Analysis of neuronal feeding-regulating circuits involving ghrelin using the transgenic mice

• Project/Area Number: 19590196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Showa University
• Principal Investigator: KAGEYAMA Haruaki Showa University, 医学部, 助教 (00433839)
• Co-Investigator(Kenkyū-buntansha): ARATA Satoru 昭和大学, 遺伝子組換え実験室, 准教授 (20159502) ; SHIODA Seiji 昭和大学, 医学部, 教授 (80102375)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 摂食調節 / 遺伝子改変動物 / 緑色蛍光タンパク質 / 神経回路網 / 視床下部 / 分子・細胞神経科学 / 神経内分泌学 / 神経組織学 / トランスジェニックマウス / Cre-loxP / 摂食ペプチド / 分子神経生物学 / 分子生物学 / 神経伝達物質と受容体

Research Abstract

グレリンは成長ホルモンの分泌促進作用のみならず、摂食促進やエネルギー代謝調節に関わっている。中枢におけるグレリンネットワークを神経解剖学的に解明することは複雑な摂食調節機構を解明する上で重要である。本研究ではCre-loxP システムによってグレリン発現細胞特異的に緑色蛍光タンパク質を高発現する遺伝子改変動物の作製を目的とした。緑色蛍光タンパク質を検出することで、グレリン産生細胞の分布局在を同定した。

Research Products

• [Journal Article] Neuronal relationship between orexin-A- and neuropeptide Y-induced orexigenic actions in goldfish (2009)
  • Author(s): Kojima K, Kamijo M, Kageyama H, Uchiyama M, Shioda S, Matsuda K.
  • Journal Title: Neuropeptides 43(2) Pages: 63-71
  • Peer Reviewed
• [Journal Article] Transient increase in plasma oxidized LDL during the progression of atherosclerosis in apolipoprotein E knockout mice (2009)
  • Author(s): Kato R, Mori C, Kitazato K, Arata S, Obama T, Mori M, Takahashi K, Aiuchi T, Takano T, Itabe H.
  • Journal Title: Arterioscler Thromb Vasc Biol 29(1) Pages: 33-39
  • Peer Reviewed
• [Journal Article] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death (2009)
  • Author(s): Amano K, Fujii M, Arata S, Tojima T, Ogawa M, Morita N, Shimohata A, Furuichi T, Itohara S, Kamiguchi H, Korenberg J R, Arata A, Yamakawa K.
  • Journal Title: J Neurosci. 29(9) Pages: 2984-2996
  • Peer Reviewed
• [Journal Article] Neuronal relationship between orexin-A-and neuropeptide Y-induced orexigenic actions in goldfish. (2009)
  • Author(s): Kojima, Kenji
  • Journal Title: Neuropeptides 43 Pages: 63-71
  • Peer Reviewed
• [Journal Article] Visualization of ghrelin-producing neurons in the hypothalamic arcuate nucleus using ghrelin-EGFP transgenic mice (2008)
  • Author(s): Kageyama H, Kitamura Y, Hosono T, Kintaka Y, Seki M, Takenoya F, Hori Y, Nonaka N, Arata S, Shioda S.
  • Journal Title: Regul Pept 145(1-3) Pages: 116-121
  • Peer Reviewed
• [Journal Article] Neuronal interactions between neuropeptide W-and orexin-or melanin-concentrating hormone-containing neurons in the rat hypothalamus (2008)
  • Author(s): Takenoya F, Kitamura S, Kageyama H, Nonaka N, Seki M, Itabashi K, Date Y, Nakazato M, Shioda S.
  • Journal Title: Regul Pept 145(1-3) Pages: 159-164
  • Peer Reviewed
• [Journal Article] Neural networks of several novel neuropeptides involved in feeding regulation (2008)
  • Author(s): Shioda S, Takenoya F, Yagi M, Wang L, Hori Y, Kageyama H.
  • Journal Title: Nutrition 24(9) Pages: 848-853
  • Peer Reviewed
• [Journal Article] alpha-Melanocyte-stimulating hormone mediates melanin-concentrating hormone-induced anorexigenic action in goldfish (2008)
  • Author(s): Shimakura S, Miura T, Maruyama K, Nakamachi T, Uchiyama M, Kageyama H, Shioda S, Takahashi A, Matsuda K.
  • Journal Title: Horm Behav 53(2) Pages: 323-328
  • Peer Reviewed
• [Journal Article] Neuronal interaction between melanin-concentrating hormone-and alpha-melanocyte-stimulating hormone-containing neurons in the goldfish hypothalamus (2008)
  • Author(s): Shimakura S, Kojima K, Nakamachi T, Kageyama H, Uchiyama M, Shioda S, Takahashi A, Matsuda K.
  • Journal Title: Peptides 29(8) Pages: 1432-1440
  • Peer Reviewed
• [Journal Article] Neuropeptide W is expressed in the noradrenalin-containing cells in the rat adrenal medulla (2008)
  • Author(s): Seki M, Kageyama H, Takenoya F, Hirayama M, Kintaka Y, Inoue S, Matsuno R, Itabashi K, Date Y, Nakazato M, Shioda S.
  • Journal Title: Regul Pept 145(1-3) Pages: 147-152
  • Peer Reviewed
• [Journal Article] Delivery of galanin-like peptide to the brain: targeting with intranasal delivery and cyclodextrins (2008)
  • Author(s): Nonaka N, Farr S A, Kageyama H, Shioda S, Banks W A.
  • Journal Title: J Pharmacol Exp Ther 325(2) Pages: 513-519
  • Peer Reviewed
• [Journal Article] Inhibitory effect of chicken gonadotropin-releasing hormone II on food intake in the goldfish, Carassius auratus (2008)
  • Author(s): Matsuda K, Nakamura K, Shimakura S, Miura T, Kageyama H, Uchiyama M, Shioda S, Ando H.
  • Journal Title: Horm Behav 54(1) Pages: 83-89
  • Peer Reviewed
• [Journal Article] Morphological interaction between galanin-like peptide-and dopamine-containing neurons in the rat arcuate nucleus (2008)
  • Author(s): Kageyama H, Takenoya F, Hori Y, Yoshida T, Shioda S.
  • Journal Title: Regul Pept 145(1-3) Pages: 165-168
  • Peer Reviewed
• [Journal Article] Synaptic interaction between ghrelin- and ghrelin-containing neurons in the rat hypothalamus (2008)
  • Author(s): Hori Y, Kageyama H, Guan J L, Kohno D, Yada T, Takenoya F, Nonaka N, Kangawa K, Shioda S, Yoshida T.
  • Journal Title: Regul Pept 145(1-3) Pages: 122-127
  • Peer Reviewed
• [Journal Article] Synaptic relationships between proopiomelanocortin- and ghrelin-containing neurons in the rat arcuate nucleus (2008)
  • Author(s): Guan J L, Okuda H, Takenoya F, Kintaka Y, Yagi M, Wang L, Seki M, Hori Y, Kageyama H, Shioda S.
  • Journal Title: Regul Pept 145(1-3) Pages: 128-132
  • Peer Reviewed
• [Journal Article] Neuropeptide W in the rat pancreas : Potentiation of glucose-induced insulin release and Ca^<2+> influx through L-type Ca2+ channels in beta-cells and localization in islets (2008)
  • Author(s): Dezaki K, Kageyama H, Seki M, Shioda S, Yada T.
  • Journal Title: Regul Pept 145(1-3) Pages: 153-158
  • Peer Reviewed
• [Journal Article] Neural networks of several novel neuropeptides involved in feeding regulation (2008)
  • Author(s): Shioda, Seiji
  • Journal Title: Nutrition 24 Pages: 848-853
  • Peer Reviewed
• [Journal Article] Delivery of galanin-like peptide to the brain : targeting with intranasal delivery and cyclodextrins (2008)
  • Author(s): Nonaka, Naoko
  • Journal Title: J Pharmacol Exp Ther 325 Pages: 513-519
  • Peer Reviewed
• [Journal Article] Neuronal interaction between melanin-concentrating hormone-and alpha-melanocyte-stimulating hormone-containing neurons in the goldfish hypothalamus. (2008)
  • Author(s): Shimakura, Sei-Ichi
  • Journal Title: Peptides 29 Pages: 1432-1440
  • Peer Reviewed
• [Journal Article] Visualization of ghrelin-producing neurons in the hypothalamic arcuate nucleus using ghrelin-EGFP transgenic mice (2008)
  • Author(s): Kageyama, Haruaki
  • Journal Title: Regulatory Peptides 145(1-3) Pages: 116-121
  • Peer Reviewed
• [Journal Article] Peptidomic identification and biological validation of neuroendocrine regulatory Peptide-1 and-2. (2007)
  • Author(s): Yamaguchi H, Sasaki K, Satomi Y, Shimbara T, Kageyama H, Mondal M S, Toshinai K, Date Y, Gonzalez L J, Shioda S, Takao T, Nakazato M, Minamino N.
  • Journal Title: J Biol Chem 282(36) Pages: 26354-26360
  • Peer Reviewed
• [Journal Article] Impaired mast cell maturation and degranulation and attenuated allergic responses in Ndrg1-deficient mice (2007)
  • Author(s): Taketomi Y, Sunaga K, Tanaka S, Nakamura M, Arata S, Okuda T, Moon T C, Chang H W, Sugimoto Y, Kokame K, Miyata T, Murakami M, Kudo I.
  • Journal Title: J Immunol 178(11) Pages: 7042-7053
  • Peer Reviewed
• [Presentation] 脳内における摂食調節-その機構解明と臨床応用について- (2008)
  • Author(s): 塩田清二
  • Organizer: 第33回日本比較内分泌学会
  • Place of Presentation: 広島
  • Year and Date: 2008-12-05
• [Presentation] Morphorgical analysis of neuropeptide W (NPW)-containing neurons in feeding regulation (2008)
  • Author(s): Shioda S
  • Organizer: The 38th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-16
• [Presentation] The effects of galanin -like peptide (GALP) on autonomic nervous system (2008)
  • Author(s): Kageyama H
  • Organizer: The 38th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2008-11-15
• [Presentation] The effects of galanin-like peptide (GALP)on autonomic nervous system (2008)
  • Author(s): Kageyama, Haruaki
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-15
• [Presentation] Feeding regulation in brain : Neuropeptide W. Showa University International Symposium for Life Science (2008)
  • Author(s): Takenoya F
  • Organizer: 5th Annual Meeting New Fron tiers in Neuroscience : Transmitters/modulators in Health and Disease
  • Place of Presentation: Tokyo
  • Year and Date: 2008-09-02
• [Presentation] Feeding regulation in brain : Galanin-like peptide (GALP)Showa University International Symposium for Life Science 5th Annual Meeting New Frontiers in Neuroscience (2008)
  • Author(s): Haruaki Kageyama
  • Organizer: Transmitters/modulators in Health and Disease
  • Place of Presentation: Tokyo
  • Year and Date: 2008-09-02
• [Presentation] ニューロペプチドW含有ニューロンの免疫細胞化学的観察 (2008)
  • Author(s): 竹ノ谷文子
  • Organizer: 第35回日本神経内分泌学会・第23回日本下垂体研究会合同学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-08-29
• [Presentation] 視床下部-下垂体-副腎系におけるニューロペプチドWについての機能形態学的観察 (2008)
  • Author(s): 影山晴秋
  • Organizer: 第35回日本神経内分泌学会・第23回日本下垂体研究会合同学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-08-29
• [Presentation] Input of some feeding-regulating neurons revealed by a genetically encoded tracer (2008)
  • Author(s): Kageyama H, Takenoya F, Shioda S
  • Organizer: (Symposium). XIII International congress of histochemistry and cytochemistry
  • Place of Presentation: Gda?sk, Poland
  • Year and Date: 2008-08-24
• [Presentation] Neuron network of feeding -regulating neurons in the hypothalamus (2008)
  • Author(s): Takenoya F, Kageyama H, Shioda S
  • Organizer: (Symposium). XIII International congress of histochemistry and cytochemistry
  • Place of Presentation: Gdansk, Poland
  • Year and Date: 2008-08-24
• [Presentation] Input of some feeding-regulating neurons revealed by a genetically encoded tracer. (2008)
  • Author(s): Kageyama, Haruaki
  • Organizer: the 13th Congress of the International Federation of Societies for Histochemistry and Cytochemistry
  • Place of Presentation: Gdansk, Poland
  • Year and Date: 2008-08-24
• [Presentation] ガラニン様ペプチド(GALP)の経鼻的投与法による薬物動態 (2008)
  • Author(s): 影山晴秋
  • Organizer: 第5回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-10
• [Presentation] ラット脳内おけるニューロペプチドW(NPW)の分布と局在について (2008)
  • Author(s): 竹ノ谷文子
  • Organizer: 第5回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-10
• [Presentation] cre-loxPシステムによるGALP発現ニューロンのニューロンネットワークの解析 (2008)
  • Author(s): 影山晴秋
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2008-03-30
• [Presentation] ガラニン様ペプチド(GALP)による自律神経系への影響 (2008)
  • Author(s): 影山晴秋
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-30
• [Presentation] 摂食調節ニューロンの求心性神経回路網の形態機能解析(シンポジウム) (2008)
  • Author(s): 影山晴秋
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-28
• [Presentation] Cre-LoxPシステムによるCaMKII発現ニューロンのニューロンネットワークの解析 (2008)
  • Author(s): 影山晴秋
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-28
• [Presentation] ラット脳内におけるニューロペプチドW(NPW)の分布局在 (2008)
  • Author(s): 竹ノ谷文子
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-28
• [Presentation] ラット下垂体におけるニューロペプチドW/B受容体(GPR7)の分布・局在 (2008)
  • Author(s): 加藤佐知
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-28
• [Presentation] 新規GPCRリガンドによる食欲調節の機能形態学(シンポジウム) (2008)
  • Author(s): 塩田清二
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-25
• [Presentation] Neural network of some novel neuropeptides in feeding regulation (Symposium) (2008)
  • Author(s): Shioda S
  • Organizer: 9th International NPY Meeting
  • Place of Presentation: 沖縄
  • Year and Date: 2008-03-17
• [Presentation] GALP neuron network of feeding regulation in rat brain (2008)
  • Author(s): Takenoya F
  • Organizer: 9th International NPY Meeting
  • Place of Presentation: 沖縄
  • Year and Date: 2008-03-17
• [Presentation] Role of GALP on energy metabolism in the central nervous system (2008)
  • Author(s): Kageyama H
  • Organizer: 9th International NPY Meeting
  • Place of Presentation: 沖縄
  • Year and Date: 2008-03-17
• [Presentation] Relationship between orexigenic action of Ghrelin and of NPY in goldfish, Carassius auratus (2008)
  • Author(s): Miura T
  • Organizer: 9th International NPY Meeting
  • Place of Presentation: 沖縄
  • Year and Date: 2008-03-17
• [Presentation] Role of GALP on energy metabolism in the centraI nervous system (2008)
  • Author(s): Kageyama, Haruaki
  • Organizer: The 9th Neuropeptide Y lnternational Meeting in Okinawa
  • Place of Presentation: 沖縄県国頭郡恩納村名嘉真ヤーシ原2591-1
  • Year and Date: 2008-03-17
• [Presentation] ガラニン様ペプチドは内因性の発熱物質である (2008)
  • Author(s): 影山晴秋
  • Organizer: 第22回日本糖尿病・肥満動物学会年次学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-02-09
• [Presentation] グレリン-EGFP遺伝子改変動物を用いたグレリンニューロンの機能形態的解析 (2007)
  • Author(s): 影山晴秋
  • Organizer: 第54回昭和医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-10
• [Presentation] Visualization of ghrelin-producing neurons in the hypothalamic arcuate nucleus using the ghrelin-enhanced green fluorescent protein transgenic mice (2007)
  • Author(s): Kageyama H
  • Organizer: The 37th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: San Diego, CA,USA
  • Year and Date: 2007-11-05
• [Presentation] Visualization of ghrelin-producing neurons in the hypothalamic arcuatenucleus using the ghrelin-enhanced green fluorescent protein transgenic mice (2007)
  • Author(s): Kageyama, Haruaki
  • Organizer: Society for Neuroscience
  • Place of Presentation: アメリカ合衆国カリフォルニア州サンディエゴ
  • Year and Date: 2007-11-05
• [Presentation] 香りと摂食調節(シンポジウム) (2007)
  • Author(s): 塩田清二
  • Organizer: 第10回日本アロマセラピー学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2007-11-04
• [Presentation] 骨格筋でのグルコース利用を促進する視床下部オレキシン・ニューロンの調節作用とその生理的意義 (2007)
  • Author(s): 志内哲也
  • Organizer: 第28回日本肥満学会
  • Place of Presentation: 東京
  • Year and Date: 2007-10-19
• [Presentation] ラット脳内におけるNPWニューロンの形態学的解析 (2007)
  • Author(s): 塩田清二
  • Organizer: 第32回日本比較内分泌学会大会およびシンポジウム
  • Place of Presentation: 日光
  • Year and Date: 2007-10-12
• [Presentation] キンギョにおけるニューロメジンU(NMU)受容体cDNAの単離とNMU受容体mRNAの組織発現 (2007)
  • Author(s): 丸山圭介
  • Organizer: 第32回日本比較内分泌学会大会およびシンポジウム
  • Place of Presentation: 日光
  • Year and Date: 2007-10-12
• [Presentation] キンギョグレリンの単離・精製とその特徴付け (2007)
  • Author(s): 三浦徹
  • Organizer: 第32回日本比較内分泌学会大会およびシンポジウム
  • Place of Presentation: 日光
  • Year and Date: 2007-10-12
• [Presentation] ラット視床下部におけるNPWニューロンのニューロンネットワーク (2007)
  • Author(s): 塩田清二
  • Organizer: 第62回日本体力医学会大会
  • Place of Presentation: 秋田
  • Year and Date: 2007-09-16
• [Presentation] ガラニン様ペプチド(GALP)による末梢神経作用 (2007)
  • Author(s): 竹ノ谷文子
  • Organizer: 第62回日本体力医学会大会
  • Place of Presentation: 秋田
  • Year and Date: 2007-09-16
• [Presentation] キンギョの摂食制御機構におけるグレリンとオレキシンの相互作用 (2007)
  • Author(s): 三浦徹
  • Organizer: 第4回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12
• [Presentation] キンギョにおけるニューロメジンU(NMU)の摂食制御機能 (2007)
  • Author(s): 丸山圭介
  • Organizer: 第4回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12
• [Presentation] ラット脳内おけるニューロペプチドW(NPW)のニューロンネットワーク解析 (2007)
  • Author(s): 竹ノ谷文子
  • Organizer: 第4回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12
• [Presentation] 副腎髄質におけるニューロペプチドW(NPW)の発現 (2007)
  • Author(s): 関真由美
  • Organizer: 第4回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12
• [Presentation] ガラニン様ペプチド(GALP)による末梢神経への影響 (2007)
  • Author(s): 影山晴秋
  • Organizer: 第4回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12
• [Book] Feeding regulation in the brain : Role of galanin-like peptide (GALP) in Transmitters and modulators in health and disease, New Frontiersss in Neuroscience(Shioda S, Homma I, Kato N eds.) (2009)
  • Author(s): Kageyama H, Takenoya F, Shioda S
  • Publisher: Tokyo, Springer
• [Book] Feeding regulation in the brain : involvement of neuropeptide W in Transmitters and modulators in health and disease, New Frontiers in Neuroscience(Shioda S, Homma I, Kato N eds.) (2009)
  • Author(s): Takenoya F, Kageyama H, Date Y, Nakazato M, Shioda S
  • Publisher: Tokyo, Springer
• [Book] Increased behavioral activity with regular circadian rhythm in PACAP specific receptor (PAC1) transgenic mice, in Transmitters and modulators in health and disease, New Frontiers in Neuroscience(Shioda S, Homma I, Kato N eds) (2009)
  • Author(s): Arata S, Hosono T, Taketomi Y, Kageyama H, Nakamachi T, Shioda S
  • Publisher: Tokyo, Springer
• [Book] Feeding regulation in the brain : Role of galanin-like peptide (GALP) in Transmitters and modulators in health and disease, New Frontiers in Neuroscience (2009)
  • Author(s): Kageyama, Haruaki
  • Publisher: Springer
• [Book] Feeding regulation in the brain : involvement of neuropeptide W in Transmitters and modulators in health and disease, New Frontiers in Neuroscience (2009)
  • Author(s): Takenoya, Fumiko
  • Publisher: Springer