Analysis of the molecular mechanism in gastrointestinal pacemaker cell using KIT knock-in animal

• Project/Area Number: 19590411
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hyogo College of Medicine
• Principal Investigator: ISOZAKI Koji Hyogo College of Medicine, 医学部, 非常勤講師 (00425117)
• Co-Investigator(Kenkyū-buntansha): HIROTA Seiichi 兵庫医科大学, 医学部, 教授 (50218856)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 多発性GIST / ノックインマウス / カハールの介在細胞 / c-kit遺伝子 / 生殖系列 / 機能獲得性突然変異 / 遺伝子改変動物 / 消化管運動 / ペースメーカー / KIT / 多発製GIST / ノッタインマウス / exon 17 / exon17

Research Abstract

CADM1 is an adhesion molecule mediating nerve-mast cell interactions. It was demonstrated that both CADM1 protein and mRNA expressed in GISTs of human small intestine but not in GISTs of human stomach. These results suggested that types of ICCs which are considered to be an origin of GISTs are different between gastric and intestinal GISTs.

Research Products

• [Journal Article] in vivo effect of imatinib on progression of cecal GIST-like tumors in exon 17-type c-kit knock-in mice (2009)
  • Author(s): Isozaki K, Ishikawa T, Nakai K, Hirota S
  • Journal Title: Lab Invest 89 Pages: 1161-1168
  • Peer Reviewed
• [Journal Article] In vivo effect of imatinib on progression of cecal GIST-like tumors in exon 17-type c-kit knock-in mice (2009)
  • Author(s): Ishikawa T, Nakai N, Liu NN, Shiba K, Isozaki K, Matsuda I, Ito T, Fujimoto J, Hatakeyama K, Kanda T, Hirota S
  • Journal Title: Lab Invest 89 Pages: 1161-1168
  • Peer Reviewed
• [Journal Article] in vivo effect of imatinib on progression of cecal GIST-like tumors inexon 17-type c-kit knock-in mice (2009)
  • Author(s): Isozaki K, et al
  • Journal Title: Lab Invest 89 Pages: 1161-1168
  • Peer Reviewed
• [Journal Article] Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells. (2009)
  • Author(s): Sakurama K
  • Journal Title: Oncol Rep 21 Pages: 407-411
  • Peer Reviewed
• [Journal Article] Inhibition of focal adhesion kinase as a potential therapeutic strategy for Imatinib-resistant gastrointestinal stromal tumor. (2009)
  • Author(s): Sakurama K
  • Journal Title: Mol Cancer Ther 8 Pages: 127-134
  • Peer Reviewed
• [Journal Article] A mouse modele of a human multiple GIST family with KIT-Asp820Tyr mutation generated by a knock-in strategy (2008)
  • Author(s): Isozaki K, Nakai N, Hirota S
  • Journal Title: J Pathol 214 Pages: 302-311
  • Peer Reviewed
• [Journal Article] Abberant expression of glycosylation in juvenile gastrointestinal stromal tumors. (2008)
  • Author(s): Takahashi T
  • Journal Title: Proteomics Clin Appl 2 Pages: 1246-1254
  • Peer Reviewed
• [Journal Article] A mouse model of human multiple GIST family with KIT-Asp820Tyr mutation generated by knock-in strategy (2007)
  • Author(s): Nakai N
  • Journal Title: J Pathol 214 Pages: 302-311
  • Peer Reviewed
• [Journal Article] Characterization of tyrosine kinase I domain o-kit gene mutation Asn655Lys Newly found in primary jejunal gastrointestinal stromal tumor (2007)
  • Author(s): Kinoshita K
  • Journal Title: Am J Gastroenterol 102 Pages: 1134-1136
  • Peer Reviewed
• [Presentation] A mouse model of human GIST. (2008)
  • Author(s): Hirota S
  • Organizer: 2008 Korean GIST Multidisciplinary Symposium
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2008-11-29
• [Presentation] A mouse model of human multiple GIST family with KIT-Asp 820Tyr mutation generated by knock-in strategy. (2008)
  • Author(s): Nakai N
  • Organizer: DDW
  • Place of Presentation: San Diego, USA
  • Year and Date: 2008-05-19
• [Presentation] KIT-Asp820Tyr変異を持つ家族性多発性GIST家系のモデルマウスの作成 (2008)
  • Author(s): 伊藤敬
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Book] Bentham, Molecular mechanism and morphology in cancer (2010)
  • Author(s): Isozaki K, 他
  • Total Pages: 144
• [Book] Molecular mechanism and morphology in cancer (2010)
  • Author(s): Isozaki K, Hirota S.
  • Total Pages: 144
  • Publisher: Bentham