Malignant transformation of urinary bladder carcinoma cells compared by telomere length measured by original Q-FISH method

• Project/Area Number: 19590375
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Tokyo Metropolitan Institute of Gerontology
• Principal Investigator: IZUMIYAMA Naotaka Tokyo Metropolitan Institute of Gerontology, 東京都老人総合研究所, 助手 (10158751)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Ken-ichi 財団法人東京都高齢者研究・福祉振興財団, 東京都老人総合研究所, 研究員 (60159069) ; TAKUBO Kaiyo 財団法人東京都高齢者研究・福祉振興財団, 東京都老人総合研究所, 研究部長 (00154956) ; ISHII Akio 財団法人東京都高齢者研究・福祉振興財団, 東京都老人総合研究所, 助手 (60167244)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: テロメア / セントロメア / Q-FISH

Research Abstract

Diploid5例の平均テロメア長は6.7kbpであり、G3を含む悪性度の高い例でテロメアが短かった。5例に共通してテロメアが有意に短縮する染色体は存在しないが、12p(Kras遺伝子搭載)は5例中3例で有意に短縮していた。染色体不安定性の指標であるアナフェーズブリッジの出現頻度はテロメア長と逆相関を示し、テロメアの短縮が染色体不安定性に深く関与していることが明らかになった。

Research Products

• [Journal Article] Telomere shortening in Barrett's mucosa and esophageal adenocarcinoma and its association with loss of heterozygosity. (2009)
  • Author(s): Shiraishi H, Mikami T, Aida J, Nakamura K, Izumiyama-Shimomura N, Arai T, Watanabe M, Okayasu I, Takubo K.
  • Journal Title: Scand J Gastroenterol. 44(5) Pages: 538-44
  • Peer Reviewed
• [Journal Article] Telomere shortening in Barrett's mucosa and esophageal adenocarcinoma and its association with loss of heterozygosity (2009)
  • Author(s): Shiraishi H, Aida J, Nakamura K, Izumiyama-Shimomura N, Takubo K., 他4名
  • Journal Title: Scand J Gastroenterol 44(5) Pages: 533-44
  • Peer Reviewed
• [Journal Article] Luminal and cancer cells in the breast show more rapid telomere shortening than myoepithelial cells and fibroblasts. (2008)
  • Author(s): Kurabayashi R, Takubo K, Aida J, Honma N, Poon SS, Kammori M, Izumiyama-Shimomura N, Nakamura KI, Tsuji EI, Matsuura M, Ogawa T, Kaminishi M.
  • Journal Title: Hum Pathol. Pages: 0-0
  • Peer Reviewed
• [Journal Article] The teleost Oryzias latipes shows telomere shortening with age despite considerable telomerase activity throughout life. (2008)
  • Author(s): Hatakeyama H, Nakamura K, Izumiyama-Shimomura N, Ishii A, Tsuchida S, Takubo K, Ishikawa N.
  • Journal Title: Mech Ageing Dev. 129(9) Pages: 550-7
  • Peer Reviewed
• [Journal Article] Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium. (2008)
  • Author(s): Aida J, Izumiyama-Shimomura N, Nakamura K, Ishikawa N, Poon SS, Kammori M, Sawabe M, Arai T, Matsuura M, Fujiwara M, Kishimoto H, Takubo K.
  • Journal Title: Exp Gerontol. 43(9) Pages: 833-9
• [Journal Article] Luminal and cancer cells in the breast show more rapid telomere shortening than myoepithelial cells and fibroblasts (2008)
  • Author(s): Kurabayashi R, Takubo K, Aida J, Izumiyama-Shimomura N, Nakamura KI., 他7名
  • Journal Title: Hum Pathol 39 Pages: 1647-1655
  • Peer Reviewed
• [Journal Article] Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium (2008)
  • Author(s): Aida J, Izumiyama-Shimomura N, Takubo K., 他9名
  • Journal Title: Exp Gerontol 43 Pages: 833-9
  • Peer Reviewed
• [Journal Article] Squamous cell carcinomas of the esophagus arise from a telomere-shortened epithelial field. (2007)
  • Author(s): Kammori M, Poon SS, Nakamura K, Izumiyama N, Ishikawa N, Kobayashi M, Naomoto Y, Takubo K.
  • Journal Title: Int J Mol Med. 20(6) Pages: 793-9
  • Peer Reviewed
• [Journal Article] Telomeric DNA length in cerebral gray and white matter is associated with longevity in individuals aged 70 years or older. (2007)
  • Author(s): Nakamura K, Takubo K, Izumiyama-Shimomura N, Sawabe M, Arai T, Kishimoto H, Fujiwara M, Kato M, Oshimura M, Ishii A, Ishikawa T. N.
  • Journal Title: Exp Gerontol. 42(10) Pages: 944-50
  • Peer Reviewed
• [Journal Article] Telomere length variations in 6 mucosal cell types of gastric tissue observed using a novel quantitative fluorescence in situ hybridization method. (2007)
  • Author(s): Aida J, Izumiyama-Shimomura N, Nakamura K, Ishii A, Ishikawa N, Honma N, Kurabayashi R, Kammori M, Poon SS, Arai T, Takubo K.
  • Journal Title: Hum Pathol. 38(8) Pages: 1192-200
  • Peer Reviewed
• [Journal Article] Squamous cell carcinoma of the esophagus arise from a telomere-shortened epithelial field. (2007)
  • Author(s): Kammori M, Poon SS, Nakamura K, Izumiyama N, Ishikawa N, Kobayashi M, Naomoto Y, Takubo K.
  • Journal Title: Int J Mol Med 20 Pages: 793-799
  • Peer Reviewed
• [Journal Article] Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium.
  • Author(s): Aida J, Izumiyama-Shimomura N, Nakamura K, Ishikawa N, Poon SSS., 他5名
  • Journal Title: Exp Gerontol (in press)
  • Peer Reviewed
• [Journal Article] Luminal and cancer cells in the breast show more rapid telomere shortening than myoepithelial cells and fibroblasts.
  • Author(s): Kurabayashi R, Takubo K, Aida J, Honma N, Poon SSS, Kammori M, Izumiyama-Shimomura N., 他5名
  • Journal Title: Hum Pathol (in press)
  • Peer Reviewed
• [Presentation] 食道癌の背景上皮と非担癌上皮のテロメア長と染色体の不安定性 (2008)
  • Author(s): 田久保海誉
  • Organizer: 第67回日本癌学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-30
• [Presentation] 食道癌の背景上皮と非担癌上皮のテロメア長と染色体の不安定性 (2008)
  • Author(s): 田久保海誉, 他6名
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-30
• [Presentation] 組織Q-FISH法による舌癌発生母地のテロメア短縮の証明と分裂後期架橋 (2008)
  • Author(s): 相田順子
  • Organizer: 第67回日本癌学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] 組織Q-FISH法による乳癌組織構成5細胞のテロメア長測定 (2008)
  • Author(s): 倉林理恵
  • Organizer: 第67回日本癌学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] 舌上皮内腫瘍性病変のテロメア-組織Q-FISH法による構成細胞別アプローチ (2008)
  • Author(s): 相田順子
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 石川県立音楽堂
  • Year and Date: 2008-05-17
• [Presentation] 小児生体肝移植後長期経過例におけるドナーとレシピエント肝のテロメア長-tissue Q-FISH法を用いた検討- (2008)
  • Author(s): 川野陽一
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 石川県立音楽堂
  • Year and Date: 2008-05-17
• [Presentation] ヒト下垂体の加齢に伴うテロメア短縮 : 第2報 (2008)
  • Author(s): 泉山七生貴
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 石川県立音楽堂
  • Year and Date: 2008-05-16
• [Presentation] Q-FISH法による先天性疾患の責任遺伝子搭載染色体のpq腕別のテロメア長の解析 (2008)
  • Author(s): 仲村賢一
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 石川県立音楽堂
  • Year and Date: 2008-05-15
• [Presentation] 前処置を工夫した組織Q-FISH法によるヒト表皮細胞テロメア長加齢の変化 (2008)
  • Author(s): 笠原一郎
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 石川県立音楽堂
  • Year and Date: 2008-05-15
• [Presentation] 食道の扁平上皮癌はテロメア短縮領域から発生する (2007)
  • Author(s): 仲村賢一
  • Organizer: 第66回日本癌学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-05
• [Presentation] 組織Q-FISH法を用いた甲状腺乳頭癌のテロメア長解析 (2007)
  • Author(s): 柏尾光彦
  • Organizer: 第66回日本癌学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-04
• [Presentation] 大脳白質および灰白質のテロメア長は癌死率と反比例し長寿命と正相関する (2007)
  • Author(s): 石川直
  • Organizer: 第66回日本癌学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-04
• [Presentation] Telomere shortening in Barrett's mucosa and Adenocarcinoma and its significant association with loss of heterozygosity. (2007)
  • Author(s): 白石廣照
  • Organizer: 第66回日本癌学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-04
• [Presentation] 食道の扁平上皮癌はテロメア短縮領域から発生する. (2007)
  • Author(s): 仲村 賢一, 下村 七生貴, 石井 章雄, 石川 直, 田久保 海誉. 他4名
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜