Biological formation of 8-nitroguanosine 3'5'-cyclic monophosphate and its implication in NO signaling
| Project/Area Number |
19590312
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
SAWA Tomohiro Kumamoto University, 大学院・医学薬学研究部, 准教授 (30284756)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Tatsuya 熊本大学, 大学院・医学薬学研究部, 助教 (30419634)
AKAIKE Takaaki 熊本大学, 大学院・医学薬学研究部, 教授 (20231798)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 活性酸素 / 酸化ストレス / 一酸化窒素 / レドックス / シグナル伝達 / 適応応答 |
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| Research Abstract |
新規な環状ヌクレオチドである8-ニトログアノシン3', 5'-サイクリック一リン酸(8-ニトロcGMP)は蛋白質チオール基に対してユニークな翻訳後修飾である蛋白質S-グアニル化(蛋白質チオール基に対するcGMP付加)をもたらす。本研究では、8-ニトロcGMPとチオール基の反応機構を解析した。また、蛋白質S-グアニル化の新しい分析法として、S-グアニル化に対する特異抗体と質量分析を組み合わせたS-グアニル化プロテオミクスを開発した。
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Report
(3 results)
Research Products
(48 results)
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[Journal Article] Mutagenicity of 8-nitroguanosine, a product of nitrative nucleoside modification by reactive nitrogen oxides, in mammalian cells2008
Author(s)
Kaneko K, Akuta T, Sawa T, Kim HW, Fujii S, Okamoto T, Nakayama H, Ohigashi H, Murakami A, and Akaike T.
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Journal Title
Cancer Lett 262
Pages: 239-47
Related Report
Peer Reviewed
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[Journal Article] Angiopoietin-related growth factor enhances blood flow via activation of the ERK1/2-eNOS-NO pathway in a mouse hind-limb ischemia model2008
Author(s)
Urano T, Ito Y, Akao M, Sawa T, Miyata K, Tabata M, Morisada T, Hato T, Yano M, Kadomatsu T, Yasunaga K, Shibata R, Murohara T, Akaike T, Tanihara H, Suda T, Oike Y.
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Journal Title
Arterioscler. Thromb. Vasc. Biol 28
Pages: 827-34
Related Report
Peer Reviewed
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[Journal Article] Protein S-guanylation by the biological signal 8-nitroguanosine 3', 5'-cyclic monophosphate2007
Author(s)
Sawa T, Zaki MH, Okamoto T, Akuta T, Tokutomi Y, Kim-Mitsuyama S, Ihara H, Kobayashi A, Yamamoto M, Fujii S, Arimoto H, and Akaike T.
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Journal Title
Nature Chem Biol 3
Pages: 727-35
NAID
Related Report
Peer Reviewed
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