Biochemical analysis of the effect of Chlamydia pneumoniae infectionon miRNA expression and host cellular functions.

• Project/Area Number: 19590447
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: HIRAI Itaru Osaka University, 大学院・医学系研究科, 准教授 (00359994)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Yoshimasa 大阪大学, 大学院・医学系研究科, 教授 (20010100)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 微生物 / シグナル伝達 / 遺伝子

Research Abstract

本研究では、リンパ球への肺炎クラミジア感染に伴う宿主細胞のシグナル分子やアポトーシスに関与する分子の変動など及びマイクロRNA(miRNA)発現の変動の点に着目しながら、クラミジア感染の宿主細胞への影響について解明することを目的とした.その結果クラミジア感染により宿主細胞の増殖が抑制され, 宿主細胞のmiRNA-214 の発現量の増加が観察された.肺炎クラミジア感染によるcyclinD3 の発現抑制を介する細胞増殖の調節においては, mTOR 経路の関与が最も疑われた結果が得られた

Research Products

• [Journal Article] Analysis ofHelicobacter pylori genotype in stoolspecimens of asymptomatic people. (2009)
  • Author(s): Sasaki T, Hirai I, Izurieta R, Kwa BH, Estevez E , Saldana A, Calzada JE, Fujimoto S, Yamamoto Y
  • Journal Title: Labmedicine (accepted)
  • Peer Reviewed
• [Journal Article] A method for assessment of Helicobacter pylorigenotype using stool specimens (2009)
  • Author(s): Hirai I, Sasaki T, Fujimoto S, Moriyama T, Azuma T, Yamamoto Y
  • Journal Title: FEMS Immunology & Medical Microbiology 56(1) Pages: 63-66
  • Peer Reviewed
• [Journal Article] 便中抗原検出及びPCR 法を用いたパナマ健常人におけるHelicobacter pylori感染の解析 (2009)
  • Author(s): 佐々木正大, 平井到, 山本容正
  • Journal Title: 感染症学雑誌 83(2) Pages: 127-132
  • NAID: 10024803517
  • Peer Reviewed
• [Journal Article] 食品由来ポリフェノールによるLegionella pneumophila 増殖抑制活性 (2009)
  • Author(s): 奥野真奈, 平井到, 佐々木正大, 山本容正
  • Journal Title: 医学と生物学 153(1) Pages: 1-6
  • NAID: 40016435971
  • Peer Reviewed
• [Journal Article] Immunosuppressive effect on T cell activation by interleukin-16- andinterleukin-10-cDNA-double-transfected human squamous cell line. (2009)
  • Author(s): Matsumoto Y, Fujita T, Hirai I, Sahara H, Torigoe T, Ezoe K, Saito T, Cruikshank WW, Yotsuyanagi T, Sato N
  • Journal Title: Burns 35(3) Pages: 383-389
  • Peer Reviewed
• [Journal Article] Analysis of Helicobacter pylori genotype in stool specimens of asymptomatic people. (2009)
  • Author(s): Tadahiro Sasaki
  • Journal Title: Lab Medicine (印刷中)
  • Peer Reviewed
• [Journal Article] A method for assessment of Helicobacter pylori genotype using stool specimens (2009)
  • Author(s): Itaru Hirai
  • Journal Title: FEMS Immunology and Medical Microbiology (印刷中)
  • Peer Reviewed
• [Journal Article] Immunosuppressive effect on T cell activation by interleukin-16- and interleukin-10-cDNA-double-transfected human squamous cell line (2009)
  • Author(s): Yoshitaka Matsumoto
  • Journal Title: Burns (印刷中)
  • Peer Reviewed
• [Journal Article] 食品由来ポリフェノールによる Legionella pneumophila増殖抑制活性 (2009)
  • Author(s): 奥野真奈
  • Journal Title: 医学と生物学 153 Pages: 1-6
• [Journal Article] 健常人保菌Helicobacter pylori の検出と病原因子遺伝子解析 (2008)
  • Author(s): 佐々木正大, 平井 到, Richard Izurieta, Boo Kwa, 山本容正
  • Journal Title: 臨床と微生物 35(5) Pages: 475-480
  • Peer Reviewed
• [Journal Article] Disruption ofthe association of 73 kDa heat shockcognate protein with transportersassociated with antigen processing (TAP)decreases TAP-dependent translocationof antigenic peptides into theendoplasmic reticulum (2008)
  • Author(s): Kamiguchi K, Torigoe T, Fujiwara O, Ohshima S, Hirohashi Y, Sahara H, Hirai I, Kohgo Y, Sato N
  • Journal Title: Microbiol Immunol 52(2) Pages: 94-106
  • Peer Reviewed
• [Journal Article] 健常人保菌Helicobacter pyloriの検出と病原因子遺伝子解析 (2008)
  • Author(s): 佐々木正大
  • Journal Title: 臨床と微生物 35 Pages: 475-480
  • Peer Reviewed
• [Presentation] Chlamydia pneumoniae感染によるヒト白血病由来細胞株Jurkat 細胞の増殖抑制機序の解明 (2009)
  • Author(s): 榎原愛
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-03-13
• [Presentation] Chlamydia pneumoniae感染によるヒト白血病由来細胞株Jurkat細胞の増殖抑制機序の解明 (2009)
  • Author(s): 榎原 愛
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-03-13
• [Presentation] Chlamydia pneumoniae感染による宿主リンパ球細胞周期関連蛋白CyclinD3 の発現調節 (2008)
  • Author(s): 榎原愛
  • Organizer: 第61回日本細菌学会関西支部総会
  • Place of Presentation: 京都市
  • Year and Date: 2008-11-08
• [Presentation] Chlamydia pneumoniae感染によるmiRNA 発現の解析 (2008)
  • Author(s): 田中香苗
  • Organizer: 第61回日本細菌学会関西支部総会
  • Place of Presentation: 京都市
  • Year and Date: 2008-11-08
• [Presentation] Chlamydia pneumoniae感染による宿主リンパ球細胞周期関連蛋白CyclinD3の発現調節 (2008)
  • Author(s): 榎原 愛
  • Organizer: 第61回日本細菌学会関西支部総会
  • Place of Presentation: 京都市
  • Year and Date: 2008-11-08
• [Presentation] Chlamydia pneumoniae感染によるmiRNA発現の解析 (2008)
  • Author(s): 田中香苗
  • Organizer: 第61回日本細菌学会関西支部総会
  • Place of Presentation: 京都市
  • Year and Date: 2008-11-08
• [Presentation] Chlamydia pneumoniae感染宿主リンパ球細胞増殖抑制機構の解明 (2008)
  • Author(s): 榎原愛
  • Organizer: 第26回日本クラミジア研究会
  • Place of Presentation: 岐阜市
  • Year and Date: 2008-11-01
• [Presentation] Genotyping of virulencegene cagA of Helicobacter pylori in stool specimens from asymptomatic volunteeres in Japan and Thailand (2008)
  • Author(s): Itaru Hirai
  • Organizer: 48thAnnual Interscience Conference onAntimicrobial Agents and Chemotherapy(ICAAC)/ the Infectious DiseasesSociety of America (IDSA) 46th AnnualMeeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-10-26
• [Presentation] Genotyping of virulence gene cagA of Helicobacter pylori in stool specimens from asymptomatic volunteeres in Japan and Thailand. (2008)
  • Author(s): Itaru Hirai
  • Organizer: 48th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) / the Infectious Diseases Society of America (IDSA) 46th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-10-26
• [Presentation] 偏性細胞内寄生性細菌感染に起因する宿主細胞miRNA 発現の測定 (2008)
  • Author(s): 田中香苗
  • Organizer: 第48回近畿医学検査学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-10-18
• [Presentation] Chlamydia pneumoniae感染による宿主リンパ球細胞周期関連蛋白CyclinD3 の発現抑制 (2008)
  • Author(s): 榎原愛
  • Organizer: 第48回近畿医学検査学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-10-18
• [Presentation] 偏性細胞内寄生性細菌感染に起因する宿主細胞miRNA発現の測定 (2008)
  • Author(s): 田中香苗
  • Organizer: 第48回近畿医学検査学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-10-18
• [Presentation] Chlamydia pneumoniae感染による宿主リンパ球細胞周期関連蛋白CyclinD3の発現抑制 (2008)
  • Author(s): 榎原 愛
  • Organizer: 第48回近畿医学検査学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-10-18
• [Presentation] Epidemiological studyof Helicobacter pylori infection in Thaiand Japanese healthy people bycoprodiagnosis (2008)
  • Author(s): Tadahiro Sasaki
  • Organizer: The Forum of theNetwork of Research Centers onInfectious Diseases
  • Place of Presentation: Hanoi, Vietnam
  • Year and Date: 2008-10-06
• [Presentation] Epidemiological study of Helicobacter pylori infection in Thai and Japanese healthy people by coprodiagnosis. (2008)
  • Author(s): Tadahiro Sasaki
  • Organizer: The Forum of the Network of Research Centers on Infectious Diseases
  • Place of Presentation: Hanoi, Vietnam
  • Year and Date: 2008-10-06
• [Presentation] Analysis of Helicobacter pylori virulence factorgenotypes and intestinal parasites infecal specimens of healthy subjects. (2008)
  • Author(s): Tadahiro Sasaki
  • Organizer: 108th The American Society for Microbiology General Meeting
  • Place of Presentation: Boston, USA
• [Presentation] Analysis of Helicobacter pylori virulence factor genotypes and intestinal parasites in fecal spec imens of healthy subjects. (2008)
  • Author(s): Tadahiro Sasaki
  • Organizer: 108^<th> The American Society for Microbiology General Meeting
  • Place of Presentation: Boston, USA
• [Presentation] Chlamydia pneumoniae 感染による宿主細胞の変化の解析 アポトーシスとmiRNA の発現変化 (2007)
  • Author(s): 和田沙織
  • Organizer: 第50回 日本臨床検査医学会近畿支部総会
  • Place of Presentation: 大阪市
  • Year and Date: 2007-11-24
• [Presentation] Chlamydia pneumoniae感染による宿主細胞の変化の解析 アポトーシスとmiRNAの発現変化 (2007)
  • Author(s): 和田沙織、田中香苗、西村典孝、平井 到、山本容正
  • Organizer: 第50回 日本臨床検査医学会近畿支部総会
  • Place of Presentation: 大阪府大阪市大阪国際会議場
  • Year and Date: 2007-11-24
• [Presentation] Chlamydia pneumoniae 感染による宿主細胞アポトーシスとmiRNA 発現変化 (2007)
  • Author(s): 和田沙織
  • Organizer: 第60回日本細菌学会関西支部総会
  • Place of Presentation: 吹田市
  • Year and Date: 2007-11-10
• [Presentation] Chlamydia pneumoniae感染による宿主細胞アポトーシスとmiRNA発現変化 (2007)
  • Author(s): 和田沙織、田中香苗、平井 到、山本容正
  • Organizer: 第60回日本細菌学会関西支部総会
  • Place of Presentation: 大阪府吹田市大阪大学銀杏会館
  • Year and Date: 2007-11-10