Analysis of the regulation of organelle-generated signals by the Novel endoplasmic protein calumin

• Project/Area Number: 19590267
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: YAMAZAKI Tetsuo Kyoto University, 薬学研究科, 准教授 (90330208)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: カルシウム / 小胞体 / 細胞内小器官 / シグナル

Research Abstract

研究者代表者はCa^(2+)結合能を有する小胞体タンパク質caluminをモデル分子として、小胞体発シグナルの分子論的理解を目指した.樹立したcalumin欠損マウスの75%は胎児期に死亡した. 背景に体液循環異常が示唆され、実際、同胎児の心血管系形成は未熟な段階で停止していた. また、Ca^(2+)依存性転写因子NFATの活性化が阻害されており、NFATc3/c4二重欠損マウスとの類似性(胎生致死、心血管異常)を鑑みると、caluminとNFATの機能共役が小胞体発シグナルの分子基盤の一端を成すと考えられた.

Research Products

• [Journal Article] New molecular components supporting ryanodine receptor-mediated Ca^<2+> release : roles of junctophilin and TRIC channel in embryonic cardiomyocyes (2009)
  • Author(s): Yamazaki D., Yamazaki T. and Takeshima H
  • Journal Title: Pharmacol. Therapeut 121 Pages: 265-272
  • Peer Reviewed
• [Journal Article] Mitsugumin 53-mediated maintenance of K+ currents in cardiac myocytes (2009)
  • Author(s): Masumiya H., Asaumi Y., Nishi M., Minamisawa S., Adachi-Akahane S., Yoshida M., Kangawa K., Ito K., Kagaya Y., Yanagisawa T., Yamazaki T. , Ma J. and Takeshima H
  • Journal Title: Channels 3 Pages: 6-11
  • Peer Reviewed
• [Journal Article] Mitsugumin 53-mediated maintenance of K+ currents incardiac myocytes (2009)
  • Author(s): Masumiya H.
  • Journal Title: Channels 3 Pages: 6-11
  • Peer Reviewed
• [Journal Article] New molecular components supporting ryanodine receptor-mediated Ca2+ release : roles of junctophilin and TRIC channel in embryonic cardiomyocyes (2009)
  • Author(s): Yamazaki D.
  • Journal Title: Pharmacol. Ther. 121 Pages: 265-272
  • Peer Reviewed
• [Journal Article] Enhanced NK-cell development and function in BCAP-deficient mice (2008)
  • Author(s): MacFarlane IV AW., Yamazaki T., Fang M., Sigal LJ., Kurosaki T. and Campbell KS
  • Journal Title: Blood 112 Pages: 131-140
  • Peer Reviewed
• [Journal Article] Regulation of B cell development by BCAP and CD19 through their binding to phosphoinositide 3-kinase (2008)
  • Author(s): Aiba Y., Kameyama M., Yamazaki T., Tedder TF. And Kurosaki T
  • Journal Title: Blood 111 Pages: 1497-150
  • Peer Reviewed
• [Journal Article] Regulation of B cell development by BCAP and CD19 through their binding to phosphoinositide 3-kinase (2008)
  • Author(s): Aiba Y.
  • Journal Title: Blood 111 Pages: 1497-1503
  • Peer Reviewed
• [Journal Article] Enhanced NK-cell development and function in BCAP-deficient mice (2008)
  • Author(s): MacFarlane IV AW.
  • Journal Title: Blood 112 Pages: 131-140
  • Peer Reviewed
• [Journal Article] Abnormal features in mutant cerebellar Purkinje cells lacking junctophilins. Biochem. Biophys. Res (2007)
  • Author(s): Ikeda A., Miyazaki T., Kakizawa S., Okuno Y., Tsuchiya S., Myomoto A., Saito S., Yamamoto T., Yamazaki T., Iino M., Tsujimoto G., Watanabe M. and Takeshima H
  • Journal Title: Commun 363 Pages: 835-839
  • Peer Reviewed
• [Journal Article] Augmentation of drug-induced cell death by ER protein BRI3BP. Biochem. Biophys. Res (2007)
  • Author(s): Yamazaki T. , Sasaki N., Nishi M., Yamazaki D., Ikeda A., Okuno Y., Komazaki S. and Takeshima H
  • Journal Title: Commun 362 Pages: 971-975
  • Peer Reviewed
• [Journal Article] Calumin, a novel Ca2+ -binding protein on the endoplasmic reticulum (2007)
  • Author(s): Zhang M., Yamazaki T. , Yazawa M., Treves S., Nishi M., Murai M., Shibata E., Zorzato F. and Takeshima H
  • Journal Title: Cell Calcium 42 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Calumin, a novel Ca2+-binding transmembrane protein on the endoplasmic reticulum. (2007)
  • Author(s): Zhang, M.
  • Journal Title: Cell Calcium 42 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Augmentation of drug-induced cell death by ER protein BRI3BP (2007)
  • Author(s): Yamazaki, T.
  • Journal Title: Biochem Biophys Res Commun. 362 Pages: 971-975
  • Peer Reviewed
• [Journal Article] Abnormal features in mutant cerebellar Purkinje cells lacking junctophilins. (2007)
  • Author(s): Ikeda, A.
  • Journal Title: Biochem Biophys Res Commun. 363 Pages: 835-839
  • Peer Reviewed
• [Presentation] 小胞体蛋白質MG23を介した小胞体発シグナルと細胞死 (2007)
  • Author(s): 山崎 哲男
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Book] 医歯薬系学生のためのillustrated基礎生命科学 (2008)
  • Author(s): 竹島浩、山崎哲男、池田篤史、輿水崇鏡
  • Publisher: 京都廣川書店
• [Book] 医歯薬系学生のためのillustrated基礎生命科学 (2008)
  • Author(s): 竹島浩
  • Total Pages: 241
  • Publisher: 京都廣川書店