Nicotine-induced remodeling of synapse in hippocampal neuron.

• Project/Area Number: 19590247
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Osaka University
• Principal Investigator: TANAKA Hidekazu Osaka University, 大学院・医学系研究科, 准教授 (70273638)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 中枢神経 / ニコチン / シナプス / 可塑性 / スパイン / 認知症 / 薬物依存 / 海馬

Research Abstract

Nicotine was discovered to induce synaptic remodeling. The remodeling of dendritic spine, the postsynaptic structure, was visualized with green fluorescent protein (GFP) in cultured hippocampal neurons. One-two hours after the application of nicotine, a several micrometer-long filopodia protruded from the head of mushroom-shaped spine. This unique remodeling of spine head suggests a kind of the destabilization of synaptic connection, which may result in the rewiring of neural circuit. This phenomenon might be one of the mechanisms underlying the nicotine addiction.

Research Products

• [Journal Article] Retrospective review of Japanese sudden unexpected death in infancy: the importance of metabolic autopsy and expanded newborn screening. (2011)
  • Author(s): Yamamoto T, Tanaka H, Kobayashi H, Okamura K, Tanaka T, Emoto Y, Sugimoto K, Nakatome M, Sakai N, Kuroki H, Yamaguchi S, Matoba R.
  • Journal Title: Mol Genet Metab. 102 Pages: 399-406
  • Peer Reviewed
• [Journal Article] Non-clustered Protocadherin. (2011)
  • Author(s): Kim SY, Yasuda S, Tanaka H, Yamagata K, Kim H.
  • Journal Title: Cell Adh Migr. volume 5, issue 2, in press
  • Peer Reviewed
• [Journal Article] p38 Map Kinase Inhibitors as Potential Therapeutic Drugs for Neural Diseases. (2011)
  • Author(s): Yasuda S, Sugiura H, Tanaka H, Takigami S, Yamagata K.
  • Journal Title: Cent Nerv Syst Agents Med Chem. 11 Pages: 45-59
  • Peer Reviewed
• [Journal Article] Retrospective review of Japanese sudden unexpected death in infancy : the importance of metabolic autopsy and expanded newborn screening. (2011)
  • Author(s): Yamamoto T, Tanaka H, et al.
  • Journal Title: Mol Genet Metab Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] A novel transporter of SLC22 family specifically transports prostaglandins and co-localizes with 15-hydroxyprostaglandin dehydrogenase in renal proximal tubules. (2010)
  • Author(s): Shiraya K, Hirata T, Hatano R, Nagamori S, Wiriyasermkul P, Jutabha P, Matsubara M, Muto S, Tanaka H, Asano S, Anzai N, Endou H, Yamada A, Sakurai H, Kanai Y.
  • Journal Title: J Biol Chem. 285 Pages: 22141-22151
  • Peer Reviewed
• [Journal Article] A novel transporter of SLC22 family specifically transports prostaglandins and co-localizes with 15-hydroxyprostaglandin dehydrogenase in renal proximal tubules. (2010)
  • Author(s): Shiraya K, ---Tanaka H, et al.
  • Journal Title: J Biol Chem. Volume: 285 Pages: 22141-22151
  • Peer Reviewed
• [Journal Article] p38 Map Kinase Inhibitors as Potential Therapeutic Drugs for Neural Diseases. (2010)
  • Author(s): Yasuda S, Sugiura H, Tanaka H, et al.
  • Journal Title: Cent Nerv Syst Agents Med Chem. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Non-clustered Protocadherin. (2010)
  • Author(s): Kim SY, Yasuda S, Tanaka H, et al.
  • Journal Title: Cell Adh Migr. Volume: 5(印刷中)
  • Peer Reviewed
• [Journal Article] Methanphetamine directly accelerates beating rate in cardiomyocytes by increasing Ca2+ entry via L-type Ca2+ channel. (2009)
  • Author(s): Sugimoto K, Okamura K, Tanaka H, Takashima S, Ochi H, Yamamoto T, Matoba R.
  • Journal Title: Biochem Biophys Res Commun. 390 Pages: 1214-1220
  • Peer Reviewed
• [Journal Article] N-cadherin regulates differentiation of neural precursor cells. (2009)
  • Author(s): Yagita Y, Sakurai T, Tanaka H, Kitagawa K, Colman DR, Shan W.
  • Journal Title: J Neurosci Res. 87 Pages: 3331-3342
  • Peer Reviewed
• [Journal Article] Transducing Neuronal Activity into Dendritic Spine Morphology: New Roles for p38 MAP Kinase and N-Cadherin. (2009)
  • Author(s): Sugiura H, Tanaka H, Yasuda S, Takemiya T, Yamagata K.
  • Journal Title: Neuroscientist. 15 Pages: 90-104
  • Peer Reviewed
• [Journal Article] A novel role of the C-terminus of b 0,+ AT in the ER-Golgi trafficking of the rBAT-b 0,+ AT heterodimeric amino acid transporter. (2009)
  • Author(s): Sakamoto S, Chairoungdua A, Nagamori S, Wiriyasermkul P, Promchan K, Tanaka H, Kimura T, Ueda T, Fujimura M, Shigeta Y, Naya Y, Akakura K, Ito H, Endou H, Ichikawa T, Kanai Y.
  • Journal Title: Biochem J. 417 Pages: 441-448
  • Peer Reviewed
• [Journal Article] N-cadherin regulates differentiation of neural precursor cells (2009)
  • Author(s): Yagita Y, Sakurai T, Tanaka H, Kitagawa K, Colman DR, Shan W.
  • Journal Title: J Neurosci Res. 87 Pages: 3331-42
  • Peer Reviewed
• [Journal Article] Transducing Neuronal Activity into Dendritic Spine Morphology : New Roles for p38 MAP Kinase and N-Cadherin (2009)
  • Author(s): Sugiura H, Tanaka H, Yasuda S, Takemiya T, Yamagata K
  • Journal Title: Neuroscientist 15 Pages: 90-104
  • Peer Reviewed
• [Journal Article] N-cadherin regulates differentiation of neural precursor cells (2009)
  • Author(s): Yagita Y, et al.
  • Journal Title: J Neurosci Res 87 Pages: 3331-3342
  • Peer Reviewed
• [Journal Article] Methanphetamine directly accelerates beating rate in cardiomyocytes by increasing Ca2+ entry via L-type Ca2+ channel (2009)
  • Author(s): Sugimoto K, et al.
  • Journal Title: Biochem Biophys Res Commun 390 Pages: 1214-1220
  • Peer Reviewed
• [Journal Article] Transducing Neuronal Activity into Dendritic Spine Morphology : New Roles for p38 MAP Kinase and N-Cadherin. (2009)
  • Author(s): Sugiura H, et al.
  • Journal Title: Neuroscientist 15 Pages: 90-104
  • Peer Reviewed
• [Journal Article] A novel role of the C-terminus of b 0, + AT in the ER-Golgitrafficking of the rBAT-b 0, + AT heterodimeric amino acid transporter. (2009)
  • Author(s): Sakamoto S, et al.
  • Journal Title: Biochem J 417 Pages: 441-448
  • Peer Reviewed
• [Journal Article] N-cadherin regulates differentiation of neural precursor cells. (2009)
  • Author(s): Yagita Y, et al.
  • Journal Title: J Neurosci Res in press
  • Peer Reviewed
• [Journal Article] L-Glutamate enhances methylmercury toxicity by synergistically increasing oxidative stress. (2008)
  • Author(s): Amonpatumrat S, Sakurai H, Wiriyasermkul P, Khunweeraphong N, Nagamori S, Tanaka H, Piyachaturawat P, Kanai Y.
  • Journal Title: J Pharmacol Sci. 108 Pages: 280-289
  • NAID: 10024593004
  • Peer Reviewed
• [Journal Article] ニューロンの過剰興奮後に生じるシナプス減少のメカニズム. (2008)
  • Author(s): 田中秀和、安田新、杉浦弘子、山形要人
  • Journal Title: 実験医学 26(8) Pages: 1257-1260
• [Journal Article] L-Glutamate enhances methylmercury toxicity by synergistically increasing oxidative stress. (2008)
  • Author(s): Amonpatumrat S, et al.
  • Journal Title: J Pharmacol Sci 108 Pages: 208-289
  • Peer Reviewed
• [Journal Article] Synaptic Activity-induced Protocadherin Arcadlin Regulates Dendritic Spine Number by Triggering N-Cadherin Endocytosis via TAO2β and p38 MAP Kinases. (2007)
  • Author(s): Yasuda S, Tanaka H, Sugiura H, Okamura K, Sakaguchi T, Tran U, Takemiya T, Mizoguchi A, Yagita Y, Sakurai T, De Robertis EM, Yamagata K.
  • Journal Title: Neuron. 56 Pages: 456-471
  • Peer Reviewed
• [Journal Article] Sema4D-plexin-B1 implicated in regulation of dendritic spine density through RhoA/ROCK pathway. (2007)
  • Author(s): Lin X, Ogiya M, Takahara M, Yamaguchi W, Furuyama T, Tanaka H, Tohyama M, Inagaki S.
  • Journal Title: Neurosci Lett. 428 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Granulocyte colony-stimulating factor has a negative effect on stroke outcome in a murine model. (2007)
  • Author(s): Taguchi A, Wen Z, Myojin K, Yoshihara T, Nakagomi T, Nakayama D, Tanaka H, Soma T, Stern DM, Naritomi H, Matsuyama T.
  • Journal Title: Eur J Neurosci. 26 Pages: 126-33
  • Peer Reviewed
• [Journal Article] Synaptic Activity-induced Protocadherin Arcadlin Regulates Dendritic Spine Number by Triggering N-Cadherin Endocytosis via TAO2β and p38 MAP Kinases (2007)
  • Author(s): Yasuda S, Tanaka H, Sugiura H, Okamura K, Sakaguchi T, Tran U, Takemiya T, Mizoguchi A, Yagita Y, Sakurai T, De Robertis EM, Yamagata K
  • Journal Title: Neuron 56 Pages: 456-71
  • Peer Reviewed
• [Journal Article] Granulocyte colony-stimulating factor has a negative effect on stroke ou tcome in a muririe model (2007)
  • Author(s): Taguchi A
  • Journal Title: Eur J Neurosci 26 Pages: 126-133
  • Peer Reviewed
• [Journal Article] Sema4D-plexin-B1 implicated in regulation of dendritic spine density through RhoA/ROCK pathway (2007)
  • Author(s): Lin X
  • Journal Title: Neurosci Lett 428 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Synaptic activity-induced protocadherin arcadlin regulates dendritic spine number by triggering N-cadherin endocytosis via TAO2β and p38 MAP kinases (2007)
  • Author(s): Yasuda S
  • Journal Title: Neuron 56 Pages: 456-471
  • Peer Reviewed
• [Presentation] 海馬神経細胞接着分子N-cadherin結合蛋白質の網羅的解析 (2010)
  • Author(s): 田中秀和, ほか
  • Organizer: 第118回日本薬理学会近畿部会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-19
• [Presentation] 海馬神経細胞接着分子N-cadherin結合蛋白質の網羅的解析 (2010)
  • Author(s): 田中秀和, ほか
  • Organizer: 第118回 日本薬理学会近畿部会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-19
• [Presentation] Dynamic roles of proteins in synaptic adherens junction. (2010)
  • Author(s): 田中秀和, et al.
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
• [Presentation] シナプスリモデリングの情報伝達経路の探索 (2009)
  • Author(s): 田中秀和
  • Organizer: 日本薬理学会近畿部会
  • Place of Presentation: 石川
  • Year and Date: 2009-06-26
• [Presentation] シナプスリモデリングの情報伝達経路の探索 (2009)
  • Author(s): 田中秀和
  • Organizer: 日本薬理学会近畿部会
  • Place of Presentation: 石川県金沢市ホテル金沢
  • Year and Date: 2009-06-26
• [Presentation] Dynamic roles of proteins in synaptic adherens junction. (2009)
  • Author(s): 田中秀和, et al.
  • Organizer: 36th International congress of physiological sciences.
  • Place of Presentation: 京都
• [Presentation] Dynamic roles of proteins in synaptic adherens junction (2009)
  • Author(s): Tanaka H
  • Organizer: 36^<th> International congress of physiological sciences
  • Place of Presentation: Kyoto
• [Presentation] Dynamic roles of proteins in synaptic adherens junction (2009)
  • Author(s): 田中秀和, et al.
  • Organizer: 36^<th> International congress of physiological sciences
  • Place of Presentation: 京都国際会議場