Functional proteomics of disease-related proteins by simultaneous and multicolor detection of posttranslational modifications of the proteins

• Project/Area Number: 19590320
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Kitasato University
• Principal Investigator: OH-ISHI Masamichi Kitasato University, 理学部, 講師 (40233027)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 蛋白質 / 糖尿病 / プロテオーム / 翻訳後修飾 / 量子ドット / 二次元電気泳動法 / ウェスタンブロッティング法

Research Abstract

二次元電気泳動法とウェスタンブロッティング法、および量子ドット法を組み合わせて、複数の蛋白質翻訳後修飾を同時に検出する方法を開発し、蛋白発現量だけではわからなかった蛋白質の翻訳後修飾異常が糖尿病発症以前から生じていることが明らかになった。

Research Products

• [Journal Article] Identification of novel immunohistochemical tumor markers for primary hepatocellular carcinoma; clathrin heavy chain and formiminotransferase cyclodeaminase (2008)
  • Author(s): Seimiya M, Tomonaga T, Matsushita K, Sunaga M, Oh-Ishi M, Kodera Y, Maeda T, Takanao S, Togawa A, Yoshitomi H, Otsuka M, Yamamoto M, Nakano M, Miyazaki M, Nomura F
  • Journal Title: Hepatology 48 Pages: 519-530
  • Peer Reviewed
• [Journal Article] Searching for new biomarkers of bladder cancer based on proteomic analysis (2008)
  • Author(s): Okusa H, Kodera Y, Oh-Ishi M, Minamida Y, Tsuchida M, Kavoussi N, Matsumoto K, Sato T, Iwamura M, Maeda T, Baba S
  • Journal Title: Journal of Electrophoresis 52 Pages: 19-24
  • Peer Reviewed
• [Journal Article] Protein carbonyl detection by two-dimensional fluorescence difference gel electrophoresis (2008)
  • Author(s): Sawada A, Ueno T, Kawashima Y, Haruta-Satoh E, Oh-Ishi M, Kodera Y, Maeda T
  • Journal Title: Journal of Electrophoresis 52 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Disease proteomics of endocrine disorders revealed by two-dimensional gel electrophoresis and mass spectrometry (2008)
  • Author(s): Oh-Ishi M, Kodera Y, Furudate S, Maeda T
  • Journal Title: Proteomics Clinical Applications 2 Pages: 327-337
  • Peer Reviewed
• [Journal Article] Identification of novel immunohistochemical tumor markers for primary hepatocellular carcinoma ; clathrin heavy chain and formi minotransferase cyclodeaminase (2008)
  • Author(s): Seimiya M, Tomonaga T, Matsushita K, Sunaga M, 0h-Ishi M. Kodera Y, Maeda T, Takanao S, Togawa A, Yoshitomi H, Otsuka M, Yamamoto M, Nakano M, Miyazaki M, Nomura F.
  • Journal Title: Hepatology 48 Pages: 519-530
  • Peer Reviewed
• [Journal Article] Disease proteomics of endocrine disorders revealed by two-dimensional gel electrophoresis and mass spectrometry. (2008)
  • Author(s): Oh-Ishi M, Kodera Y, Furudate S, Maeda T.
  • Journal Title: Proteomics Clinical Applications 2 Pages: 327-337
  • Peer Reviewed
• [Journal Article] Protein carbonyl detection by two-dimensional fluorescence difference gel electrophoresis. (2008)
  • Author(s): Sawada A, Ueno T, Kawashima Y, Haruta-Satoh E, Oh-Ishi M, Kodera Y, Maeda T.
  • Journal Title: Journal of Electrophoresis 52 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Searching for new biomarkers of bladder cancer based on proteomic analysis (2008)
  • Author(s): Hiroshi Okusa, Yoshio Kodera, Masamichi Oh-Ishi, Yutaka Minamida, Mayumi Tsuchida, Nicholas Kavoussi, Kazumasa Matsumoto, Takefumi Sato, Masatsugu Iwamura, Tadakazu Maeda and Shiro Baba
  • Journal Title: Journal of Electrophoresis 52 Pages: 19-24
  • Peer Reviewed
• [Journal Article] Detection of biomarkers for alcoholism by two-dimensional differential gel electrophoresis (2007)
  • Author(s): Wu D, Tomonaga T, Sogawa K, Satoh M, Sunaga M, Nezu M, Oh-Ishi M, Kodera Y, Maeda T, Ochiai T, Nomura F
  • Journal Title: Alcohol Clinical Experimental Research 31
  • Peer Reviewed
• [Journal Article] Disease proteomics of high-molecular-mass proteins by two-dimensional gel electrophoresis with agarose gels in the first dimension(Agarose 2-DE) (2007)
  • Author(s): Oh-Ishi M, Maeda T
  • Journal Title: Journal of Chromatography B 849 Pages: 211-222
  • Peer Reviewed
• [Journal Article] Disease proteomics of high-molecular-mass proteins by two-dimensional gel electrophoresis with agarose gels in the first dimension(Agarose 2-DE) (2007)
  • Author(s): Oh-IshiM, Maeda T.
  • Journal Title: Journal of Chromatography B 849 Pages: 211-222
  • Peer Reviewed
• [Journal Article] Detection of biomarkers for alcoholism by two-dimensional differential gel electrophoresis. (2007)
  • Author(s): Wu D, Tomonaga T, Sogawa K, Satoh M, Sunaga M, Nezu M, Oh-Ishi M, Kodera Y, Maeda T, Ochiai T, Nomura F
  • Journal Title: Alcohol Clinical Experimental Research 31
  • Peer Reviewed
• [Presentation] 牛肉偽装問題へのプロテオーム解析によるアプローチ (2008)
  • Author(s): 大石正道, 平田直之, 前田忠計
  • Organizer: 第59回日本電気泳動学会総会
  • Place of Presentation: 相模原市
  • Year and Date: 2008-11-16
• [Presentation] 牛肉偽装問題へのプロテオーム解析によるアプローチ (2008)
  • Author(s): 大石正道, 平田直之, 前田忠計
  • Organizer: 第59回日本電気泳動学会総会
  • Place of Presentation: 麻布大学(相模原市)
  • Year and Date: 2008-11-14
• [Presentation] アガロース二次元電気泳動法により発見された新規膀胱癌腫瘍マーカー (2008)
  • Author(s): 大草洋, 松本和将, 小寺義男, 土田繭美, 南田諭, 大石正道, 前田忠計, 馬場志郎
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] 原発性肝細胞癌の新たな免疫組織化学マーカー : CHCおよびFTCD (2008)
  • Author(s): 清宮正徳, 朝長毅, 松下一之, 大石正道, 小寺義男, 前田忠計, 高野重紹, 吉冨秀幸, 大塚将之, 山本雅一, 宮崎勝, 野村文夫
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Multicolor detection of posttranslational modifications of proteins on a single 2-DE pattern by quantum dot technology (2008)
  • Author(s): Oh-Ishi M, Kodera Y, Maeda T
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis(MPSA2008 : 第17回タンパク質構造解析法会議)
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-28
• [Presentation] Multicolor detection of posttranslational modifications of proteins on a single 2-DE pattern by quantum dot technology (2008)
  • Author(s): Masamichi Oh-Ishi, Yoshio Kodera, Tadakazu Maeda.
  • Organizer: 17th Meeting of Methods in Protein structure Analysis
  • Place of Presentation: 北海道大学学術交流会館(札幌)
  • Year and Date: 2008-08-26
• [Presentation] 各種プロテオーム解析技術を組み合わせた原発性肝癌のバイオマーカー探索 (2008)
  • Author(s): 野村文夫, 朝長毅, 清宮正徳, 木村明佐子, 曽川一幸, 大石正道, 小寺義男, 前田忠計
  • Organizer: 日本ヒトプロテオーム機構(JHUPO)第6回大会
  • Place of Presentation: 大阪府茨木市
  • Year and Date: 2008-07-30
• [Presentation] 量子ドット技術を用いた複数のタンパク質翻訳後修飾の同時検出 (2008)
  • Author(s): 大石正道, 小寺義男, 前田忠計
  • Organizer: 日本ヒトプロテオーム機構(JHUPO)第6回大会
  • Place of Presentation: 大阪府茨木市
  • Year and Date: 2008-07-29
• [Presentation] Simultaneous Detection of Posttranslational Modifications of Proteins by Quantum Dot Technology (2008)
  • Author(s): Masamichi Oh-Ishi, Yoshio Kodera, Tadakazu Maeda
  • Organizer: 日本ヒトプロテオーム機構第6回大会
  • Place of Presentation: ホテル阪急(大阪府吹田市)
  • Year and Date: 2008-07-28
• [Presentation] タンパク質翻訳後修飾の網羅的解析法による疾患プロテオーム解析 (2008)
  • Author(s): 大石正道
  • Organizer: 第58回日本電気泳動学会シンポジウム「基礎研究から様々な応用研究へ飛躍する最先端プロテオミクス」
  • Place of Presentation: 東京
  • Year and Date: 2008-06-13
• [Presentation] タンパク質翻訳後修飾の網羅的解析法による疾患プロテオーム解析 (2008)
  • Author(s): 大石正道
  • Organizer: 第58回日本電気泳動学会シンポジウム
  • Place of Presentation: 北里大学薬学部(東京都港区)
  • Year and Date: 2008-06-13
• [Presentation] Disease proteomics of highmolecular- mass proteins(>100kDa)by agarose 2-DE and LC-MS/MS (2008)
  • Author(s): Oh-Ishi M, Kodera Y, Maeda T, Tomonaga T, Nomura F
  • Organizer: PepCon-2008(タンパク質・アミノ酸第1回国際会議)
  • Place of Presentation: 中国・深〓
  • Year and Date: 2008-04-22
• [Presentation] Disease proteomics of high-molecular-mass proteins(>100kDa) by agarose 2-DE and LC-MS/MS (2008)
  • Author(s): Oh-Ishi M. Kodera Y, Maeda T, Tomonaga T, Nomura F.
  • Organizer: 2008 Protein and peptide conference(PepCon-2008)
  • Place of Presentation: Shenzhen, China
  • Year and Date: 2008-04-22
• [Presentation] 食肉偽装検出用ツール開発のためのプロテオーム解析2SY03シンポジウム : プロテオミクスから見た食と健康 (2008)
  • Author(s): 大石正道, 平田直之, 前田忠計
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-03-28
• [Presentation] 一次元目にアガロースIEFゲルを用いた二次元電気泳動(アガロース2-DE)法を基盤とした疾患プロテオーム解析 (2007)
  • Author(s): 大石正道
  • Place of Presentation: 山口県宇部市
  • Year and Date: 2007-11-08
• [Presentation] 一次元目にアガロースIEFゲルを用いた二次元電気泳動(アガロース2-DE)法を基盤とした疾患プロテオーム解析 (2007)
  • Author(s): 大石 正道
  • Organizer: 第58回日本電気泳動学会総会
  • Place of Presentation: 山口県宇部市
  • Year and Date: 2007-11-08
• [Presentation] Highmolecular-mass proteomics of human renal cell carcinoma by 2-DE using agarose gels for IEF (2007)
  • Author(s): Oh-Ishi M, Nii Y, Kodera Y, Okusa H, Fujita T, Iwamura M, Baba S, Maeda T
  • Organizer: HUPO 6th Annual World Congress, Seoul
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2007-10-09
• [Presentation] High-molecular-mass proteomics of human renal cell carcinoma by 2-DE using agarose gels for IEF. (2007)
  • Author(s): Oh-Ishi M, Nii Y, Kodera Y, Okusa H, Fujita T, Iwamura M, Baba S, Maeda T.
  • Organizer: HUPO 6th Annual World Congress, Seoul2007.
  • Place of Presentation: Seoul,Korea
  • Year and Date: 2007-10-09
• [Presentation] Disease proteomics of high-molecular-mass proteins by 2-DE with agarose gels in the first dimension(Agarose 2-DE)and LC-MS/MS (2007)
  • Author(s): Oh-Ishi M, Kodera Y, Maeda T
  • Organizer: 10th International Congress on Amino Acids and Proteins
  • Place of Presentation: Kallithea, Chalkidiki, Greece
  • Year and Date: 2007-08-23
• [Presentation] Disease proteomics of high-molecular-mass proteins by 2-DE with agarose gels in the first dimension(Agarose2-DE)and LC-MS/MS. (2007)
  • Author(s): Oh-Ishi M, Kodera Y, Maeda T
  • Organizer: 10th International Congress on Amino Acids and Proteins
  • Place of Presentation: Kallithea,Chalkidiki,Greece
  • Year and Date: 2007-08-23
• [Presentation] アガロース2-DEを用いたヒト腎細胞癌プロテオミクスにおけるサンプル調製法改良の重要性 (2007)
  • Author(s): 大石正道
  • Organizer: 第3回日本臨床プロテオーム研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-04-28
• [Book] 量子ドットの生命科学領域への応用 (2007)
  • Author(s): 大石正道
  • Total Pages: 248
  • Publisher: シーエムシー出版
• [Book] 量子ドットの生命科学領域への応用 (2007)
  • Author(s): 大石 正道
  • Total Pages: 248
  • Publisher: シーエムシー出版