Microarray analysis of host gene-expression during intra-cellular nests formation of Leishmania amastigotes.

• Project/Area Number: 19590431
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Kumamoto University
• Principal Investigator: MIMORI Tatsuyuki Kumamoto University, 大学院・生命科学研究部, 教授 (00117384)
• Co-Investigator(Kenkyū-buntansha): KOGA Hisashi かずさDNA研究所, 室長 (30221237)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: マイクロアレイ / 感染症 / シグナル伝達 / 遺伝子 / 宿主細胞

Research Abstract

We observed invading amastigote of Leishmania amazonensisto macrophage host cells with microscope, and performed the temporary changes of the host cell with cDNA microarray. Total RNA was separated from the non-infected host cell which a mouse macrophage cell strain (J774A.1), infected cells in early stages of protozoan infection, in the infectious formation period, and parasites. Those samples were analyzed about 41,252 probes using the Oligo-microarray system (Agilent Technologies) (4 X 44K) 1 color method.