Pathophysiological analysis of the emesis induced by Staphyrococcal enterotoxin

• Project/Area Number: 19590444
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: SUGIMOTO Nakaba Osaka University, 大学院・医学系研究科, 教授 (20142317)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 感染免疫 / 食中毒 / ブドウ球菌 / エンテロトキシン / 消化管蠕動運動 / パターンジェネレーター / 嘔吐運動

Research Abstract

Mouse small intestine twitch in response to electrical field stimulation. The response were composed of two peaks, a fast peak which appears after ca. 1 sec and the second sloe peak which appears after ca. 2.5 sec. The amplitude of the second peak increased remarkably after the treatment of the intestinal preparation with staphyrococcal eneterotoxin A. Monoamine seemed to mediate the second peak but CPG activity in CNS of the crayfish which is reported to be mediated by monoamines showed no obvious change by the enterotoxin.

Research Products

• [Journal Article] Comparison of effects of botulinum toxin subtyoe A1 and A2 using twitch tension assay and rat grip strength test. (2011)
  • Author(s): Torii, Y., N. Kiyota, N. Sugimoto, Y. Mori, Y. Goto, T. Harakawa, S. Nakahira, R. Kaji, S. Kozaki, A. Ginnaga
  • Journal Title: Toxicon 57 Pages: 93-99
• [Journal Article] Comparison of effects of botuhnum toxin subtyoe A1 and A2 using twitch tension assay and rat grip strength test. (2011)
  • Author(s): 鳥居恭司、杉本央
  • Journal Title: Toxicon Volume: 57 Pages: 93-99
  • Peer Reviewed
• [Journal Article] PCR with quenching probes enables the rapid detection and identification of ganciclovir-resistance causing U69 gene mutations in human herpesvirus 6. (2010)
  • Author(s): Isegawa, Y., C. Matsumoto, K. Nishinaka, K. Nakano, N. Sugimoto, A. Oshima
  • Journal Title: Molecular and Cellular Probes 24 Pages: 167-177
• [Journal Article] Activation of motility by sensing short-chain fatty acids via two steps in a glagellar gene regulatory cascade in enterohemorrhagic Escherichia coli. (2010)
  • Author(s): Tobe, T., N. Nakanishi, N. Sugimoto
  • Journal Title: Infection and Immunity 79 Pages: 1016-1024
• [Journal Article] NleC, a type III secretion protease, compromises NF-kB activation by targeting p65/RelA. (2010)
  • Author(s): Yen, H., T. Ooka, A. Iguchi, T, Hayashi, N. Sugimoto, T. Tobe
  • Journal Title: PloS athogens 6(12)
• [Journal Article] NIeC, a type III secretion protease, compromises NF-kB activation by targeting p65/RelA. (2010)
  • Author(s): 顔宏哲、杉本央
  • Journal Title: PloS Pathogens Volume: 6
  • Peer Reviewed
• [Journal Article] Activation of motility by sensing short chain fatty acids via two stepsin a glagellar gene regulatory cascade in enterohemorrhagic Escherichiacoli. (2010)
  • Author(s): 戸邉亨、杉本央
  • Journal Title: Infection and Immunity Volume: 79 Pages: 1010-1020
  • Peer Reviewed
• [Journal Article] PCR with quenching probes enables the rapid detection and identification of ganciclovir-resistance causing U69 gene mutations in human herpesvirus 6. (2010)
  • Author(s): 伊勢川裕二、杉本央
  • Journal Title: Molecular and Cellular Probes Volume: 24 Pages: 167-177
  • Peer Reviewed
• [Journal Article] Detection and identification of U69 gene mutations encoded by ganciclovir-resistant human herpesvirus 6 using denaturing high-performance liquid chromatography. (2009)
  • Author(s): Kazushi Nakano, Kazuko Nishinaka, Tatsuya Tanaka, Atsushi Ohshima, Nakaba Sugimoto, Yuji Isegawa
  • Journal Title: Journal of Virological Methods 161 Pages: 223-230
• [Journal Article] Enterohemorrhagic Escherichia coli effector EspL2 induces actin microfilament aggregation through annexin 2 activation. (2009)
  • Author(s): A. Miyahara, N. Nakanishi, T. Ooka, T. Hayashi, N. Sugimoto, T. Tobe
  • Journal Title: Cellular Microbiology 11, 2 Pages: 337-350
• [Journal Article] Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. (2009)
  • Author(s): N. Nakanishi, K. Tashiro, S. Kuhara, T. Hayashi, N. Sugimoto, T. Tobe
  • Journal Title: Microbiology 155, 2 Pages: 521-530
• [Journal Article] Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli (2009)
  • Author(s): N. Nakanishi, K. Tashiro, S. Kuhara, T. Hayashi, N. Sugimoto, T. Tobe
  • Journal Title: Microbiology 155, 2 Pages: 521-530
  • Peer Reviewed
• [Journal Article] Enterohemorrhagic Escherichia coli effector EspL2 induces actin microfilament aggregation through annexin 2 activation (2009)
  • Author(s): A. Miyahara, N. Nakanishi, T. Ooka, T. Hayashi, N. Sugimoto, T. Tobe
  • Journal Title: Cellular Microbiology 11, 2 Pages: 337-350
  • Peer Reviewed
• [Journal Article] Detection and identification of U69 gene mutations encoded by ganciclovir-resistant human herpesvirus 6 using denaturing high-performance liquid chromatography (2009)
  • Author(s): Kazushi Nakano, Kazuko Nishinaka, Tatsuya Tanaka, Atsushi Ohshima, Nakaba Sugimoto, Yuji Isegawa
  • Journal Title: Journal of Virological Methods 161 Pages: 223-230
  • Peer Reviewed
• [Journal Article] Regulation of virulence by butyrate sensing in entero-haemorrhagic Escherichia coli (2009)
  • Author(s): N.Nakanishi, K.Tashiro, S.Kuhara, T.Hayashi, N.Sugimoto, T.Tobe
  • Journal Title: Microbiology 155 Pages: 521-530
  • Peer Reviewed
• [Journal Article] Enterohemorrhagic Escherichia coli effector EspL2 induces actin microfilament aggregation through annexin 2 activation (2009)
  • Author(s): A.Miyahara, N.Nakanishi, T.Ooka, T.Hayashi, N.Sugimoto, T.Tobe
  • Journal Title: Cellular Microbiology 11 Pages: 337-350
  • Peer Reviewed
• [Journal Article] Relationship between U83 gene variation in human herpes-virus 6 and secretion of the U83 gene product. (2009)
  • Author(s): Sjahril, R. ら
  • Journal Title: Archives of Virology 154 Pages: 273-283
  • Peer Reviewed
• [Journal Article] Regulation of virulence and motility by butyrate sensing in enterohemorrhagic Escherichia coli. (2009)
  • Author(s): Nakanishi, N. ら
  • Journal Title: Microbiology 155 Pages: 521-530
  • Peer Reviewed
• [Journal Article] Characterization of the human herpesuvirus 6 U69 gene product and identification of its nuclear localization signal. (2008)
  • Author(s): Yuji Isegawa, Yoichi Miyamoto, Yoshinari Yasuda, Katsunori Semi, Kenji Tsujimura, Rikiro Fukunaga, Atsushi Ohshima, Yasuhiro Horiguchi, Yoshihiro Yoneda, Nakaba Sugimoto
  • Journal Title: Journal of Virology 82, No.2 Pages: 710-718
• [Journal Article] Characterization of the human herpesuvirus 6 U69 gene product and identification of its nuclear localization signal (2008)
  • Author(s): Yuji Isegawa, Yoichi Miyamoto, Yoshinari Yasuda, Katsunori Semi, Kenji Tsujimura, Rikiro Fukunaga, Atsushi Ohshima, Yasuhiro Horiguchi, Yoshihiro Yoneda, Nakaba Sugimoto
  • Journal Title: Journal of Virology 82, No. 2 Pages: 710-718
  • Peer Reviewed
• [Journal Article] Characterization of the human herpesuvirus 6 U69 gene product and identification of its nuclear localization signal. (2008)
  • Author(s): Isegawa, Y. ら
  • Journal Title: Journal of Virology 82 Pages: 710-718
  • Peer Reviewed
• [Journal Article] Reactivation of latent human immunodeficiency virus 1 by human herpesvirus 6 infection. (2007)
  • Author(s): Y. Isegawa, J. Katahira, K. Yamanishi, N. Sugimoto
  • Journal Title: Acta Virologica 51 Pages: 13-20
• [Journal Article] Maturation of functional type III secretion machinery by activation of anaerobic respiration in enterohaemorrhagic Escherichia coli. (2007)
  • Author(s): Hiroki Ando, Hiroyuki Abe, N.Sugimoto, Toru Tobe
  • Journal Title: Microbiology 153 Pages: 464-473
• [Journal Article] Real-time PCR determination of human herpes virus 6 antiviral drug susceptibility. (2007)
  • Author(s): Yuji Isegawa, Masaya Takemoto, Koichi Yamanishi, Atsushi Ohshima, Nakaba Sugimoto
  • Journal Title: Journal of Virological Methods 140 Pages: 25-31
• [Journal Article] Maturation of functional type III secretion machinery by activation of anaerobic respiration in enterohaemorrhagic Escherichia coli (2007)
  • Author(s): Hiroki Ando, Hiroyuki Abe, Nakaba Sugimoto, Toru Tobe
  • Journal Title: Microbiology 153 Pages: 464-473
  • Peer Reviewed
• [Journal Article] Maturation of functional type III secretion machinery by activation of anaerobic respiration in enterohaemorrhagic Escherichia coli. (2007)
  • Author(s): Hiroki Ando, ら
  • Journal Title: Microbiology 153 Pages: 464-473
  • Peer Reviewed
• [Journal Article] Real-time PCR determination of human herpes virus 6 antiviral drug susceptibility. (2007)
  • Author(s): Yuji Isegawa, ら
  • Journal Title: Journal of Virological Methods 140 Pages: 25-31
  • Peer Reviewed
• [Journal Article] Reactivation of latent human in immunodeficiency virus 1 by human herpesvirus 6 infection. (2007)
  • Author(s): Y. Isagawa, ら
  • Journal Title: Acta Virologica 51 Pages: 13-20
  • Peer Reviewed