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Studies on the molecular mechanism of emesis and emetic receptor of staphylococcal enterotoxin

Research Project

Project/Area Number 19590438
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionHirosaki University

Principal Investigator

HU Dong-liang  Hirosaki University, 大学院・医学研究科, 准教授 (10333733)

Co-Investigator(Kenkyū-buntansha) NAKANE Akio  弘前大学, 大学院・医学研究科, 教授 (30164239)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords病原性 / エンテロトキシ / ブドウ球菌エンテロトキシン / スーパー抗原 / 嘔吐活性 / メカニズム / レセプター
Research Abstract

黄色ブドウ球菌の産生するエンテロトキシン(SE)は菌体外タンパク毒素で嘔吐誘導活性を示す一方,スーパー抗原活性を有することが知られている.しかし,SEによる食中毒のメカニズムはまだ明らかにされていない.本研究では, 小型嘔吐動物モデル・スンクスを用いSEAの催吐メカニズム及び細胞レセプターを検討した.これらの結果により, 小腸の5-HTの産生と放出,迷走神経求心性ニューロンの5-HT3 レセプターはSEAによる嘔吐反応に重要であり, またCB1レセプターは5-HTの産生と放出を抑制することによりSEAによる嘔吐反応を制御することが示唆された

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (53 results)

All 2009 2008 2007

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (35 results)

  • [Journal Article] Immunization with nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in house musk shrews2009

    • Author(s)
      Hu, D.-L., Omoe, K., Shinagawa, K. and Nakane, A.
    • Journal Title

      J.Infect.Dis 199(3)

      Pages: 302-310

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] D. K c-di-GMP as a vaccine adjuvant protects against systemic methicillin-resistant Staphylococcus aureus(MRSA) infection2009

    • Author(s)
      Hu, D.-L., Narita, K., Hyodo, M.,Hayakawa, Y., Nakane, A. and Karaolis
    • Journal Title

      Vaccine (In press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunization with nontoxic mutant staphylococcal enterotoxin A, D227 A, protects against enterotoxin-induced emesis in house musk shrews.2009

    • Author(s)
      Hu, D.-L., et.al.
    • Journal Title

      J. Infect. Dis. 199

      Pages: 301-310

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of intranasal vaccination with nontoxic mutant toxic shock syndrome toxin 1 against systemic and local Staphylo-coccusaureus infection2008

    • Author(s)
      Narita, K., Hu, D.-L., Tsuji, T. and Nakane, A.
    • Journal Title

      irosaki Med.J. 59(2)

      Pages: 71-82

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] ブドウ球菌エンテロトキシンの分子構造とその機能解析に関する研究2008

    • Author(s)
      胡東良
    • Journal Title

      弘前医学 59(2)

      Pages: 136-139

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intranasal immunization of mutant toxic shock syndrome toxin 1 elicit systemic and mucosal immune response against Staphylococcus aureus infection2008

    • Author(s)
      Narita, K., Hu, D.-L., Tsuji, T. and Nakane, A.
    • Journal Title

      FEMS Immun. Med. Microbiol 52(3)

      Pages: 389-396

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative prevalence of superantigenic toxin genes in methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates2008

    • Author(s)
      Hu, D.-L., Omoe, K., Inoue, F., Kasai,T., Yasujima, M., Shinagawa, K. and Nakane, A.
    • Journal Title

      J.Med.Microbiol 57(9)

      Pages: 1106-1112

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of two novel staphylococcal enterotoxins types Sand T.2008

    • Author(s)
      Ono, H., Omoe, K., Imanishi, K., Iwakabe, Y., Hu, D.-L., Kato, H., Saito, N., Nakane, A., Uchiyama, T. and Shinagawa, K.
    • Journal Title

      Infect.Immun 76(11)

      Pages: 4999-5005

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 黄色ブドウ球菌に対するワクチンの開発2008

    • Author(s)
      中根明夫, 成田浩司, 胡東良
    • Journal Title

      臨床免疫・アレルギー科 50(5)

      Pages: 543-550

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative Prevalence of Superantigenic Toxin Genes in Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates2008

    • Author(s)
      Hu, D. -L., et.al.
    • Journal Title

      J. Med. Microbiol 57

      Pages: 1106-1112

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of two novel staphylococcal enterotoxins types S and T2008

    • Author(s)
      Ono, H., et.al.
    • Journal Title

      Infect. Immun 76

      Pages: 4999-5005

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Staphylococcal enterotoxin induces emesis through increasing serotonin release inintestine and it is down-regulated by cannabinoid receptor 12007

    • Author(s)
      Hu, D.-L., Zhu, G., Mori, F., Omoe, K., Kaneko, S., Wakabayashi, K., Shinagawa, K. and Nakane, A.
    • Journal Title

      Cell. Microbiol 9(9)

      Pages: 2267-2277

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vaccination with a mutant of staphylococcal enterotoxin C elicits Th2 immune response for protection against Staphylococcus aureus infection2007

    • Author(s)
      Hu, D.-L., Omoe, K., Narita, K., Shinagawa, K., and Nakane, A.
    • Journal Title

      Hirosaki Med. J. 59(S)

      Pages: 210-218

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Staphylococcal enterotoxin A modulates intracellular Ca^<2+> signal pathway in human intestinal epithelial cells2007

    • Author(s)
      Hu, D.-L., Suga, Y., Omoe, K., Abe, Y., Shinagawa, K., Wakui, M. and Nakane, A.
    • Journal Title

      Hirosaki Med. J. 59(S)

      Pages: 219-226

    • NAID

      110006603001

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of intranasal vaccination with nontoxic mutant TSST-1 against Staphylococcus aureus infection2007

    • Author(s)
      Narita, K., Hu, D.-L., Tsuji, T. and Nakane, A.
    • Journal Title

      Hirosaki Med. J. 59(S)

      Pages: 227-234

    • NAID

      110006603003

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pollution of enteropatho-genic bacteria in meats and egg in Japan and China2007

    • Author(s)
      Hu, D.-L., Qian, A.-D., San, X.-F., Narita, K, Sashinami, H, Osanai, A., Abe, Y., Hiraga, Kudou, K., Liu, Y. and Nakane, A.
    • Journal Title

      Hirosaki Med. J. 59(1)

      Pages: 1-6

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Staphylococcal enterotoxin induces emesis through increasing serotonin release in intestine and it is down-regulated by cannabinoid receptor 1.2007

    • Author(s)
      Hu, D.-L., et. al.
    • Journal Title

      Cell. Microbiol. 9(9)

      Pages: 2267-2277

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pollution of enteropathogenic bacteria in meats and egg in Japan and China.2007

    • Author(s)
      Hu, D.-L., et. al.
    • Journal Title

      Hirosaki Med. J. 59

      Pages: 1-7

    • NAID

      110006573840

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] A nontoxic mutant staphylococcal enterotoxin A protects against enterotoxin-inducedemesis2009

    • Author(s)
      Hu, D.-L., Omoe, K., Sashinami, H., Shinagawa, K., Nakane, A.
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Immunization with nontoxic mutant staphylococcal enterotoxin A protects against enterotoxin-induced emesis in house musk shrews2009

    • Author(s)
      Hu, D.-L., Omoe, K., Sashinami, H., Shinagawa, K., Nakane, A.
    • Organizer
      The 11th Meeting Hirosaki International Forum of Medical Science
    • Place of Presentation
      Hirosaki, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] A nontoxic mutant staphylococcal enterotoxin A protects against enterotoxin-induced emesis2009

    • Author(s)
      Hu, D.-L., et.al.
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] ブドウ球菌エンテロトキシンAの腸管内動態2009

    • Author(s)
      小野久弥, ら
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] Immunization with nontoxic mutant staphylococcal enterotoxin A protects against enterotoxin-induced emesis in house musk shrews.2009

    • Author(s)
      Hu, D.-L., et.al.
    • Organizer
      The 11th Meeting Hirosaki International Forum of Medical Science
    • Place of Presentation
      Hirosaki, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] The role of interleukin-17 in the protective effect of an immunization with clumping factor A against Staphylococcus aureus infection2009

    • Author(s)
      Narita, K., et.al.
    • Organizer
      The 11th Meeting Hirosaki International Forum of Medical Science
    • Place of Presentation
      Hirosaki, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] 細菌超抗原毒素的分子多様性及生物多功能性的研究与応用2008

    • Author(s)
      胡東良, ら
    • Organizer
      中国農業科学院上海獣医研究所. 特別講演
    • Place of Presentation
      上海
    • Year and Date
      2008-12-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] MRSAとMSSA分離株のスーパー抗原毒素遺伝子の解析2008

    • Author(s)
      胡東良, 重茂克彦, 井上文緒, 葛西猛, 保嶋実, 品川邦汎, 中根明夫
    • Organizer
      第28回青森感染症研究会
    • Place of Presentation
      弘前
    • Year and Date
      2008-06-28
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ブドウ球菌エンテロトキシンの嘔吐誘導活性とスーパー抗原性の関連性2008

    • Author(s)
      胡 東良, ら
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] ブドウ球菌エンテロトキシンの分子構造とその機能解析に関する研究2008

    • Author(s)
      胡東良
    • Organizer
      第144回弘前医学会
    • Place of Presentation
      弘前
    • Year and Date
      2008-02-01
    • Related Report
      2008 Final Research Report
  • [Presentation] An immunization with fibrinogen binding domain of clumping factor A enhances IL-17 production and protects against Staphylococcus aureus infection(クランピングファクターAによる免疫はIL-17産生を促進し黄色ブドウ球菌感染防御効果を示す)2008

    • Author(s)
      Narita, K., Hu, D.-L., Nakane, A.
    • Organizer
      第38回日本免疫学会総会
    • Place of Presentation
      京都
    • Related Report
      2008 Final Research Report
  • [Presentation] Vaccination with nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in housemusk shrews2008

    • Author(s)
      Hu, D.-L., Omoe, K., Sashinami, H., Shinagawa, K., Nakane, A.
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] Staphylococcal enterotoxin A induces emesis through increasing serotonin release in intestine and endogenous cannabinoid system protects against the emetic response2008

    • Author(s)
      Hu, D.-L., Omoe, K., Shinagawa, K., Nakane, A.
    • Organizer
      13th International Symposium on Staphylococci & Staphylococcal Infections(ISSSI)
    • Place of Presentation
      Carens, Australia
    • Related Report
      2008 Final Research Report
  • [Presentation] ブドウ球菌エンテロトキシンの嘔吐誘導とその制御機序2008

    • Author(s)
      胡東良, 重茂克彦, 品川邦汎, 中根明夫
    • Organizer
      第2回日本細菌学・若手コロッセウム
    • Place of Presentation
      湘南
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 臨床由来のMRSAとMSSA分離株のブドウ球菌スーパー抗原毒素遺伝子の解析2008

    • Author(s)
      胡東良, 重茂克彦, 品川邦汎, 中根明夫
    • Organizer
      臨床由来のMRSAとMSSA分離株のブドウ球菌スーパー抗原毒素遺伝子の解析
    • Place of Presentation
      十和田
    • Related Report
      2008 Final Research Report
  • [Presentation] 黄色ブドウ球菌接着因子による感染予防効果2008

    • Author(s)
      成田浩司, 胡東良, 重茂克彦, 品川邦汎, 中根明夫
    • Organizer
      第62回日本細菌学会東北支部総会
    • Place of Presentation
      十和田
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 黄色ブドウ球菌接着因子による感染予防効果2008

    • Author(s)
      成田浩司, 胡東良, 重茂克彦, 品川邦汎, 中根明夫
    • Organizer
      第73回日本インターフェロン・サイトカイン学会学術集会/第19回日本生体防御学会学術総会/第45回補体シンポジウム合同学会
    • Place of Presentation
      札幌
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ブドウ球菌エンテロトキシンの嘔吐誘導活性とスーパー抗原活性の関連性2008

    • Author(s)
      胡東良, 重茂克彦, 品川邦汎, 中根明夫
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Related Report
      2008 Final Research Report
  • [Presentation] 黄色ブドウ球菌接着因子による感染予防効果2008

    • Author(s)
      成田浩司, 胡東良, 重茂克彦, 品川邦汎, 中根明夫
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Related Report
      2008 Final Research Report
  • [Presentation] 新規エンテロトキシンSESおよびSETの生物活性2008

    • Author(s)
      小野久弥, ら
    • Organizer
      第55回日本毒素シンポジウム
    • Place of Presentation
      山梨
    • Related Report
      2008 Annual Research Report
  • [Presentation] 臨床由来のMRSAとMSSA分離株のブドウスーパー抗原毒素遺伝子の解析2008

    • Author(s)
      胡東良, ら
    • Organizer
      第62回日本細菌学会東北支部総会
    • Place of Presentation
      十和田
    • Related Report
      2008 Annual Research Report
  • [Presentation] ブドウ球菌エンテロトキシン(SEs)の高感度検出法の確立と黄色ブドウ球菌株におけるSEs産生量評価2008

    • Author(s)
      稲垣華絵, ら
    • Organizer
      第62回日本細菌学会東北支部総会
    • Place of Presentation
      十和田
    • Related Report
      2008 Annual Research Report
  • [Presentation] ブドウ球菌エンテロトキシン遺伝子sesおよびsetの黄色ブドウ球菌集団における保有状況と存在様式の解明2008

    • Author(s)
      狩野真由子, ら
    • Organizer
      第62回日本細菌学会東北支部総会
    • Place of Presentation
      十和田
    • Related Report
      2008 Annual Research Report
  • [Presentation] Staphylococcal enterotoxin A induces emesis through increasing serotonin release in intestine and endogenous cannabinoid system protects against the emetic response2008

    • Author(s)
      Hu, D.-L., et.al.
    • Organizer
      13th International Symposium on Staphylococci & Staphylococcal Infections
    • Place of Presentation
      Carens, Australia
    • Related Report
      2008 Annual Research Report
  • [Presentation] New SEB-encoding Staphylococcus aureus pathogenicity island2008

    • Author(s)
      Omoe, K., et.al.
    • Organizer
      13th International Symposium on Staphylococci & Staphylococcal Infections
    • Place of Presentation
      Carens, Australia
    • Related Report
      2008 Annual Research Report
  • [Presentation] An immunization with fibrinogen binding domain of clumping factor A enhances IL-17 production znd protects against Staphylococcus aureus infection2008

    • Author(s)
      Narita, K., et.al.
    • Organizer
      第38回日本免疫学会総会
    • Place of Presentation
      京都
    • Related Report
      2008 Annual Research Report
  • [Presentation] ブドウ球菌エンテロトキシンB関連可動遺伝因子の多様性2008

    • Author(s)
      重茂克彦, ら
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] Vaccination with nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in house musk shrews2008

    • Author(s)
      Hu, D.-L., et.al.
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] 黄色ブドウ球菌エンテロトキシンファミリーの多様性とその機能2007

    • Author(s)
      胡東良
    • Organizer
      第12回弘前大学遺伝子実験施設シンポジウム
    • Place of Presentation
      弘前
    • Year and Date
      2007-11-16
    • Related Report
      2008 Final Research Report
  • [Presentation] 日本と中国における急性胃腸炎起因微生物の汚染調査2007

    • Author(s)
      胡東良, 銭愛東, 差波拓志, 単暁風, 成田浩司, 長内理大, 阿部由紀子, 平賀寛人, 工藤幸清, 劉勇, 中根明夫
    • Organizer
      青森感染症研究会
    • Place of Presentation
      青森
    • Year and Date
      2007-06-23
    • Related Report
      2008 Final Research Report
  • [Presentation] 黄色ブドウ球菌感染に対する無毒変異ワクチンの開発2007

    • Author(s)
      胡東良, 中根明夫
    • Organizer
      第91回弘前医学会総会
    • Place of Presentation
      大館
    • Year and Date
      2007-06-09
    • Related Report
      2008 Final Research Report
  • [Presentation] 黄色ブドウ球菌局所感染および全身感染に対する無毒変異TSST-1経鼻粘膜ワクチンの防御効果2007

    • Author(s)
      成田浩司, 胡 東良, 辻孝雄, 中根明夫
    • Organizer
      第37回日本免疫学会総会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] ブドウ球菌エンテロトキシンの嘔吐誘導機序2007

    • Author(s)
      胡東良, 重茂克彦, 品川邦汎, 中根明夫
    • Organizer
      第54回日本毒素シンポジウム
    • Place of Presentation
      大阪
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] ブドウ球菌エンテロトキシンAの嘔吐誘導とその制御機序2007

    • Author(s)
      胡東良, 重茂克彦, 品川邦汎, 中根明夫
    • Organizer
      第61回日本細菌学会東北支部総会
    • Place of Presentation
      仙台
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 無毒変異TSST-1経鼻粘膜ワクチンによる黄色ブドウ球菌感染防御効果2007

    • Author(s)
      成田浩司, 胡東良, 辻孝雄, 中根明夫
    • Organizer
      第72回日本インターフェロン・サイトカイン学会
    • Place of Presentation
      京都
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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