Functional analyses of mouse lectin SIGNR family

• Project/Area Number: 19590389
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: TAKAHARA Kazuhiko Kyoto University, 生命科学研究科, 講師 (90301233)
• Co-Investigator(Kenkyū-buntansha): INABA Kayo 京都大学, 生命科学研究科, 教授 (00115792)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: レクチン / 単クローン抗体 / 微生物 / マクロファージ / 樹状細胞 / 単球 / 糖鎖 / モノクローナル抗体

Research Abstract

マウスレクチンSIGNRファミリーはヒトDC-SIGNのホモログとして同定された遺伝子群であり、病原微生物を認識する事が明らかになりつつある. この内で、SIGNR3はキャンディダ症原因微生物を認識する事が示されているが、特異抗体が無く、生体内における発現様式は不明であった. 本研究では、SIGNR3を認識する単クローン抗体を作製し、これを用いて生体内における発現を検討すると共に、SIGNR3が皮膚マクロファージおよび樹状細胞の一部、さらにそれらの前駆細胞である血中単球に発現する事などを突き止めた.

Research Products

• [Journal Article] Mycobacterium abscessus activates the macrophage innate immune response via a physical and functional interaction between TLR2 and dectin-1 (2008)
  • Author(s): Shin DM, Yang CS, Yuk JM, Lee JY, Kim KH, Shin SJ, Takahara K, Lee SJ, Jo EK.
  • Journal Title: Cell Microbiol Pages: 1608-1621
  • Peer Reviewed
• [Journal Article] Mycobacterium abscessus activates the macrophage innate immune response via a physical and functional interaction between TLR2 and dectin-1 (2008)
  • Author(s): Shin DM
  • Journal Title: Cell Microbiol. 10(8) Pages: 1608-1621
  • Peer Reviewed
• [Journal Article] Cytotoxic T-cell-mediated defense against infections in human liver transplant receipient. (2007)
  • Author(s): Koichi Tanaka
  • Journal Title: Liver Transplant. 324 Pages: 48-62
  • Peer Reviewed
• [Journal Article] Production of monoclonal antibodies that recognize the extracellular domain of mouse Langerin/CD207. (2007)
  • Author(s): Cheolho Cheong
  • Journal Title: J. Immunol. Methods 324 Pages: 48-62
  • Peer Reviewed
• [Journal Article] CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells. (2007)
  • Author(s): Kenji Kabashima
  • Journal Title: Am J Pathol. 171 Pages: 1249-1257
  • Peer Reviewed
• [Journal Article] Dendritic cells are specialized accessory cells along with TGF-β for the differentiation of Foxp3+CD4+regulatory T cells from peripheral Foxp3-precursors. (2007)
  • Author(s): Sayuri Yamazaki
  • Journal Title: Blood 110 Pages: 4293-4302
  • Peer Reviewed
• [Presentation] Role of SIGN-related molecules in innate immune responses (2009)
  • Author(s): 高原和彦
  • Organizer: CREST国際シンポジウム「獲得免疫と糖鎖生物学」
  • Place of Presentation: かずさ
  • Year and Date: 2009-03-24
• [Presentation] referential migration of Ly6ChighSIGNR3 monocyte subset into peripheral lymph nodes in the steady state (2008)
  • Author(s): 長岡孝治
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Preferential migration of Ly6ChighSIGNR3 monocyte subset into peripheral lymph nodes in the steady state (2008)
  • Author(s): 長岡孝治
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Recognition property of SIGNR1 for C. albicans mannoprotein to enhances TNF- α production and candidacidal activity (2008)
  • Author(s): 高原和彦
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Recognition property of SIGNRl for C. albicans mannoprotein to enhances TNF-α production and candidacidal activity (2008)
  • Author(s): 高原和彦
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] C-type lectin SIGNR1 recognizing N-glycan α-mannan enhances nitric oxide production and candidacidal activity of RAW264.7 cells against C. albicans (2008)
  • Author(s): 高原和彦
  • Organizer: The Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路島
  • Year and Date: 2008-09-09
• [Presentation] C-type lectin SIGNRl recognizing N-glycan α-mannan enhances nitric oxide production and candidacidal activity of RAW264. 7 cells against C, albicans (2008)
  • Author(s): 高原和彦
  • Organizer: The Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路島
  • Year and Date: 2008-09-09
• [Presentation] Role of SIGNR1 in abti-microbial responses、US-Japan Cooperative Medical Science Program (2008)
  • Author(s): 稲葉カヨ
  • Organizer: Aging & Immunosenescence
  • Place of Presentation: 米国サンフランシスコ
  • Year and Date: 2008-06-20
• [Presentation] Role of SIGNRl in abti-microbial responses (2008)
  • Author(s): 稲葉カョ
  • Organizer: US-Japan Cooperative Medical Science Program, Aging & Immunosenescence
  • Place of Presentation: San Francisco
  • Year and Date: 2008-06-20
• [Presentation] Precise expression analysis of mouse DCIR1 using monoclonal antibody (2007)
  • Author(s): 吉田洋子
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] Expression of SIGNR3 in mmature monocytic cells during inflammatory response (2007)
  • Author(s): 長岡孝治
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] Expression of SIGNR3 in immature monocytic cells during inflammatory response (2007)
  • Author(s): 長岡 孝治
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] Precise expression analysis of mouse DCIRI using monoclonal antibody (2007)
  • Author(s): 吉田 洋子
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] 各種名の物質およびアスベストによる細胞のサイトカイン産生 (2007)
  • Author(s): 高原和彦
  • Organizer: 日本免疫毒性学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2007-09-21
• [Presentation] Role of SIGNR1 in the recognition and response to pathogenic fungus (2007)
  • Author(s): 高原和彦
  • Organizer: The Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路島
  • Year and Date: 2007-09-04
• [Remarks]
  • URL: http://zoo.zool.kyoto-u.ac.jp/imm/
• [Remarks] 高原和彦、長岡孝治、稲葉カヨ(2008)樹状細胞に発現するC型レクチンの機能とシグナル伝達. 実験医学. 26, 3187-3196(日本語総説. 査読なし).
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://zoo.zool.kyoto-u.ac.jp/imm/
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://zoo.zool.kyoto-u.ac.jp/imm/