  • Journal Title: Life Sci. 80 Pages: 1564-1564
• [Journal Article] Comparative evaluation of antinociceptive potency of morphine and morphine-6ss-glucuronide by simultaneous measurements of spinal concentration (2007)
  • Author(s): Okura T, Komiyama N, Morita Y, Deguchi Y, Yamada S.
  • Journal Title: Life Sci 80 Pages: 1319-1319
• [Journal Article] Comparative measurement of spinal CSF microdialysate concentrations and concomitant antinociception of morphine and morphine-6b-glucuronide in rats (2007)
  • Author(s): Okura T, Deguchi Y, et. al.
  • Journal Title: Life Science 80 Pages: 1319-1326
  • Peer Reviewed
• [Journal Article] Blood-brain barrier transport of pramipexole, a dopamine D2 agonist (2007)
  • Author(s): Okura T, Deguchi Y, et. al.
  • Journal Title: Life Science 80 Pages: 1564-1571
  • Peer Reviewed
• [Presentation] Drug-drug interactions between oxycodone and adjuvant analgesics at the blood-brain barrier (2009)
  • Author(s): Takashi Okura, Yusuke Nakazawa, Asami Hattori, Tetsuya Terasaki and Yoshiharu Deguchi
  • Organizer: 8th ISSX/22th JSSX Joint Meeting
  • Place of Presentation: abstract
• [Presentation] The pyrilamine transPorter functions as the multispecific organic cation transporter at the blood-brain barrier (2008)
  • Author(s): Keita Shimomura, Yoshiharu Deguchi et.al.
  • Organizer: 第23回日本薬物動態学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2008-10-31
• [Presentation] Plasma membrane monoamine trans- Porter(PMAT)mediates the efflux transport of org anic cations in the brain barrier system (2008)
  • Author(s): Takenori Sato, Yoshiharu Deguchi.et.al.
  • Organizer: 第23回日本薬物動態学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2008-10-31
• [Presentation] The pyrilamine transporter functions as the multispecific organic cation transporter at the blood-brain barrier (2008)
  • Author(s): Keita Shimomura, Yoshiharu Deguchi, et al
  • Organizer: 第23回日本薬物動態学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2008-10-31
• [Presentation] Plasma membrane monoamine transporter (PMAT) mediates the efflux transport of organic cations in the brain barrier system (2008)
  • Author(s): Takenori Sato, Yoshiharu Deguchi, et al
  • Organizer: 第23回日本薬物動態学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2008-10-31
• [Presentation] Takashi Okllra Yoshiharu Deguchi et.al. (2008)
  • Author(s): Takashi Okllra Yoshiharu Deguchi et.al.
  • Organizer: 11^<th> International Symposium on Signal Transduction in the Blood-Brain Barrier
  • Place of Presentation: オランダ・アムステルダム
  • Year and Date: 2008-09-20
• [Presentation] Expression and functional characterization of plasma membrane monoamine transporter(PMAT)in the rat BBB (2008)
  • Author(s): Yoshiharu Deguchi, et.al.
  • Organizer: 11^<th> International SymPosium on Signal Transduction in the Blood-Brain Barrier
  • Place of Presentation: オランダ・アムステルダム
  • Year and Date: 2008-09-20
• [Presentation] Blood-brain barrier transport and pharmacological effect of opioids (2008)
  • Author(s): Takashi Okura, Yoshiharu Deguchi, et al
  • Organizer: 11^<th> International Symposium on Signal Transduction in the Blood-Brain Barrier
  • Place of Presentation: オランダ・アムステルダム
  • Year and Date: 2008-09-20
• [Presentation] Expression and functional characterization of plasma membrane monoamine transporter (PMAT) in the rat blood-brain barrier (2008)
  • Author(s): Yoshiharu Deguchi, et al
  • Organizer: 11^<th> International Symposium on Signal Transduction in the Blood-Brain Barrier
  • Place of Presentation: オランダ・アムステルダム
  • Year and Date: 2008-09-20
• [Presentation] カチオントランスポーターPMATの脳関門における発現と輸送機能解析 (2008)
  • Author(s): 佐藤武則, 出口芳春, 他
  • Organizer: 日本薬剤学会第23年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-05-20
• [Presentation] 血液脳関門に高発現する新規有機カチオントランスポーター(PMAT)のクローニングと輸送機能解析 (2008)
  • Author(s): 高野祐輔、黄倉崇、出口芳春ら
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-26
• [Presentation] 脳関門におけるrat plasmame mebrane transporter(rPMAT)の発現と輸送機能 (2007)
  • Author(s): 高野祐輔、黄倉崇、出口芳春ら
  • Organizer: トランスポーター研究会第1回関東部会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-10
• [Presentation] Blood-brain barrier transport of oxycodone, an opioid receptor agonist (2007)
  • Author(s): Asami Hattori, Takashi Okura, Yusuke Nakazawa Margareta Hammarlund-Udenaes, Tetsuya Terasaki, Yoshiharu Deguchi
  • Organizer: The 5th International Symposium on Receptor Mechanisms, Signal Transduction and Drug Effects, Shizuoka p.95
  • Place of Presentation: abstract form
• [Presentation] 血液脳関門におけるカチオン性薬物トランスポーターの機能および輸送分子の解析 (2007)
  • Author(s): 出口芳春、黄倉崇、服部麻美、鈴木俊英、寺崎哲也
  • Organizer: 講演要旨集.p.405(2007)
  • Place of Presentation: 日本薬剤学会(大宮)
• [Presentation] 麻薬性鎮痛薬オキシコドンと鎮痛補助薬の相互作用解析 (2007)
  • Author(s): 黄倉崇、中澤佑介、服部麻美、寺崎哲也、出口芳春
  • Organizer: 講演要旨集, p.343(2007)
  • Place of Presentation: 日本薬剤学会(大宮)
• [Presentation] H+/organic cation antiporter is involved in the blood-brain barrier transport of cationic drugs (2007)
  • Author(s): Yoshiharu Deguchi, Takashi Okura, et.al.
  • Organizer: 4th World Conference on Drug Absorption, Transport and Delivery
  • Place of Presentation: Kanazawa
• [Presentation] Involvement of H+/organic cation antiporter in the blood-brain barrier transport of cationic drugs (2007)
  • Author(s): Yoshiharu Deguchi
  • Organizer: 10th International Symposium, Signal Transduction in the Blood-Brain Barrier
  • Place of Presentation: Postdam/Germany
• [Presentation] Expression and transport function of ratplasma membrane transporter(rPMAT)in the brain barrler system (2007)
  • Author(s): 黄倉崇、出口芳春ら
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 仙台
• [Presentation] 血液脳関門におけるカチオン性薬物トランスポーターの機能および輸送分子の解析 (2007)
  • Author(s): 出口 芳春, ら
  • Organizer: 日本薬剤学会
  • Place of Presentation: 大宮
• [Presentation] H+/organic cation antiporter is involved in the blood-brain barrier transport of cationic drugs (2007)
  • Author(s): Deguchi Y, et. al.
  • Organizer: 4th World Conference on Drug Absorption, Transport and Delivery
  • Place of Presentation: 金沢
• [Presentation] Involvement of H+/0rganic cation antiporter in the blood-brain barrier transport of cationic drugs (2007)
  • Author(s): Deguchi Y
  • Organizer: 10th International Symposium, Signal Transduction in the Blood-Brain Barrier
  • Place of Presentation: Postdam/Germany
• [Book] 血液脳関門トランスポーターと創薬、トランスポーター科学の最前線、(辻彰編) (2008)
  • Author(s): 出口芳春
  • Total Pages: 119
• [Book] 分布(辻彰編) (2007)
  • Author(s): 出口芳春
  • Total Pages: 98
  • Publisher: 南江堂
• [Book] トランスポーター科学最前線 (2007)
  • Author(s): 出口 芳春
  • Total Pages: 15
  • Publisher: 京都廣川書店