Accelerated tumor growth in mice deficient in DNAM-1 receptor

• Project/Area Number: 19590382
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: University of Tsukuba
• Principal Investigator: TAHARA Satoko University of Tsukuba, 大学院・人間総合科学研究科, 助教 (20360589)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: DNAM-1 / CD226 / CD155 / CD112 / 免疫監視 / 腫瘍 / メチルコラントレン / DMBA / メチルコラントン / 化学癌誘発剤

Research Abstract

DNAM-1(CD226)は、細胞傷害性T細胞およびNK細胞などのキラーリンパ球に強く発現しており、腫瘍細胞などに発現するリガンド(CD155およびCD112)を認識して接着し腫瘍細胞に細胞傷害を誘導する。本研究では、腫瘍に対する免疫監視機構におけるDNAM-1の機能を明らかにするため、DNAM-1遺伝子欠損マウスを用いて腫瘍モデルを検討した。その結果、メチルコラントレン25μgを皮下に接種するとDNAM-1遺伝子欠損マウスでは腫瘍の発生率が亢進し、生存率が低下することを見出した。このことから、キラーリンパ球に発現するDNAM-1は、腫瘍発生に対する免疫監視機構において重要な役割を有することを明らかにした。

Research Products

• [Journal Article] Identification of the Fcα/μR isoform specifically expressed in the kidney tubule (2009)
  • Author(s): Kurita N, Tahara-Hanaoka S, et.al.
  • Journal Title: Mol Immunol 46 Pages: 749-753
  • NAID: 120007138354
  • Peer Reviewed
• [Journal Article] dentification of the Fca/μR isoform specifically expressed in the kidney tubule (2009)
  • Author(s): Kurita N et. al.
  • Journal Title: Molecular Immunology 46 Pages: 749-753
  • Peer Reviewed
• [Journal Article] Brief Definitive Report. Accelerated tumor growth in mice deficient in DNAM-1 receptor (2008)
  • Author(s): Iguchi-Manaka A, Kai H, Tahara-Hanaoka S, et.al.
  • Journal Title: J Exp Med 205 Pages: 2959-2964
  • Peer Reviewed
• [Journal Article] Activation of neutrophils by a novel triggering immunoglobulin-like receptor MAIR-IV (2008)
  • Author(s): Nakano T, Tahara-Hanaoka S, et.al.
  • Journal Title: Mol Immunol 45 Pages: 289-294
  • NAID: 130006950072
  • Peer Reviewed
• [Journal Article] Caspase-independent cell death by CD300LF (MAIR-V), an inhibitory immunoglobulin-like receptor on myeloid cells (2008)
  • Author(s): Can I, Tahara-Hanaoka S, et.al.
  • Journal Title: J Immunol 180 Pages: 207-213
  • Peer Reviewed
• [Journal Article] Expression of a splicing isoform ofx MAIR-V (CD300LF), an inhibitory immunoglobulin-like receptor on myeloid cells. (2008)
  • Author(s): Can I, Tahara-Hanaoka S, Shibuya A.
  • Journal Title: Hybridoma 27 Pages: 59-61
  • Peer Reviewed
• [Journal Article] LFA-1 decreases the antigen dose for T cell activation in vivo (2008)
  • Author(s): Wang Y, Shibuya K, Tahara-Hanaoka S, et.al.
  • Journal Title: Int Immunol 20 Pages: 1119-27
  • Peer Reviewed
• [Journal Article] Brief Definitive Report. Accelerated tumor growth in mice deficient in DNAM-1 receptor (2008)
  • Author(s): Iguchi-Manaka A et. al.
  • Journal Title: Journal of Experimental Medicine 205 Pages: 2959-2964
  • Peer Reviewed
• [Journal Article] Activation of neutrophils by a novel triggering immunoglobulin-like receptor MAIR-IV (2008)
  • Author(s): Nakano T et. al.
  • Journal Title: Molecular Immunology 45 Pages: 289-294
  • Peer Reviewed
• [Journal Article] Caspase-independent cell death by CD300LF (MAIR-V), an inhibitory immunoglobulin-like receptor on myeloid cells (2008)
  • Author(s): Can I et. al.
  • Journal Title: Journal of Immunology 180 Pages: 207-213
  • Peer Reviewed
• [Journal Article] Expression of a splicing isoform of MAIR-V (CD300LF), an inhibitory immunoelobulin-like receptor on myeloid cells (2008)
  • Author(s): Can I et. al.
  • Journal Title: Hybridoma 27 Pages: 259-261
  • Peer Reviewed
• [Journal Article] LFA-1 decreases the antigen dose for T cell activation in vivo (2008)
  • Author(s): Wang Y et. al.
  • Journal Title: International Immunology 20 Pages: 1119-1127
  • Peer Reviewed
• [Journal Article] Expression of a Splicing Isoform of MAIR-V(CD300LF), an inhibitory immunoglobulin-like receptor on myeloid cells. (2008)
  • Author(s): Can I, et. al.
  • Journal Title: Hybridoma 27:1 Pages: 59-61
  • Peer Reviewed
• [Journal Article] Caspase-independent cell death by CD300LF(MAIR-V), an inhibitory immunoglobulin-like receptor on myeloid cells (2008)
  • Author(s): Can I, et. al.
  • Journal Title: Journal of Immunology 180:1 Pages: 207-13
  • Peer Reviewed
• [Journal Article] Dual assemblies of an activating immune receptor, MAIR-II, with ITAM-bearing adapters DAP12, FcRγ chain on peritoneal macrophages (2007)
  • Author(s): Nakahashi C, Tahara-Hanaoka S, et.al.
  • Journal Title: J Immunol 178 Pages: 765-70
  • Peer Reviewed
• [Journal Article] A critical role of LFA-1 in the development of Th17 cells, induction of experimental autoimmune encephalomyelitis (2007)
  • Author(s): Wang Y, Tahara-Hanaoka S, et.al.
  • Journal Title: Biochem Biophys Res Commun 23,353(4) Pages: 857-862
  • Peer Reviewed
• [Journal Article] A human mutant CD4 molecule resistant to HIV-1 binding restores helper T-lymphocyte functions in murine CD4-deficient mice (2007)
  • Author(s): Kim DK, Tahara-Hanaoka S, Shinohara N, Nakauchi H.
  • Journal Title: Exp Mol Med 28,39(1) Pages: 1-7
  • Peer Reviewed
• [Journal Article] Activation of neutropbils by a novel thiggering immunoglobulin-like receptor MAIR-IV. (2007)
  • Author(s): Nakano T, et. al.
  • Journal Title: Molecular immunology 45(1) Pages: 289-94
  • Peer Reviewed
• [Journal Article] Dual assemblies of an activating immune receptor, MAIR-II, With ITAM-bearing adapters DAP12 and FcR chain on peritoneal macrophages. (2007)
  • Author(s): Nakahashi C, et. al.
  • Journal Title: Journal of Immunology 15;178(2) Pages: 765-70
  • Peer Reviewed
• [Journal Article] A critical role of LEA-1 in the development of Th17 cells and induction of experimental autoimmune encephalomyelitis. (2007)
  • Author(s): Wang Y, et. al.
  • Journal Title: Biochem Biophys Res Commun 23;353 Pages: 857-62
  • Peer Reviewed
• [Presentation] Involvement of DNAM-1 (CD226) in development of graft-versus-host disease (2008)
  • Author(s): Nabekura T et.al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Prolonged survival of MAIR-I (CD300a) -deficient mice from acute septic peritonitis (2008)
  • Author(s): Nakahashi-Oda C et.al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Yamashita Y et.al. A novel role of CD155 as a co-stimulatory moleculeon CD4+ helper T cells in mice (2008)
  • Author(s): Yamashita Y et.al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Prolonged survival of MAIR-I (CD300a)-deficient mice from acute septic peritonitis (2008)
  • Author(s): Nakahashi-Oda C et. al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] A novel role of CD155 as a co-stimulatory molecule on CD4+ helper T cells in mice (2008)
  • Author(s): Yamashita Y et. al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Identification and characterization of a novel inhibitory immunoglobulin-like recetor, MBIR (2008)
  • Author(s): Tahara-Hanaoka S et.al.
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] MAIR-II (CD300d) amplifies TLRs-mediated inflammation, is crucial mediator of murine sepsis model (2008)
  • Author(s): Totsuka N et.al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Negative regulation of IgA production by the Fcα/μR, an Fc receptor for IgA, IgM (2008)
  • Author(s): Kurita N et.al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Increased anti-PC natural IgM production, resistance against Streptococcus pneuoniae infection in mice deficient in Fca/mR, an Fc receptor for IgA, IgM (2008)
  • Author(s): Honda S et.al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Identification and characterization of a novel inhibitory immunoglobulin-like recetor, MBIR (2008)
  • Author(s): Tahara-Hanaoka-S et. al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] MAIR-II (CD300d) amplifies TLRs-mediated inflammation and is crucial mediator of murine sepsis model (2008)
  • Author(s): Totsuka N et. al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Negative regulation of IgA production by the Fc α/R, an Fc receptor for IgA and IgM (2008)
  • Author(s): Kurita N et. al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Increased anti-PC natural IgM production and resistance against Streptococcus pneuoniae infection in mice deficient in Fca/mR, an Fc receptor for IgA and IgM (2008)
  • Author(s): Honda S et. al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Accelerated tumor growth in mice deficient in DNAM-1 (CD226) receptor. (2008)
  • Author(s): Iguchi A et.al.
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Accelerated tumor growth in mice deficient in DNAM-1 (CD226) receptor (2008)
  • Author(s): Iguchi A et. al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Negative regulation of IgA production by the Fc receptor, an Fc receptor for IgA, IgM (2008)
  • Author(s): Kurita N et.al.
  • Organizer: 第3回JSI-RCAI免疫ワークショップ
  • Place of Presentation: 理化学研究所・横浜研究所
  • Year and Date: 2008-03-14
• [Presentation] Negative regulation of IgA production by the Fcα/μ recepto, an Fc receptor for IgA and IgM. (2008)
  • Author(s): KURITA Naoki, HONDA Shin-ichiro, CHO Yukiko, USUI Kenta, TAKESHITA Kie, TAHARA-HANAOKA Satoko, SHIBUYA Kazuko and SHIBUYA Akira.
  • Organizer: 第3回JSI-RCAI免疫ワークショップ
  • Place of Presentation: 理化学研究所・横浜研究所
  • Year and Date: 2008-03-14
• [Presentation] Activation of neutrophils by a novel triggering immunoglobulin -like receptor, MAIR-IV (2008)
  • Author(s): Nakano T et.al.
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone, USA
  • Year and Date: 2008-02-25
• [Presentation] Activation of neutrophils by a novel triggering immunoglobulin-1ike receptor MAIR-IV (2008)
  • Author(s): Takako Nakano, Satoko Tahara-Hanaoka, Chigusa Nakahashi-Oda, Ismail Can, Naoya Totsuka, Kaori Hitomi, Satoru Someya, Shin-ichiro Honda, Kazuko Shibuya, and Akira Shibuya.
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone USA
  • Year and Date: 2008-02-25
• [Presentation] ITIMを持つ免疫受容体MAIR-Vの機能解析 (2007)
  • Author(s): Can I et.al.
  • Organizer: 第69回日本血液学会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-10-12
• [Presentation] 好中球の活性化を制御する新規ペア型受容体MAIR-IV (2007)
  • Author(s): Nakano T et.al.
  • Organizer: 第69回日本血液学会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-10-12
• [Presentation] ITIMを持つ免疫受容体MAIR-Vの機能解析 (2007)
  • Author(s): Ismail Can, Satoko Tahara-Hanaoka, Shin-ichiro Honda, Kazuko Shibuya, Akira Shibuya.
  • Organizer: 第69回 日本血液学会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-10-12
• [Presentation] 好中球の活性化を制御する新規ペア型受容体MAIR-IV (2007)
  • Author(s): 中埜 貴子、田原 聡子、中橋 ちぐさ、Ismail Can、戸塚 直也、本多 伸一郎、渋谷 和子、渋谷 彰
  • Organizer: 第69回 日本血液学会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-10-12
• [Presentation] ヘルパーT細胞免疫応答におけるDNAM-1の役割 (2007)
  • Author(s): Kai H et.al.
  • Organizer: KTCC.
  • Place of Presentation: 京都
  • Year and Date: 2007-06-16
• [Presentation] ヘルパーT細胞免疫応答におけるDNAM-1の役割 (2007)
  • Author(s): 甲斐平康、渋谷和子、井口研子、田原聡子、本多伸一郎、渋谷彰
  • Organizer: KTCC
  • Place of Presentation: 京都
  • Year and Date: 2007-06-16
• [Presentation] Control of Germinal Center Reaction against TIndependent Antigens by the Fc(alpha)/(mu)Receptor. (2007)
  • Author(s): Akira Shibuya, Ukiko Cho, Satoko Tahara-Hanaoka, kazuko Shibuiya, Shin-ichiro Honda.
  • Organizer: 94th Annual Meeting The American Association of Immunologists.
  • Place of Presentation: USA
  • Year and Date: 2007-05-21
• [Presentation] Physical and functional association of MAIR-II with ITAM containing adapters FcRγ chain as well as DAP12 in activated macroph (2007)
  • Author(s): A Shibuya, C Nakahashi, N Totsuka, S Tahara-Hanaoka.
  • Organizer: Keystone Symposia
  • Place of Presentation: Colorado, USA
  • Year and Date: 2007-04-14
• [Presentation] IgM suppresses humoral immune responses against T-independent antigens via the Fcα/μR (2007)
  • Author(s): Honda S. et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Caspase-independent cell death by MAIR-V (CD300LF) immunoglobulin-like receptor on myeloid cells (2007)
  • Author(s): Can I et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] A critical role of DNAM-1 in tumor immunosurveillance (2007)
  • Author(s): Iguchi A et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Analysis for the variants of FcαμR, a novel Fc receptor for IgA, IgM, expressed in the kidney, testis (2007)
  • Author(s): Usui K et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Negative regulation of IgA production by the Fca/mR, an Fc receptor for IgA, IgM (2007)
  • Author(s): Kurita N et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会.
  • Place of Presentation: 東京
• [Presentation] A critical role of LFA-1 in the development of Th17 cells, induction of experimental autoimmune encephalomyelitis (2007)
  • Author(s): Shibata K et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Critical role of DNAM-1 (CD226) in the development of Th1 cells (2007)
  • Author(s): Kai H et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] A novel triggering neutrophil receptor, MAIR-IV, transduces activating signal via FcRγ-independent as well as dependent pathways (2007)
  • Author(s): Nakano T et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] ITIM-bearing Immunoglobulin-like receptor, MAIR-I (CD300a) modulates the inflammatory responses in murine sepsis (2007)
  • Author(s): Nakahashi-Oda C et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Molecular characterization of Fcα/μR, a novel Fc receptor for IgA, IgM (2007)
  • Author(s): Cho Y et.al.
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] IgM suppresses humoral immune responses against T-independent antigens via the Fcα/μR (2007)
  • Author(s): HONDA Shin-ichiro, CHO Yukiko, USUI Kenta, KURITA Naoki, TAKESHITA Kie^1, TAKAHASHI Satoru, KINOSHITA Taroh, FUJITA Teizo、 TAHARA-HANAOKA Satoko, SHIBUYA Kazuko and SHIBUYA Akira.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Caspase-independent cell death by MAIR-V(CD300LF), immunoglobulin-like receptor on myeloid cells (2007)
  • Author(s): Ismail Can, Satoko Tahara-Hanaoka, Chigusa Nakahashi, Takako Nakano, Kaori Hitomi, Shin-ichiro Honda, Kazuko Shibuya, Akira Shibuya
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] A critical role of DNAM-1 in tumor immunosurveillance (2007)
  • Author(s): Iguchi Akiko, SHIBUYA Kazuko, KAI Hirayasu, TAHARA-HANAOKA Satoko, HONDA Shinichiro, SHIBUYA Akira.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Analysis for the variants of Fcα/μR, a novel Fc receptor for IgA and IgM, expressed in the kidney and testis. (2007)
  • Author(s): USUI Kenta, HONDA Shin-ichiro, KURITA Naoki, MIYAMOTO Akitomo, CHO Yukiko, TAHARA-HANAOKA Satoko, SHIBUYA Kazuko, SHIBUYA Akira
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Negative regulation of IgA production by the Fca/mR, an Fc receptor for IgA and IgM. (2007)
  • Author(s): KURITA Naoki, HONDA Shin-ichiro, CHO Yukiko, USUI Kenta, TAKESHITA Kie, TAHARA Satoko, SHIBUYA Kazuko, SHIBUYA Akira.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] A critical role of LFA-1 in the development of Th17 cells and induction of experimental autoimmune encep halomyelitis. (2007)
  • Author(s): SHIBATA Kai^1, WANG Yinan^1, KAI Hirayasu^1, TAHARA-HANAOKA Satoko^1, HONDA Shin-ichiro^1, SHIBUYA Akira^1, SHIBUYA Kazuko.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Critical role of DNAM-1(CD226) in the development of Th1 cells. (2007)
  • Author(s): KAI Hirayasy, IGUCHI Akiko, WANG Yinan, YAMASHITA Yumi, YASUI Teruhito, KIKUTANI Hitoshi, TAHARA-HANAOKA Satoko HONDA Shin-ichiro, SHIBUYA Akira, SHIBUYA Kazuko。
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] A novel triggering neutrophil receptor, MAIR-IV, transduces activating signal via FcRγ-independent as well as dependent pathways. (2007)
  • Author(s): Takako Nakano, Satoko Tahara-Hanaoka, Chigus a Nakahashi, Can Ismail, Naoya Totsuka, Kaori Hitomi, Satoru Someya, Shin-ichiro Honda, Kazuko Shibuya, Akira Shibuya.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] ITIM-bearing Immunoglobulin-like receptor, MAIR-I(CD300a) modulates the inflammatory responses in murine sepsis. (2007)
  • Author(s): Chigusa Nakahashi-Oda, Satoko Tahara-Hanaoka, Kaori Hitomi, Takako Nakano, Can Ismail, Naoya Totsuk a, Teruhito Yasui, Hitoshi Kikutani, Shin-ichiro Hond Kazuko Shibuya, Akira Shibuya.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Molecular characterization of Fcα/μR, a novel Fc receptor for IgA and IgM. (2007)
  • Author(s): CHO Yukiko, HONDA Shin-ichiro, USUI Kenta, KURITA Naoki, TAKESHITA Kie, TAHARA-HANAOKA Satoko, SHIBUYA Kazuko and SHIBUYA Akira
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Book] 改訂版「抗体実験マニュアル」実験医学別冊 (2008)
  • Author(s): 田原聡子、渋谷彰
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/immunology/immunol.index.html
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/immunology/immunol.index.html