Identification of cytogenesis of pancreatic cancer in Ras transgenic rats

• Project/Area Number: 19590401
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Nagoya City University
• Principal Investigator: FUKAMACHI Katsumi Nagoya City University, 大学院・医学研究科, 助教 (90381798)
• Co-Investigator(Kenkyū-buntansha): TSUDA Hiroyuki 名古屋市立大学, 大学院・医学研究科, 特任教授 (30094387) ; ALEXANDER David 名古屋市立大学, 大学院・医学研究科, 研究員 (00335267) ; 津田 洋幸 名古屋市立大学, 大学院・医学研究科, 教授 (10163809) ; ALEXANDER David 名古屋市立大学, 大学院・医学研究科, 客員教授 (90381805)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 腫瘍 / 膵管がん / Ras / トランスジェニックラット / ras / 膵がん

Research Abstract

Pancreatic ductal adenocarcinoma (PDA) is one of the most debilitating malignancies in humans. A thorough understanding of the cytogenesis of this disease will aid in establishing successful treatments. We have developed an animal model which uses adult ras^<G12V> transgenic rats in which oncogene expression is regulated by the Cre/loxP system. We showed that the origin of PDA is duct, intercalated duct, and centroacinar cells, but not acinar cells.

Research Products

• [Journal Article] Mature acinar cells are refractory to carcinoma development by targeted activation of Ras oncogene in adult rats. (2010)
  • Author(s): Tanaka, H., Fukamachi, K., Futakuchi, M., Alexander, D.B., Long, N., Tamamushi, S., Minami, K., Seino, S., Ohara, H., Joh, T., Tsuda, H.
  • Journal Title: Cancer Sci 101 Pages: 341-346
  • NAID: 10026587596
  • Peer Reviewed
• [Journal Article] Mature acinar cells are refractory to carcinoma development by targeted activation of Ras oncogene in adult rats (2010)
  • Author(s): Tanaka, H., et al
  • Journal Title: Cancer Sci 101 Pages: 341-346
  • Peer Reviewed
• [Journal Article] An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas. (2009)
  • Author(s): Fukamachi, K., Tanaka, H., Hagiwara, Y., Ohara, H., Joh, T., Iigo, M., Alexander, D.B., Xu, J., Long, N., Takigahira, M., Yanagihara, K., Hino, O., Saito, I., Tsuda, H.
  • Journal Title: Biochem Biophys Res Commun 390 Pages: 636-641
  • Peer Reviewed
• [Journal Article] An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas (2009)
  • Author(s): Fukamachi, K., et al
  • Journal Title: Biochem Biophys Res Commun 390 Pages: 636-641
  • Peer Reviewed
• [Journal Article] Purple corn color suppresses Ras protein level and inhibits 7, 12-dimethylbenz[a]anthracene-indu ced mammary carcinogenesis in the rat. (2008)
  • Author(s): Fukamachi, K., Imada, T., Ohshima, Y., Xu, J., Tsuda, H.
  • Journal Title: Cancer Sci 99 Pages: 1841-1846
  • Peer Reviewed
• [Journal Article] Purple corn color suppresses Ras protein level and inhibits 7, 12-dimethylbenz[a]anthracene-induced mammary carcinogenesis in the rat (2008)
  • Author(s): Fukamachi, K., et al.
  • Journal Title: Cancer Sci 99 Pages: 1841-1846
  • NAID: 10024011232
  • Peer Reviewed
• [Journal Article] Possible enhancing activity of diacylglycerol on 4-nitroquinoline 1-oxide induced carcinogenesis of the tongue in human c-Ha-ras proto-oncogene transgenic rats. (2007)
  • Author(s): Tsuda, H., Iigo, M., Takasuka, N., Ueda, S., Ohshima, Y., Fukamachi, K., Shirai, T., Hirano, S., Matsuda, E., Wakabayashi, K.
  • Journal Title: Food Chem Toxicol 45 Pages: 1013-1019
  • Peer Reviewed
• [Journal Article] Possible application of human c-Ha-ras proto-oncogene transgenic rats in a medium-term bioassay model for carcinogens. (2007)
  • Author(s): Ohnishi, T., Fukamachi, K., Ohshima, Y., Jiegou, X., Ueda, S., Iigo, M., Takasuka, N., Naito, A., Fujita, K., Matsuoka, Y., Izumi, K., Tsuda, H.
  • Journal Title: Toxicol Pathol 35 Pages: 436-443
  • Peer Reviewed
• [Journal Article] Possible Application of Human c-Ha-ras Proto-Oncogene Transgenic Rats in a Medium-Term Bioassay Model for Carcinogens (2007)
  • Author(s): Ohnishi T, et. al.
  • Journal Title: Toxicol Pathol 35 Pages: 436-443
  • Peer Reviewed
• [Journal Article] Possible enhancing activity of diacylglycerol on 4-nitroquinoline 1-oxide induced carcinogenesis of the tongue in human c-Ha-ras proto-oncogene transgenic rats (2007)
  • Author(s): Tsuda, H, et. al.
  • Journal Title: Food Chem Toxicol 45 Pages: 1013-1019
  • Peer Reviewed
• [Presentation] Possible identification of cytogenesis of pancreas cancer and lung cancer in the rat (2009)
  • Author(s): Hajime Tanaka, Yutaka Ohshima, Katsumi Fukamachi, David Alexander, Mitsuru Futakuchi, Takashi Joh, Hiroyuki Tsuda
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] Possible identification of cytogenesis of pancreas cancer and lung cancer in the rat (2009)
  • Author(s): 田中創始, 他
  • Organizer: 68回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] Adult pancreatic acinar cells are refractory to transformation by Ras oncogene (2008)
  • Author(s): Hajime Tanaka, Katsumi Fukamachi, David Alexander, Mitsuru Futakuchi, Takashi Joh, Hiroyuki Tsuda
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Adult pancreatic acinar cells are refractory o transformation by Ras oncogene (2008)
  • Author(s): Tanaka H., et al.
  • Organizer: 第67回 日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Patent(Industrial Property Rights)] ヒト変異型K-ras(K-rasV12)遺伝子をコンディショナルに発現するトランスジェニック非ヒト哺乳動物及びその用途 (2007)
  • Inventor(s): 津田洋幸, 深町勝巳, デイビッド・ビー・アレキサンダー
  • Industrial Property Rights Holder: 名古屋市立大学
  • Industrial Property Number: 2007-243313
  • Filing Date: 2007-09-20
• [Patent(Industrial Property Rights)] ヒト変異型Kras遺伝子をコンディショナルに発現するトランスジェニック非ヒト哺乳動物及びその用途 (2007)
  • Inventor(s): 津田洋幸、深町勝巳、Alexander David
  • Industrial Property Rights Holder: 名古屋市立大学
  • Filing Date: 2007-09-20