Mechanism of Nucleotide Binding Domain engine in ABC transporters
| Project/Area Number |
19590215
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Keio University (2008-2009)
Osaka Medical College (2007) |
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Principal Investigator |
SOHMA Yoshiro Keio University, 医学部, 准教授 (60268183)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Takahiro 大阪医科大学, 医学部, 教授 (10084906)
SOHMIYA Koichi 大阪医科大学, 医学部, 助教 (20319544)
YAMAMOTO Daisuke 大阪医科大学, 医学部, 准教授 (50240106)
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| Research Collaborator |
HWANG T.-c 米ミズーリ大学, 医学部, 教授
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ABCトランスポータ / CFTR / チャネル / Nucleotide Binding Domain / ATP加水分解 / ゲーティング / アニオン / 二量体化 / Nucleotide Bindin Domain / ATP 加水分解 / ゲーテイング |
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| Research Abstract |
Physiologically important ABC transporters share Nucleotide Binding Domain (NBD) to make a driving force for displaying their functions. From a series of functional data, we proposed a putative structure of the NBD engine of CFTR in one of the transitional states during its gating cycle. We also suggested a possibility that NBD engine utilized thermal motion energy of water molecules as well as ATP-hydrolysis energy. In addition, we obtained a reconstituted three dimensional structure of CFTR using a single particle analysis, which leads us to an upcoming direct observations of conformational changes of NBD engine.
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Report
(4 results)
Research Products
(23 results)
