The novel mode of action of axon guidance molecules, semaphorins in tissue remodeling of mouse female genital organ

• Project/Area Number: 19590178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Meijo University (2008); Wakayama Medical University (2007)
• Principal Investigator: YUKAWA Kazunori Meijo University, 薬学部, 教授 (20301434)
• Co-Investigator(Kenkyū-buntansha): TAKEUCHI Noriko 名城大学, 薬学部, 助手 (80076728) ; 田中 哲二 和歌山県立医科大学, 医学部, 准教授 (80275255) ; 上山 敬司 和歌山県立医科大学, 医学部, 准教授 (50264875)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Tetsuji 和歌山県立医科大学, 医学部, 准教授 (80275255) ; UEYAMA Takashi 和歌山県立医科大学, 医学部, 准教授 (50264875)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 発生学 / 形態形成学 / 組織リモデリング / セマフォリン / アポトーシス

Research Abstract

マウスは5週齢の性ホルモン分泌急増に伴い皮膚近傍の膣末端部上皮細胞の細胞死(アポトーシス)が誘導され、膣腔が皮膚に貫通(膣開口)する組織リモデリング現象を経て生殖器発達が完成する。私共の解析から、セマフォリン分子群に属するセマフォリン4D(Sema4D)の遺伝子欠損マウスでは、膣開口が起こらず膣閉鎖を発症し、5週齢マウス膣末端部におけるアポトーシス細胞の顕著な減少が明らかになった。2007~2008年度科学研究費研究計画実施の結果、Sema4Dのマウス膣上皮細胞に対するアポトーシス誘導作用が証明され、Sema4Dによるアポトーシス誘導にはPlexin-B1受容体が関与することが判った。

Research Products

• [Journal Article] Sema4D/CD100 deficiency leads to superior performance in mouse motor behavior (2009)
  • Author(s): Yukawa K, Tanaka T, Takeuchi N, Iso H, Li L, Kohsaka A, Waki H, Miyajima M, Maeda M, Kikutani H, Kumanogoh A
  • Journal Title: Can J Neurol Sci 36 Pages: 349-355
  • Peer Reviewed
• [Journal Article] Irinotecan-induced ovarian follicular apoptosis is attenuated by deleting the kinase domain of death-associated protein kinase (2009)
  • Author(s): Li L, Tanaka T, Yukawa K, Akira S, Umesaki N
  • Journal Title: Int J Oncol 34 Pages: 905-914
  • Peer Reviewed
• [Journal Article] Irinotecan-induced ovarian follicular apoptosis is attenuated by deleting the kinase domain of death-associated protein kinase. (2009)
  • Author(s): Li L, Tanaka T, Yukawa K, Akira S, Umesaki N
  • Journal Title: International Journal of Oncolopgy 34 Pages: 905-914
  • Peer Reviewed
• [Journal Article] Microinjection of urocortin into the rat nucleus tractus solitarii decreases arterial blood pressure (2008)
  • Author(s): Yamazaki T, Waki H, Kohsaka A, Nakamura T, Cui H, Yukawa K, Maeda M
  • Journal Title: Auton Neurosci 142(1-2) Pages: 51-4
  • NAID: 130005449201
  • Peer Reviewed
• [Journal Article] Adrenomedullin 2 microinjection into the nucleus tractus solitarius elevates arterial pressure and heart rate in rats (2008)
  • Author(s): Cui H, Kohsaka A, Waki H, Gouraud S, Nakamura T, Yukawa K, Hatada A, Bhuiyan ME, Maeda M
  • Journal Title: Auton Neurosci 142(1-2) Pages: 45-50
  • Peer Reviewed
• [Journal Article] Experimental characterization of recurrent ovarian immature teratoma cells after optimal surgery (2008)
  • Author(s): Tanaka T, Toujima S, Utsunomiya T, Yukawa K, Umesaki N
  • Journal Title: Oncol Rep 20(1) Pages: 13-23
  • Peer Reviewed
• [Journal Article] Controlling death-associated protein kinase activity to protect kidney cell suicide in renal ischemia-reperfusion injury Review paper (2008)
  • Author(s): Yukawa K, Tanaka T, Takeuchi N, Waki H, Kohsaka A, Maeda M
  • Journal Title: Jpn J Clin Physiol 38(6) Pages: 243-250
• [Journal Article] The sympathoexcitatory pathway from the CVL to the RVL for controlling brain vessels. Review article (2008)
  • Author(s): Maeda M, Waki H, Kohsaka A, Yukawa K, Nakamura T
  • Journal Title: zu Chi Medical J 20(4) Pages: 243-7
• [Journal Article] Experimental characterization of recurrent ovarian immature teratoma cellsafter optimal surgery. (2008)
  • Author(s): Tanaka T, Toujima S, Utsunomiya T, Yukawa K, Umesaki N
  • Journal Title: Oncology Report 20 Pages: 13-23
  • Peer Reviewed
• [Journal Article] Adrenomedullin 2 microinjection into the nucleus tractus solitarius elevatesarterial pressure and heart rate in rats. (2008)
  • Author(s): Cui H, Yukawa K (9名中6番目)
  • Journal Title: Autonomic Neuroscience 142 Pages: 45-50
  • Peer Reviewed
• [Journal Article] Microinjection of urocortin into the rat nucleus tractus solitarii decreasesarterial blood pressure. (2008)
  • Author(s): Yamazaki T, Yukawa K (7名中6番目)
  • Journal Title: Autonomic Neuroscience 142 Pages: 51-54
  • Peer Reviewed
• [Journal Article] Radiation reduces carboplatin sensitivity and enhances nedaplatin sensitivity in cervical squamous cell carcinoma in vitro (2007)
  • Author(s): Tanaka T, Yukawa K, Umesaki N
  • Journal Title: uropean Journal of Gynaecological Oncology XXVIII n.5 Pages: 352-355
  • Peer Reviewed
• [Journal Article] Radiation reduces bleomycin sensitivity in cervical squamous cancer cells in vitro (2007)
  • Author(s): Tanaka T, Yukawa K, Umesaki N
  • Journal Title: European Journal of Gynaecological Oncology XXVIII n.4 Pages: 273-277
  • Peer Reviewed
• [Journal Article] Radiation reduces bleomycin sensitivity in cervical squamous cancer cells in vitro (2007)
  • Author(s): Tanaka T, Yukawa K, Umesaki N
  • Journal Title: European Journal of Gynaecological Oncology 28(4) Pages: 273-277
  • Peer Reviewed
• [Journal Article] Radiation reduces carboplatin sensitivity and enhances nedaplatin sensitivity in cervical squamous cell carcinoma in vitro (2007)
  • Author(s): Tanaka T, Yukawa K, Umesaki N
  • Journal Title: European Journal of Gynaecological Oncology 28(5) Pages: 352-355
  • Peer Reviewed
• [Presentation] 遺伝性白内障ラット(UPL)の白内障発症に伴うクリスタリン変化 (2008)
  • Author(s): 竹内典子、石神志浦、今村知紗、林理紀、本多祐美、長井紀章、伊藤吉將、亀井鑠、湯川和典
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Novel mode of action for axon guidance molecules, semaphorins in tissue remodeling of mouse genital organ (2008)
  • Author(s): Li L, Yukawa K, Bai T, Tanaka T, Waki H, Kohsaka A, Ueyama T, Kumanogoh A, Kikutani H, Maeda M
  • Organizer: 第85回日本生理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-25
• [Presentation] Novel mode of action for axon guidance molecules, semaphorins in tissue remodeling of mouse famale genital organ (2008)
  • Author(s): Li Li, Yukawa K, Bai T, Tanaka T, Waki H, Kohsaka A, Ueyama T, Kumanogoh A, Kikutani H, Maeda M
  • Organizer: 第85回日本生理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-25
• [Book] 『第26章視床と大脳皮質』『Essential Neuroscience』前田正信 (2008)
  • Author(s): 湯川和典、向阪彰、前田正信(訳)
  • Publisher: 監訳(丸善発行)