Research of signal transduction by cell adhesion molecules in spermatogenesis

• Project/Area Number: 19590172
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kanazawa University
• Principal Investigator: WAKAYAMA Tomohiko Kanazawa University, 医学系, 准教授 (70305100)
• Co-Investigator(Kenkyū-buntansha): ISEKI Shoichi 金沢大学, 医学系, 教授 (50167251)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 精巣 / 精子形成 / 細胞接着分子 / シグナル伝達 / 造精細胞 / セルトリ細胞 / ノックアウトマウス / 精子形成障害 / 免疫組織化学 / 抗体 / 電子顕微鏡 / マイクロアレイ

Research Abstract

細胞接着分子Cell adhesion molecule-1(Cadm1)のノックアウト(KO)マウスは精子形成障害を生じる。DNAマイクロアレイと定量的RT-PCR法によって、KOマウスにおいて発現が増加する細胞接着分子Myelin protein zero like-2(Mpzl2)を検出した。KOマウスでは、Mpzl2が代償性に機能することが示唆された。また、Cadm1と相互作用するPoliovirusreceptorのKOマウスでは精子形成障害は見られなかった。

Research Products

• [Journal Article] Role of the spermatogenic-Sertoli cell interaction through cell adhesion molecule-1 (CADM1) in spermatogenesis. (2009)
  • Author(s): Wakayama T, Iseki S.
  • Journal Title: Anat Sci Int. 84 Pages: 112-21
  • NAID: 10025550490
  • Peer Reviewed
• [Journal Article] Expression, localization and binding activity of the ezrin/radixin/moesin proteins in the mouse testis. (2009)
  • Author(s): Wakayama T, Nakata H, Iseki S., (他2名、1番目)
  • Journal Title: J Histochem Cytochem 57 Pages: 351-62
  • Peer Reviewed
• [Journal Article] Role of the spermatogenic-Sertoli cell interaction through cell adhesi on molecule-1(CADM1)in spermatogenesis. (2009)
  • Author(s): Wakayama T, Iseki S
  • Journal Title: Anat Sci Int 84 Pages: 112-121
  • Peer Reviewed
• [Journal Article] Expression, localization and binding activity of the ezrin/radixin/moesin proteins in the mouse testis. (2009)
  • Author(s): Wakayama T, Nakata H, Kurobo M, Sai Y, Iseki S
  • Journal Title: J Histochem Cytochem 57 Pages: 351-362
  • Peer Reviewed
• [Journal Article] 精子形成に必須の細胞接着分子CADM1/IGSF4A/SgIGSF. (2008)
  • Author(s): 若山友彦、井関尚一
  • Journal Title: 日本生殖免疫学会雑誌 23 Pages: 1-10
  • NAID: 130004490606
  • Peer Reviewed
• [Journal Article] 精子形成に必須の細胞接着分子CADM1/IGSF4A/SgIGSF (2008)
  • Author(s): 若山友彦, 井関尚一
  • Journal Title: 日本生殖免疫学会雑誌 23 Pages: 1-10
  • NAID: 130004490606
  • Peer Reviewed
• [Journal Article] 精子形成に関与する新規接着分子SgIGSF (2007)
  • Author(s): 若山友彦、井関尚一
  • Journal Title: 顕微鏡 42 Pages: 41-4
  • NAID: 10018912942
  • Peer Reviewed
• [Journal Article] Heterophilic Binding of the Adhesion Molecules Poliovirus Receptor and Immunoglobulin Superfamily 4A in the Interaction between Mouse Spermatogenic and Sertoli Cells. (2007)
  • Author(s): Wakayama T, Sai Y, Kato Y., (他7名、1番目)
  • Journal Title: Biol Reprod 76 Pages: 1081-1090
  • NAID: 120006380690
  • Peer Reviewed
• [Journal Article] Heterophilic Binding of the Adhesion Molecules Poliovirus Receptor and Immunoglobulin Superfamily 4A in the Interaction between.Mouse Spermatogenic and Sertoli Cells (2007)
  • Author(s): Wakayama, T. (他9名、1番目)
  • Journal Title: Biology of Reproduction 76巻 Pages: 1081-1090
  • Peer Reviewed
• [Presentation] American Expression, localization and function of the ezrin/radixin/moesin (ERM) protein family in the mouse testis. (2008)
  • Author(s): Wakayama T, Kurobo M, Iseki S.
  • Organizer: Society for Reproductive Immunology 28th Annual Meeting
  • Place of Presentation: Congress Plaza Hotel and Convention Center, Chicago (USA).
  • Year and Date: 2008-06-14
• [Presentation] 生殖細胞・セルトリ細胞間の細胞接着因子と精子形成調節機構.シンポジウム「精子形成過程に於ける細胞動態制御の分子解剖学」 (2008)
  • Author(s): 若山友彦、井関尚一
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分大学医学部(大分県)
  • Year and Date: 2008-03-29
• [Presentation] 細胞接着分子SglGSFIIGSF4A/CADM1とその細胞内アダプター分子Dal-1/EPB41L3の相互作用によるマウス精子形成調節機構 (2007)
  • Author(s): 若山 友彦, 他3名
  • Organizer: 日本アンドロロジー学会第26回学術大会
  • Place of Presentation: 千葉ディスニーアンバサダーホテル
  • Year and Date: 2007-07-06
• [Presentation] Role of SgIGSF/TSLC1/Necl-2 in the interaction between spermatogenic and Sertoli cells during mouse spermatogenesis. (2007)
  • Author(s): Wakayama T., Iseki S.
  • Organizer: XIX North American Testis Workshop "Chromosome Structure and Gene Expression"
  • Place of Presentation: Hyatt Regency, Tampa (USA).
  • Year and Date: 2007-04-19
• [Remarks] ホームページ等
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• [Remarks]
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• [Remarks]
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• [Remarks]
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