Molecular anatomy of traffic of TEX101, a GPI-anchored protein, via VAMP3

• Project/Area Number: 19590197
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Nippon Medical School
• Principal Investigator: TAKIZAWA Takizawa Nippon Medical School, 医学部, 助教 (40386157)
• Co-Investigator(Kenkyū-buntansha): TAKIZAWA Toshihiro 日本医科大学, 大学院・医学研究科, 教授 (90271220) ; ISHIKAWA Tomoko 日本医科大学, 医学部, 助教 (70212850) ; MISHIYA Takuya 日本医科大学, 大学院・医学研究科, 特別研究生 (00398877)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 組織細胞化学 / TEXIOI / VAMP3 / エレクトロポレーション / 精巣 / TEX101 / GPI蛋白質 / GFP / RNAi / v-SNARE

Research Abstract

生殖細胞に特異的に発現しているGIP-アンカー型蛋白質であるTEXIOI、およびその細胞内輸送関連分子VAMP3の機能解析のためのバイオイメージング技術の開発と、それを用いた分子解剖学的解析を行った。TEXIOI関連分子のGFP融合発現ベクター、およびTEXIOIをノックダウンするGFP融合shRNAベクターを作製した。マウス精巣を用いて、エレクトロポレーションによる遺伝子導入の詳細な検討を行い、生殖細胞への良好なベクター導入に成功し、新しいinvivo遺伝子導入技術の開発に結びついた。

Research Products

• [Journal Article] Correlative Fluorescence and Electron Microscopy in Tissues : Immunocytochemistry (2009)
  • Author(s): John M Robinson, Toshihiro Takizawa
  • Journal Title: J Microsc (in press)
  • Peer Reviewed
• [Journal Article] Correlative Fluorescence and Electron Microscopy in Tissues : Immun ocytochemistry (2009)
  • Author(s): John M. Robinson
  • Journal Title: J Microsc (in press)
  • Peer Reviewed
• [Journal Article] MicroRNA (miRNA) cloning analysis reveals sex differences in miRNA expression profiles between adult mouse testis and ovary (2008)
  • Author(s): Takuya Mishima, Takami Takizawa, Shan-Shun Luo, Osamu Ishibashi, Yutaka Kawahigashi, Yoshiaki Mizuguchi, Tomoko Ishikawa, Miki Mori, Tomohiro Kanda, Tadashi Goto, Toshihiro Takizawa
  • Journal Title: Reproduction 136 (6) Pages: 811-811
  • Peer Reviewed
• [Journal Article] Proteomics of the human placenta: promises and realities (2008)
  • Author(s): John M. Robinson
  • Journal Title: Placenta 29・2 Pages: 135-143
  • Peer Reviewed
• [Journal Article] Therapeutic use of short hairpin RNA in acute liver failure (2007)
  • Author(s): Yoshiaki Mizuguchi, Shigeki Yokomuro, Takuya Mishima, Yasuo Arima, Tetsuya Shimizu, Yutaka Kawahigashi, Toshihiro Takizawa, Takashi Tajiri
  • Journal Title: J Nippon Med Sch 74 Pages: 74-76
  • NAID: 130004146890
  • Peer Reviewed
• [Journal Article] Genomic organization and structure of the 5'flanking region of the TEX101 gene : alternative promoter usage and splicing generate transcript variants with distinct 5'untranslated region (2007)
  • Author(s): Hiroki Tsukamoto, Toshihiro Takizawa, Kenji Takamori, Hideoki Ogawa, Yoshihiko Araki
  • Journal Title: Mol Reprod Dev 74 Pages: 154-162
  • Peer Reviewed
• [Journal Article] Therapeutic use of short hairpin RNA in acute liver failure (2007)
  • Author(s): Yoshiaki Mizuguchi
  • Journal Title: J Nippon Med Sch 74・1 Pages: 74-76
  • Peer Reviewed
• [Journal Article] Genomic organization and structure of the 5'-flanking region of the TEX101 gene: alternative promoter usage and splicing generate transcript variants with distinct 5'-untranslated region (2007)
  • Author(s): Hiroki Tsukamoto
  • Journal Title: Mol Reprod Dev 74・2 Pages: 154-162
  • Peer Reviewed
• [Presentation] RNAiによるマウス精巣Tex101に関するin vivoノックダウン解析 : ベクターの作製とエレクトロポレーションによる精巣への導入条件検討 (2008)
  • Author(s): 森美貴、石橋宰、瀧澤敬美、荒木慶彦、佐藤陽子、菱川善隆、小路武彦
  • Organizer: 第49回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2008-10-05
• [Presentation] RNAiによるマウス精巣Tex101こ関するin vivo ノックダウン解析 : ベクターの作製とエレクトロポレーションによる精巣への導入条件検討 (2008)
  • Author(s): 瀧澤 俊広
  • Organizer: 第49回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2008-10-05