Genetic bases for the emergence of community-associated MRSA strains

• Project/Area Number: 19590456
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Juntendo University
• Principal Investigator: ITO Teruyo Juntendo University, 医学部, 准教授 (10095763)
• Research Collaborator: 韓 笑 順天堂大学, 医学研究科, 感染制御科学大学院生
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: MRSA / 市中感染症 / コンピテンス / 耐性伝達

Research Abstract

近年世界中に蔓延しつつある市中感染型MRSAの出現をそのSCCmecの構造解析と遺伝子伝達実験の2つの方向から解析をした。その結果、市中感染型MRSAのもつ多様なSCCmecの構造を明らかにし、SCCmecの形成を考察する貴重は知見を得た。即ち、ブドウ球菌属のもつSCCと呼ぶ動く遺伝因子に、mec遺伝子複合体がcomposite transposonとして転移したという説を裏付ける新しい傍証を提供した。しかし実験的にその形成過程を解明する目的は、現段階は、終了したとは言えずその途上にある。本研究で明らかにし得た遺伝子伝播に関する知見は以下に要約される。
(1) Phage transductionによるSCCmecの伝達はおこるが、その頻度は低い。本実験で検討した限り、SCCmecの伝達は、全ての全領域が伝達された場合のみ成功している。
(2) Phageの吸着により、competencyに関与する遺伝子の発現が上昇する。
(3) conjugative plasmidの伝達に伴ってトランスポゾンTn554の転移が促進され、ともに伝達される。しかし、メチシリン耐性に関与する遺伝子はこの限りではない。

Research Products

• [Journal Article] Recurrence of heterogeneous methicillin-resistant Staphylococcus aureus (MRSA) among the MRSA clinical isolates in a Japanese university hospital (2009)
  • Author(s): Kishii K, Ito T, Watanabe S, Okuzumi K, Hiramatsu K
  • Journal Title: J Antimicrob Chemother 62(2) Pages: 324-328
• [Journal Article] Identification of a novel variant of SCCmec, type-II. 5, and its truncated form by the insertion of putative conjugative transposon Tn6012 (2009)
  • Author(s): Han X, Ito T, Takeuchi F, Ma XX, Takasu M, Uehara Y, Oliveira DC, de Lencastre H, Hiramatsu K
  • Journal Title: Antimicrob Agents Chemother [Epubahead of print]
• [Journal Article] Novel type of staphylococcal cassette chromosome mec in a methicillin-resistant Staphylococcus aureus strain isolated in Sweden (2008)
  • Author(s): Berglund C, Ito T, Ikeda M, Ma XX, Soderquist B, Hiramatsu K
  • Journal Title: Antimicrob Agents Chemother 52 Pages: 3512-6
• [Journal Article] A Latex Agglutination Assay for Specific Detection of Panton-Valentine Leukocidin (2008)
  • Author(s): Kanenari Oishi, Tadashi Baba, Yasuo Nakatomi, Teruyo Ito, and Keiichi Hiramatsu
  • Journal Title: J Microbiol Methods 75(3) Pages: 411-5
• [Journal Article] Two different PVL phage lineages predominate in Japan (2008)
  • Author(s): Xiao Xue Ma, Teruyo Ito, et al.
  • Journal Title: J Clin Microbiol 46(10) Pages: 3246-58
• [Journal Article] A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus (2008)
  • Author(s): Kaito C, Omae Y, Matsumoto Y, Nagata M, Yamaguchi H, Aoto T, Ito T, Hiramatsu K, Sekimizu K
  • Journal Title: PLoS ONE 3(12) Pages: 3921-3921
• [Journal Article] Extreme genetic diversity of methicillin-resistant Staphylococcus epidermidis (MRSE) disseminated amonghealthy Japanese children (2008)
  • Author(s): Jamaluddin TZ, Kuwahara-Arai K, Hisata K, Terasawa M, Cui L, Baba T, Sotozono C, Kinoshita S, Ito T, Hiramatsu K
  • Journal Title: J Clin Microbiol 46(11) Pages: 3778-83
• [Journal Article] Novel type of staphylococcal cassette chromosome mec in amethicillin-resistant Staphylococcus aureus strain isolated in Sweden. (2008)
  • Author(s): Berglund C, Ito T, Ikeda M, Ma XX, Soderquist B, Hiramatsu K.
  • Journal Title: Antitnicrob Agents Chemother 52 Pages: 3512-6
  • Peer Reviewed
• [Journal Article] Recurrence of heterogeneous methicillin-resistant Staphylococcus aureus (MRSA) among the MRSA clinical isolates in a Japanese university hospital. (2008)
  • Author(s): Kishii K, Ito T, Watanabe S, Okuzumi K, Hiramatsu K.
  • Journal Title: J Antimicrob Chemother 62(2) Pages: 324-328
  • Peer Reviewed
• [Journal Article] Multiplex PCRs for Assignment of Staphylocoagulase Types and Subtypes of Type VI Staphylocoagulase (2008)
  • Author(s): Fumihiko Sakai, (8 members) Teruyo Ito
  • Journal Title: J Microbiol Methods. 75(2) Pages: 312-317
  • Peer Reviewed
• [Journal Article] A Latex Agglutination Assay for Specific Detection of Panton-Valentine Leukocidin (2008)
  • Author(s): Kanenari Oishi, Tadashi Baba, Yasuo Nakatomi, Teruyo Ito, Keiichi Hiramatsu
  • Journal Title: J Microbiol Methods. 75(3) Pages: 411-5
  • Peer Reviewed
• [Journal Article] A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. (2008)
  • Author(s): Kaito C, Omae Y, Matsumoto Y, Nagata M, Yamaguchi H, Aoto T, Ito T, Hiramatsu K, Sekimizu K.
  • Journal Title: PLoS ONE. 3(12)
  • Peer Reviewed
• [Journal Article] Extreme genetic diversity of methicillin-resistant Staphylococcus epidermidis (MRSE) disseminated among healthy Japanese children. (2008)
  • Author(s): Jamaluddin TZ, Kuwahara-Arai K, Hisata K, Terasawa M, Cui L, Baba T, Sotozono C, Kinoshita S, Ito T, Hiramatsu K.
  • Journal Title: J Clin Microbiol 46(11) Pages: 3778-83
  • Peer Reviewed
• [Journal Article] Susceptibilities of healthcare- and community-associated methicillin-resistant staphylococci to the novel des-F(6)-quinolone DX-619 (2007)
  • Author(s): Watanabe S, Ito T, Hiramatsu K
  • Journal Title: J Antimicrob Chemother 60 Pages: 1384-1387
• [Journal Article] Precise excision and self-integration of a composite transposon as a model for spontaneous large-scale chromosome inversion/deletion of the Staphylococcus haemolyticus clinical strain JCSC1435 (2007)
  • Author(s): Watanabe S, Ito T, Morimoto Y, Takeuchi F, Hiramatsu K
  • Journal Title: J Bacteriol 189 Pages: 2921-2925
• [Journal Article] Combination of multiplex PCRs for SCCmec type assignment : Rapid identification system for mec, ccr, and major differences in junkyard regions (2007)
  • Author(s): Yoko Kondo, Teruyo Ito, (4 members), and Keiichi Hiramatsu
  • Journal Title: Antimicrob Agents Chemother 51 Pages: 264-274
• [Journal Article] Development and validation of microarray-based assay for epidemiological study of MRSA (2007)
  • Author(s): Otsuka J, (3 members), Ito T, (4 members), Tashiro H
  • Journal Title: Mol Cell Probes Pages: 1-13
• [Journal Article] Rapid detection of Staphylococcus aureus strains having reducedsu sceptibility to vancomycin using a chemiluminescence-based drug-susceptibility test (2007)
  • Author(s): Tajima Y, Komatsu M, Ito T, Hiramatsu K
  • Journal Title: J Microbiol Methods 70 Pages: 434-441
• [Journal Article] Susceptibilities ofhealthcare-and community-associated methicillin-resistantstaphylococci to the novel des-F(6)-quinolone DX-619 (2007)
  • Author(s): Watanabe S, Ito T, Hiramatsu K.
  • Journal Title: J Antimicrob Chemother 60 Pages: 1384-1387
  • Peer Reviewed
• [Journal Article] Precise excision and self-integration of a composite transposon as a model for spontaneous large-scale chromosome inversion deletion ofthe Staphylococcus haemolyticus clinical strain JCSC 1435 (2007)
  • Author(s): Watanabe S, Ito T, Morimoto Y, Takeuchi F, Hiramatsu K.
  • Journal Title: J Bacteriol 189 Pages: 2921-2925
  • Peer Reviewed
• [Journal Article] Combination of multiplex PCRs for SCCmec type assignment:Rapid identification system for mec,ccr,and major differences in junkyard regions (2007)
  • Author(s): Yoko Kondo, Teruyo Ito^<1*>, (4members), Keiichi Hiramatsu
  • Journal Title: Antimicrob Agents Chemother 51 Pages: 264-274
  • Peer Reviewed
• [Journal Article] Development and validation ofmicroarray-based assay for epidemiological study of MRSA (2007)
  • Author(s): Otsuka J, (3 members), Ito T, (4members), Tashiro H.
  • Journal Title: Mol Cell Probes 22 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Rapid detection of Staphylococcus aureus strains having reduced susceptibility to vancomycin using a chemiluminescence-based drug-susceptibility test (2007)
  • Author(s): Tajima Y, Komatsu M, Ito T, Hiramatsu K.
  • Journal Title: J Microbiol Methods 70 Pages: 434-441
  • Peer Reviewed
• [Journal Article] Multiplex PCRs for Assignment of Staphylocoagulase Types and Subtypes of Type VI Staphylocoagulase
  • Author(s): Fumihiko Sakai, (8 members) Teruyo Ito
  • Journal Title: J Microbiol Methods 75(2) Pages: 312-317
• [Presentation] S. haemolyticusにおけるcassette chromosomeの集積と進化 (2008)
  • Author(s): 韓笑、伊藤輝代、他
  • Organizer: 第53回ブドウ球菌研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-19
• [Presentation] コアグラーゼ遺伝子にもとづくタイピング (2008)
  • Author(s): Teruyo Ito
  • Organizer: 第53回ブドウ球菌研究会
  • Place of Presentation: 東京大学
  • Year and Date: 2008-09-17
• [Presentation] What have we learnt from the structural comparison of SCC elements (2008)
  • Author(s): Teruyo Ito
  • Organizer: 13th ISSSI
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2008-09-07
• [Presentation] What have we learnt from the structural comparison of SCC elements? (2008)
  • Author(s): Teruyo Ito
  • Organizer: 13^<th> ISSSI
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2008-09-07
• [Presentation] 高知大学病院で分離されたMRSAの持つ新規SCCmec (2008)
  • Author(s): 韓笑、伊藤輝代、他
  • Organizer: 第81回日本細菌学会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-25
• [Presentation] スエーデンで分離された市中獲得MRSAの保持するSCCmec (2008)
  • Author(s): 伊藤輝代、馬笑雪、渡辺真弥、平松啓一
  • Organizer: 第81回日本細菌学会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-24
• [Presentation] Characterization of Methicillin-Resistant (2007)
  • Author(s): Kondo Y, Ito T, Ma XX, Watanabe S, and Hiramatsu K
  • Organizer: Staphylococcus aureus strains isolated in a Japanese geriatric hospital in early 1980s APIC
  • Place of Presentation: USA
• [Presentation] Staphylococcus haemolyticusのマンノース及びN-アセチルグルコサミンによる増殖抑制 (2007)
  • Author(s): 渡邊真弥, 伊藤輝代, 平松啓一
  • Organizer: 第52回日本ブドウ球菌研究会
  • Place of Presentation: 徳島大学長井記念ホール
• [Presentation] 挿入配列が引き起こすStaphylococcus haemolyticusゲノムの大規模ゲノムリアレンジメント (2007)
  • Author(s): 渡邊真弥, 伊藤輝代, 森本ゆふ, 竹内史比古, 平松啓一
  • Organizer: 第19回微生物シンポジウム
  • Place of Presentation: 東京大学本郷キャンパス薬学系研究科総合研究棟講堂
• [Presentation] 挿入配列の転移酵素により引き起こされたStaphylococcus haemolyticusゲノムの逆位と欠失 (2007)
  • Author(s): 渡邊真弥, 伊藤輝代, 森本ゆふ, 竹内史比古, 平松啓一
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: アジア太平洋トレードセンター
• [Presentation] ブドウ球菌属に存在するSCCmecの構造解析 (2007)
  • Author(s): 伊藤輝代、馬笑雪、韓笑、近藤陽子、星最智、岸井こずえ、桑原京子、平松啓一
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: アジア太平洋トレードセンター
• [Presentation] 二つのPVLファージの我が国における分布 (2007)
  • Author(s): 馬笑雪、伊藤輝代、平松啓一
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: アジア太平洋トレードセンター
• [Presentation] CEZ感受性MRSAの薬剤感受性傾向及び遺伝学的性状について (2007)
  • Author(s): 岸井こずえ、伊藤輝代、奥住律子、平松啓一
  • Organizer: 第80回日本細菌学会総会
  • Place of Presentation: アジア太平洋トレードセンター
• [Presentation] Characterization of Methicillin-Resistant Staphylococcus aureus strainsisolated in a Japanese geriatric hospital in early 1980s (2007)
  • Author(s): Kondo Y, Ito T, Ma XX, Watanabe S, Hiramatsu K
  • Organizer: APIC
  • Place of Presentation: USA
• [Presentation] Analysis of SCCmec elements carried by MRSA strains isolated from veterinarians
  • Author(s): Teruyo Ito, Shanshuang Li, Anders Rhod Larsen, Robert Leo Skov, Han Xio, Keiichi Hiramatsu
  • Organizer: 48th ICAAC Washington
  • Place of Presentation: USA
• [Book] Methicillin-Resistat Staphylococcus aureus (MRSA) Protocols ; Methods in Molecular Biology (2007)
  • Author(s): Ito, I., K. Kuwahara, and K. Hiramatsu
  • Total Pages: 391
  • Publisher: Humana press
• [Book] Methicillin-Resistat Staphylococcus aureus(MRSA)Protocols;Methods in Molecular Biology 391 (2007)
  • Author(s): Ito, L, K. Kuwahara, K. Hiramatsu
  • Total Pages: 264
  • Publisher: Humana press
• [Remarks]
  • URL: http://www.staphylococcus.net
• [Remarks]
  • URL: http://www.staphylococcus.net/