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The expression and function of double-stranded RNA-dependent protein kinase (PKR) in rat intestinal epithelial cells

Research Project

Project/Area Number 19590201
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

DOI Yoshiaki  University of Occupational and Environmental Health, Japan, 医学部, 教授 (30258602)

Co-Investigator(Kenkyū-buntansha) MORIMOTO Hiroyuki  産業医科大学, 医学部, 准教授 (30335806)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細胞分化 / 小腸上皮細胞 / アポトーシス / 二本鎖RNA依存性プロテインキナーゼ(PKR) / ハイドロコルチゾン / STAT-1 / PKR / ラット小腸上皮細胞 / PKR阻害薬 / IEC6細胞 / 免疫組織化学 / ヒドロコルチゾン / 合成二本鎖RNA / 細胞培養 / ラット壁細胞 / real time RT-PCR / ウェスタンブロッティング / 分化誘導 / 走査型電子顕微鏡
Research Abstract

Intestinal epithelial cells (IECs) are exposed to microbial and viral products, and serve as essential barriers to them. Since double-stranded RNA-dependent protein kinase (PKR) is involved in cellular antiviral response, cell differentiation and apoptosis, we tried to investigate the expression and roles of PKR in rat IECs. In this study, we showed that the expression of PKR in IECs of adult rats. We also demonstrated that PKR was expressed in cultured rat IECs. The level of PKR protein expression and the activity of alkaline phosphatase (ALP) increased in the cultured IECs in a time-dependent manner. Treatment with PKR inhibitor decreased ALP activity in the IECs. The addition of synthetic double-stranded RNA induced apoptosis in a dose-dependent manner in these cells. Treatment with hydrocortisone also provoked suppression of PKR expression in such cells. Thus, we concluded that PKR is expressed in IECs as potent barriers to antigens and is a possible modulator of the differentiation and apoptosis in rat IECs.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (40 results)

All 2010 2009 2008 2007

All Journal Article (16 results) (of which Peer Reviewed: 14 results) Presentation (23 results) Book (1 results)

  • [Journal Article] Functional expression of double-stranded RNA-dependent proein kinase in rat intestinal epithelial cells2010

    • Author(s)
      Sato, N. 、Morimoto, H. 、Baba, R. 、Nakamata, J. 、Doi, Y. 、Yamaguchi, K.
    • Journal Title

      J. Cell. Biochem. Vol.110

      Pages: 104-111

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expressions of the multidrug resistance-related proteins in the rat olfactory epithelium: A possible role in the phaseIIIxenobiotic metabolizing function2010

    • Author(s)
      Kudo, H. 、Doi, Y. 、Fujimoto, S.
    • Journal Title

      Neurosci. Lett. Vol.468、No.2

      Pages: 98-101

    • NAID

      120001702386

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expressions of the multidrug resistance-related proteins in the rat olfactory epithelium : A possible role in the phase III xenobiotic metabolizing function2010

    • Author(s)
      Kudo, H, et al.
    • Journal Title

      Neurosci. Lett. 468

      Pages: 98-101

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An integrin-activating peptide, PHSRN, ameliorates inhibitory effects of conventional peritoneal dialysis fluids on peritoneal wound healing2010

    • Author(s)
      Miyamoto, T, et al.
    • Journal Title

      Nephrol. Dial. Transplant. 25

      Pages: 1109-1119

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional expression of double-stranded RNA-dependent protein kinase in rat intestinal epithelial cells2010

    • Author(s)
      Sato, N, et al.
    • Journal Title

      J.Cell. Biochem. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)2009

    • Author(s)
      Kudo, H. 、Doi, Y. 、Ueda, H. 、Kaeriyama, M.
    • Journal Title

      Comp. Biochem. Physiol. A Mol. Inteqr. Physiol. Vol.154、No.1

      Pages: 142-150

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)2009

    • Author(s)
      Kudo, H, et al.
    • Journal Title

      Comp.Biochem. Physiol. A Mol.Inteqr.Physiol. 154

      Pages: 142-150

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunoreactivities for glutathione S-transferases and glutathione peroxidase in the lateral wall of pigmented and albino guinea pig cochlea2008

    • Author(s)
      Fujimura, T. 、Suzuki, H. 、Udaka, T. 、Shiomori, T. 、Mori, T. 、Inaba, T. 、Hiraki, N. 、Kayashima, K. 、Doi, Y.
    • Journal Title

      Med. Mol. Morphol. Vol.41、No.3

      Pages: 139-144

    • NAID

      10024381153

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antioxidant enzyme immunoreactivity in rat von Ebner gland after nickel treatment2008

    • Author(s)
      Shiraishi, M. 、Doi, Y. 、Kayashima, K. 、Fujimoto, S.
    • Journal Title

      Med. Mol. Morphol. Vol.41、No.1

      Pages: 44-52

    • NAID

      10024380732

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 乳飲期回腸吸収上皮におけるエンドサイトーシスの膜系と細胞骨格の関連2008

    • Author(s)
      馬場良子、佐藤永洋、土肥良秋、藤田守
    • Journal Title

      医学生物学電子顕微鏡技術学会誌 Vol.22、No.2

      Pages: 62-62

    • Related Report
      2009 Final Research Report
  • [Journal Article] Antioxidant enzyme immunoreactivity in rat von Ebner gland after nickel treatment.2008

    • Author(s)
      Shiraishi, M., et al.
    • Journal Title

      Medical Molecular Morphology 41

      Pages: 44-52

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms supporting a paracrine role of GABA in rat adrenal medullary cells.2008

    • Author(s)
      Matsuoka, H., et al.
    • Journal Title

      Journal of Physiology 586

      Pages: 4825-4842

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunoreactivities for glutathione S-transferases and glutathione peroxidase in the lateral wall of pigmented and albino guinea pig cochlea.2008

    • Author(s)
      Fujimura, T., et al.
    • Journal Title

      Medical Molecular Morphology 41

      Pages: 139-144

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential expression of protein phosphatase type 1 isotypes and nucleolin during cell cycle arrest2007

    • Author(s)
      Morimoto, H. 、Ozaki, A. 、Okamura, H. 、Yoshida, K. 、Amorim, B.R. 、Tanaka, H. 、Kitamura, S. 、Haneji, T.
    • Journal Title

      Cell Biolchem. Funct. Vol.25、No.4

      Pages: 369-375

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 怒ると怖い血管内皮細胞2007

    • Author(s)
      土肥良秋
    • Journal Title

      ミクロスコピア Vol.24、No.2

      Pages: 98-104

    • Related Report
      2009 Final Research Report
  • [Journal Article] Differential expression of protein phosphatase type 1 isotypes and nucleolinduring cell cycle arrest2007

    • Author(s)
      Morimoto, H, et. al.
    • Journal Title

      Cell Biochemistry and Function 25

      Pages: 369-395

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 新生仔ラット回腸吸収上皮細胞におけるエンドゾームの局在2010

    • Author(s)
      馬場良子, 他
    • Organizer
      第115回日本解剖学会
    • Place of Presentation
      盛岡
    • Year and Date
      2010-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 小腸上皮におけるPKRの発現と機能2010

    • Author(s)
      佐藤永洋, 他
    • Organizer
      第115回日本解剖学会
    • Place of Presentation
      盛岡
    • Year and Date
      2010-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] 新生仔ラット回腸吸収上皮細胞におけるエンドゾームの局在2010

    • Author(s)
      馬場良子, 他
    • Organizer
      第115回日本解剖学会
    • Place of Presentation
      岩手県民会館(岩手県)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 小腸上皮におけるPKRの発現と機能2010

    • Author(s)
      佐藤永洋, 他
    • Organizer
      第115回日本解剖学会
    • Place of Presentation
      岩手県民会館(岩手県)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細胞内タンパク質相互作用の検出2009

    • Author(s)
      森本景之, 他
    • Organizer
      第51回日本顕微鏡学会九州支部総会
    • Place of Presentation
      九州工業大学(福岡県)
    • Year and Date
      2009-12-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蛍光を用いたタンパク質相互作用と翻訳後修飾の解析2009

    • Author(s)
      森本景之, 他
    • Organizer
      第41回日本臨床分子形態学会
    • Place of Presentation
      神戸
    • Year and Date
      2009-09-04
    • Related Report
      2009 Final Research Report
  • [Presentation] 新生児(仔)回腸吸収上皮細胞のエンドサイトーシスに関与する膜系の発達2009

    • Author(s)
      馬場良子, 他
    • Organizer
      第41回日本臨床分子形態学会
    • Place of Presentation
      神戸
    • Year and Date
      2009-09-04
    • Related Report
      2009 Final Research Report
  • [Presentation] 蛍光を用いたタンパク質相互作用と翻訳後修飾の解析2009

    • Author(s)
      森本景之, 他
    • Organizer
      第41回日本臨床分子形態学会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新生児(仔)回腸吸収上皮細胞のエンドサイトーシスに関与する膜系の発達2009

    • Author(s)
      馬場良子, 他
    • Organizer
      第41回日本臨床分子形態学会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蛋白質脱リン酸化酵素阻害剤によるアポトーシス誘導における蛋白質相互作用2009

    • Author(s)
      森本景之, 他
    • Organizer
      第18回日本アポトーシス研究会
    • Place of Presentation
      長崎
    • Year and Date
      2009-08-02
    • Related Report
      2009 Final Research Report
  • [Presentation] 蛋白質脱リン酸化酵素阻害剤によるアポトーシス誘導における蛋白質相互作用2009

    • Author(s)
      森本景之, 他
    • Organizer
      第18回日本アポトーシス研究会
    • Place of Presentation
      長崎大学(長崎県)
    • Year and Date
      2009-08-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] タンパク質相互作用の細胞内可視化法を用いたPKRと基質タンパク質の解析2009

    • Author(s)
      森本景之, 他
    • Organizer
      第65回日本顕微鏡学会
    • Place of Presentation
      仙台
    • Year and Date
      2009-05-29
    • Related Report
      2009 Final Research Report
  • [Presentation] タンパク質相互作用の細胞内可視化法を用いたPKRと基質タンパク質の解析2009

    • Author(s)
      森本景之, 他
    • Organizer
      第65回日本顕微鏡学会
    • Place of Presentation
      仙台国際センター(宮城県)
    • Year and Date
      2009-05-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 胃粘膜における二本鎖RNA依存プロテインキナーゼ(PKR)の局在2009

    • Author(s)
      馬場良子, 他
    • Organizer
      第114回日本解剖学会
    • Place of Presentation
      岡山
    • Year and Date
      2009-03-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 胃粘膜における二本鎖RNA依存プロテインキナーゼ(PKR)の局在2009

    • Author(s)
      馬場良子, 他
    • Organizer
      第114回日本解剖学会
    • Place of Presentation
      岡 山
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] The effect of alcohol on the formation of a functional gut-like structure derived from mouse es cells2009

    • Author(s)
      Hirano, T, et al.
    • Organizer
      The 36th International Congress of Physiological Sciences
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 胎盤栄養から母乳栄養への移行期における小腸吸収上皮細胞の形態と機能の変化2008

    • Author(s)
      馬場良子, 他
    • Organizer
      第40回日本臨床分子形態学会
    • Place of Presentation
      福岡
    • Year and Date
      2008-10-03
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 急性腹膜障害モデルにおける高糖濃度腹膜透析液の創傷治癒遅延効果2008

    • Author(s)
      宮本哲, 他
    • Organizer
      第53回日本透析医学会
    • Place of Presentation
      神戸
    • Year and Date
      2008-06-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 急性腹膜障害モデルにおけるインテグリン活性化ペプチドの創傷治癒促進効果2008

    • Author(s)
      宮本哲, 他
    • Organizer
      第51回日本腎臓学会
    • Place of Presentation
      福岡
    • Year and Date
      2008-05-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] The PHSRN sewuqnce reduces inhibitory effects conventional peritoneal dialysis solutions on integrin-mediated wound healing of rat peritoneal membrane.2008

    • Author(s)
      Miyamoto, T., et al.
    • Organizer
      11th Asian Pacific Congress of Nephrology
    • Place of Presentation
      Kuala Lumpur
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular cloning and molecular histochemistry of salmon olfactory marker protein in the cacustrine sockeye salmon (Oncorhynchus Nerka).2008

    • Author(s)
      Kudo, H., et al.
    • Organizer
      15^<th> International Symposium on Olfaction and Taste
    • Place of Presentation
      San Francisco
    • Related Report
      2008 Annual Research Report
  • [Presentation] Protective effects of an integrin-activating peptide, PHSRN, on peritoneal wound healing in a rat model acute peritoneal injury.2008

    • Author(s)
      Miyamoto, T., et al.
    • Organizer
      41th Annual Meeting of American Society of Nephrology
    • Place of Presentation
      Philadelphia
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラット小上皮におけるPKRの局在と経時的変化2007

    • Author(s)
      佐藤永洋, 他
    • Organizer
      第39回日本臨床分子形態学会
    • Place of Presentation
      甲府
    • Year and Date
      2007-09-28
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Book] 第7章内分泌系、第11章感覚器系、ビジュアル解剖生理学2007

    • Author(s)
      土肥良秋, 他
    • Publisher
      ヌーヴェルヒロカワ
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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