Modified mycolic acids in Mycobacterium tuberculosis regulate the host immune responses

• Project/Area Number: 19590453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka City University
• Principal Investigator: FUJIWARA Nagatoshi Osaka City University, 大学院・医学研究科, 講師 (80326256)
• Co-Investigator(Kenkyū-buntansha): YANO Ikuya 日本ビーシージー製造株式会社, 中央研究所, 顧問 (60047008)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 感染免疫 / 脂質分子 / 宿主応答 / 結核菌 / ミコール酸 / 感染症 / 細菌 / 脂質 / 生理活性 / 免疫学 / 生活活性

Research Abstract

Mycolic acid is a characteristic cell wall component expressed in acid-fast bacteria containing Mycobacterium tuberculosis. The heterogeneity of mycolic acid subclasses (α, methoxy, keto, dicarboxy, epoxy, etc), unsaturations (monoene, diene, cyclopropane), and geometrical isomers (cis, trans) effected to cord forming, acid-fastness, and host immune responses to Mycobacteria. The modifications of mycolic acids play an important role in their virulence and pathogenecity.

Research Products

• [Journal Article] Identification of Rhodococcus equi lipids recognized by host cytotoxic T-lymphocytes. (2010)
  • Author(s): S.P. Harris, N. Fujiwara, R.H. Mealey, D.C. Alperin, T. Naka, R. Goda, S.A. Hines
  • Journal Title: Microbiology (in press)
  • Peer Reviewed
• [Journal Article] Identification of Rhodococcus equi lipids recognized by host cytotoxic T-lymphocytes. (2010)
  • Author(s): Harris, S.P.
  • Journal Title: Microbiology (In press)
  • Peer Reviewed
• [Journal Article] Mycobacterium kyorinense sp. nov., a novel slowly growing Mycobacterium sp. related to Mycobacterium celatum isolated from human clinical specimens. (2009)
  • Author(s): M. Okazaki, K. Ohkusu, H. Hata, H. Ohnishi, K. Sugawara, C. Kawamura, N. Fujiwara, S. Matsumoto, Y. Nishiuchi, K. Toyoda, H. Saito, S. Yonetani, Y. Fukugawa, M. Yamamoto, H. Wada, A. Sejimo, A. Ebina, H. Goto, T. Ezaki, T. Watanabe
  • Journal Title: IJSEM 59 Pages: 1336-1341
  • Peer Reviewed
• [Journal Article] Comparable Studies of Immunostimulating Activities In Vitro among Mycobacterium bovis bacillus Calmette-Guerin (BCG) Sub-strains. (2009)
  • Author(s): D. Hayashi, T. Takii, N. Fujiwara, Y. Fujita, I. Yano, S. Yamamoto, M. Kondo, E. Yasuda, E. Inagaki, K. Kanai, A. Fujiwara, A. Kawarazaki, T. Chiba, K. Onozaki
  • Journal Title: FEMS Immunol. Med. Microbiol 56 Pages: 116-128
  • Peer Reviewed
• [Journal Article] ″Lysobacter enzymogenes subsp. cookii″Christensen 1 1978 should be recognized as an independent species Lysobacter cookii sp. nov. (2009)
  • Author(s): Y. Kawamura, J. Tomida, Y. Morita, T. Naka, S. Mizuno, N. Fujiwara
  • Journal Title: FEMS Microbiol. Lett 298 Pages: 118-23
  • Peer Reviewed
• [Journal Article] Mycobacterium kyorinense sp. Nov., a novel slowly growing Mycobacterium sp.related to Mycobacterium celatum isolated from human clinical specimens. (2009)
  • Author(s): Okazaki, M.
  • Journal Title: Int. J.Syst. Evol. Microbiol. 59 Pages: 1336-1341
  • Peer Reviewed
• [Journal Article] Comparable studies of immunostimulating activities in vitro among Mycobacterium bovis bacillus Calmette-Guerin (BCG) sub-strains. (2009)
  • Author(s): Hayashi, D.
  • Journal Title: FEMS Immunol. Med. Microbiol. 56 Pages: 116-128
  • Peer Reviewed
• [Journal Article] "Lysobacter enzymogenes subsp. cookii" Christensen 1 1978 should be recognized as an independent species Lysobacter cookii sp. nov. (2009)
  • Author(s): kawamura, Y.
  • Journal Title: FEMS Microbiol. Lett. 298 Pages: 118-123
  • Peer Reviewed
• [Journal Article] Comparable studies of immunostimulating activities in vitro among Mycobacterium bovis bacillus Calmette-Guerin (BCG) sub-strains (2009)
  • Author(s): D. Hayashi
  • Journal Title: FEMS Immunol. Med. Microbiol. (In press)
  • Peer Reviewed
• [Journal Article] Mycobacterium kyorinense sp. nov., a novel slowly growing Mycobacterium sp. related to Mycobacterium celatum isolated from human clinical specimens. (2009)
  • Author(s): M. Okazaki
  • Journal Title: Int. J. Syst. Evol. Microbiol. (In press)
  • Peer Reviewed
• [Journal Article] Identification and characterization of two novel methyltransferase genes that determine the serotype 12-specific structure of glycopeptidolipids of Mycobacterium intracellulare. (2008)
  • Author(s): N. Nakata, N. Fujiwara, T. Naka, I. Yano, K. Kobayashi, S. Maeda
  • Journal Title: J. Bacteriol 190 Pages: 1064-1071
  • Peer Reviewed
• [Journal Article] Structural analysis and biosynthesis gene cluster of an antigenic glycopeptidolipid from Mycobacterium intracellulare. (2008)
  • Author(s): N. Fujiwara, N. Nakata, T. Naka, I. Yano, M. Doe, D. Chatterjee, M. McNeil, P.J. Brennan, K. Kobayashi, M. Makino, S. Matsumoto, H. Ogura, S. Maeda
  • Journal Title: J. Bacteriol 190 Pages: 3613-3621
  • Peer Reviewed
• [Journal Article] Structural analysis and biosynthesis gene cluster of an antigenic glycopeptidolipid from Mycobacterium intracellulare (2008)
  • Author(s): N. Fujiwara
  • Journal Title: Journal of Bacteriology 190 Pages: 1063-1071
  • Peer Reviewed
• [Journal Article] Identification and characterization of two novel methyltransferase genes that determine the serotype 12-specific structure of glycopeptidolipids of Mycobacterium intracellulare. (2008)
  • Author(s): N. Nakata
  • Journal Title: Journal of Bacteriology 190 Pages: 1064-1071
  • Peer Reviewed
• [Journal Article] Control of cell wall assembly by a histone-like protein in mycobacteria. (2007)
  • Author(s): T. Katsube, S. Matsumoto, M. Takatsuka, M. Okuyama, Y. Ozeki, M. Naito, Y. Nishiuchi, N. Fujiwara, M. Yoshimura, T. Tsuboi, M. Torii, N. Oshitani, T. Arakawa, K. Kobayashi
  • Journal Title: J. Bacteriol 189 Pages: 8241-8249
  • Peer Reviewed
• [Journal Article] Control of cell wall assembly by a histone-like protein in mycobacteria. (2007)
  • Author(s): T. Katsube
  • Journal Title: Journal of Bacteriology 189 Pages: 8241-8249
  • Peer Reviewed
• [Presentation] kasB結核菌の透過電子顕微鏡観察 (2010)
  • Author(s): 山田博之
  • Organizer: 第34回日本顕微鏡学会関東支部会
  • Place of Presentation: 帝京大学医学部板橋キャンパス(東京)
  • Year and Date: 2010-03-20
• [Presentation] Mycobacterium smegmatis株間の脂質分布と脂質成分による宿主応答の差異 (2010)
  • Author(s): 藤原永年, 野本摩利, 中崇, 合田麗奈, 小川みどり, 水野淨子, 小林貴美子, 谷口初美
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] Mycobacterium avium complex血清型4型株におけるglycopeptidolipidの生合成解析 (2010)
  • Author(s): 宮本友司, 向井徹, 甲斐雅規, 前田百美, 中崇, 藤原永年, 水野淨子, 矢野郁也, 牧野正彦
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] Mycobacterium smegmatis株間の脂質の分布と脂質成分による宿主応答の差異 (2010)
  • Author(s): 藤原永年
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Mycobacterium avium complex血清型4型株におけるglycopeptidolipidの生合成解析 (2010)
  • Author(s): 宮本友司
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Structures and host recognitions of antigenic serotype 7, 13 glycopeptidolipids from Mycobacterium intracellulare. (2009)
  • Author(s): N. Fujiwara, T. Naka, R. Goda, S. Mizuno, M. Yoshimura, S. Matsumoto, H. Ogura, S. Maeda
  • Organizer: Infection 2009
  • Place of Presentation: Birmingham, UK
• [Presentation] MAC血清型13型菌由来糖ペプチド脂質の構造修飾と宿主応答 (2009)
  • Author(s): 合田麗奈, 中崇, 前田伸司, 水野淨子, 小林貴美子, 藤原永年
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Rhodococcus equi - immunity and strategies for prevention. (2009)
  • Author(s): S.A. Hines, S.P. Harris. N. Fujiwara, R.H. Mealey, R.G. Dossa, D.C. Alperin
  • Organizer: The 5th International Veterinary Vaccines and Diagnostics Conference (IVVDC)
  • Place of Presentation: Madison, WI USA
• [Presentation] Mycobacterium avium TMC724SのpH依存的な集落形成の違いと免疫応答、薬剤感受性、細胞壁形成との相関 (2009)
  • Author(s): 山本龍二, 堀田康弘, 菅原勇, 藤原永年, 伊藤佐生智, 瀧井猛将
  • Organizer: 第84回日本結核病学会総会
  • Place of Presentation: 札幌
• [Presentation] Mycobacterium intracellulare由来血清型7, 12, 13型糖ペプチド脂質の構造類似性とオリゴ糖解析 (2009)
  • Author(s): 藤原永年, 中田登, 中崇, 水野淨子, 牧野正彦, 松本壮吉, 前田伸司
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Mycobacterium avium TMS724SのpH依存的形態変化に関する研究 (2009)
  • Author(s): 堀田康弘, 瀧井猛将, 菅原勇, 藤原永年, 山本龍二, 稲垣衣美, 小野嵜菊夫
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Mycobacterium bovis BCG亜株間の自然免疫誘導活性の差異とミコール酸の関与 (2009)
  • Author(s): 林大介, 安田恵実, 瀧井猛将, 藤原永年, 山本三郎, 矢野郁也, 小野嵜菊夫
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] BCG菌のミコール酸サブクラス合成遺伝子の解析 (2009)
  • Author(s): 甲斐雅規, 藤原永年, 宮本友司, 向井徹, 矢野郁也, 牧野正彦
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Rhodococcus equi-immunity and strategies for prevention (2009)
  • Author(s): Hines, S.A.
  • Organizer: The 5th International Veterinary Vaccines and Diagnostics Conference (IVVDC)
  • Place of Presentation: Madison, WI, USA
• [Presentation] Structures and host recognitions of antigenic serotype 7, 13 glycopeptidolipids from Mycobacterium intracellulare (2009)
  • Author(s): Fujiwara, N.
  • Organizer: Infection 2009
  • Place of Presentation: Birmingham, UK.
• [Presentation] Mycobacterium avium TMC724SのpH依存的な集落形成の違いと免疫応答、薬剤感受性、細胞壁形成との相関 (2009)
  • Author(s): 山本龍二
  • Organizer: 第84回日本結核病学会総会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
• [Presentation] MAC血清型13型菌由来糖ペプチド脂質の構造修飾と宿主応答 (2009)
  • Author(s): 合田麗奈
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
• [Presentation] Mycobacterium intracellulare serotype13 由来新規特異糖ペプチド脂質の糖鎖構造と生合成 (2008)
  • Author(s): 藤原永年
  • Organizer: 第81回日本生化学会大会 第31回日本分子生物学会年会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Three methyltransferase genes determine the divergence between Mycobacterium intracellulare serotypes 7 and 12 (2008)
  • Author(s): N. Nakata
  • Organizer: 108^<th> General Meeting, The American Society for Microbiology
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-06-03
• [Presentation] Genetic analysis of the glycosylation pathway of glycopeptidolipids in Mycobacterium intracellulare serotype 16 and serotype 17 (2008)
  • Author(s): S. Maeda
  • Organizer: 108^<th> General Meeting, The American Society for Microbiology
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-06-03
• [Presentation] Structure and biosynthesis gene cluster of an antigenic serotype 16 glycopeptidolipid from Mycobacterium intracellulare (2008)
  • Author(s): N. Fujiwara
  • Organizer: 108^<th> General Meeting, The American Society for Microbiology
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-06-02
• [Presentation] Mycobacterial DNA-binding protein 1(MDP1)による増殖と細胞壁合成の同調メカニズム (2008)
  • Author(s): 松本壮吉
  • Organizer: 第83回日本結核病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-04-25
• [Presentation] Mycobacterium bovis BCG亜株間の生化学的、物理化学的性質と生物学的活性の比較研究 (2008)
  • Author(s): 瀧井猛将
  • Organizer: 第83回日本結核病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-04-24
• [Presentation] Mycobacterium intracellulare由来血清型7,12型glycopeptidolipid糖鎖合成遺伝子の機能解析 (2008)
  • Author(s): 藤原 永年
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-24
• [Presentation] Mycobacterium intracellular血清型12のglycopeptidolipid生合成遺伝子領域の解析 (2008)
  • Author(s): 中田 登
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-24
• [Presentation] Mycobacterium intracellulare serotype 13由来新規特異糖ペプチド脂質の糖鎖構造と生合成 (2008)
  • Author(s): 水野淨子, 中崇, 中田登, 前田伸司, 合田麗奈, 小林貴美子, 牧野正彦, 藤原永年
  • Organizer: 第81回日本生化学会大会第31回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸
• [Presentation] Structure and biosynthesis gene cluster of an antigenic serotype 16 glycopeptidolipid from Mycobacterium intracellulare. 108th General Meeting (2008)
  • Author(s): N. Fujiwara, N. Nakata, T. Naka, I. Yano, K. Kobayashi, M. Makino, S. Matsumoto, M. Yoshimura, S. Maeda
  • Organizer: The American Society for Microbiology
  • Place of Presentation: Boston USA
• [Presentation] Three methyltransferase genes determine the divergence between Mycobacterium intracellulare serotypes 7 and 12. (2008)
  • Author(s): N. Nakata, N. Fujiwara, S. Maeda, T. Naka, I. Yano, M. Makino
  • Organizer: 108th General Meeting, The American Society for Microbiology
  • Place of Presentation: Boston USA
• [Presentation] Genetic analysis of the glycosylation pathway of glycopeptidolipids in Mycobacterium intracellulare serotype 16 and serotype 17. (2008)
  • Author(s): S. Maeda, N. Nakata, T. Naka, I. Yano, M. Makino, N. Fujiwara
  • Organizer: 108th General Meeting, The American Society for Microbiology
  • Place of Presentation: Boston USA
• [Presentation] BCG亜株の脂質生化学的比較研究 (2008)
  • Author(s): 藤原永年, 松本壮吉, 瀧井猛将, 藤田由希子, 矢野郁也, 前田伸司, 山本三郎
  • Organizer: 第83回日本結核病学会総会
  • Place of Presentation: 東京
• [Presentation] Mybacterium bovis BCG亜株間の生化学的、物理化学的性質と生物学的活性の比較研究 (2008)
  • Author(s): 瀧井猛将, 藤原永年, 矢野郁也, 山本三郎
  • Organizer: 第83回日本結核病学会総会
  • Place of Presentation: 東京
• [Presentation] Mycobacterium intracellulare由来血清型7, 12型glycopeptidolipid糖鎖合成遺伝子の機能解析 (2008)
  • Author(s): 藤原永年, 中田登, 前田伸司, 中崇, 水野淨子, 牧野正彦, 松本壮吉, 矢野郁也
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
• [Presentation] Mycobacterium intracellulare血清型12のglycopeptidolipid生合成遺伝子領域の解析 (2008)
  • Author(s): 中田登, 藤原永年, 前田伸司, 中崇, 矢野郁也, 小林和夫, 牧野正彦
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
• [Presentation] Structural analysis of sphingoglycolipid from Basidiomycota (2007)
  • Author(s): S. Mizuno, T. Naka, K. Kobayashi, M. Doe, S. Maeda, N. Fujiwara
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] ミコール酸分子種の異なるcord factorの宿主応答 (2007)
  • Author(s): 藤原永年, 前田伸司, 矢野郁也, 松本壮吉, 小林和夫
  • Organizer: 第82回日本結核病学会総会
  • Place of Presentation: 大阪
• [Book] Mycobacterial glycolipids and host responses(E. Sasaki (ed.)) (2008)
  • Author(s): N. Fujiwara, K. Kobayashi
  • Publisher: Nova Science Publishers
• [Book] KEY WORD感染症[第2版](山口恵三・戸塚恭一(編)) (2008)
  • Author(s): 前田伸司, 藤原永年
  • Publisher: 先端医学社
• [Book] Mycobacterial glycolipids and host responses, In E. Sasaki (ed. ), Glycolipids : New Research.(Chapter IV) (2008)
  • Author(s): N. Fujiwara
  • Publisher: Nova Science Publishers, Inc., Hauppauge NY.