Cell characteristic analysis in cultured human neural stem cells and development of the evaluation system

• Project/Area Number: 19590287
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Keio University
• Principal Investigator: YASUO Nagai Keio University, 医学部, 助教 (10425704)
• Co-Investigator(Kenkyū-buntansha): MATSUZAKI Yumi 慶應義塾大学, 医学部, 准教授 (50338183)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 再生医学 / 神経幹細胞 / 分化

Research Abstract

本研究は、高率に神経へ分化する神経幹細胞のマーカーを同定することによって分化傾向の違う神経幹細胞の精製を目指した。ヒト神経幹細胞を様々な細胞表面特異的抗原に対するモノクローナル抗体で染色後、サブポピュレーションをフローサイトメーターにより分離した。また、CD133陽性ヒト神経幹細胞の中でもCD24 陰性細胞が、CD24陽性細胞やフローサイトメーターで分離していない細胞に比べて自己増殖能に優れていることを明らかにした。

Research Products

• [Journal Article] Development of mesenchymal stem cells partially originate from the neural crest. (2009)
  • Author(s): Morikawa S, Mabuchi Y, Niibe K, Suzuki S, Nagoshi N, Sunabori T, Shimmura S, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y.
  • Journal Title: Biochem Biophys Res Commun. 379(4) Pages: 1114-1119
  • Peer Reviewed
• [Journal Article] Development of mesenchymal stem cells partially originate from the neural crest. (2009)
  • Author(s): Satoru Morikawa, et al.
  • Journal Title: Biochem Biophys Res Commun 379(4) Pages: 1114-1119
  • Peer Reviewed
• [Journal Article] Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad (2008)
  • Author(s): Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E, Morikawa S, Okada Y, Mabuchi Y, Katoh H, Okada S, Fukuda K, Suda T, Matsuzaki Y, Toyama Y, Okano H.
  • Journal Title: Cell Stem Cell. 2(4) Pages: 392-403
  • Peer Reviewed
• [Journal Article] Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad. (2008)
  • Author(s): Narihito Nagoshi, et al.
  • Journal Title: Cell Stem Cell 2(4) Pages: 392-403
  • Peer Reviewed
• [Presentation] Effect of low oxygen environment on cultured human fetal neural stem/progenitor cells (2008)
  • Author(s): Yasuo Nagai
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-10