Analysis of secretion system of virulence factor in gram-negative bacteria

• Project/Area Number: 19590440
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Chiba University
• Principal Investigator: SHIMIZU Takeshi Chiba University, 大学院・医学研究院, 講師 (70312840)
• Co-Investigator(Kenkyū-buntansha): 野田 公俊 千葉大学, 大学院・医学研究院, 教授 (60164703) ; 盛永 直子 千葉大学, 大学院・医学研究院, 准教授 (20092108)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 感染症 / 細菌 / 分泌 / 細菌毒素 / 最近 / 腸管出血性大腸菌 / 志賀様毒素

Research Abstract

腸管出血性大腸菌の産生する志賀毒素2(Stx2)は2種類の方法によって菌体内から、特異的に放出されることが明らかになった。一つ目の機構はstx2遺伝子が存在しているStx2ファージが特異的にファージ誘導されることによって、同調的にStx2が産生され、そのファージの容菌によってStx2も菌体外に特異的に放出される。二つ目はStx2特異的な分泌機構が存在しており、この分泌によって、Stx2が特異的に菌体外に輸送される。この過程にはStx2のBサブユニットの31番目のセリン残基が重要であった。

Research Products

• [Journal Article] Shiga toxin 2 is specifically released from bacterial cells by two different mechanisms (2009)
  • Author(s): Takeshi Shimizu, Yuko Ohta and Masatoshi Noda
  • Journal Title: Infect.Immun. (in press)
  • Peer Reviewed
• [Journal Article] Differential entry of Botulinum neurotoxin A into neural and intestinal cells (2009)
  • Author(s): Aurelie Couesnon, Takeshi Shimizu and Michel R. Popoff
  • Journal Title: Cell. Microbiol. 11 Pages: 289-308
  • Peer Reviewed
• [Journal Article] Differential entry of botulinum neurotoxin A into neural and intestinal cells. (2009)
  • Author(s): Aurelic Couesnon
  • Journal Title: Cell. Microbiol. 11 Pages: 289-308
  • Peer Reviewed
• [Journal Article] A nasal vaccine comprising B-subunit derivative of Shiga toxin 2 for cross-protection aganst Shiga toxin types 1 and 2 (2008)
  • Author(s): Takao Tsuji, Takeshi Shimizu, Keiko Sasaki, Kentaro Tsukamoto, Hideyuki Arimitsu, Sadayuki Ochi, Toshiyasu Shimizu, Koki Taniguchi, Masatoshi Noda, Paola Neri, Hiroshi Mori
  • Journal Title: Vaccine 26 Pages: 2092-2099
  • Peer Reviewed
• [Journal Article] Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin (2008)
  • Author(s): Takao Tsuji, Takeshi Shimizu, Keiko Sasaki, Toshiyasu Shimizu, Kentaro Tsukamoto, Hideyuki Arimitsu, Sadayuki Ochi, Satoshi Sugiyama, Koki Taniguchi, Paola Neri, Hiroshi Mori
  • Journal Title: Vaccine 26 Pages: 469-476
  • Peer Reviewed
• [Journal Article] Subtilase cytotoxin, produced by Shiga-toxigenic Escherichia coli, transiently inhibits protein synthesis of Vero cells via degradation of BiP and induces cell cycle arrest at G1 by downregulation of cyclin D1 (2008)
  • Author(s): Naoko Morinaga, Kinnosuke Yahiro, Gen Matsuura, Joel Moss and Masatoshi Noda
  • Journal Title: Cellular Microbiology 10 Pages: 921-929
  • Peer Reviewed
• [Journal Article] Effect of Gb3 in lipid rafts in resistance to Shiga-like toxin of mutant Vero cells (2008)
  • Author(s): Tatsuo Hanashima, Masami Miyake, Kinnosuke Yahiro, Yoshifumi Iwamaru, Akikazu Ando, Naoko Morinaga and Masatoshi Noda
  • Journal Title: Microb. Pathog. 45 Pages: 124-133
  • Peer Reviewed
• [Journal Article] A nasal vaccine comprising B-subunit derivative of Shiga toxin 2 for cross-protection aganst Shiga toxin types 1 and 2. (2008)
  • Author(s): Takao Tsuji
  • Journal Title: Vaccine 26 Pages: 2092-2099
  • Peer Reviewed
• [Journal Article] Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escheichia coli enterotoxin (2008)
  • Author(s): Takao Tsuji
  • Journal Title: Vaccine 26 Pages: 469-476
  • Peer Reviewed
• [Journal Article] Receptor affinity, stability and binding mode of Shiga toxins are determinants of toxicity (2007)
  • Author(s): Takeshi Shimizu, Toshio Sato, Satomi Kawakami, Toshiko Ohta, Masatoshi Noda and Takashi Hamabata
  • Journal Title: Microb. Pathog. 43 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Serine 31 residue of B subunit of Shiga toxin 2 is essential for the secretion in Enterohemorrhagic Escherichia coli (2007)
  • Author(s): Takeshi Shimizu, Satomi Kawakami, Toshio Sato, Terumi Sasaki, Masato Higashide, Takashi Hamabata, Toshiko Ohta and Masatoshi Noda
  • Journal Title: Infect. Immun. 75 Pages: 2189-2200
  • Peer Reviewed
• [Journal Article] Serine 31 residue of B subunit of Shiga toxin 2 is essential for the secretion in Enterohemorrhagic Escherichia coli (2007)
  • Author(s): Takeshi Shimizu
  • Journal Title: Infect. Immun 75 Pages: 2189-2200
  • Peer Reviewed
• [Journal Article] Receptor affinity, stability and binding mode of Shliga toxins are determinants of toxicity (2007)
  • Author(s): Takeshi Shimizu
  • Journal Title: Microb. Pathog 43 Pages: 88-95
  • Peer Reviewed
• [Presentation] 腸管出血性大腸菌には志賀毒素2に対する2種類の特異的な放出機構がある (2009)
  • Author(s): 清水健、野田公俊
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋、名古屋国際会議場
• [Presentation] 腸管出血性大腸菌には志賀毒素2に対する2種類の特異的な放出機構がある (2009)
  • Author(s): 清水健
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋, 名古屋国際会議場
• [Presentation] 志賀様毒素2の分泌にはBサブユニットの特定のアミノ酸残基の側鎖が重要である (2008)
  • Author(s): 清水 健
  • Organizer: 日本細菌学会
  • Place of Presentation: 国際会議場・京都
  • Year and Date: 2008-03-24
• [Presentation] A mechanism of Stx2 release from enterohemorrahgic Escherichia coli by a non-secretion system (2008)
  • Author(s): Takeshi Shimizu, Yuko Ohta and Masatoshi Noda
  • Organizer: 43^<rd> Joint Conference on Cholera and Other Bacterial Enteric Infections Panel
  • Place of Presentation: Centennial Hall in Kyushu University, Fukuoka
• [Presentation] 志賀毒素2の分泌にはBサブユニットの特定のアミノ酸残基の側鎖が重要である (2008)
  • Author(s): 清水健、佐藤壽男、濱端崇、野田公俊
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都、国立京都国際会館
• [Presentation] Binding mode of chimera Shiga toxins and their toxicity (2007)
  • Author(s): Takeshi Shimizu, Toshio Sato, Takashi Hamabata and Masatoshi Noda
  • Organizer: 42^<nd> Joint Conference on Cholera and Other Bacterial Enteric Infections Panel
  • Place of Presentation: Austin, Texas
• [Presentation] Binding mode of chimera Shiga toxins and their toxicity (2007)
  • Author(s): Takeshi Shimizu
  • Organizer: 42ndJoint Conference on Cholera and Other Bacterial Enteric Infections Panel
  • Place of Presentation: Austin,Texas