Function of Wnt signal in the formation of synapse

• Project/Area Number: 19590279
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kagoshima University
• Principal Investigator: KISHIDA Shosei Kagoshima University, 大学院・医歯学総合研究科, 教授 (50274064)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: シグナル伝達 / 分子間相互作用 / Wnt / シナプス形成 / Dvl / グリオーマ細胞 / シナプス / ユビキチンリガーゼ / HECT / Wnt-7a / GSK3

Research Abstract

シナプス形成におけるWnt-7aの作用機構の解析をするために、Wnt-7a蛋白質の発現系の確立を行った。マウスL細胞においてWnt-7a蛋白質を培養上清中に分泌する細胞株を樹立した。新規Dvl結合蛋白質候補Hect型ユビキチンリガーゼの全長cDNAをクローニングして、動物細胞で発現ベクターを構築してDvlとの哺乳動物細胞内での分子間相互作用を解析したが、有意な複合体形成は認められなかった。

Research Products

• [Journal Article] Identification of 13 novel mutations including a retrotransposal insertion in SLC25A13 gene and frequency of 30 mutations found in patients with citrin deficiency. (2008)
  • Author(s): Tabata A., Sheng JS., Ushikai M., Song YZ., Gao HZ., Lu YB., Okumura F., Iijima M., Mutoh K., Kishida S., Saheki T. and Kobayashi K
  • Journal Title: J. Hum. Genet. 53巻 Pages: 534-545
  • NAID: 80019634107
  • Peer Reviewed
• [Journal Article] Identification of 13 novel mutat ions including a retrotransposal insertion in SLC25A13 gene and frequency of 30 Mutations found in patients with citrin deficiency. (2008)
  • Author(s): Tabata A., et al
  • Journal Title: J. Hum. Genet. 53 Pages: 534-545
  • Peer Reviewed
• [Journal Article] Dvl regulates endo- and exocytotic prosesses through binding to synaptotagmin. (2007)
  • Author(s): Kishida S., Hamao K., Inoue M., Hasegawa Kikuchi A., Yamamoto H. and Kishida S. M., Matsuura Y., Mikoshiba K., Fukuda M. and Kikuchi A
  • Journal Title: Genes Cells 12巻 Pages: 49-61
  • Peer Reviewed
• [Journal Article] (2007)
  • Author(s): Kikuchi A., Yamamoto H. and Kishida S.
  • Journal Title: Cell Signal 19巻 Pages: 659-671
  • Peer Reviewed
• [Journal Article] Dvl regulates endo- and exocytotic processes through binding to synaptotagmin (2007)
  • Author(s): Kishida S.他
  • Journal Title: Genes Cells 12 Pages: 49-61
  • Peer Reviewed
• [Journal Article] Multiplicity of the interactions of Wnt proteins and their rece ptors (2007)
  • Author(s): Kikuchi A.他
  • Journal Title: Cell Signal. 19 Pages: 659-671
  • Peer Reviewed