Analysis of regulation by t-PA/t-PAR-mediate protease on regeneration system after tissue damage

• Project/Area Number: 19590217
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Kinki University
• Principal Investigator: MATSUO Osamu Kinki University, 医学部, 教授 (40030879)
• Co-Investigator(Kenkyū-buntansha): UESHIMA Shigeru 近畿大学, 農学部, 教授 (30193791) ; OKADA Kiyotaka 近畿大学, 医学部, 講師 (20185432) ; KAWAO Naoyuki 近畿大学, 医学部, 教授 (70388510)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 組織再生 / t-pA / t-PA受容体 / 線溶系 / 蛋白分解 / t-PA / タンパク分解

Research Abstract

作成したt-PAR発現線維芽細胞は、t-PA結合能とPlg活性化能が増強した。また、この細胞は37℃条件下の感温性ポリマー上で接着・増殖し、25℃でシート上に剥離した。以上の結果より、細胞表面上のt-PA/t-PAR系による蛋白分解活性を制御されたt-PAR発現細胞は、感温性ポリマー樹脂と組み合わせた新たな手法として組織損傷後の修復・再生への臨床応用が期待される。

Research Products

• [Journal Article] Binding of plasminogen to hepatocytes isolated from injured mouse liver and nonparenchymal-cell-dependent proliferation of hepatocytes (2008)
  • Author(s): Okada K, Ueshima S, Matsuo O, 他7名
  • Journal Title: Blood Coagul Fibrinolysis 19 Pages: 503-511
  • Peer Reviewed
• [Journal Article] The absence of uPAR attenuates insulin-induced vascular smooth muscle cell migration and proliferation (2008)
  • Author(s): Kanno Y, Ueshima S, Matsuo O, 他7名
  • Journal Title: Thromb Res 123 Pages: 336-341
  • Peer Reviewed
• [Journal Article] The absence of uPAR is associated with the progression of dermal fibrosis (2008)
  • Author(s): Kanno Y, Ueshima S, Matsuo O, 他7名
  • Journal Title: J Invest Dermatol 128 Pages: 2792-2797
  • Peer Reviewed
• [Journal Article] Alpha2-antiplasmin is a critical regulator of aneiotensin II-mediated vascular remodeling (2008)
  • Author(s): Hou Y, Okada K, Matsuo O, 他2名
  • Journal Title: Arterioscler Thromb Vasc Biol 28 Pages: 1257-1262
  • Peer Reviewed
• [Journal Article] VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats (2008)
  • Author(s): Li Y, Nagai N, Matsuo O, 他16名
  • Journal Title: J Clin Invest 118 Pages: 913-923
  • Peer Reviewed
• [Journal Article] Urokinase-type plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic stroke (2008)
  • Author(s): Nagai N, Okada K, Matsuo O, 他3名
  • Journal Title: Neurosci Lett 432 Pages: 46-49
  • Peer Reviewed
• [Journal Article] Unbalanced expression of ADAMTS13 and von Willebrand factor in mouse endotoxinemia (2008)
  • Author(s): Mimuro J, Okada K, Matsuo O, 他7名
  • Journal Title: Throm Res 122 Pages: 91-97
  • Peer Reviewed
• [Journal Article] 線溶系 : 血管外での新しい機能 (2008)
  • Author(s): 上嶋繁, 岡田清孝, 松尾理
  • Journal Title: 近畿大学医学会雑誌 33 Pages: 161-178
  • NAID: 120005736598
• [Journal Article] 肝再生と線溶系 (2008)
  • Author(s): 岡田清孝, 松尾理
  • Journal Title: 日本血栓止血学会誌 19 Pages: 216-225
  • NAID: 10026116063
• [Journal Article] 神経系における線溶系因子の役割 (2008)
  • Author(s): 永井信夫, 松尾理
  • Journal Title: Int Review Thromb 3 Pages: 22-28
• [Journal Article] VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats. (2008)
  • Author(s): Li Y
  • Journal Title: Journal of clinical Investigation 118 Pages: 913-923
  • Peer Reviewed
• [Journal Article] The absence of uPAR attenuates insulin-induced vascular smooth muscle cell migration and proliferation. (2008)
  • Author(s): Kanno Y
  • Journal Title: Thrombosis Research 123 Pages: 336-341
  • Peer Reviewed
• [Journal Article] Alpha2-antiplasmin is a critical regulator of angiotensin II-madiated vascular remodeling. (2008)
  • Author(s): Hou Y
  • Journal Title: Arterioscler Thrombosis Vascular Biology 28 Pages: 1257-1262
  • Peer Reviewed
• [Journal Article] The absence of uPAR is associated with the progression of dermal fibrosis. (2008)
  • Author(s): Kanno Y
  • Journal Title: Journal of Investigative Dermatology 128 Pages: 2792-2797
  • Peer Reviewed
• [Journal Article] Binding of plasminogen to hepatocytes isolated from injured mouse liver and nonparenchymal-cell-dependent proliferation of hepatocytes. (2008)
  • Author(s): Okada K
  • Journal Title: Blood Coagulation and Fibrinolysis 19 Pages: 503-511
  • Peer Reviewed
• [Journal Article] c-Myc is essential for urokinase plasminogen activator expression on hypoxia-induced vascular smooth muscle cells (2007)
  • Author(s): Hou Y, Okada K, Matsuo O, 他4名
  • Journal Title: Cardiovasc Res 75 Pages: 186-194
  • Peer Reviewed
• [Journal Article] A guide to fibrinolytic factor structure, function, assays, and genetic alterations (2007)
  • Author(s): Matsuo O, Lijnen HR, Ueshima S, 他2名
  • Journal Title: J Thromb Haemost 5 Pages: 680-689
  • Peer Reviewed
• [Journal Article] Effect of staphylokinase-derived nonadecapeptide on the activation of plasminogen (2007)
  • Author(s): Okada K, Ueshima S, Matsuo O, 他4名
  • Journal Title: Thromb Haemost 97 Pages: 795-802
  • Peer Reviewed
• [Journal Article] plasmin decreases the BH3-only protein BimEL via the ERK1/2 signaling pathway in hepatocytes (2007)
  • Author(s): Kawao N, Okada K, Matsuo O, 他4名
  • Journal Title: Biochim Biophys Acta- Mol Cell Res 1773 Pages: 718-727
  • Peer Reviewed
• [Journal Article] Mesenchymal progenitor cells in adult human dental pulp and their ability to form bone when transplanted into immunocompromised mice (2007)
  • Author(s): Otaki S, Ueshima S, Matsuo O, 他4名
  • Journal Title: Cell Biol Int 31 Pages: 1191-1197
  • Peer Reviewed
• [Journal Article] α_2-antiplasmin is involved in the production of transforming growth factor β1 and fibrosis (2007)
  • Author(s): Kanno Y, Okada K, Matsuo O, 他3名
  • Journal Title: J Thromb Haemost 5 Pages: 2266-2273
  • Peer Reviewed
• [Journal Article] Urokinase-type Plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic stroke (2007)
  • Author(s): Nagai N
  • Journal Title: Neurosci Lett (In press)
  • Peer Reviewed
• [Journal Article] Alpha2-antiplasmin is involved in the production of transforming growth factor beta1 and fibrosis (2007)
  • Author(s): Kanno Y
  • Journal Title: J Thromb Haemost 5 Pages: 2266-73
  • Peer Reviewed
• [Journal Article] Plasmin decreases the BH3-only protein BimEL via the ERK 1/2 sianaling Pathwav in hepatocytes (2007)
  • Author(s): Kawao N
  • Journal Title: Biochim Biophys Acta 1773 Pages: 718-727
  • Peer Reviewed
• [Presentation] 再生肝細胞上でのプロテアーゼ増幅系による肝再生制御機構の解析 (2009)
  • Author(s): 岡田清孝
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05
• [Presentation] 細胞性線溶と肝再生機構 (2008)
  • Author(s): 岡田清孝
  • Organizer: 第31回日本血栓止血学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-22
• [Presentation] 血管内皮細胞膜上でのt-PA binding proteinの発現とその線溶活性におよぼす影響 (2008)
  • Author(s): 石田知可子
  • Organizer: 第31回日本血栓止血学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2008-11-22
• [Presentation] 新規ペプチドのプラスミノーゲン活性化促進機序の解析 (2008)
  • Author(s): 岡田清孝
  • Organizer: 第31回日本血栓止血学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-21
• [Presentation] マウス再生肝細胞上における蛋白分解カスケード制御機構の解析 (2008)
  • Author(s): 岡田清孝
  • Organizer: 第31回日本血栓止血学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-21
• [Presentation] 血栓溶解療法の基礎 (2008)
  • Author(s): 上嶋繁
  • Organizer: 第31回日本血栓止血学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-21
• [Presentation] 光化学刺激血栓モデルによる局所的肝傷害後の修復過程におけるプラスミノゲンの役割 (2008)
  • Author(s): 河尾直之
  • Organizer: 第31回日本血栓止血学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-21
• [Presentation] The expression of urokinase type-plasminogen activator by c-Myc on hypoxia-induced vascular smooth muscle cells. (2008)
  • Author(s): Hou Y
  • Organizer: XIXth International Congress on Fibrinolysis and Proteolysis
  • Place of Presentation: Vienna
  • Year and Date: 2008-07-09
• [Presentation] 肝細胞上での蛋白分解カスケード増幅系による肝再生制御機構の解析 (2008)
  • Author(s): 岡田清孝
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-13
• [Presentation] 線溶系の肝再生過程への関与に対する肝細胞系での解析 (2008)
  • Author(s): 河尾直之
  • Organizer: 第15回日本病態生理学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-01-26
• [Presentation] Effect of synthetic peptides derived from staphylokinase on theactivation of plasminogen (2007)
  • Author(s): Okada K
  • Organizer: XXIst Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: ジュネーヴ
  • Year and Date: 2007-07-11
• [Presentation] Effect of synthetic peptides derived from staphylokinase on the activation of plasminogen (2007)
  • Author(s): Okada K.
  • Organizer: XXIst Congress of the International Society on Thrombosis and Haemotasis
  • Place of Presentation: Geneva
  • Year and Date: 2007-07-09
• [Book] Rele of fibrinolysis in hepatic regeneration. In: Recent Advances in Thrombosis and Hemostasis Tanaka K, Davie EW (eds) (2008)
  • Author(s): Okada K, Ueshima S, Matsuo O
  • Publisher: Tokyo, Springer Japan
• [Book] 凝固線溶系の新しい知見. In; 血栓塞栓症研究の新展開(編集:高杉善弘, 保田知生, 松尾理) (2007)
  • Author(s): 岡田清孝, 松尾理
  • Publisher: 大阪, 近畿大学出版
• [Patent(Industrial Property Rights)] 血栓症発症予防および治療用物質 (2008)
  • Inventor(s): 松尾理, 上嶋繁, 岡田清孝, 石田知可子, 白石浩平
  • Industrial Property Number: 2008-297312
  • Filing Date: 2008-10-24