The identification of amnion stem cells and the analysis of transitional organization according to process of differentiation
| Project/Area Number |
19590186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Toyama |
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Principal Investigator |
YOSHIDA Toshiko University of Toyama, 大学院・医学薬学研究部・再生医学講座, 准教授 (00171421)
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| Co-Investigator(Kenkyū-buntansha) |
NIKAIDO Toshio 富山大学, 大学院・医学薬学研究部, 教授 (50180568)
OKABE Motonori 富山大学, 大学院・医学薬学研究部, 助教 (60283066)
KATOU Ichiro 富山大学, 大学院・医学薬学研究部, 准
准教授 (50250741)
SAITHO Shigeru 富山大学, 大学院・医学薬学研究部, 教授 (30175351)
FUKUOKA Jyunnya 富山大学, 大学病院, 准教授 (00324575)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 羊膜細胞 / 幹細胞 / 細胞分化 / 組織形成 / 形態学的検討 / ヒト羊膜 / マウス羊膜 / 羊膜幹細胞 / 同定法 / 分化能 / 骨分化 / 神経分化 / 分化過程 |
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| Research Abstract |
ヒト羊膜上皮および羊膜間葉系細胞はsphere形成能が高く、単離細胞と比し、Oct3/4, Klf4,c-Myc,Sox2、NanogなどiPS細胞(誘導多能性幹細胞)作成に不可欠な転写因子の発現が高い。またマウス羊膜細胞では、SSEA-1(+)C-kit(-)でsphereを形成する細胞のみがinner cell massに導入され分化することが明確となった。Sphere形成と表面マーカーの発現を併せて評価することで、幹細胞の純化分画が可能となった。
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Report
(3 results)
Research Products
(95 results)
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[Journal Article] Mutation-,Aging-,and Gene Dosage-dependent Accumulation of Neuroserpin(G392E)in Endoplasmic Reticula and Lysosomes of Neurons in Transgenic Mice2008
Author(s)
Takasawa A, Kato I., Takasawa K, Ishii Y, Yoshida T, Shehata M, Kawaguchi H, Mohafez O, Sasahara M, and Hiraga K
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Journal Title
Journal of Biological Chemistry 283
Pages: 35606-35613
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[Journal Article] Zisch A, Strom SC.Isolation and Characterization of Cells from Human Term Placenta., Outcome of the First International Workshop on Placenta Derived Stem Cells2008
Author(s)
Parolini O, Alviano F, Bagnara GP, Bilic G, Buhring H-J, Evangelista M, Hennerbichler S, Liu B, Magatti M, Mao N, Miki T, Marongiu F, Nakajima H, Nikaido T, Portmann-Lanz C-B, Sankar V, Soncini M, Stadler G, Surbek D, Takahashi T, Redle H, Sakuragawa N, Wolbank S, Zeisberger S
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Journal Title
Stem Cells 26
Pages: 300-311
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[Journal Article] Mutation- aging-, and gene-dosage-dependent accumulation of FENIB-type meuroserpin (G392E) in ERs and lysosomes of neurons in transgenic mice.2008
Author(s)
Akira Takasawa, Ichiro Kato, Kumi Takasawa, Yoko Ishi, Toshiko Yoshida Mohammad H. Shehata, hiroshi Kawaguchi, Omar M. M., ohafez, Masakiyo Sasahara, Koichi Hiraga
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Journal Title
J Biol Chem 283(51)
Pages: 35606-13
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Peer Reviewed
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[Journal Article] Strom SC. Isolation and Characterization of Cells from Human Term Placenta.2008
Author(s)
1) Parolini O., Alviano F., Bagnara GP., Bilic G., Buhring H-J., Evangelista M., Hennerbichler S., Liu B., Magatti M., Mao N., Miki T., Marongiu F., Nakajima H., Nikaido T., Portmann-Lanz C-B., Sankar V., Soncini M., Stadler G., Surbek D., Takahashi T., Redle H., Sakuragawa N., Wolbank S., Zeisberger S., Zisch A
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Journal Title
Outcome of the First International Workshop on Placenta Derived Stem Cells. Stem Cells. 26
Pages: 300-311
Related Report
Peer Reviewed
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[Journal Article] Lysocellin,a metabolite of the novel drug `alopestatin`,induces G1 arrest and prevents cytotoxicity induced by etoposide2006
Author(s)
Takahara Y, Yogosawa S, Maruyama S, Watanabe N, Yokoyama H, Fukasawa K, Sukenaga Y, Kamiyama J, Izumi M, Wakada M, Zhang H, Yoshizawa K, Kawa S, Nikaido T, Sakai T
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Journal Title
Int J Oncol 28
Pages: 823-829
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[Presentation] マウス羊膜幹細胞の分化能2009
Author(s)
吉田淑子, Teng Zan, 周 凱旋, 岡部素典, 小池千加, 米田徳子, 野上真紀子, 樋口 収, 二階堂敏雄
Organizer
第8回日本再生医療学会総会
Place of Presentation
東京
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[Presentation] 褥創モデルラットを用いた不死化羊膜間葉細胞添加Collagen gelによる創傷治癒効果の検討2008
Author(s)
高橋雅法,吉田淑子,岡部素典,木村真梨,Teng Zan,戸田文香,米田徳子,京哲,清野透,柴原直利,二階堂敏雄
Organizer
第7回再生医療学会
Place of Presentation
名古屋
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