Comparative analysis of hnRNP A2/B1 isoform proteins to clarify the telomeric paradox in cancer cells

• Project/Area Number: 19590403
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kyorin University
• Principal Investigator: KAMMA Hiroshi Kyorin University, 医学部, 准教授 (10195191)
• Co-Investigator(Kenkyū-buntansha): 藤原 正親 杏林大学, 医学部, 助教 (20407026)
• Co-Investigator(Renkei-kenkyūsha): FUJIWARA Masachika 杏林大学, 医学部, 助教 (20407026)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 癌 / テロメア / hnRNP A2 / B1 / 核酸 / 病理学 / 生殖細胞

Research Abstract

癌のテロメアパラドクスの解明に向けて、hnRNP A2、B1、B0 蛋白の比較解析を行った。B1 蛋白はA2蛋白に比べ、癌細胞で発現が亢進するが、癌細胞の中でも特に、テロメラーゼによらないテロメア伸長(ALT)を示す癌細胞でより高い事を示した。B1 とB0 蛋白は、より特異的に一本鎖テロメアDNA と結合することを示した。各蛋白間の差は、そのアミノ酸構造とユビキチン化による蛋白代謝の差によって生じる可能性を示した。

Research Products

• [Journal Article] Expression of stem cell factor (SCF), a KIT ligand, in gastrointestinal stromal tumors (GIST) : A potential marker for tumor proliferation (2008)
  • Author(s): Hirano K, Shishido-Hara Y, Kitazawa A, Kojima K, Sumiishi A, Umino M, Kikuchi F, Sakamoto A, Fujioka Y, Kamma H
  • Journal Title: Pathol Res Pract. 204 Pages: 799-807
  • Peer Reviewed
• [Journal Article] Decreased expression of the thyroid-stimulating hormone receptor in poorly-differentiated carcinoma of the thyroid (2008)
  • Author(s): Matsumoto H, Sakamoto A, Fujiwara M, Yano Y, Shishido-Hara Y, Fujioka Y & Kamma H
  • Journal Title: Oncol Rep. 19 Pages: 1405-1411
  • Peer Reviewed
• [Journal Article] Cyclic AMP-mediated growth suppression and MAPK phosphorylation in thyroid papillary carcinoma cells (2008)
  • Author(s): Matsumoto M, Sakamoto A, Fujiwara M, Yano Y, Shishido-Hara Y, Fujioka Y, Kamma H
  • Journal Title: Molr Med Rep. 1 Pages: 245-249
  • Peer Reviewed
• [Journal Article] 甲状腺低分化癌の組織所見のコンセンサス (2008)
  • Author(s): 菅間博
  • Journal Title: 病理と臨床 26(2) Pages: 629-636
• [Journal Article] 癌前駆病変としての乳管内増殖性病変-WHO分類の立場からの解説- (2008)
  • Author(s): 菅間博、中山英里、宍戸-原由紀子
  • Journal Title: 診断病理 25 Pages: 57-63
  • NAID: 10021251960
  • Peer Reviewed
• [Journal Article] Expression of stem cell factor (SCF), a KIT ligand, in gastrointestinal stromal tumors (GIST) (2008)
  • Author(s): Hirano K
  • Journal Title: Pathol Res Pract. 204 Pages: 799-807
  • Peer Reviewed
• [Journal Article] Decreased expression of the thyroid-stimulating hormone receptor in poorly-differentiated carcinoma of the thyroid. (2008)
  • Author(s): Matsumoto H
  • Journal Title: Oncol Rep. 19 Pages: 1405-11
  • Peer Reviewed
• [Journal Article] Cyclic AMP-mediated growth suppression and MAPK phosphorylation in thyroid papillary carcinoma cells (2008)
  • Author(s): Matsumoto H
  • Journal Title: Molecular.Medicine Report 1 Pages: 245-249
  • Peer Reviewed
• [Journal Article] Growth suppression of thyroid cancer cells by adenylcyclase activator (2007)
  • Author(s): Yano Y, Kamma H, Matsumoto H, Fujiwara M, Bando H, Hara H, Yashiro T, Ueno E, Ito K, Uchida K
  • Journal Title: Oncol Rep. 18(2) Pages: 441-445
  • Peer Reviewed
• [Journal Article] The importance of postoperative radiotherapy against polymorphous low-grade adenocarcinoma of the parotid gland : case report and review of the literature (2007)
  • Author(s): Uemaetomari I, Tabuchi K, Tobita T, Tsuji S, Wada T, Kamma H, Hara A
  • Journal Title: Tohoku J Exp Med 211(3) Pages: 297-302
  • Peer Reviewed
• [Journal Article] Role of ultrasonography and MRI in the detection of wide intraductal component of invasive breast cancer -a prospective study (2007)
  • Author(s): Sundararajan S, Tohno E, Kamma H, Ueno E, Minami M
  • Journal Title: Clin Radiol 62(3) Pages: 252-61
  • Peer Reviewed
• [Journal Article] WHO乳癌組織分類とわが国の乳癌取扱い規約分類の相違点 (2007)
  • Author(s): 菅間博, 寺戸雄一, 藤原正親
  • Journal Title: 臨床検査 51(1) Pages: 23-29
  • Peer Reviewed
• [Journal Article] 舌根部神経鞘腫例 (2007)
  • Author(s): 小柏靖直、甲能直幸、菅間博
  • Journal Title: 耳鼻咽喉科臨床 100(2) Pages: 84-85
  • NAID: 10018494741
  • Peer Reviewed
• [Journal Article] Growth suppression of thyroid cancer cells by adenylcyclase activator. (2007)
  • Author(s): Yano Y
  • Journal Title: Oncolgy Report Pages: 441-445
  • Peer Reviewed
• [Presentation] (杏林大学・医学部・病理) : スライドセミナー Case 5(乳腺) (2008)
  • Author(s): 菅間博, 水谷奈津子
  • Organizer: 第47回日本臨床細胞学会秋季大会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-15
• [Presentation] 過形成濾胞を包含する甲状腺髄様癌の一例 (2007)
  • Author(s): 菅間 博
  • Organizer: 日本甲状腺外科学会
  • Place of Presentation: 東京京王プラザホテル
  • Year and Date: 2007-10-19
• [Presentation] 過形成濾胞を包含する甲状腺髄様癌の一例混合性髄様・濾胞細胞癌"Hostage"仮説について (2007)
  • Author(s): 菅間博, 牛尾浩樹, 田中秀行, 深澤政勝, 松本裕文, 坂本穆彦
  • Organizer: 第40回日本甲状腺外科学会
  • Place of Presentation: 東京
• [Presentation] 腎盂癌に合併した微小パラガングリオーマの一例 (2007)
  • Author(s): 菅間博、藤原正親、原由紀子、坂本穆彦、木村伯子
  • Organizer: 第11回日本内分泌病理学会
  • Place of Presentation: 札幌
• [Book] 医学大辞典 代2版((伊藤・井村・高久 編)・(分担)「病理、細胞診」事項のアポトーシス他) (2008)
  • Author(s): 菅間博
  • Publisher: 医学書院、東京
• [Book] ルービンカラー病理学Q&A第21章内分泌系、第22章糖尿病、第23章アミロイドーシス (2007)
  • Author(s): 坂本穆彦 監訳
  • Publisher: 丸善, 東京