The investigation on expression and regulation of pathogenic invasive gene in dermatophytes using new transformation system.

• Project/Area Number: 19590457
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Teikyo University
• Principal Investigator: MAKIMURA Koichi Teikyo University, 医学部, 准教授 (00266347)
• Co-Investigator(Kenkyū-buntansha): YAMADA Tsuyoshi 帝京大学, 医真菌研究センター, 講師 (80424331) ; NISHIYAMA Yayoi 帝京大学, 医療技術学部, 教授 (10082231)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 病原性 / 白癬菌 / 動物モデル / 形質転換 / 病原因子 / 遺伝子破壊 / 遺伝子診断 / 白癬 / プロテアーゼ / 選択マーカ / アグロバクテリウム法

Research Abstract

Recently the number of patients affected by dermatophytosis are estimated more than 20% in Japan. For investigation on invasive (pathogenic) factor of dermatopytes, development of methods for destruction of target gene of the fungus is essential. In this study we constructed the original methods which are useful for investigation of invasive factor gene in major dermatophytes ; Trichophyton mentagrophytes using animal infectious models. At first we reported the new gene transformation system (Agrobacterium method) using Agrobacterium tumefaciens. Next we construct G418 as the marker of the multiple genes transformation. Finally we developed the Tmku80-destroyed Trichophyton, #49 strain. This strain has destroyed gene Tmku80 which is homolog of ku80 gene of T. mentagrophytes TIMM2789. By making #49 strain, specific gene destruction became possible, and also we could select target gene freely. At the same time we could compare the pathogenesis between modified gene strain and wild type strain of Trichophyton using experimental animal infectious models. Continuously we developed and reported a gene diagnosis system which is useful for human dermatophytosis. The result of this study would contribute to the analysis of clinical condition of dermatophytosis and progress of protection, diagnosis and therapy of the infection.

Research Products

• [Journal Article] Rapid identification of Prototheca zopfii by nested polymerase chain reaction based on the nuclear small subunit ribosomal DNA. (2009)
  • Author(s): Onozaki M, Makimura K, Hasagawa A
  • Journal Title: Journal of Dermatological Science 54(1) Pages: 56-59
  • NAID: 10025333247
• [Journal Article] In vitro antifungal activities of luliconazole, a new topical imidazole (2009)
  • Author(s): Koga H, Nanjoh Y, Makimura K, Tsuboi R
  • Journal Title: Medical Mycology 47(6) Pages: 640-647
• [Journal Article] Agrobacterium tumefaciens-mediated transformation of the dermatophyte, Trichophyton mentagrophytes: an efficient tool for gene transfer. (2009)
  • Author(s): Yamada T, Makimura K, Satoh K, Umeda Y, Ishihara Y, Abe S
  • Journal Title: Medical Mycology 47(5 Pages: 485-494
• [Journal Article] Evaluation of a new rapid molecular diagnostic system for Plasmodium falciparum combined with DNA filter paper, loop-mediated isothermal amplification (LAMP) and melting curve analysis (2009)
  • Author(s): Yamamura M, Makimura K, Ota Y
  • Journal Title: Japanese Journal of Infectious Diseases 62(1) Pages: 20-25
• [Journal Article] Direct Colony PCR of Several Medically Important Fungi using AmpdirectR Plus. (2009)
  • Author(s): AlShahni MM, Makimura K, Yamada T, Satoh K, Ishihara Y, Takatori K, Sawada T
  • Journal Title: Japanese Journal of Infectious Diseases 62(2) Pages: 164-167
• [Journal Article] Comparative study of direct PCR, microscopic examination, and culture-based morphological methods for detection and identification of dermatophytes in nail and skin samples (2009)
  • Author(s): Uchida T, Makimura K, Ishihara K, Goto H, Tajiri Y, Okuma M, Fujisaki R, Uchida K, Abe S, Iijima M
  • Journal Title: ournal of Dermatology 36(4) Pages: 202-208
• [Journal Article] Is Bjerkandera adusta important to fungus-associated chronic cough as an allergen? (2009)
  • Author(s): Ogawa H, Fujimura M, Takeuchi Y, Makimura K
  • Journal Title: Eight cases report. Journal of Asthma 46(8) Pages: 849-55
• [Journal Article] Efficacy of itraconazole in the treatment of patients with chronic cough whose sputa yield basidiomycetous fungi (2009)
  • Author(s): Ogawa H, Fujimura M, Takeuchi Y, Makimura K
  • Journal Title: fungus-associated chronic cough (FACC) Journal of Asthma 46(4) Pages: 407-12
• [Journal Article] Serum Galactomannan Negative Disseminated Aspergillosis In An Intensive Care Unit (2009)
  • Author(s): Saito T, Shime N, ItohK, Fujita N, Saito Y, Shinozaki M, Shibuya K, Makimura K, Hashimoto S
  • Journal Title: A Case Report. Infection (in press)
• [Journal Article] Molecular detection of dermatophytes and nondermatophytes in onychomycosis by nested PCR based on 28S ribosomal RNA gene sequences. (2009)
  • Author(s): Ebihara M, Makimura K, Sato K, Abe S,, Tsuboi R
  • Journal Title: British Journal of Dermatology 161(5) Pages: 1038-1044
• [Journal Article] Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay (2009)
  • Author(s): Sugita T, Suzuki M, Goto S, Nishikawa A, Hiruma M, Yamazaki T, Makimura K
  • Journal Title: Medical Mycology May 21 Pages: 1-5
• [Journal Article] The importance of basidiomycetous fungi cultured from sputum of chronic idiopathic cough (2009)
  • Author(s): Ogawa H, Fujimura M, Takeuchi Y, Makimura K
  • Journal Title: Respiratory Medicine 103(10) Pages: 492-7
• [Journal Article] Polymerase chain reaction assay for specific identification of Candida guilliermondii (Pichia guilliermondii) (2009)
  • Author(s): Yamamura M, Makimura K, Fujisaki R, Satoh K, Kawakami S, Nishiya H, Ota Y.
  • Journal Title: J Infect Chemother. 15(4) Pages: 214-8
  • NAID: 10025129192
• [Journal Article] Prototheca cutis sp. nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin. (2009)
  • Author(s): Satoh K, Ooe K, Nagayama H, Makimura K.
  • Journal Title: Int J Syst Evol Microbiol. (in press)
• [Journal Article] Enhanced gene replacements in Ku80 disruption mutants of the dermatophyte, Trichophyton mentagrophytes. (2009)
  • Author(s): Yamada T, Makimura K, Hisajima T, Ishihara Y, Umeda Y, Abe S.
  • Journal Title: FEMS Microbiol Lett. 298(2) Pages: 208-17
• [Journal Article] Isolation and identification of Rhizomucor pusillus from pleural zygomycosis in an immunocompetent patient. (2009)
  • Author(s): Kimura M, Udagawa SI, Makimura K, Satoh K, Toyazaki N, Ito H.
  • Journal Title: Medical Mycology 47(8) Pages: 869-873
• [Journal Article] 26S rDNA-based phylogenetic investigation of Japanese cattle-associated Prototheca zopfii isolates. (2009)
  • Author(s): Kishimoto Y, Kano R, Maruyama H, Onozaki M, Makimura K, Ito T, Matsubara K, Hasegawa A, Kamata H.
  • Journal Title: The Journal of Veterinary Medical Science (in press)
  • NAID: 130000134515
• [Journal Article] Nourseothricin acetyltransferase: a new dominant selectable marker for the dermatophyte Trichophyton mentagrophytes. (2009)
  • Author(s): Alshahni M, Makimura K, Yamada T, Takatori K, Sawada T.
  • Journal Title: Medical Mycology (in press)
• [Journal Article] Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital. (2009)
  • Author(s): Satoh K, Makimura K, Hasumi Y, Nishiyama Y, Uchida K, Yamaguchi H.
  • Journal Title: Microbiology and Immunology 53(1) Pages: 41-4
  • NAID: 10026404038
• [Journal Article] Human External Ear Canal as the Specific Reservoir of Malassezia slooffiae (2009)
  • Author(s): Kaneko T, Shiota R, Shibuya S, Watanabe S, Umeda Y, Takeshita K, Yamamoto M, Nishioka K, Makimura K
  • Journal Title: Medical Mycology (in press)
• [Journal Article] Typing of Aspergillus fumigatus and Aspergillus niger Strains by Random Amplification of Polymorphic DNA Analysis Using a Six Primer Set. (2009)
  • Author(s): Mirhendi H, Moazeni M, Nikaeen M, Makimura K.
  • Journal Title: Shiraz E-Medical Journal 10(4) Pages: 190-200
• [Journal Article] Rapid identification of Prototheca zopfii by nested polymerase chain reaction based on the nuclear small subunit ribosomal DNA. (2009)
  • Author(s): Onozaki M
  • Journal Title: Journal of Dermatological Science 54(1) Pages: 56-59
  • Peer Reviewed
• [Journal Article] In vitro antifungal activities of luliconazole, a new topical imidazole. (2009)
  • Author(s): Koga H
  • Journal Title: Medical Mycology 47(6) Pages: 640-647
  • Peer Reviewed
• [Journal Article] Agrobacterium tumefaciens-mediated transformation of the dermato phyte, Trichophyton mentagrophytes : an efficient tool for gene transfer. (2009)
  • Author(s): Yamada T
  • Journal Title: Medical Mycology 47(5) Pages: 485-494
  • Peer Reviewed
• [Journal Article] Evaluation of a new rapid molecular diagnostic system for Plasmodium falciparum combined with DNA filter paper, loop-mediated is othermal amplification (LAMP) and melting curve analysis. (2009)
  • Author(s): Yamamura M
  • Journal Title: Japanese Journal of Infectious Diseases 62(1) Pages: 20-25
  • Peer Reviewed
• [Journal Article] Direct Colony PCR of Several Medically Important Fungi using A mpdirect(R) Plus. (2009)
  • Author(s): AlShahni MM
  • Journal Title: Japanese Journal of Infectious Diseases 62(2) Pages: 164-167
  • Peer Reviewed
• [Journal Article] Comparative study of direct PCR, microscopic examination, and culture-based morphological methods for detection and identification of dermatophytes in nail and skin samples. (2009)
  • Author(s): Uchida T
  • Journal Title: Journal of Dermatology 09236(4) Pages: 202-208
  • Peer Reviewed
• [Journal Article] Is Bjerkandera adusta important to fungus-associated chronic cough as an allergen? Eight cases report. (2009)
  • Author(s): Ogawa H
  • Journal Title: Journal of Asthma 46(8) Pages: 849-855
  • Peer Reviewed
• [Journal Article] Efficacy of itraconazole in the treatment of patients with chroniccough whose sputa yield basidiomycetous fungi; fungus-associated chronic cough (FACC) (2009)
  • Author(s): Ogawa H
  • Journal Title: Journal of Asthma 46(4) Pages: 407-412
  • Peer Reviewed
• [Journal Article] A Suggested Pathogenic Role of Trichosporon montevideense in a Case of Onychomycosis in a Japanese Monkey. (2009)
  • Author(s): AlShahni MM
  • Journal Title: Journal of Veterinary Medical Science 71(7) Pages: 983-986
  • Peer Reviewed
• [Journal Article] Molecular detection of dermatophytes and nondermatophytes in onychomycosis by nested PCR based on 28S ribosomal RNA gene sequences. (2009)
  • Author(s): Ebihara M
  • Journal Title: British Journal of Dermatology 161(5) Pages: 1038-1044
  • Peer Reviewed
• [Journal Article] Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay. (2009)
  • Author(s): Sugita T
  • Journal Title: Medical Mycology 21 Pages: 1-5
  • Peer Reviewed
• [Journal Article] The importance of basidiomycetous fungi cultured from sputum of chronic idiopathic cough. (2009)
  • Author(s): Ogawa H
  • Journal Title: Respiratory Medicine 103(10) Pages: 1492-1497
  • Peer Reviewed
• [Journal Article] Polymerase chain reaction assay for specific identification of Candida guilliermondii (Pichia guilliermondii). (2009)
  • Author(s): Yamamura M
  • Journal Title: Infect Chemother. 15(4) Pages: 214-218
  • Peer Reviewed
• [Journal Article] Enhanced gene replacements in Ku80 disruption mutants of the dermatophyt e, Trichophyton mentagrophytes. (2009)
  • Author(s): Yamada T
  • Journal Title: FEMS Microbiol Lett. 298(2) Pages: 208-217
  • Peer Reviewed
• [Journal Article] Isolation and identification of Rhizomucor pusillus from pleural zygomycosis in an immunocompetent patient. (2009)
  • Author(s): Kimura M
  • Journal Title: Medical Mycology 47(8) Pages: 869-873
  • Peer Reviewed
• [Journal Article] Quantitation of fungal DNA contamination in commercial zymolyase and lyticase used in preparatin of fungi. (2009)
  • Author(s): Miyajima Y
  • Journal Title: Japanese Journal of Medical Mycology 50(4) Pages: 259-262
  • Peer Reviewed
• [Journal Article] Candida auris sp. nov., a novel ascomycetous yeast isolated from the_external ear canal of an inpatient in a Japanese hospital. (2009)
  • Author(s): Satoh K
  • Journal Title: Microbiology and Immunology 53(1) Pages: 41-44
  • Peer Reviewed
• [Journal Article] Typing of Aspergillus fumigatus and Aspergillus niger Strains by Random Amplification of Polymorphic DNA Analysis Using a Six Primer Set. (2009)
  • Author(s): Mirhendi H
  • Journal Title: Shiraz E-Medical Journal 10(4) Pages: 190-200
  • Peer Reviewed
• [Journal Article] Malassezia属真菌の関与が疑われた外耳道炎の検討. (2009)
  • Author(s): 塩田量子
  • Journal Title: 医真菌学会誌 50(2) Pages: 109-116
  • Peer Reviewed
• [Journal Article] nested PCR法を用いた爪白癬の菌種同定と治癒判断の試み。 (2009)
  • Author(s): 佐藤公美子
  • Journal Title: 東京医科大学雑誌 67(3) Pages: 299-305
  • Peer Reviewed
• [Journal Article] 打ち抜き感染(Breakthrough infection)として発症した侵襲性ムコール症の1剖検例. (2009)
  • Author(s): 石原晋
  • Journal Title: The Japanese Journal of Antibiotics 62(1) Pages: 55-62
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: 病原真菌の基礎知識.特集皮膚真菌症診療ガイド-これだけは知っておきたい皮膚真菌症の知識-.MB Derma 148(全日本病院出版会) Pages: 1-5
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: 宇宙:近未来のヒト生活環境と微生物(1)-真菌から見た宇宙におけるヒト生活環境-ウォストークからミールまで.The Chemical Times 211(関東化学株式会社) Pages: 22-25
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: 接合菌と接合菌症.Japanese Journal of Antibiotics 62(1)(財団法人 日本抗生物質学術協議会) Pages: 61-62
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: 治療の進歩新規抗真菌薬Annual Review呼吸器2009巻(中外医学社) Pages: 237-241
• [Journal Article] (2009)
  • Author(s): 山村麻倫子
  • Journal Title: 「呼吸器における薬剤の現状と開発」感染症抗真菌薬の現状。THE LUNG-perspectives 17(3)(メディカルレビュー社) Pages: 252-256
• [Journal Article] (2009)
  • Author(s): 小野崎正修
  • Journal Title: 薬剤感受性測定法と耐性菌 2特殊微生物の抗微生物薬感受性測定法真菌臨床と微生物36巻(増刊号)2(近代出版) Pages: 596-574
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: 深在性真菌症 起因菌からのアプローチMedical Q平成21年7月20日号(株式会社日経ラジオ社) Pages: 1-3
• [Journal Article] (2009)
  • Author(s): 川上小夜子
  • Journal Title: 真菌検査法カラーアトラスMalassezia furfurに関連した中心静脈カテーテル感染症と微生物学的検査。深在性真菌症:SFI Forum 5(1)(メディカルレビュー社) Pages: 20-23
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: 第13回クリプトコッカス症 医師から見たズーノーシス 飼い主・動物医療従事者の感染予防のためにJournal of Small Animal Medicine 11(6)(インターズー) Pages: 84-87
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: 新規抗真菌薬Annual Review呼吸器2009東京(中外医学社)(工藤翔二、土屋了介、金沢実、大田健編) Pages: 237-241
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: 真菌症の診断と治療ハリソン内科学第3版Part7感染症Section 16真菌および微細藻類感染症(メディカルサイエンスインターナショナル株式会社) Pages: 1304-1306
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: その他の真菌症と微細藻類感染症ハリソン内科学第3版Part7感染症Section 16真菌および微細藻類感染症(メディカルサイエンスインターナショナル株式会社) Pages: 1325-1330
• [Journal Article] (2009)
  • Author(s): 槇村浩一
  • Journal Title: Pneumocystis感染症ハリソン内科学第3版Part7感染症Section 16真菌および微細藻類感染症(メディカルサイエンスインターナショナル株式会社) Pages: 1330-1332
• [Journal Article] Agrobacterium tumefaciens-mediated transformation of the dermatophyte, Trichophyton mentagrophytes : an efficient tool for genetransfer (2009)
  • Author(s): Tsuyoshi Yaraada, Koichi Makimura, Kazuo Satoh, Yoshiko Umeda, Yumiko Ishihara, Shigeru Abe
  • Journal Title: Medical Mycology (印刷中)
  • Peer Reviewed
• [Journal Article] Development of loop-mediated isothermal amplification method for diagnosing Pneumocystis pneumonia. (2008)
  • Author(s): Uemura N, Makimura K, Onozaki M, Otsuka Y, Shibuya Y, Yazaki H, Kikuchi Y, Abe S, Kudoh S
  • Journal Title: Journal of Medical Microbiology 57(1) Pages: 50-7
  • NAID: 130006947885
• [Journal Article] Genetic transformation of the dermatophyte, Trichophyton mentagrophytes, based on the use of G418 resistance as a dominant selectable marker. (2008)
  • Author(s): Yamada T, Makimura K, Hisajima T, Ito M, Umeda Y, Abe S
  • Journal Title: Journal of Dermatological Science 49(1) Pages: 53-61
  • NAID: 10024118229
• [Journal Article] Exotic myiasis caused by 19 larvae of Cordylobia anthropophaga in Namibia and identified using molecular methods in Japan. (2008)
  • Author(s): Fijisaki R, Makimura K, Hayashi T, Yamamura M, Yamaoka T, Shiraishi K, Ishihbashi S, Kawakami S, Kurihara T, Nishihya H
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 102(6) Pages: 599-601
• [Journal Article] Prototheca zopfii genotypes isolated from cow barns and bovine mastitis in Japan. (2008)
  • Author(s): Osumi T, Kishimoto Y, Kano R, Maruyama H, Onozaki M, Makimura K, Ito T, Matsubara K, Hasegawa A
  • Journal Title: Veterinary Microbiolgy 131(3-4) Pages: 419-423
• [Journal Article] A new genotype of Arthroderma benhamiae. (2008)
  • Author(s): Kano R, Sano A, Makimura K, Watanabe S, Nishimura K, Yamaguchi H, Hasegawa A
  • Journal Title: Medical Mycology 46(7) Pages: 739-744
• [Journal Article] Protective effects of farnesol against oral candidiasis in mice. (2008)
  • Author(s): Hisajima T, Maruyama N, Tanabe Y, Ishibashi H, Yamada T, Makimura K, Nishiyama Y, Funakoshi K, Oshima H, Abe S
  • Journal Title: Microbiology and Immunology 52(7) Pages: 327-33
  • NAID: 10027684449
• [Journal Article] Comparative evaluation of Trichosporon asahii susceptibility using ASTY colorimetric microdilution and CLSI M27-A2 broth microdilution reference methods. (2008)
  • Author(s): Kalkanci A, Mekha N, Poonwan N, Makimura K, Sugita T
  • Journal Title: Microbiol Immunol 52(9) Pages: 435-439
  • NAID: 10025692118
• [Journal Article] Sporobolomyces koalae sp. nov., a novel basidiomycetous yeast isolated from the nasal smear of Queensland koalas kept in a Japanese zoological park (2008)
  • Author(s): Satoh K, Makimura K
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology 58(Pt12) Pages: 2983-2986
• [Journal Article] Is Histoplasma capsulatum a native inhabitant of Japan? (2008)
  • Author(s): Kikuchi K, Sugita T, Makimura K, Urata K, Someya T, Sasaki T, Kamei K, Niimi M, Hiramatsu K, Uehara Y
  • Journal Title: Microbiology and Immunology 52(9) Pages: 455-459
  • NAID: 10025692219
• [Journal Article] A new genotype of Arthroderma benhamiae. (2008)
  • Author(s): Kano R, Sano A, Makimura K, Watanabe S, Nishimura K, Yamaguchi H, Hasegawa A.
  • Journal Title: Medical Mycology Nov 46(7) Pages: 739-44
• [Journal Article] Genetic transformation of the dermatophyte, Trichophyton mentagrophytes, based on the use of G418 resistance as a dominant selectable marker (2008)
  • Author(s): Yamada T, Makimura K, Hisajima T, Ito M, Umeda Y, Abe S
  • Journal Title: Journal of Dermatological Science 49(1) Pages: 53-61
  • NAID: 10024118229
  • Peer Reviewed
• [Journal Article] Genetic transformation of the dermatophyte, Trichophyton mentagrophytes, based on the use of G418 resistance as a dominant selectable marker (2008)
  • Author(s): Tsuyoshi Yamada, Koichi Makimura, Tatsuya Hisajima Maki Ito, Yoshiko Umeda, Shigeru Abe
  • Journal Title: Journal of Dermatological Science 49 Pages: 53-61
  • NAID: 10024118229
  • Peer Reviewed
• [Journal Article] The effect of dermatophytes on cytokine production by human keratinocytes. (2007)
  • Author(s): Tani K, Adachi M, Nakamura Y, Kano R, Makimura K, Hasegawa A, Kanda N, Watanabe S.
  • Journal Title: Arch Dermatol Res 299(8) Pages: 381-7
• [Journal Article] Identification of pathogenic Aspergillus species by a PCR-restriction enzyme method. (2007)
  • Author(s): Mirhendi H, Diba K, Kordbacheh P, Jalalizand N, Makimura K
  • Journal Title: Journal of Medical Microbiology 56(11) Pages: 1568-70
• [Journal Article] Revised culture based identification system for Malassezia Journal of Clinical Microbiology (2007)
  • Author(s): Kaneko T, Makimura K, Abe M, Shiota R, Nakamura Y, Kano R, Hasegawa A, Sugita T, Shibuya S, Watanabe S, Yamaguchi H, Abe S, Okamura N
  • Journal Title: 45(11) Pages: 3737-42
• [Journal Article] The effect of dermatophytes on cytokine production by human keratinocytes (2007)
  • Author(s): Tani K, Adachi M, Nakamura Y, Kano R, Makimura K, Hasegawa A, Kanda N, Watanabe S.
  • Journal Title: Arch Dermatol Res 299 Pages: 381-387
  • Peer Reviewed
• [Journal Article] 真菌の多様性と病原性-特集2 微生物の生残戦略と病原性の関連 (2007)
  • Author(s): 愼村 浩一
  • Journal Title: 獣医畜産新報 60 Pages: 587-588
• [Journal Article] Serum Galactomannan Negative Disseminated Aspergillosis In An Intensive Care Unit; A Case Report.
  • Author(s): Saito T
  • Journal Title: Infection (in press)
  • Peer Reviewed
• [Journal Article] Prototheca cutis sp. nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin
  • Author(s): Satoh K
  • Journal Title: Int J Syst Evol Microbiol. (in press)
  • Peer Reviewed
• [Journal Article] 26S rDNA-based phylogenetic investigation of Japanese cattle-associated Prototheca zopfii isolates.
  • Author(s): Kishimoto Y
  • Journal Title: The Journal of Veterinary Medical Science (in press)
  • Peer Reviewed
• [Journal Article] Nourseothricin acetyltransferase : a new dominant selectable marker for the dermatophyte Trtchophyton mentagrophytes.
  • Author(s): Alshahni M
  • Journal Title: Medical Mycology (in press)
  • Peer Reviewed
• [Journal Article] Human External Ear Canal as the Specific Reservoir of Malassezia slooffiae.
  • Author(s): Kaneko T
  • Journal Title: Medical Mycology (in press)
  • Peer Reviewed
• [Presentation] 日常検査における酵素基質を用いた黄色ブドウ球菌(MSSA/MRSA)スクリーン培地の有用性 (2010)
  • Author(s): 菅原恵子
  • Organizer: 第21回日本臨床微生物学会総会(P-127)
  • Place of Presentation: 東京ドーム
  • Year and Date: 2010-01-31
• [Presentation] カビから見る深在性真菌症 (2010)
  • Author(s): 槇村浩一
  • Organizer: 第21回日本臨床微生物学会総会イブニングセミナー1
  • Place of Presentation: 東京ドームホテル
  • Year and Date: 2010-01-30
• [Presentation] 抗真菌化学療法 -その可能性- (2010)
  • Author(s): 槇村浩一
  • Organizer: 第2回新都心血液学セミナー(大学院特別講義)
  • Place of Presentation: 京王プラザホテル 新宿
  • Year and Date: 2010-01-21
• [Presentation] 深在性真菌症と起因菌 (2009)
  • Author(s): 槇村浩一
  • Organizer: 第119回山梨臨床検査医学研究会
  • Place of Presentation: 甲府富士屋ホテル
  • Year and Date: 2009-12-04
• [Presentation] 真菌症遺伝子診断のアップデート (2009)
  • Author(s): 槇村浩一
  • Organizer: 第三十回関東医真菌懇話会
  • Place of Presentation: ヤンセンファーマ 本社三階会議室 神田・東京
  • Year and Date: 2009-11-21
• [Presentation] 真菌症遺伝子診断のアップデート (2009)
  • Author(s): 槇村浩一
  • Organizer: 第30回関東医真菌懇話会 シンポジウム
  • Place of Presentation: ヤンセンファーマ(株)西神田
  • Year and Date: 2009-11-21
• [Presentation] 医真菌領域における菌種同定。 (2009)
  • Author(s): 槇村浩一
  • Organizer: 日本菌学会・日本防菌防黴学会合同シンポジウム
  • Place of Presentation: 東京医科歯科大学歯学部特別講堂
  • Year and Date: 2009-11-07
• [Presentation] Microbes in space (2009)
  • Author(s): 槇村浩一
  • Organizer: グルカン研究会
  • Place of Presentation: 大手町サンケイプラザ
  • Year and Date: 2009-10-13
• [Presentation] Microbe in space. (2009)
  • Author(s): Makimura, Koichi
  • Organizer: Glucan Workshop 2009.
  • Place of Presentation: Otemachi Sankei Plaza Sankei Bldg, Tokyo.
  • Year and Date: 2009-10-13
• [Presentation] Microbiological research on board International Space Station/ "KIBO" (2009)
  • Author(s): Koichi Makimura
  • Organizer: The 23^rd Annual Meeting of the Japanese Society for Biological Sciences in Space.
  • Place of Presentation: JAXA Tsukuba Space Center
  • Year and Date: 2009-10-02
• [Presentation] 病原真菌と検査法のアップデート (2009)
  • Author(s): 槇村浩一
  • Organizer: 真菌の検査法と病原性に関する研究会
  • Place of Presentation: 札幌市教育文化会館
  • Year and Date: 2009-07-17
• [Presentation] 病原真菌と健康障害のup to date. (2009)
  • Author(s): 槇村浩一
  • Organizer: 第326回八戸地区病院薬剤師会薬学例会
  • Place of Presentation: 八戸パークホテル
  • Year and Date: 2009-04-16
• [Presentation] 深在性真菌症と起因菌のアップデート (2009)
  • Author(s): 槇村浩一
  • Organizer: 重症感染症セミナー
  • Place of Presentation: 熊本大学医学部付属病院山崎記念館
  • Year and Date: 2009-04-15
• [Presentation] Lamp-mediated isothermal amplification (LAMP法)を用いて診断した熱帯熱マラリアの1例. (2009)
  • Author(s): 山村麻倫子
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 京王プラザ
• [Presentation] Phylogenic analysis and molecular detection and identification of Protothecha. (2009)
  • Author(s): Koichi Makimura
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Specific detection and identification of fungal DNA using quantitative PCR and loop-mediated isothermal amplification; their advantages and limitations. (2009)
  • Author(s): Koichi Makimura
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Rapid direct colony PCR from fungi by ampdirect plus. (2009)
  • Author(s): Alshahni M
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Real-time PCR quantitation of DNA contamimant in recent β-glucanase products used for fungal preparations (2009)
  • Author(s): Miyajima Y
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Development of Prototheca zopfii detecting system with TaqMan MGB probe and resolight dye. (2009)
  • Author(s): Onozaki M
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Clinical isolates of Aspergillus fumigatus and A. niger from transplant recipients in Japan. (2009)
  • Author(s): Moe Asano
  • Organizer: The International Society for Human and Animal Mvcoloev 2009
  • Place of Presentation: Tokyo
• [Presentation] Antifungal activity of luliconazole in a guinea pig seborrheic dermatitis model with Malassezia restricta. (2009)
  • Author(s): Yukimi Munechika
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Preliminary identification and typing of pathogenic and toxigenic fusarium species based on restriction digestion of ITS1-5.8S rDNA-ITS2 region. (2009)
  • Author(s): Hossein Mirhend
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Identification of pathogenic yeasts speies based on PCR-fragment size polymorphism (PCR-FSP) by using normal agarose gel electrophoresis. (2009)
  • Author(s): Hossein Mirhendi
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] A new species of the genus Malassezia based on the sequence analysis of 26S (D1/D2) and internal transcribed spacer 1 in Ribozomal DNA. (2009)
  • Author(s): Hossein Mirhendi
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] A newly pathogen of protothecosis as a novel achlorophyllic alga isolated from the inflamed skin caused by protothecosis. (2009)
  • Author(s): Satoh K
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Development of analyzing system for microbial flora on board International Space Station and astronauts. (2009)
  • Author(s): Makimura K
  • Organizer: The International Society for Human and Animal Mcology 2009
  • Place of Presentation: Tokyo
• [Presentation] Enhanced gene replacements in Ku80 disruption metants of the dermatophyte, Trichophyton mentagrophytes. (2009)
  • Author(s): Tsuyoshi Yamada
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Detection of dermatophytes and nondermatophytes from onychomycosis patients after systemic antifungal treatment : Evaluation of cure by nested PCR. (2009)
  • Author(s): Mayuko Muro
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Karyotype diffrence of the Czech and Japanese Candida glabrata bloodstream isolates. (2009)
  • Author(s): Petr Hamal
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] Acase of onychomycosis in a Japanese monkey. (2009)
  • Author(s): Alshahni M
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] In vitro susceptibility of Prothothotheca Zopfil genotype 1 and Z (2009)
  • Author(s): Hideto Sobukawa
  • Organizer: The International Society for Human and Animal Mycology 2009
  • Place of Presentation: Tokyo
• [Presentation] 長期ステロイド剤外用後に生じた皮膚フサリウム症の一例 (2009)
  • Author(s): 山田陽三
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡
• [Presentation] アグロバクテリウム法を用いた白癬菌遺伝子破壊株の作出 (2009)
  • Author(s): 山田 剛, 槇村浩一, 安部 茂
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] アグロバクテリウム法を用いた白癖菌遺伝子破壊株の作出について (2008)
  • Author(s): 山田 剛, 槇村浩一, 安部 茂
  • Organizer: 第52回日本医真菌学会総会
  • Place of Presentation: 長崎大学
• [Presentation] アグロバクテリウム法を用いた白癬菌形質転換体作出の試み (2008)
  • Author(s): 山田 剛, 愼村 浩一, 安部 茂
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 国立京都国際会館、京都
• [Presentation] Development of Analyzing System for Microbial Flora on board Space Station and Astronauts. (2007)
  • Author(s): Koichi MAKIMURA, Kazuo SATOH, Tsuyoshi YAMADA, Yayoi NISHIYAMA, Shigeru ABE, Yuuji TSUKII, Takashi SUGITA, Kosuke TAKATORI, Yoshimi BENNO, Takashi YAMAZAKI
  • Organizer: The 5th ISLSWG International Workshop on Space Microbiology
  • Place of Presentation: Tokyo
• [Book] 真菌症の診断と治療ハリソン内科学第3版Part7感染症Section 16真菌および微細藻類感染症 (2009)
  • Author(s): 槇村浩一
• [Book] 水虫最前線 皮膚科診療最前線シリーズ (2007)
  • Author(s): 愼村 浩一(共著)
  • Total Pages: 274
  • Publisher: メディカルレビュー社
• [Remarks] 帝京大学医真菌研究センターウェブサイト
  • URL: http://timm.main.teikyo-u.ac.jp
• [Remarks] 病原真菌データーベース
  • URL: http://www.pfdb.net/
• [Remarks] 1.帝京大学医真菌研究センター ウェブサイト
  • URL: http://timm.teikyo-u.ac.jp
• [Remarks] 2.病原真菌データーベース
  • URL: http://www.pfdb.net/
• [Remarks] 帝京大学医真菌研究センター ウェブサイト
  • URL: http://timm.main.teikyo-u.ac.jp
• [Remarks] 病原真菌データベース
  • URL: http://www.pfdb.net/
• [Remarks] 帝京大学医真菌研究センター ウェブサイト
  • URL: http://timm.main.teikyo-u.ac.jp
• [Remarks] 病原真菌データベース
  • URL: http://www.pfdb.net/
• [Patent(Industrial Property Rights)] 遺伝子破壊白癬菌 (2008)
  • Inventor(s): 槇村浩一、山田剛、安部茂
  • Industrial Property Rights Holder: 槇村浩一、山田剛、安部茂
  • Industrial Property Number: 2008-011432
  • Filing Date: 2008-04-22
• [Patent(Industrial Property Rights)] 爪真菌症の診断法 (2008)
  • Inventor(s): 坪井良治、槇村浩一、海老原睦仁
  • Industrial Property Rights Holder: 坪井良治、槇村浩一、海老原睦仁
  • Industrial Property Number: 2008-067605
  • Filing Date: 2008-03-27