Role of ASC, which regulates inflammation and cell death, in Helicobacter pylori infection

• Project/Area Number: 19590385
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Shinshu University
• Principal Investigator: TANIGUCHI Shun-ichiro Shinshu University, 医学系研究科, 教授 (60117166)
• Co-Investigator(Kenkyū-buntansha): TSUTSUI Hiroko 兵庫医科大学, 医学部, 教授 (40236914) ; KOBAYASHI Motohiro 信州大学, 医学系研究科, 講師 (00362137) ; SAGARA Junji 信州大学, 医学部, 教授 (10225831) ; ITANO Naoki 信州大学, 医学系研究科, 准教授 (40257712)
• Co-Investigator(Renkei-kenkyūsha): TSUTSUI Hiroko 兵庫医科大学, 医学部, 教授 (40236914)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 腫瘍 / 自然免疫 / ASC / 炎症 / 感染 / ピロリ菌 / 細胞死

Research Abstract

炎症と細胞死を制御するASCのピロリ菌感染における役割を解明するために、ASC欠損マウスにピロリ菌を感染させた所、野生型マウスに比し、感染は高率であったものの、炎症はより軽度であるという結果を得た。宿主防御反応に重要と思われるIL-1b産生がASC欠損故に無く, 特にIL-18の産生も無くそれで誘導されるIFN-gが産生されなかったためという分子機序を示唆することが出来た。

Research Products

• [Journal Article] Role of ASC in the Mouse Model of Helicobacter pylori Infection (2008)
  • Author(s): Benoit BN, Kobayashi M, Kawakubo M, Takeoka M, Sano K, Zou J, Itano N, Tsutsui H, Noda T, Fukuda M, Nakayama J, Taniguchi S
  • Journal Title: J.Histochem & Cytochem 271 Pages: 9-12
  • Peer Reviewed
• [Journal Article] Critical role of bone marrow apoptosis-associated speck-like protein, an inflammasome adaptor molecule, in neointimal formation after vascular injury in mice (2008)
  • Author(s): Yajima N, Takahashi M, Morimoto H, Shiba Y, Takahashi Y, Masumoto J, Ise H, Sagara J, Nakayama J, Taniguchi S, Ikeda U
  • Journal Title: Circulation 17 Pages: 3079-3087
  • Peer Reviewed
• [Journal Article] Critical role of bone marrow apoptosis-associated speck-like protein, an inflammasome adaptor molecule, in neointimal formation after vascular injury in mice. 8 (2008)
  • Author(s): Yajima N, Takahashi M, Morimoto H, Shiba Y, Takahashi Y, Masumoto J, Ise H, Sagara J, Nakayama J, Taniguchi S, Ikeda U.
  • Journal Title: Circulation. 17 Pages: 3079-3087
  • Peer Reviewed
• [Journal Article] Role of ASC in the Mouse Model of Helicobacter pylori. Infection (2008)
  • Author(s): Benoit BN, Kobavashi M. Kawakubo M, Takeoka M, Sano K, Zou J, Itano N. Tsutsui H, Noda T, Fukuda M, Nakayama J, Tanjguchi S
  • Journal Title: J. Histochem & Cytochem. 271 Pages: 9-12
  • Peer Reviewed
• [Journal Article] Conyribution of TIR domain-containing adapter inducing IFN-β mediated IL-18 release to LPS-induced liver injury in mice. (2008)
  • Author(s): Imamura M, Tsutsui H, Yasuda K, Yumikura -Futatsugi S, Mitani K, Uchiyama R, Hayashi S, Akira S, Taniguchi S, Nico Van Rooijen, Jurg Tschopp, Yamamoto T, Fujimoto J, Nakanishi K.
  • Journal Title: J. Hepatology (In press)
  • Peer Reviewed
• [Journal Article] Significance of Tumor-Associated Stroma in Promotion of Intratumoral Lymphangiogenesis: Pivotal Role of a Hyaluronan-Rich Tumor Microenviromment. (2008)
  • Author(s): Koyama H, Kobayashi N, Harada M, Takeoka M, Kawai Y, Sano K, Fujimori M, Amano, J., Ohhashi, T., Kannagi, R., Kimata, K., Taniguchi, S., Itano, N.
  • Journal Title: Am. J. Pathol. 172 Pages: 179-193
  • Peer Reviewed
• [Journal Article] Development of spontaneous optic neuropathy in NF-kBp50-deficient mice ; Requirement for NF-kBp50 in ganglion cell survival (2007)
  • Author(s): Takahashi Y, Katai N, Murata T, Taniguchi S, Hayashi T
  • Journal Title: Neuropath, Applied Neurobiol 33(6) Pages: 692-705
  • Peer Reviewed
• [Journal Article] Regulatory molecules involved in inflammasome formation with special reference to a key mediator protein, ASC (2007)
  • Author(s): Taniguchi S, Sagara J
  • Journal Title: Semin Immunopathol 29(3) Pages: 231-238
  • Peer Reviewed
• [Journal Article] Regulatory molecules involved in inflammasome formation with special reference to a key mediator protein, ASC. (2007)
  • Author(s): Taniguchi S, Sagara J.
  • Journal Title: Semin Immunopathol. 29(3) Pages: 231-238
  • Peer Reviewed
• [Journal Article] Development of spontaneous optic neuropathy in NF-kBp50-deficient mice; Requirement for NF-kBp50 in ganglion cell survival. (2007)
  • Author(s): Takahashi Y, Katai N, Murata T, Taniguchi S, Hayashi T
  • Journal Title: Neuropath, Applied Neurobiol. 33(6) Pages: 692-705
  • Peer Reviewed
• [Journal Article] Nico Van Rooijen, Jurg Tschopp, Yamamoto T, Fujimoto J, Nakanishi K
  • Author(s): Imamura M, Tsutsui H, Yasuda K, Yumikura-Futatsugi S, Mitani K, Uchiyama R, Hayashi S, Akira S, Taniguchi S
  • Journal Title: Conyribution of TIR domain-containingadapter inducing IFN-β mediated IL-18 release to LPS-induced liver injury in mice (In press)
• [Presentation] ASC regulates tumor immunity by IL-18/IFN-gamma axis. (2008)
  • Author(s): 鄒健, 二好征司, 小林宣隆, ベノイトベカレ ニュマ, 竹岡みち子, 板野直樹, 谷口俊一郎
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Role of ASC protein in the mouse model of Helicobacter pyliro infection. (2008)
  • Author(s): BEKALE NGUEMA BENOIT, Michiko Takeoka, Naoki Itano, JunNakayama, Shun'ichiro Taniguchi
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Chronic High-Intensity Walking Exercise Alters DNA Methylation in Normal Middle-Aged and Older Subjects to Younger State. (2008)
  • Author(s): Koki Nakajima, Michiko Takeoka, Masyuki Mori, Akihiro Sakurai, Hiroshi Nose, Keiichi Higuchi, Naoki Itano, Shun'ichiro Taniguchi
  • Organizer: 7th World Congress on Aging and Physical Activity
  • Place of Presentation: Tsukuba
  • Year and Date: 2008-07-27
• [Presentation] ウォーキング運動のエピジェネティツク効果-ASC遺伝子のメチル化に着目して-(第3報) (2008)
  • Author(s): 中島弘毅, 竹岡みち子, 森政之, 横井晃洋, 能勢博, 樋口京一, 板野直樹, SUGRC, 谷口俊一郎
  • Organizer: 第31回日本基礎老化学会
  • Place of Presentation: 松本
  • Year and Date: 2008-06-12
• [Presentation] ASC regulates tumor immunity byIL-18/IFN-gamma axis (2008)
  • Author(s): 鄒健、三好征司、小林宣隆、ベノイトベカレニュマ、竹岡みち子、板野直樹、谷口俊一郎
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Jun Nakayama, Shun'ichiro Taniguchi Role of ASC protein in the mouse model of Helicobacter pyliro infection (2008)
  • Author(s): BEKALE NGUEMA BENOIT, Michiko Takeoka, Naoki Itano
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Chronic High-Intensity Walking Exercise Alters DNA Methylation in Normal Middle-Aged and Older Subjects to Younger State (2008)
  • Author(s): Koki Nakajima, Michiko Takeoka, Masyuki Mori,Akihiro Sakurai,Hiroshi Nose, Keiichi Higuchi, Naoki Itano and Shun'ichiro Taniguchi
  • Organizer: 7th World Congress on Aging and Physical Activity
  • Place of Presentation: Tsukuba
• [Presentation] ウォーキング運動のエピジェネティック効果-ASC遺伝子のメチル化に着目して-(第3報) (2008)
  • Author(s): 中島弘毅, 竹岡みち子, 森政之, 櫻井晃洋, 能勢博, 樋口京一, 板野直樹, SUGRC, 谷口俊一郎
  • Organizer: 第31回日本基礎老化学会
  • Place of Presentation: 松本
• [Presentation] カスペース-1活性化シグナル経路のアダプター分子ASCスプライシング変異体(vASC)の機能解析 (2007)
  • Author(s): 松下一彦、相良淳二、谷口俊一郎
  • Organizer: 第37回日本免疫学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] ASC deficiency enhances autophagy for cell survival. (2007)
  • Author(s): Takahashi Y, Takahashi M, Itano N, Sagara J, Taniguchi S.
  • Organizer: 66th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama