Lipidomic analysis of the action of Phospholipase B/Lipase on intestinal mucosal cell differentiation

• Project/Area Number: 19590307
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Osaka University
• Principal Investigator: TOJO Hiromasa Osaka University, 生命機能研究科, 准教授 (90135707)
• Co-Investigator(Renkei-kenkyūsha): KONDOH Gen 京都大学, 再生医科学研究所, 准教授 (40243258)
• Research Collaborator: TAKEMURA Hiromasa 大阪大学, 生命機能研究科, 大学院生
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: リピドミクス / ホスホリパーゼB / リパーゼ / 小腸 / 消化吸収 / 分化

Research Abstract

今回新たに作成した小腸刷子縁膜に局在するCa非依存性ホスホリパーゼB/リパーゼ(PLB/LIP)欠損マウス(mPLB-/-)を用いて同酵素の機能解析をした。経口脂質負荷試験では野生型とmPLB-/-で体重・血清脂質値などに差がなかった。回腸Ca^<2+>依存性ホスホリパーゼA2活性が代償的に増加していたことから、PLB/LIPの脂質消化機能を代償する酵素が働いていることが示唆された。最初に作成したmPLB+/-で見られた分化異常はベクター由来のプロモータの影響と考えられた。中性脂質、中性スフィンゴ脂質、リン脂質にわたる自動化脂質一斉分析システムの測定可能脂質クラス範囲を、小腸に多い中性・酸性糖脂質を含む高極性脂質まで拡張できた。

Research Products

• [Journal Article] Cbesity causes a shift in metabolic flow of gangliosides in adipose tissues (2009)
  • Author(s): Tanabe, A., Mgt suds, M, Fukuhara, A., Myata, Y., Komuro, R, Shimomura, I., Tojo, H.
  • Journal Title: Biochem Biophy.Res.Commun 379 Pages: 547-552
  • Peer Reviewed
• [Journal Article] Obesity causes a shift in metabolic flow of gangliosides in adipose tissues. (2009)
  • Author(s): Tanabe, A
  • Journal Title: Biochem. Biophy. Res. Commnun. 379 Pages: 547-552
  • Peer Reviewed
• [Journal Article] Impaired TCR signaling through dysfunction of lipid rafts in sphingomyelin synthase 1 (SNS1)-knockdown T cells (2008)
  • Author(s): Jin, ZX, Huang, CR, Dong, L., Goda, S., Kawanami T., Sawaki T., Sakai T., Tong XP., Masaki Y., Fukushima T., Tanaka M, Mimori T., Tojo, H., Bloom ET., Ckazaki T., Umehara, H.
  • Journal Title: Int.Immunol 20 Pages: 1427-1437
  • Peer Reviewed
• [Journal Article] 2-Hydroxy- ceramide synthesis by ceramide synthase family : enzymatic basis for the preference of FA chain length. (2008)
  • Author(s): Mizutani, Y., Kihara, A., Chiba, H., Tojo, H., Igarashi, Y.
  • Journal Title: J.Lipid Res. 49 Pages: 2356-2364
  • Peer Reviewed
• [Journal Article] Impaired TCR signaling through dysfunction of lipid rafts in sphingowyelin synthase 1 (SMS1)-knockdown T cells (2008)
  • Author(s): Jin, ZX
  • Journal Title: Int Immunol. 20 Pages: 1427-1437
  • Peer Reviewed
• [Journal Article] 2-Hydroxy-ceramide synthesis by ceramide synthase family : enzy matic basis for the preference of FA chain length (2008)
  • Author(s): Mizutani, Y.
  • Journal Title: J Lipid Res. 49 Pages: 2356-2364
  • Peer Reviewed
• [Journal Article] Molecular Characterization of Menbrane-Associated Soluble Serine Palnitoyltransferases from Sphingobacterium multivorun, Sphingobacterium spiritivorun and Bdellovibriostolpii (2007)
  • Author(s): Ikushiro, H., Islam, M., Tojo, H., Hayashi, H.
  • Journal Title: J.Bactriol 189 Pages: 5749-5761
  • Peer Reviewed
• [Journal Article] Molecular Characterization of Membrane-Associated Soluble Serine Palmitoyltransferases from Sphingobacterium multivorun, Sphingobacterium spiritivorun and Bdellovibrio stolpii. (2007)
  • Author(s): Ikushiro, H
  • Journal Title: J. Bactriol. 189 Pages: 5749-5761
  • Peer Reviewed
• [Presentation] Lipidomics shows that obesity causes a shiftin steady-state metabolic flow of gangliosides downstream from GMB in adipose tissues of murine genetic and nutritional disease models (2009)
  • Author(s): Tojo, H., Tanabe, A.
  • Organizer: 2009 ASMS Sanibel conference
  • Place of Presentation: フロリダ米国
  • Year and Date: 2009-01-24
• [Presentation] 遺伝子変異マウスを用いたボスホリパーゼB/リパーゼの生理的機能の解析 (2008)
  • Author(s): 竹村浩昌, 東城博雅, 近藤玄
  • Organizer: BMB 2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 肥満脂肪組織におけるスフィンゴ糖脂質代謝の変化-HPLC/質量分析による解析 (2008)
  • Author(s): 田邉文, 福原淳範, 下村伊一郎, 東城博雅
  • Organizer: BMB 2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 遺伝子変異マウスを用いたホスホジパーゼB/リパーゼの生理的機能の解析 (2008)
  • Author(s): 竹村浩昌
  • Organizer: BMB2008
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-09
• [Presentation] Abeneficial effect of polyphenol oligomers, oligonol, on choline deficiency induced fatty liver in nice (2008)
  • Author(s): Tojo, H. Igura, T., Nbnomura, S., Sumi, R., Bando, Y., Ito. T.
  • Organizer: 49th International Conference on the Bioscience of Lipids
  • Place of Presentation: マーストリヒト、オランダ
  • Year and Date: 2008-08-29
• [Presentation] A Beneficial Effect of Polyphenol Oligomers, Oligonol, on Choline deficiency-induced Fatty Liver in mice. (2008)
  • Author(s): Hiromasa Tojo
  • Organizer: 49th International Conference on the Bioscience of Lipids
  • Place of Presentation: Maastricht University Netherlands
  • Year and Date: 2008-08-29
• [Presentation] Sphingolipidomic Profiling of the Stratum Corneum of Mice with Keratinocyte-specific Deletion of Aryl Hydrocarbon Receptor Nuclear Translocator Gene (2008)
  • Author(s): Tojo, H.
  • Organizer: 56th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: デンバー米国
  • Year and Date: 2008-06-04
• [Presentation] 遺伝子変異マウスを用いたボスホリパーゼB/リパーゼの生理的機能の解析 (2007)
  • Author(s): 竹村浩昌, 東城博雅, 近藤玄
  • Organizer: 第80会日本生化学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] 遺伝子変異マウスを用いたホスホリパーゼB/リパーゼの生理的機能の解析 (2007)
  • Author(s): 竹村 浩昌
  • Organizer: 第80会日本生化学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] Lipidomic analysis based on normal-phase HPLC/mass spectrometry (2007)
  • Author(s): Tojo, H.
  • Organizer: 5th Euro Fed Lipid Congress
  • Place of Presentation: ヨーテポリ、スエーデン
  • Year and Date: 2007-09-17
• [Presentation] Lipidomic analysis based on normal-phase HPLC/mass spectrometry (2007)
  • Author(s): Tojo, H
  • Organizer: 5th Euro Fed Lipid Congress
  • Place of Presentation: ヨーテボリ、スエーデン
  • Year and Date: 2007-09-17
• [Presentation] 48th International Conference on the Bioscience of Lipids Profiling of stratumcorneumlipids by an HPLC/mass spectrometry-based automatedlipidanalyzer (2007)
  • Author(s): Tojo, H.
  • Place of Presentation: ツルク、ブインランド
  • Year and Date: 2007-09-07
• [Presentation] Profiling of stratum corneum lipids by an HPLC/mass spectrometry-based automated lipid analyzer (2007)
  • Author(s): Tojo, H
  • Organizer: 48th International Conference on the Bioscience of Lipids
  • Place of Presentation: ツルク、フィンランド
  • Year and Date: 2007-09-07
• [Book] 自動化脂質分析装置を用いた病態リピドミクス"メタポロミクスの先端技術と応用"第20章 (2008)
  • Author(s): 東城博雅
  • Publisher: シーエスシー出版
• [Book] メタボロミクスの先端技術と応用 (2008)
  • Author(s): 東城 博雅
  • Publisher: シーエスシー出版
• [Book] 質量分析学 (2008)
  • Author(s): 東城 博雅
  • Publisher: 大阪大学出版会(印刷中)
• [Book] リピドミクス質量分析学9章
  • Author(s): 東城博雅
  • Publisher: 大阪大学出版会(印刷中)