Intercellular signaling by changes in the membrane potential of intracellular calcium stores

• Project/Area Number: 19590214
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Nara Medical University
• Principal Investigator: YAMASHITA Masayuki Nara Medical University, 医学部, 教授 (20183121)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞生理学 / 細胞内カルシウムストア / カルシウムオシレーション / 膜電位 / 電位感受性蛍光色素 / 共焦点蛍光顕微鏡 / 周波数スペクトラム / 光電子倍増管 / 網膜 / フォトマルチプライア / 細胞内電位記録 / 綱膜

Research Abstract

Synchronous Ca oscillation is caused by releases of Ca from intracellular Ca stores in retinal neuroepithelium. High-speed fluorescence measurement of store membrane potential revealed that periodic repeats of a burst of high-frequency voltage fluctuations occur in the store membrane potential. Electrical coupling across the cells may be mediated by the series capacitance of the store membrane and the plasma membrane.

Research Products

• [Journal Article] Synchronization of Ca2+ oscillations: Is gap junctional coupling sufficient (2010)
  • Author(s): Yamashita. M.
  • Journal Title: FEBS J. 277 Pages: 277-277
  • Peer Reviewed
• [Journal Article] Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium. (2010)
  • Author(s): Yamashita, M.
  • Journal Title: FEBS J. 277 Pages: 293-299
  • Peer Reviewed
• [Journal Article] Synchronization of Ca^<2+> oscillations : Is gap junctional coupling sufficient? (2010)
  • Author(s): Masayuki Yamashita
  • Journal Title: FEBS Journal VOL.277 Pages: 277-277
  • Peer Reviewed
• [Journal Article] Synchronization of Ca^<2+> oscillations : a capacitative(AC)electrical coupling model in neuroepithelium (2010)
  • Author(s): Masayuki Yamashita
  • Journal Title: FEBS Journal VOL.277 Pages: 293-299
  • Peer Reviewed
• [Journal Article] Voltage fluctuations of calcium store in retinal neuroepithelium (2009)
  • Author(s): Masayuki Yamashita
  • Journal Title: Journal of Physiological Sciences VOL.59 Pages: 149-149
  • Peer Reviewed
• [Journal Article] Synchronization of calcium oscillation between retinal neuroepithelial cells (2009)
  • Author(s): Masayuki Yamashita
  • Journal Title: Neuroscience Research VOL.65
  • Peer Reviewed
• [Journal Article] Synchronous Ca2+ oscillation emerges from voltage fluctuations of Ca2+ stores. (2008)
  • Author(s): Yamashita. M.
  • Journal Title: FEBS J. 275 Pages: 4022-4032
  • Peer Reviewed
• [Journal Article] Synchronous Ca^<2+> oscillation emerges from voltage fluctuations of Ca^<2+> stores (2008)
  • Author(s): Yamashita Masayuki
  • Journal Title: FEBS J. 275 Pages: 4022-4032
  • Peer Reviewed
• [Journal Article] Synchronization of calcium oscillation by fluctuations in the membranepotential of calcium store (2008)
  • Author(s): Yamashita Masayuki
  • Journal Title: The Journal of Physiological Sciences 58 Suppl.
  • Peer Reviewed
• [Presentation] 網膜神経上皮細胞間におけるカルシウムオシレーションの同期化メカニズム (2009)
  • Author(s): 山下勝幸
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] 網膜神経上皮細胞間におけるカルシウムオシレーションの同期化メカニズム (2009)
  • Author(s): 山下勝幸
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2009-09-17
• [Presentation] Voltage fluctuations of calcium store in retinal neuroepithelium. (2009)
  • Author(s): Masayuki Yamashita
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-28
• [Presentation] Voltage fluctuations of calcium store in retinal neuroepithelium (2009)
  • Author(s): 山下勝幸
  • Organizer: 36^<th> International Congress of Physiological Sciences
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-07-28
• [Presentation] 細胞内カルシウムストアの膜電位とカルシウムオシレーション解剖学・生理学連携シンポジウム"種々の細胞内現象と細胞内小器官に対する形態および機能面からのアプローチ" (2009)
  • Author(s): 山下勝幸
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-30
• [Presentation] 発生初期網膜の電気生理学的解析シンポジウム"網膜を材料にした多角的アプローチにより組織細胞の姿を知る" (2009)
  • Author(s): 山下勝幸
  • Organizer: 第114回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-29
• [Presentation] 発生初期網膜の電気生理学的解析 (2009)
  • Author(s): 山下勝幸
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山
• [Presentation] 細胞内カルシウムストアの膜電位とカルシウムオシレーション (2009)
  • Author(s): 山下勝幸
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山
• [Presentation] Synchronous calcium oscillation emerges from voltage fluctuations of calcium stores. (2008)
  • Author(s): Masayuki Yamashita
  • Organizer: The 9th International Congress on Cell Biology
  • Place of Presentation: Seoul
  • Year and Date: 2008-10-09
• [Presentation] カルシウムストアの膜電位変動によるカルシウムオシレーションの同期化 (2008)
  • Author(s): 山下勝幸
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] カルシウムストアの膜電位変動によるカルシウムオシレーションの同期化 (2008)
  • Author(s): 山下勝幸
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-26
• [Presentation] Synchronous calcium oscillation emerges from voltage fluctuations of calcium stores (2008)
  • Author(s): Yamashita Masayuki
  • Organizer: The 9^<th> International Congress on Cell Biology
  • Place of Presentation: Seoul