Study on genetic analyses for pathological diagnosis and therapy in glioma

• Project/Area Number: 19590360
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Nara Medical University
• Principal Investigator: MITSUTOSI Nakamura Nara Medical University, 医学部, 准教授 (00305715)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: glioblastoma / glioma / heterogeneity / chemotherapy / O^6-MGMT / temozolomide / cancer stem cell

Research Abstract

組織多様性を示す神経膠腫では各組織型におけるgenotype が異なる場合があり、治療を含めた臨床病理学的な診断には、遺伝子変異の解析が必要な場合がある。特に悪性神経膠腫においては治療感受性を判定するには、組織型ごとにO6-MGMTをはじめ複数の因子を解析することによって最適の治療を選択できると考えられた。

Research Products

• [Journal Article] A novel human AlkB homologue, ALKBH8, contributes to human bladder cancer progression (2009)
  • Author(s): Shimada K, Nakamura M, Konishi N(他5名)
  • Journal Title: Cancer Res 69 Pages: 3157-3164
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of neuroendocrine differentiation in prostate cancer (2009)
  • Author(s): Ishida E, Nakamura M. Konishi N (他2名)
  • Journal Title: Pathobiology 76 Pages: 30-38
  • Peer Reviewed
• [Journal Article] Isoflurane exerts a short-term but not a long-term preconditioning effect in neonatal rats exposed to a hypoxic-ischaemic neuronal injury (2009)
  • Author(s): Sasaoka N, Nakamura M , Konishi N (他2名)
  • Journal Title: Acta Anaesthesiol Scand 53 Pages: 46-54
• [Journal Article] Function of JunB in transient amplifying cell senescence and progression of human prostate cancer (2008)
  • Author(s): Konishi N, Nakamura M (他5名)
  • Journal Title: Clin Cancer Res 14(14) Pages: 4408-16
  • Peer Reviewed
• [Journal Article] Prostate cancer antigen-1 contributes to cell survival and invasion though discoidin receptor 1 in human prostate cancer (2008)
  • Author(s): Shimada K, Nakamura M (他4名), Konishi N
  • Journal Title: Cancer Sci 99 Pages: 39-45
  • Peer Reviewed
• [Journal Article] Cytologic feature by squash preparation of pineal parenchyma tumor of intermediate differentiation (2008)
  • Author(s): Shimada K, Nakamura M (他4名)
  • Journal Title: Diagn Cytopathol 36 Pages: 749-53
  • Peer Reviewed
• [Journal Article] HRK inactivation associated with promoter methylation and LOH in prostate cancer (2008)
  • Author(s): Higuchi T, Nakamura M(他4名)
  • Journal Title: Prostate, 68 Pages: 105-13
  • Peer Reviewed
• [Journal Article] Effects of delta-opioid agonist SNC80 on white matter injury following spinal cord ischemia in normothermic and mildly hypothermic rats (2008)
  • Author(s): Horiuchi, T, Nakamura M (他5名), Konishi N
  • Journal Title: J Anesth 22 Pages: 32-7
  • Peer Reviewed
• [Journal Article] グリオーマ治療観点からの病理診断の意義 (2008)
  • Author(s): 中村光利, 小西登
  • Journal Title: 病理と臨床 26 Pages: 86-7
• [Journal Article] Prostate cancer antigen-1 contributes to cell survival and invasion though discoidin receptor 1 in human prostate cancer. (2008)
  • Author(s): Shimada, K., Nakamura, Konishi, N., et al
  • Journal Title: Cancer Sci 99 Pages: 39-45
  • Peer Reviewed
• [Journal Article] Effects of delta-opioid agonist SNC80 on white matter injury following spinal cord ischemia in normothermic and mildly hypothermic rats. (2008)
  • Author(s): Horiuchi, T., Nakamura, Konishi, N., et al
  • Journal Title: J Anesth 22 Pages: 32-37
  • Peer Reviewed
• [Journal Article] Function of JunB in transient amplifying cell senescence and progression of human prostate cancer. (2008)
  • Author(s): Konishi, N. Nakamura, et al
  • Journal Title: Clin Cancer Res 14 Pages: 4408-4416
  • Peer Reviewed
• [Journal Article] Cytologic feature by squash preparation of pineal parenchyma tumor of intermediate differentiation. (2008)
  • Author(s): Shimada, K., Nakamura, Konishi, N., et al
  • Journal Title: Diagn Cytopathol 36 Pages: 749-753
  • Peer Reviewed
• [Journal Article] Isoflurane exerts a short-term but not a long-term preconditioning effect in neonatal rats exposed to a hypoxic-ischaemic neuronalinjury. (2008)
  • Author(s): Sasaoka, N., Nakamura, Konishi, N., et al
  • Journal Title: Acta Anaesthesiol Scand 53 Pages: 46-54
  • Peer Reviewed
• [Journal Article] グリオーマ治療観点からの病理診断の意義 (2008)
  • Author(s): 中村 光利, 小西 登
  • Journal Title: 病理と臨床 26 Pages: 86-87
• [Journal Article] HRK inactivation associated with promoter methylation and LOH in prostate cancer (2008)
  • Author(s): Higuchi, T., Nakamura, M. Konishi, N., et. al.
  • Journal Title: Prostate 68 Pages: 105-113
  • Peer Reviewed
• [Journal Article] 脳腫瘍の病理診断における遺伝子解析の有用性 (2007)
  • Author(s): 中村光利, 小西登
  • Journal Title: Neuro-Oncologyの進歩 16 Pages: 7-11
  • NAID: 130001933527
  • Peer Reviewed
• [Journal Article] c-Jun NH2 terminal kinase activation and decreased expression of mitogen-activated protein kinase phosphatase-1 play important roles in invasion and angiogenesis of urothelial carcinomas (2007)
  • Author(s): Shimada K, Nakamura M, Konishi N 他4名
  • Journal Title: Am J Pathol 171_ Pages: 1003-12
  • Peer Reviewed
• [Journal Article] c-Jun NH2 terminal kinase activation and decreased expression of mitogen-activated protein kinase phosphatase-1 play important roles in invasion and angiogenesis of urothelial carcinomas (2007)
  • Author(s): Shin K, Nakamura M (他3名), Konishi N
  • Journal Title: Neurol Res 29 Pages: 727-33
  • Peer Reviewed
• [Journal Article] A rare case of malignant glioma suspected to have arisen from a cavernous sinus (2007)
  • Author(s): Taomoto, K, Nakamura M (他8名)
  • Journal Title: Brain Tumor Pathol 24 Pages: 75-80
  • Peer Reviewed
• [Journal Article] Effects of delta-opioid receptor stimulation and inhibition on hippocampal survival in a rat model of forebrain ischaemia (2007)
  • Author(s): Iwata M, Nakamura M (他3名), Konishi N
  • Journal Title: Br J Anaesth 99 Pages: 538-46
  • Peer Reviewed
• [Journal Article] Molecular pathogenesis of pediatric astrocytic tumors (2007)
  • Author(s): Nakamura, M, Shimada, K, Konishi, N., et. al.
  • Journal Title: Neuro-Oncol 9 Pages: 113-123
  • Peer Reviewed
• [Journal Article] 脳腫瘍の病理診断における遺伝子解析の有用性 (2007)
  • Author(s): 中村 光利, 小西 登, 他
  • Journal Title: Neruo-Oncologyの進歩 16 Pages: 7-11
  • NAID: 130001933527
  • Peer Reviewed
• [Journal Article] Expression of angiogenic growth factor in the rat DAVFmodel (2007)
  • Author(s): Shin, Y., Nakamura, M., Konishi, N., Sakaki, T., et. al.
  • Journal Title: Neurol Res 29 Pages: 727-733
  • Peer Reviewed
• [Journal Article] DNA hypermethylation status of multiple genes in papillarythyroid carcinomas (2007)
  • Author(s): Ishida, E., Nakamura, M., Konishi, N., et. al.
  • Journal Title: Pathobiology 74 Pages: 344-352
  • Peer Reviewed
• [Journal Article] Prostate cancer antigen-1 contributes to cell survival and invasionthough discoidin receptor l in human prostate cance (2007)
  • Author(s): Shimada, K., Nakamura, M., Konishi, N., et. al.
  • Journal Title: Cancer Sci 99 Pages: 39-45
  • Peer Reviewed
• [Journal Article] c-Jun NH2 terminal kinase activation and decreased expression of mitogen-activated protein kinase phosphatase-1 play important roles in invasion and angiogenesis of urothelial carcinomas (2007)
  • Author(s): Shimada, K., Nakamura, M., Konishi, N, et. al.
  • Journal Title: Am J Pathol 171 Pages: 1003-1012
  • Peer Reviewed
• [Journal Article] Co-transplantation of mouse embryonic stem cells and bone marrow stromal cells following spinal cord injury suppresses tumor development
  • Author(s): Matsuda R, Nakamura M (他12名)
  • Journal Title: Cell Transplant in press
  • Peer Reviewed
• [Journal Article] The role of HRK gene in human cancer
  • Author(s): Nakamura M, Shimada K, Konishi N
  • Journal Title: Oncogene in press
• [Journal Article] Genotype 解析からみたグリオーマの診断
  • Author(s): 中村光利, 小西登
  • Journal Title: BRAIN and NERVE,総説 印刷中
• [Presentation] 前立腺癌治療後の上皮細胞および間質細胞における遺伝子変異の検討 (2009)
  • Author(s): 中村光利, 島田啓司, 小西登 他1名
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-03
• [Presentation] 組織多様性を示す脳神経膠腫におけるgenotype解析の有用性 (2008)
  • Author(s): 中村光利, 小西登 他3名
  • Organizer: 第28回日本脳腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-09
• [Presentation] 組織多様性を示す悪性神経膠腫におけるTMZ感受性因子としてのO6-MGMT 遺伝子の検索 (2008)
  • Author(s): 中村光利, 小西登 他3名
  • Organizer: 第28回日本脳腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-09
• [Presentation] 組織多様性を示す悪性神経膠腫におけるtemozolomide 感受性因子の検索 (2008)
  • Author(s): 中村光利, 小西登 他3名
  • Organizer: 第28回日本脳腫瘍学会
  • Place of Presentation: 愛媛
  • Year and Date: 2008-11-30
• [Presentation] 組織多様性を示す悪性神経膠腫におけるtemozolomide感受性因子の検索 (2008)
  • Author(s): 中村光利, 小西 登, 他
  • Organizer: 第28回日本脳腫瘍学会
  • Place of Presentation: 愛媛
  • Year and Date: 2008-11-30
• [Presentation] O^6-MGMT status as a marker for response to temozolomide in malignant gliomas with histological heterogeneity (2008)
  • Author(s): 中村光利, 島田啓司, 石田英和, 小西登
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-30
• [Presentation] Human ABH-8 is a new molecule for the development of bladder cancer (2008)
  • Author(s): 島田啓司, 中村光利, 小西登 他4名
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Expression of human ABH families in non-small cell lung carcinoma (2008)
  • Author(s): 田崎正人, 中村光利, 小西登 他3名
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] DNA microarray analysis of multicentric prostate cancer based on the computer-assisted three-dimensional model (2008)
  • Author(s): 石田英和, 中村光利, 小西登 他2名
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] 組織多様性を示す悪性神経膠腫における TMZ 感受性因子としてのO6-MGMT 遺伝子の検索 (2008)
  • Author(s): 中村光利, 小西登 他4名
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] 前立腺progenitor cellの生物学的特性と病理組織学的意義について (2008)
  • Author(s): 島田啓司, 中村光利, 小西登 他2名
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] 組織多様性を示す glioblastoma における genotype 解析の有用性 (2008)
  • Author(s): 田崎正人, 中村光利, 小西登 他3名
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] 組織多様性を示す悪性神経膠腫におけるTMZ感受性因子としてのO^6-MGMT遺伝子の検索 (2008)
  • Author(s): 中村光利, 小西 登, 他
  • Organizer: 第97回日本病理会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] 組織多様性を示すglioblastomaにおけるgenotype解析の有用性 (2008)
  • Author(s): 田崎正人, 中村光利, 小西 登, 他
  • Organizer: 第97回日本病理会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] 前立腺progenitor cellの生物学的特性と病理組織学的意義について (2008)
  • Author(s): 島田啓司, 中村光利, 小西 登, 他
  • Organizer: 第97回日本病理会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] 組織多様性を示す脳神経膠腫におけるgenotype解析の有用性 (2007)
  • Author(s): 中村 光利, 島田 啓司, 石田 英和, 樋口 智紀, 小西 登
  • Organizer: 第25回日本脳腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2007-12-09
• [Presentation] 組織多様性を示す悪性神経膠腫におけるTMZ感受性因子としてのO^6-MGMT遺伝子の検索 (2007)
  • Author(s): 中村 光利, 島田 啓司, 石田 英和, 樋口 智紀, 小西 登
  • Organizer: 第25回日本脳腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2007-12-09
• [Presentation] 組織多様性を示す神経膠腫におけるテモゾロミド感受性因子としてのO6-MGMT 遺伝子メチル化検索 (2007)
  • Author(s): 中村光利, 小西登 他3名
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] 組織多様性を示す脳神経膠腫におけるgenotype 解析 (2007)
  • Author(s): 樋口智紀, 中村光利, 小西登 他2名
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] 組織多様性を示す脳神経膠腫におけるgenotype解析 (2007)
  • Author(s): 樋口 智紀, 中村 光利, 島田 啓司, 石田 英和, 小西 登
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] 組織多様性を示す神経膠腫におけるテモゾロミド感受性因子としてのO^6-MGMT遺伝子メチル化検索 (2007)
  • Author(s): 中村 光利, 島田 啓司, 石田 英和, 樋口 智紀, 小西 登
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] 前立腺癌Gleason gradeにおける分子マーカーの検討 (2007)
  • Author(s): 中村 光利, 島田 啓司, 石田 英和, 樋口 智紀, 小西 登
  • Organizer: 第18回前立腺がんワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2007-09-28
• [Presentation] 組織多様性を示す脳神経膠腫におけるgenotype 解析の有用性 (2007)
  • Author(s): 樋口智紀, 中村光利 (2番目), 小西登 他3名.
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-15
• [Presentation] Oligodendrogliomaにおける病理組織学的診断と遺伝子解析の有用性 (2007)
  • Author(s): 中村光利, 小西登 他4名
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-15