| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Osteoclasts resolve bone tissues by secreting acids and proteolytic enzymes and thus play significant roles in bone remodeling. We succeeded in a real-time measurements of proton currents through the plasma membrane vacuolar H^+-ATPase (V-ATPase) using the whole-cell clamp technique. The V-ATPase current was increased by intracellular acidification and depolarization, and inhibited rapidly by exposure to high concentrations of extracellular Ca^<2+>, a negative feedback signal for osteoclast functions. The underlying mechanisms of the Ca^<2+>-sensing responses were investigated.
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