Dynamic aspect of anti -inflammatory protein on inflammation and allergic desieas

• Project/Area Number: 19590316
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Osaka City University
• Principal Investigator: SATO Eisuke Osaka City University, 大学院・医学研究科, 講師 (60211942)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 炎症 / アネキシン / 活性酸素 / 抗炎症タンパク質 / アネキシン1 / Two Hybrid / KLH5 / HL-60 / トランスジェニックマウス / 好中球 / プロスタグランジン / マクロファージ / 腹腔

Research Abstract

本研究では、抗炎症タンパク質であるアネキシン1の細胞内外での作用機構を明らかにした。アネキシン1のN 末端のペプチドを2 種類合成し、本ペプチドの抗炎症作用を好中球の活性酸素産生抑制能を指標として解析すると、100μM 以上の高濃度で抑制されたが、低濃度では効果なかった。アネキシン1のトランスジェニックマウスの作製し、炎症病態を軽減することを明らかにした。また、Annexin1と結合する新規Kelch-Related Protein であるKLHL5 の他3 つの蛋白質を検出した。

Research Products

• [Journal Article] A simultaneous release of SOD1 with cytochrome c regulates mitochondria-dependent apoptosis (2009)
  • Author(s): Q Li, E F. Sato, X Zhu, and M Inoue
  • Journal Title: Mol Cell Biochem 322(1-2) Pages: 151-159
  • Peer Reviewed
• [Journal Article] Effect of obstructive jaundice and nitric oxide on the profiles of intestinal bacterial flora in wild and iNOS -/- mice (2009)
  • Author(s): Hong, EF Sato, T Nishikawa T Hiramoto K, and M Inoue
  • Journal Title: J. Clin Biochem & Nut 44(2) Pages: 160-167
  • Peer Reviewed
• [Journal Article] Acetyl-L-carnitine suppresses apoptosis of thioredoxin 2-deficient DT40 cells (2008)
  • Author(s): Xiaoping Zhu, EF. Sato, Yi Wang, H Nakamura J Yodoi, M Inoue
  • Journal Title: Arch Biochem and Biophysics. 478(2) Pages: 154-60
  • Peer Reviewed
• [Journal Article] Alpha-Lipoic acid suppresses 6-hydroxydopamin-induced ROS generation and apopotosis through the stimulation of Nrf2/glutathione pathway but not by expression of heme oxygenase-1 (2008)
  • Author(s): Fujita H, Shiosaka M, Ogino T, Okimura Y, Utsumi T, Sato EF, Akagi R, Inoue M, Utsumi K, Sasaki J
  • Journal Title: Brain Res 1206 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Effect of Fermented Papaya Preparation on Dermal and Intestinal Mucosal Immunity and Allergic Inflammations (2008)
  • Author(s): K. Hiramoto, M. Imao, E F Sato, M Inoue and A Mori
  • Journal Title: J. Sci. Food Agri 88(7) Pages: 1151-1157
  • Peer Reviewed
• [Journal Article] Fasting Differentially Modulates the Immunological System : Its Mechanism and Sex Difference (2008)
  • Author(s): K Hiramoto, T Homma, M Jikumaru, H Miyashita, E F Sato, and M Inoue
  • Journal Title: J. Clin Biochem & Nut. 43(2) Pages: 75-81
  • Peer Reviewed
• [Journal Article] Dynamic aspect of reactive oxygen and nitric oxide inoral cavity (2008)
  • Author(s): Sato EF, Choudhury T, Nishikawa T, Inoue M.
  • Journal Title: J Clin Biochem Nutr. 42 Pages: 8-13
  • Peer Reviewed
• [Journal Article] Open abdominal surgery increases intra operative oxidative stress : can it be prevented? (2008)
  • Author(s): M Tsuchiya, EF. Sato, M Inoue, and A Asada
  • Journal Title: Anesthesia&Analgesia 107(6) Pages: 1946-1952
  • Peer Reviewed
• [Journal Article] Acetyl-L-carnitine suppresses apoptosis of thioredoxin 2-deficient DT40 cells (2008)
  • Author(s): Xiaoping Zhu, EF. Sato, Yi Wang, H Nakamura J Yodoi, M Inoue
  • Journal Title: Archives of Biochemistry and Biophysics. 478 Pages: 154-160
  • Peer Reviewed
• [Journal Article] Fasting Differentially Modulates the Immunological System : Its Mechanism and Sex Difference (2008)
  • Author(s): K Hiramoto, T Homma, M Jikumaru, H Miyashita, EF Sato, M Inoue
  • Journal Title: J. Clin Biochem & Nut 43 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Dynamic aspect of reactive oxygen and nitric oxide in oral cavity. (2008)
  • Author(s): Sato EF, Choudhury T, Nishikawa T, Inoue M.
  • Journal Title: J Clin Biochem Nutr. 42 Pages: 8-13
  • Peer Reviewed
• [Journal Article] Nitrite reductase in Streptoccocus mutans plays a critical role in the survival of this pathogen in oral cavity (2007)
  • Author(s): T Choudhury, E F. Sato, M Inoue
  • Journal Title: Oral Microbiology&Immunology 22 Pages: 384-389
  • Peer Reviewed
• [Journal Article] Acupuncture enhances generation of nitric oxide and increases local circulation (2007)
  • Author(s): M Tsuchiya, EF. Sato, M Inoue, A Asada
  • Journal Title: Anesthesia and Analgesia 104(2) Pages: 301-307
  • Peer Reviewed
• [Journal Article] Potentiating effect of an endocrine disruptor, para-Nonylphenol, on the generation of reactive oxygen species (ROS) in human venous blood -association with the activation of signal transduction pathway (2007)
  • Author(s): Okai Y, Sato EF, Higashi-Okai K, Inoue M.
  • Journal Title: JUOEH 29(3) Pages: 221-233
  • NAID: 110006381791
  • Peer Reviewed
• [Journal Article] Effect of starvation on the survival of male and female mice (2007)
  • Author(s): M Jikumaru, K Hiramoto, T Honma, E F. Sato, and M Inoue
  • Journal Title: Physiol. Chem. hys. &Med. NMR 39 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Nitrite reductase in Streptoccocus mutans plays a critical role in the survival of this pathogen in oral cavity. (2007)
  • Author(s): Choudhury T, Sato EF, Inoue M.
  • Journal Title: Oral Microbiol Immunol. 22(6) Pages: 384-389
  • Peer Reviewed
• [Journal Article] Potentiating effect of an endocrine disruptor,paranonylphenol.on the generation of reactive oxygen species(ROS)in human venous blood-association with the activation of signal transduction pathway. (2007)
  • Author(s): Okai Y, Sato EF, Higashi-Okai K, Inoue M.
  • Journal Title: J UOEH. 29(3) Pages: 221-233
  • Peer Reviewed
• [Presentation] Over-expression of annexin 1 inhibits LPSInduced-inflammatory reactions in mice (2008)
  • Author(s): Zhu X, Sato EF, Kasahara E, Inoue M
  • Organizer: 第14回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Annexin1の細胞内結合タンパク質の同定とその機能解析 (2008)
  • Author(s): 佐藤英介, 笠原恵美子、内海耕造、井上正康
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Over-expression of annexin 1 inhibits LPS-induced inflammatory reactions in mice (2008)
  • Author(s): Sato EF, Zhu X, Kasahara E, Inoue M
  • Organizer: 第14回国際フリーラジカル学会
  • Place of Presentation: 北京
  • Year and Date: 2008-11-18
• [Presentation] Over-expression of annexin 1 inhibits LPS-induced inflammatory reactions in mice (2008)
  • Author(s): Sato EF, Zhu X, Kasahara E, Inoue M
  • Organizer: 第14回国際フリーラジカル学会
  • Place of Presentation: 中国・北京
  • Year and Date: 2008-11-18
• [Presentation] Annexin1の炎症場様態での機能解析 (2007)
  • Author(s): 佐藤英介、内海耕造、井上正康
  • Organizer: 第80回日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] アネキシン1の抗炎症作用機構の解析 (2007)
  • Author(s): 佐藤 英介、朱 暁萍、内海 耕慥、井上 正康
  • Organizer: 日本生化学会、分子生物学会合同学会
  • Place of Presentation: 横浜市
• [Book] ストレスの科学と健康3.8老化 (2007)
  • Author(s): 佐藤 英介、井上 正康
  • Total Pages: 319
  • Publisher: 共立出版