Establishment of the diagnostic method using antibody against phosphorylated EGFR for responsiveness to gefitinib therapy of lung cancer patients

• Project/Area Number: 19590354
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Oita University
• Principal Investigator: HIJIYA Naoki Oita University, 医学部, 助教 (80305036)
• Co-Investigator(Kenkyū-buntansha): 守山 正胤 大分大学, 医学部, 教授 (90239707)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: EGFR / リン酸化 / 肺癌 / Gefitinib / 免疫組織化学 / リン酸化抗体 / 感受性診断法

Research Abstract

肺癌に対する抗癌剤であるGefitinibは、よく効く症例がある反面、まったくの無効例や重篤な副作用を発症する症例も多く、その奏効予測因子の同定が求められている。我々はEGFRのリン酸化がGefitinib感受性と高度に相関することを見出した。この知見に基づき、Gefitinib投与前にその奏功性をより簡便、迅速かつ正確に予測する診断法を確立した。

Research Products

• [Journal Article] Evaluation of Rapid Urine Test for the Detection of Helicobacter pylori Infection in the Vietnamese Population. (2009)
  • Author(s): Nguyen LT, Uchida T, Tsukamoto Y, Trinh TD, Ta L, Ho DQ, Matsuhisa T, Uchida M, Takayama A, Hijiya N, Okimoto T, Kodama M, Murakami K, Fuiioka T, Moriyama M.
  • Journal Title: Digestive diseases and sciences
  • Peer Reviewed
• [Journal Article] Phosphorylation status of epidermal growth factor receptor is closely associated with responsiveness to gefitinib in pulmonary adenocarcinoma (2008)
  • Author(s): Hijiya N., Miyawaki M., Kawahara K., Akamine S., Tsuji K., Kadota J., Akizuki S., Uchida T., Matsuura K., Tsukamoto Y., Moriyama M.
  • Journal Title: Hum Pathol 39 Pages: 316-323
  • Peer Reviewed
• [Journal Article] Arpp/Ankrd2, a member of the muscle ankyrin repeat proteins (MARPs), translocates from the I-band to the nucleus after muscle injury (2008)
  • Author(s): Tsukamoto Y., Hijiya N., Yano S., Yokoyama S., Nakada C., Uchida T., Matsuura K., Moriyama M.
  • Journal Title: Histochem Cell Biol 129 Pages: 55-64
  • Peer Reviewed
• [Journal Article] Genotyping of the cagA gene of Helicobacter pylori on immunohistochemistry with East Asian CagA-specific antibody (2008)
  • Author(s): Kanada R., Uchida T., Tsukamoto Y., Nguyen LT., Hijiya N., Matsuura K., Kodama M., Okimoto T., Murakami K., Fujioka T., Yanagisawa S., Moriyama M.
  • Journal Title: Pathol Int 58 Pages: 218-225
  • NAID: 10027376254
  • Peer Reviewed
• [Journal Article] Genome-wide microRNA expression profiling in renal cell carcinoma : significant downregulation of miR-141 and miR-200c (2008)
  • Author(s): Nakada C., Matsuura K., Tsukamoto Y., Tanigawa M., Yoshimoto T., Narimatsu T., Nguyen LT., Hijiya N., Uchida T., Sato F., Mimata H., Seto M., Moriyama M.
  • Journal Title: J Pathol 216 Pages: 418-427
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of DNA copy number alterations and gene expression in gastric cancer (2008)
  • Author(s): Tsukamoto Y., Uchida T., Karnan S., Noguchi T., Nguyen LT., Tanigawa M., Takeuchi I., Matsuura K., Hijiya N., Nakada C., Kishida T., Kawahara K., Ito H., Murakami K., Fujioka T., Seto M., Moriyama M.
  • Journal Title: J Pathol 216 Pages: 471-482
  • Peer Reviewed
• [Journal Article] Enhanced phosphorylation of the epidermal growth factor receptor at the site of tyrosine 992 in esophageal carcinomas (2008)
  • Author(s): Miyawaki M., Hijiya N., Tsukamoto Y., Nakada C., Kawahara K., Moriyama M.
  • Journal Title: APMIS 116 Pages: 1097-1106
  • Peer Reviewed
• [Journal Article] Enhanced phosphorylation of the epidermal growth factor receptor at the site oftyrosine992 in esophageal carcinomas. (2008)
  • Author(s): Miyawaki M, Hijiya N, Tsukamoto Y, Nakada C, Kawahara K, Moriyama M.
  • Journal Title: APMIS 116 Pages: 1097-1106
  • Peer Reviewed
• [Journal Article] Genome-wide microRNA expression profiling in renal cell carcinoma : significant down-regulation of miR-141 and miR-200c. (2008)
  • Author(s): Nakada C, Matsuura K, Tsukamoto Y, Tanigawa M, Yoshimoto T, Narimatsu T, Nguyen LT, Hiiiya N, Uchida T, Sato F, Mimata H, Seto M, Moriyama M.
  • Journal Title: The Journal of pathology 216 Pages: 418-427
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of DNA copy number alterations and gene expression in gastric cancer. (2008)
  • Author(s): Tsukamoto Y, Uchida T, Kaman S, Noguchi T, Nguyen LT, Tanigawa M, Takeuchi I, Matsuura K, Hijiya N, Nakada C, Kishida T, Kawahara K, Ito H, Murakami K, Fujioka T, Seto M, Moriyama M.
  • Journal Title: The Journal of pathology 216 Pages: 471-82
  • Peer Reviewed
• [Journal Article] Genotyping of the cagA gene of Helicobacter pylori on immunohistochemistry with East Asian CagA-specific antibody. (2008)
  • Author(s): Kanada R, Uchida T, Tsukamoto Y, Nguyen LT, Hijiya N. Matsuura K, Kodama M, Okimoto T, Murakami K, Fujioka T, Yanagisawa S, Moriyama M.
  • Journal Title: Pathology International 58 Pages: 218-225
  • NAID: 10027376254
  • Peer Reviewed
• [Journal Article] Phosphorylations status of epidermal growth factor receptor is closely associated with responsiveness to gefitinib in pulmonary adenocarcinoma. (2008)
  • Author(s): Hijiya N.
  • Journal Title: Human Pathology 39 Pages: 316-323
  • Peer Reviewed
• [Journal Article] Arpp/Ankrd2, a member of the muscle ankyrin repeat proteins(MARPs),translocates from the I-band to the nucleus after muscle injury. (2008)
  • Author(s): Tsukamoto Y.
  • Journal Title: Histochemistry and cell biology 129 Pages: 55-64
  • Peer Reviewed
• [Journal Article] Upregulated expression of cardiac ankyrin-repeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis (2007)
  • Author(s): Matsuura K., Uesugi N., Hijiya N., Uchida T., Moriyama M.
  • Journal Title: Hum Pathol 38 Pages: 410-419
  • Peer Reviewed
• [Journal Article] ARPP protein is selectively expressed in renal oncocytoma, but rarely in renal cell carcinomas (2007)
  • Author(s): Shomori K, Nagashima Y, Kuroda N, Honjo A, Tsukamoto Y., Tokuyasu N., Maeta N., Matsuura K., Hijiya N., Yano S., Yokoyama S., Ito H., Moriyama M.
  • Journal Title: Mod Pathol 20 Pages: 199-207
  • Peer Reviewed
• [Journal Article] Immunohistochemical diagnosis of the cagA-gene genotype of Helicobacter pylori with anti-East Asian CagA-specific antibody (2007)
  • Author(s): Uchida T., Kanada R., Tsukamoto Y., Hijiya N., Matsuura K., Yano S., Yokoyama S., Kishida T., Kodama M., Murakami K., Fujioka T., Moriyama M.
  • Journal Title: Cancer Sci 98 Pages: 521-528
  • Peer Reviewed
• [Journal Article] Aortic root subadventitial hematoma (2007)
  • Author(s): Miyamoto S., Hadama T., Iwata E., Anai H., Sako H., Wada T., Tanaka H., Hamamoto H., Hijiya N.
  • Journal Title: Heart Vessels 22 Pages: 136-138
  • Peer Reviewed
• [Journal Article] Immunohistochemical diagnosis of the cagA-gene genotype of Helicobacter pylori with anti-East Asian CagA-specific antibody. (2007)
  • Author(s): Uchida T.
  • Journal Title: Cancer Science 98 Pages: 521-528
  • Peer Reviewed
• [Presentation] 腎癌におけるmiRNAの網羅的発現解析(Genome-wide microRNA expression profiling in renal cell carcinoma) (2008)
  • Author(s): 中田知里, 松浦恵子, 塚本善之, 谷川雅人, 吉本多一郎, 泥谷直樹, 内田智久, 三股浩光, 瀬戸加大, 守山正胤
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 胃癌におけるゲノムコピー数異常と遺伝子発現異常の統合解析(Genome-wide analysis of DNA copy number alterations and gene expression in gastric cancer) (2008)
  • Author(s): 塚本善之, 内田智久, カルナン・シバスンダラム, 野口剛, 谷川雅人, 竹内一郎, 松浦恵子, 中田知里, TungNguyen, 泥谷直樹, 井藤久雄, 瀬戸加大, 守山正胤
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Phosphorylation status of EGFR is closely associated with responsiveness to gefitinib in pulmonary adenocarcinoma (2007)
  • Author(s): 泥谷直樹
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
• [Presentation] 抗リン酸化EGFR抗体を用いた肺腺癌のGefitinib感受性予測診断法の確立 (2007)
  • Author(s): 泥谷直樹
  • Organizer: 第96回日本病理学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Phosphorylation status of EGFR is closely associated with responsiveness to gefitinib in Pulmonary adenocarcinoma (2007)
  • Author(s): 泥谷直樹
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/daigakuin/fandamental/indexO2.html
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/daigakuin/fandamental/index02.html