A role of fatty acid binding protein as a regulator of cellular functions

• Project/Area Number: 19590189
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Yamaguchi University
• Principal Investigator: OWADA Yuji Yamaguchi University, 大学院・医学系研究科, 教授 (20292211)
• Co-Investigator(Kenkyū-buntansha): TOKUDA Nobuko 山口大学, 大学院・医学系研究科, 講師 (70227578) ; ADACHI Yasuhiro 山口大学, 大学院・医学系研究科, 助教 (10346546)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 脂肪酸結合タンパク / 脳 / ノックアウトマウス / FABP / マウス / 肥満細胞 / 表皮細胞 / アストロサイト / 脂肪酸 / 細胞骨格

Research Abstract

FABP分子群の生体機能を明らかにすべく、細胞内シグナル伝達系の制御への関与とその機構、脂肪酸代謝物であるエイコサノイドの産生系への関与と活性の制御、等について焦点を当てながら検証を加えた。その結果、(1)表皮型FABP分子が、マウス肥満細胞において、LPS誘導TNFα産生経路にポジティブに関与していることが判明した。(2)表皮型FABP分子が、マウス表皮細胞においてリノール酸の代謝制御を介して、早期分化過程に関与していることが判明した。(3)脳型FABP分子が、マウスアストロサイトの脂肪酸代謝を介して、精神神経疾患の病態に関与していることが判明した。

Research Products

• [Journal Article] Cystainyl leukotrienes enhance the degradation of bone marrow-derived mast cells through the autocrine mechanism (2009)
  • Author(s): Kaneko, I., Suzuki, K., Matsuo, K., Kumagai, H., Owada, Y., Noguchi, N., Hishinuma, T. and Ono, M.
  • Journal Title: Tohoku J. Exp. Med 217 Pages: 185-191
• [Journal Article] Arachidonic acid drives postnatal neurogenesis and elicits a beneficial effect on prepulse inhibition a biological trait of psychiatric illnesses (2009)
  • Author(s): Maekawa, M., Takashima, N., Matsumata, M., Ikegami, S., Kontani, M., Hara, Y., Kawashima, H., Owada, Y., Kiso, Y., Yoshikawa, T., Inokuchi, K. and Osumi, N.
  • Journal Title: PLoS ONE 4 Pages: 5085-5085
• [Journal Article] Role of heart-type fatty acid binding protein in the brain function (2009)
  • Author(s): Motohashi, K., Yamamoto, Y., Shioda, N., Kondo, H., Owada, Y. and Fukunaga, K.
  • Journal Title: Yakugaku Zasshi 129(Japanese) Pages: 191-195
  • NAID: 110007046096
• [Journal Article] 高次脳機能における心臓型脂肪酸結合タンパク質の役割 (2009)
  • Author(s): 本橋慧樹, 他
  • Journal Title: 薬学雑誌 129 Pages: 191-195
  • Peer Reviewed
• [Journal Article] Loss of Hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration (2008)
  • Author(s): Tamai, K., Tanaka, N., Toyoshima, M, Yamamoto, N., Owada, Y., Kiyonari, H., Ueno, Y., Ono, M., Shimosegawa, T., Watanabe, M. and Sugamura, K.
  • Journal Title: Am J Pathol 173 Pages: 1806-1817
• [Journal Article] Mast cell hyperplasia in the skin of Dsg4 deficient hypertrichosis mice, which are long-living mutant of lupus-prone mice (2008)
  • Author(s): Zhang, M.I., Furukawa, H., Tokunaka, K.,Saiga, K., Date, F., Owada, Y., Nose, M. and Ono, M.
  • Journal Title: Immunogenetics 60 Pages: 599-607
• [Journal Article] Fatty acid binding protein regulates TNF-α production in mast cells (2008)
  • Author(s): Yamamoto, N., Kaneko, I., Motohashi, K., Sakagami, H., Adachi, Y., Tokuda, N., Sawada T., Furukawa, H., Ueyama, Y., Fukunaga, K., Ono, M., Kondo, H. and Owada, Y.
  • Journal Title: Prost Leuko Ess Fatty Acids 79 Pages: 21-26
• [Journal Article] Activation of PPARγ reverses a defect of surfactant synthesis in mice lacking two types of fatty acid binding protein (FABP) (2008)
  • Author(s): Schachtrup, C., Malcharek, S., Owada, Y., Binas, B., Kondo, H., Rustow, B., Galla, H.J. and Spener, F.
  • Journal Title: Biochem. Biophys. Acta 1781 Pages: 314-320
• [Journal Article] Fatty acid binding protein : localization and functional significance in the brain (2008)
  • Author(s): Owada, Y.
  • Journal Title: Tohoku J. Exp. Med 214 Pages: 213-220
• [Journal Article] Prostaglandin F_2_a regulates cytokine production of mast cell through the receptors for prostaglandin E (2008)
  • Author(s): Kaneko, I., Hishinuma, Y., Suzuki, K., Owada, Y., Kitanaka, N., Kondo, H., Goto, J. Furukawa, H. and Ono, M.
  • Journal Title: Biochem. Biophys. Res. Commun 367 Pages: 590-597
• [Journal Article] Prostaglandin F_<2a> regulates cytokine production of mast cell through the receptors for prostaglandin E. (2008)
  • Author(s): Kaneko I, et al.
  • Journal Title: Biochem. Biophvs. Res. Commun. 367 Pages: 590-597
  • Peer Reviewed
• [Journal Article] Fatty acid binding protein : localization and functional significance in the brain (2008)
  • Author(s): Owada Y
  • Journal Title: Tohoku J Exp Med 214 Pages: 213-220
  • Peer Reviewed
• [Journal Article] Activation of PPAR_γ reverses a defect of surfactant synthesis in mice lacking two types of fatty acid binding protein (FABP) (2008)
  • Author(s): Schachtrup C, et al.
  • Journal Title: Biochem Biophys Acta 1781 Pages: 314-320
  • Peer Reviewed
• [Journal Article] Fatty acid binding protein regulates TNF-α production in mastcells. (2008)
  • Author(s): Yaraamo to N, et al.
  • Journal Title: Prost Leuko Ess Fatty Acids 79 Pages: 21-26
  • Peer Reviewed
• [Journal Article] Mast cell hyperplasia in the skin of Dsg4 deficient hypertrichosis mice, which are long-living mutant of lupus-prone mice. (2008)
  • Author(s): Zang MI, et al.
  • Journal Title: Immnogenetics 60 Pages: 599-607
  • Peer Reviewed
• [Journal Article] Loss of Hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration (2008)
  • Author(s): Tamai K, et al.
  • Journal Title: Am J Pathol 173 Pages: 1806-1817
  • Peer Reviewed
• [Journal Article] Fatty acid binding protein:localization and functional significante in the brain. (2008)
  • Author(s): Owada, Y.
  • Journal Title: Tohoku J. Exp. Med. 214 Pages: 213-220
  • Peer Reviewed
• [Journal Article] Prostaglandin F_2_α regulates cytokine production of mast cell through the receptors for prostaglandin E. (2008)
  • Author(s): Kaneko, I
  • Journal Title: Biochem. Biophys. Res. Commun. 367 Pages: 590-597
  • Peer Reviewed
• [Journal Article] FABP7 maps to a quantitative trait locus for a schizopherenic endophenotype (2007)
  • Author(s): Watanabe, A., Toyota, T., Owada, Y., Hashimoto, K., Ishitsuka, Y., Ohba, H., Iwayama, Y., Nakaya, A., Hayashi, T., Maekawa, M., Ohnishi, T., Yamada, K., Kondo, H., Osumi, N. and Yoshikawa, T.
  • Journal Title: PLoS Biol 5 Pages: 297-297
• [Journal Article] Distinct developmental expression of two isoforms of Ca2+/ calmodulin-dependent protein kinase kinases and their involvement in hippocampal dendritic formation (2007)
  • Author(s): Kamata, A., Sakagami, H., Tokumitsu, H., Sanda, M., Owada, Y., Fukunaga, K. and Kondo, H.
  • Journal Title: Neurosci Lett 423 Pages: 143-148
• [Journal Article] Somatodendritic localization of EFA6A, a guanine nucleotid exchanging factor, and its possible interaction with α-actinin in denritic spine (2007)
  • Author(s): Sakagami, H., Homma, T., Sukegawa, J., Owada, Y., Yanagisawa, T. and Kondo, H.
  • Journal Title: Eur. J. Neurosci 25 Pages: 618-625
• [Journal Article] Retinoic acid induces in vitro angiogenesis via retinoic acid receptor : possible involvement of paracrine effects of endogenous vascular endothelial growth factor signaling (2007)
  • Author(s): Saito, A., Sugawara, A., Uruno, A., Kudo, M., Kagechika, H., Sato, Y., Owada, Y., Kondo, H., Imaizumi, M., Tsuchiya, S., Ito, S.
  • Journal Title: Endocrinology 148 Pages: 1412-1423
• [Journal Article] Enhanced expression of adipocyte-type fatty acid binding protein in the apoptotic lymphocyte (2007)
  • Author(s): Abdelwahab, SA., Owada, Y., Kitanaka, N., Adida, A., Sakagami, H., Watanabe, M., Spener, F. and Kondo, H.
  • Journal Title: Mol. Cell. Biochem 299 Pages: 99-107
• [Journal Article] Spatiotemporal expression of four isoforms of Ca^<2+>/calmodulin-dependent protein kinase I in brain and its possible roles in hippocampal dendritic formation (2007)
  • Author(s): Kamata, A., Sakagami, H., Tokumitsu, H., Owada, Y., Fukunaga, K. and Kondo, H.
  • Journal Title: Neurosci Res.57 Pages: 86-97
• [Journal Article] FABP7 maps to a quantitative trait locus for a schizopherenic endophenotype. (2007)
  • Author(s): Watanabe, A.
  • Journal Title: PLoS Biol. 5 Pages: 297-315
  • Peer Reviewed
• [Presentation] マウス胸腺ナース細胞における脂肪細胞型脂肪酸結合タンパク(A-FABP/FABP4)の局在 (2009)
  • Author(s): 安達泰弘
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-29
• [Presentation] 脳型脂肪酸結合タンパク(B-FABP/FABP7)は末梢免疫臓器のTRC(T zone reticular cell)に局在する (2009)
  • Author(s): 徳田信子
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-28
• [Presentation] 末梢免疫臓器における脳型脂肪酸結合タンパク(B-FABP/FABP7)の局在 (2008)
  • Author(s): 徳田信子、山本のり子、安達泰宏、澤田知夫、近藤尚武、大和田祐二
  • Organizer: 第113回日本解剖学会総会
  • Place of Presentation: 由布市
  • Year and Date: 2008-03-28
• [Presentation] 高次脳機能における心臓脂肪酸結合タンパク(H-FABP/FABP3)の役割 (2008)
  • Author(s): 本橋慧樹、山本のり子、安達泰宏、徳田信子、澤田知夫、福永浩司、近藤尚武、大和田祐二
  • Organizer: 第113回日本解剖学会総会
  • Place of Presentation: 由布市
  • Year and Date: 2008-03-28
• [Presentation] 高次脳機能における心臓型脂肪酸結合タンパク質(H-FABP/FABP3)の役割 (2008)
  • Author(s): 本橋 慧樹
  • Organizer: 第113回日本解剖学会総会
  • Place of Presentation: 大分大学医学部(由布市)
  • Year and Date: 2008-03-27