Cell lineage and functional analyses of the cranial sensory placodes in the vertebrate head

• Project/Area Number: 19590179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Jichi Medical University
• Principal Investigator: SATO Shigeru Jichi Medical University, 医学部, 講師 (70306108)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 感覚器プラコード / 比較ゲノム / 脊椎動物進化 / 転写制御 / エンハンサー / 器官形成 / 感覚器 / プラコード / 神経堤 / ニワトリ胚 / トランスジェニックマウス

Research Abstract

Six1 ホメオボックス遺伝子の発現解析により多様な感覚器プラコードの共通の原基であるpreplacodal region(PPR)の存在をマウスではじめて示した。次にニワトリとマウス胚の前側PPR でSix1 の発現を活性化する保存されたエンハンサーを同定した。Six1 前側PPR エンハンサーの活性化因子と抑制因子の有力な候補を同定し、Six1 発現領域(=PPR)が規定される分子基盤の一端を明らかにした。また、2 種類のSix1 プラコードエンハンサーの制御下でCre リコンビナーゼを発現するマウスの樹立に成功した。

Research Products

• [Journal Article] Multiple evolutionarily conserved enhancers control expression of Eya1. (2008)
  • Author(s): Ishihara T, Sato S, Ikeda K, Yajima H, Kawakami K.
  • Journal Title: Dev Dyn 237 Pages: 3142-3156
  • Peer Reviewed
• [Journal Article] Differential expression of Eya1 and Eya2 during chick early embryonic development. (2008)
  • Author(s): (2)Ishihara T, Ikeda K, Sato S, Yajima H, Kawakami K.
  • Journal Title: Gene Expr Patterns 8 Pages: 357-367
  • Peer Reviewed
• [Journal Article] Multiple evolutionarily conserved enhancers control expression of Eyal (2008)
  • Author(s): Ishihara, T.
  • Journal Title: Developmental Dynamics 237(11) Pages: 3142-3156
  • Peer Reviewed
• [Journal Article] Differential expression of Eya1 and Eya2 during chick early embryonic development (2008)
  • Author(s): Ishihara, T.
  • Journal Title: Gene Expression Patterns 8(5) Pages: 357-67
  • Peer Reviewed
• [Journal Article] Six1 is essential for early neurogenesis in the development of olfactory epithelium. (2007)
  • Author(s): Ikeda K, Ookawara S, Sato S, Ando Z, Kageyama R, Kawakami K.
  • Journal Title: Dev Biol 311 Pages: 53-68
  • Peer Reviewed
• [Journal Article] Six1 is essential for early neurogenesis in the development of olfactory epithelium (2007)
  • Author(s): Ikeda, K.
  • Journal Title: Developmental Biology 311(1) Pages: 53-68
  • Peer Reviewed
• [Presentation] Evolution of Six1 enhancers in vertebrates (2008)
  • Author(s): 佐藤 滋
  • Organizer: 第41回 日本発生生物学会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-29
• [Presentation] Characterization of Sx1 Enhancers. Implication for the Development and Evolution of PPR and Sensory Placodes. (2008)
  • Author(s): Sato S, Ikeda K, Hayashibara Y, Nakao K, Aizawa S, Ochi H, Ogino H, Kawakami K.
  • Organizer: The 16th CDB Meeting "Cis-sequence regulation and evolution"(要旨集122 ページ)
  • Place of Presentation: 理化学研究所 発生・再生科学総合研究センター(理研CDB)
• [Presentation] Evolution of Six1 enhancers - in vivo analysis using chick and mouse embryos. (2008)
  • Author(s): Sato S, Nakayama R, Ikeda K, Bunno T, Hayashibara Y, Aizawa S, Kawakami K.
  • Organizer: 日本発生生物学会第41 回大会(ポスター発表)
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Evolution of preplacodal region and sensory placodes (2008)
  • Author(s): 佐藤 滋
  • Organizer: 第16回 CDB meetingCis-sequence regulation and its evolution
  • Place of Presentation: 理研CDB
• [Presentation] Roles of Six genes in the proliferationand differentiation of muscle satellite cells (2007)
  • Author(s): 矢嶋 浩
  • Organizer: 第40回日本発生生物学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-30
• [Presentation] Placode enhancers of the mouse Six1 gene-in vivo analysis using chick and mouse embryos (2007)
  • Author(s): 佐藤 滋
  • Organizer: 第40回日本発生生物学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-28
• [Presentation] Placode enhancers of the mouse Six1 gene - in vivo analysis using chick and mouse embryos. (2007)
  • Author(s): Sato S, Nakayama R, Ikeda K, Hayashibara Y, Nakao K, Aizawa S, Kawakami K.
  • Organizer: 日本発生生物学会第40 回大会(ポスター発表)
  • Place of Presentation: 福岡国際会議場
• [Remarks]
  • URL: http://www.jichi.ac.jp/biol/home.html
• [Remarks]
  • URL: http://www.jichi.ac.jp/biol/home.html
• [Remarks]
  • URL: http://www.jichi.ac.jp/biol/home.html