Investigation of ATP-induced chemotaxis in preadipocytes

• Project/Area Number: 19590207
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: OMATSU-KANBE Mariko Shiga University of Medical Science, 医学部, 准教授 (80161397)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 脂肪細胞 / 分化 / 細胞遊走 / 走化性 / ケモタキシス / ATP / P2受容体 / 増殖 / 前駆脂肪細胞 / 細胞接着 / 細胞分化

Research Abstract

マウスの前駆脂肪細胞である3T3-L1細胞を用い, その遊走性と生理的意義を検討した. 未分化脂肪細胞はATPに対して正の化学走性を示したが, 成熟脂肪細胞に遊走性を観察することはできなかった. 次に, マウスの腸管膜を細胞外基質として用いたところ, 細胞は約1時間で腸管膜表面に接着して移動を開始した.これらの結果から, 未分化脂肪細胞は生体内においても遊走性を持ち,オリジナルの脂肪組織塊から派生した細胞が離れた場所に移動して接着し, 増殖する可能性があると考えられた.

Research Products

• [Journal Article] A novel type of self-beatingcardiomyocytes in adult mouseventricles (2009)
  • Author(s): Omatsu-Kanbe, M. and Matsuura, H.
  • Journal Title: Biochem. Biophy.s Res. Commun 381 Pages: 361-366
  • Peer Reviewed
• [Journal Article] Swelling-Activated Cl-Current in Isolated Rabbit Articular Chondrocytes : Inhibition by Arachidonic Acid (2009)
  • Author(s): Isoya, E., Toyoda, F., Imai, S., Okumura,N., Kumagai, K., Omatsu-Kanbe, M.,Kubo, M., Matsuura, H., Matsusue, Y.
  • Journal Title: J Pharmacol Sci. 109 Pages: 293-304
  • Peer Reviewed
• [Journal Article] Angiotensin II type1 receptor mediates partiallyhyposmotic-induce increase of I_<Ks>current in guinea pig atrium (2009)
  • Author(s): Zankov, D. P., Toyoda, F.,Omatsu-Kanbe, M., Matsuura, H., Horie, M.
  • Journal Title: (In press)
  • Peer Reviewed
• [Journal Article] A novel type of self-beating cardiomyocytes in adult mouse ventricles. (2009)
  • Author(s): Omatsu, Kanbe, M., Matsuura, H
  • Journal Title: Biochem. Biophy. s Res. Commun. 381 Pages: 361-366
  • Peer Reviewed
• [Journal Article] Swelling-Activated Cl Current in Isolated Rabbit Articular Chondrocytes : Inhibition by Arachidonic Acid. (2009)
  • Author(s): Isoya, E., Toyoda, F., Imai, S., Okumura, N., Kumagai, K., Omatsu-Kanbe, M., Kubo, M., Matsuura, H., Matsusue, Y.
  • Journal Title: J Pharmacol Sci. 109 Pages: 293-304
  • Peer Reviewed
• [Journal Article] Angiotensin II type l receptor mediates partially hyposmotic-induce increase of IKs current in guinea pig atrium. (2009)
  • Author(s): Zankov, D. P., Toyoda, F., Omatsu-Kanbe, M., Matsuura, H., Horie, M.
  • Journal Title: Pflugers Arch. (in press)
  • Peer Reviewed
• [Journal Article] 心房筋伸展によるAT_1受容体を介した緩徐活性型遅延整流性K^+電流(I_<Ks>)の増大 ; 心房細動のARBによるアップストリーム治療のメカニズム (2008)
  • Author(s): 松浦博, Dimitar P. Zankov, 尾松万里子, 堀江稔
  • Journal Title: 分子血管病 9 Pages: 456-461
  • Peer Reviewed
• [Journal Article] 心房筋伸展によるAT_1受容体を介した緩徐活性型遅延整流性K^+電流(I_<Ks>)の増大 ; 心房細動のARBによるアツプストリーム治療のメカニズム (2008)
  • Author(s): 松浦博, Dimitar P. Zankov, 尾松万里子, 堀江稔
  • Journal Title: 分子心血管病 9 Pages: 456-461
  • Peer Reviewed
• [Journal Article] Inhibitory action of proteinkinase Cbeta inhibitor ontetrodotoxin-resistant Na^+ currentin small dorsal root ganglionneurons in diabetic rats (2007)
  • Author(s): Hayase, F., Matsuura H., Sanada M.,Kitada-Hamada K., Omatsu-Kanbe M.,Maeda, K., Kashiwagi, A. and Yasuda, H.
  • Journal Title: Neurosci Lett. 417 Pages: 90-94
  • Peer Reviewed
• [Journal Article] イオンチャネル異常による致死性不整脈 (2007)
  • Author(s): 松浦博, Dimitar P. Zankov, 尾松万里子, 礒野高敬, 豊田太, 丁維光, 堀江稔
  • Journal Title: 心房筋細胞におけるAT_1受容体を介した緩徐活性型遅延整流性K^+電流(I_<Ks>)の増大と活動電位の短縮-心房筋細胞治療におけるAT_<1>受容体遮断薬の有効性との関連-日本心電学会誌「心電図」 27 Pages: 145-153
  • Peer Reviewed
• [Journal Article] Inhibitory action of protein kinase Cbeta inhibitor on tetrodotoxin-resistant Na^+ current in small dorsal root ganglion neurons in diabetic rats (2007)
  • Author(s): F. Hayase
  • Journal Title: Neurosci Lett 417 Pages: 90-94
  • Peer Reviewed
• [Journal Article] イオンチャネル異常による致死性不整脈心房筋細胞におけるAT_1受容体を介した緩徐活性型遅延整流性K^+電流(Iks)の増大と活動電位の短縮-心房筋細胞治療におけるAT1受容体遮断薬の有効性との関連- (2007)
  • Author(s): 松浦 博
  • Journal Title: 日本心電学会誌「心電図」 27 Pages: 145-153
• [Presentation] Cell migration of preadipocytes on the isolated mouse mesentery. (2008)
  • Author(s): Omatsu, Kanbe, M., Pujii, Y, Matsuura, H.
  • Organizer: J. Physiol. Sci. 58, S202(第85回日本生理学会大会)
  • Place of Presentation: 東京
  • Year and Date: 2008-03-27
• [Presentation] Cell migration ofpreadipocytes on the isolated mousemesentery (2008)
  • Author(s): Omatsu-Kanbe, M., Fujii, Y & Matsuura, H.
  • Organizer: Journal of Physiological Sciences 58(第85回日本生理学会大会)
  • Place of Presentation: 東京(S202)
• [Presentation] Angiotensin II Type 1 Receptor Partially Mediates Hypoosmotic-Induced Increase of Guinea Pig Atrial I^<Ks> Current (2008)
  • Author(s): Zankov, D.P., Toyoda, F., Omatsu-Kanbe,M., Matsuura, H. & Horie, M.
  • Organizer: 52^<nd> Annual Meeting ofBiophysical Society
  • Place of Presentation: Long Beach, Florida,USA
• [Presentation] Angiotensin II Type 1 Receptor Partially Mediates Hypoosmotic-Induced Increase of Guinea Pig Atrial Iks Current (2008)
  • Author(s): Zankov, D. P.
  • Organizer: 52nd Annual Meeting of Biophysical Society
  • Place of Presentation: Long Beach, Florida, USA
• [Presentation] Cell migration of preadipocytes on the isolated mouse mesentery (2008)
  • Author(s): Omatsu-Kanbe, M.
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Extracellular ATP-induced cell migration of pradipocytes : Chemotaxis and chemokinesis (2007)
  • Author(s): Omatsu-Kanbe, M., Fujii, Y, & Matsuura, H.
  • Organizer: Journal of Physiological Sciences 57(第84回日本生理学会大会)
  • Place of Presentation: 大阪(S86)
• [Presentation] Role of phosphoproteins modified by the stimulation of P2Y receptor in adipocyte differentiation (2007)
  • Author(s): Fujii, Y., Omatsu-Kanbe, M., Isono, T. & Matsuura, H.
  • Organizer: Journal of Physiological Sciences 57(第84回日本生理学会大会)
  • Place of Presentation: 大阪(S125)
• [Presentation] 心房筋細胞におけるAT_1受容体を介した緩徐活性型遅延整流性K^+電流(I_<Ks>)の増大と活動電位の短縮-心房細動治療におけるAT_<1>重油対遮断薬の有効性との関連- (2007)
  • Author(s): 松浦博, Dimitar P. Zamkov, 尾松万里子, 礒野高敬, 豊田太, 丁維光, 堀江稔
  • Organizer: Japanese Journal of Electrocardiology(第23回日本心電学会シンポジウム)
  • Place of Presentation: 東京(7)(145-153)
• [Presentation] Angiotensin II Type 1 Receptor Partially Mediates Stretch-Induced Potentiation of the Slow Component of Delayed Rectifier Potassium Current in Guinea Pig Atrium (2007)
  • Author(s): Zankov, D.P., Toyoda, F., Omatsu-Kanbe,M., Matsuura, H. & Horie, M.
  • Organizer: Scientific Session 2007 of American Heart Association
  • Place of Presentation: Orland,Florida, USA
• [Presentation] Upregulation of HERG potassium channel function by phosphatidylinositol-4-phosphatase 5-kinase (2007)
  • Author(s): Kubo, T., Ding, W.-G., Toyoda, F., Fujii, Y., Omatsu-Kanbe, M., Miura, Y., Mino, T. & Matsuura, H.
  • Organizer: European Heart Journal
  • Place of Presentation: European Society of Cardiology Congress 2007, Vienna,Austria(28)
• [Presentation] Angiotensin II Type 1 Receptor Partially Mediates Stretch-Induced Potentiation of the Slow Component of Delayed Rectifier Potassium Current in Guinea Pig Atrium (2007)
  • Author(s): Zankov, D. P
  • Organizer: Scientific Session 2007 of American Heart Associati
  • Place of Presentation: Orland, Florida, USA
• [Presentation] Upregulation of HERG potassium channel function by phosphati dylinositol-4-phosphatase 5-kinase (2007)
  • Author(s): Kubo, T.
  • Organizer: European Heart Journal 28, 223-224(European Society of Cardiology Congress 2007)
  • Place of Presentation: Vienna, Austria