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Investigation of mechanism of paramyxovirus budding with accessory proteins by using vi rus reconstitution systems

Research Project

Project/Area Number 19590475
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionHiroshima University

Principal Investigator

SAKAGUCHI Takemasa  Hiroshima University, 大学院・医歯薬学総合研究科, 准教授 (70196070)

Co-Investigator(Kenkyū-buntansha) IRIE Takashi  広島大学, 大学院・医歯薬学総合研究科, 助教 (70419498)
Research Collaborator KADOI Takahiro  広島大学, 大学院・医歯薬学総合研究科, 大学院生
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsパラミクソウイルス / センダイウイルス / ニパウイルス / ウイルス出芽 / C蛋白質 / cDNAからのウイルス作製系 / ウイルス / cDNAからのウイルス作成系
Research Abstract

多くの病原ウイルスを含むパラミクソウイルスの粒子形成過程(出芽)を研究した。cDNAからウイルスを人工合成して、M 蛋白質と宿主因子Alixとの結合がウイルス出芽に重要であることを示した。一方、アクセサリー蛋白質CとAlixとの結合は出芽に影響を及ぼさなかったが、多くのC変異ウイルスの解析から抗インターフェロン能の詳細を示し、さらにC蛋白質がRNA合成を制御してウイルスゲノム極性を決めていることを解明した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (35 results)

All 2009 2008 2007

All Journal Article (12 results) (of which Peer Reviewed: 9 results) Presentation (23 results)

  • [Journal Article] Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus -mouse system2008

    • Author(s)
      Sakaguchi, T., Kato, A., Kiyotani, K., Yoshida, T., and Nagai, Y
    • Journal Title

      Proc. Jpn. Acad. Ser B 84

      Pages: 439-451

    • NAID

      130000093948

    • Related Report
      2008 Final Research Report
  • [Journal Article] Paramyxovirus Sendai virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles2008

    • Author(s)
      Irie, T., Nagata, N., Yoshida, T., and Sakaguchi, T.
    • Journal Title

      Virology 374

      Pages: 495-505

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of the leader sequence to homologous viral interference among Sendai virus strains2008

    • Author(s)
      Shimazu, Y., Takao, S., Irie, T., Kiyotani, K., Yoshida, T., and Sakaguchi, T.
    • Journal Title

      Virology 372

      Pages: 64-71

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recruitment of Alix/AIP1 to the plasma membrane by the Sendai virus C protein facilitates budding of virus -like particles2008

    • Author(s)
      Irie, T., Nagata, N., Yoshida, T., and Sakaguchi, T.
    • Journal Title

      Virology 371

      Pages: 108-120

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Paramyxovirus Sendai virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles.2008

    • Author(s)
      入江 崇
    • Journal Title

      Virology 374

      Pages: 495-505

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus-mouse system.2008

    • Author(s)
      坂口 剛正
    • Journal Title

      Proc. Jpn. Acad., Ser. B 84

      Pages: 495-505

    • NAID

      130000093948

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recruitment of Alix/AIP1 to the plasma membrane by the Sendai virus C protein facilitates budding of virus-like particles.2008

    • Author(s)
      Irie, T.
    • Journal Title

      Virology 371

      Pages: 108-120

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of the leader sequence to homologous viral interference among Sendai virus strains.2008

    • Author(s)
      Shimazu, Y.
    • Journal Title

      Virology 372

      Pages: 64-71

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Persistent and stable gene expression by a cytoplasmic RNA replicon based on a noncytopathic variant Sendai virus2007

    • Author(s)
      Nishimura, K., Segawa, H., Goto, T., Morishita, M., Masago, A., Takahashi, H., Ohmiya, Y., Sakaguchi, T., Asada, M., Imamura, T., Shimotono, K., Takayama, K., Yoshida, T., and Nakanishi, M.
    • Journal Title

      J. Biol. Chem 282

      Pages: 27383-27391

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] パラミクソウイルスの出芽機構2007

    • Author(s)
      入江崇、坂口剛正
    • Journal Title

      ウイルス 57

      Pages: 1-8

    • NAID

      10019530623

    • Related Report
      2008 Final Research Report
  • [Journal Article] パラミクソウイルスの出芽機構2007

    • Author(s)
      入江 崇
    • Journal Title

      ウイルス 57

      Pages: 1-8

    • NAID

      10019530623

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Persistent and stable gene expression by a cytoplasmic RNA replicon based on a noncytopathic variant Sendai virus.2007

    • Author(s)
      Nishimura, K.
    • Journal Title

      Journal of the Biological Chemistry 282

      Pages: 27383-27391

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Cellular machinery for enveloped virus budding. 1stsymposium of optical surgery (Supported by CREST)2009

    • Author(s)
      坂口剛正
    • Organizer
      Roles of Host Factors in Viral Replication
    • Place of Presentation
      法政大学ボアソナード・タワー(東京都)
    • Year and Date
      2009-02-28
    • Related Report
      2008 Final Research Report
  • [Presentation] ウイルス感染における宿主因子の役割2009

    • Author(s)
      坂口 剛正
    • Organizer
      1st symposium of optical surgery (by CREST) Roles of Host Factors in Viral Replication
    • Place of Presentation
      法政大学ボアソナード・タワー(東京都)
    • Year and Date
      2009-02-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] センダイウイルスM蛋白質YLDL配列の重要性2008

    • Author(s)
      坂口剛正
    • Organizer
      第56回日本ウイルス学会学術集会
    • Place of Presentation
      岡山コンベンションセンター(岡山市)
    • Year and Date
      2008-10-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] センダイウイルスC蛋白質の自然免疫応答に対する機能的多様性2008

    • Author(s)
      入江崇
    • Organizer
      第56回日本ウイルス学会学術集会
    • Place of Presentation
      岡山コンベンションセンター(岡山市)
    • Year and Date
      2008-10-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Paramyxovirus Sendai virus C proteins are essential for maintenance of negative -sense RNA genome in virus particles2008

    • Author(s)
      入江崇
    • Organizer
      第8回あわじしま感染症免疫フォーラム
    • Place of Presentation
      兵庫県立淡路夢舞台国際会議場(淡路市)
    • Year and Date
      2008-09-07
    • Related Report
      2008 Final Research Report
  • [Presentation] Paramyxovirus Sendai virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles.2008

    • Author(s)
      入江 崇
    • Organizer
      第8回あわじしま感染症免疫フォーラム
    • Place of Presentation
      淡路夢舞台国際会議場(淡路市)
    • Year and Date
      2008-09-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] Paramyxovirus Sendai virus C proteins are essential for maintenance of negative-sense RNA genome In virus particles.2008

    • Author(s)
      入江 崇
    • Organizer
      XIVth International Congress of Virology
    • Place of Presentation
      イスタンブール(トルコ)
    • Year and Date
      2008-08-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Paramyxovirus Sendai virus C proteins are essential for maintenance of negative -sense RNA genome in virus particles2008

    • Author(s)
      入江崇
    • Organizer
      第14回国際ウイルス学会
    • Place of Presentation
      イスタンブール(トルコ共和国)
    • Year and Date
      2008-08-10
    • Related Report
      2008 Final Research Report
  • [Presentation] ニパウイルスM蛋白質のリン酸化が及ぼす出芽への影響2007

    • Author(s)
      門井 喬浩
    • Organizer
      第55回日本ウイルス学会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2007-10-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] センダイウイルスC蛋白質によるM-VLP出芽促進機構の解明2007

    • Author(s)
      坂口 剛正
    • Organizer
      第55回日本ウイルス学会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2007-10-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] センダイウイルスの複製におけるC蛋白質の役割の再考2007

    • Author(s)
      入江 崇
    • Organizer
      第55回日本ウイルス学会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2007-10-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] センダイウイルスC蛋白質によるM-VLP出芽促進機構の解明2007

    • Author(s)
      坂口剛正
    • Organizer
      第55回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2007-10-21
    • Related Report
      2008 Final Research Report
  • [Presentation] センダイウイルスの複製におけるC蛋白質の役割の再考2007

    • Author(s)
      入江崇
    • Organizer
      第55回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2007-10-21
    • Related Report
      2008 Final Research Report
  • [Presentation] ニパウイルスM蛋白質のリン酸化が及ぼす出芽への影響2007

    • Author(s)
      門井喬浩
    • Organizer
      第55回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2007-10-21
    • Related Report
      2008 Final Research Report
  • [Presentation] Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles2007

    • Author(s)
      Sakaguchi, T.
    • Organizer
      Negative strand RNA viruses 2007 Workshop
    • Place of Presentation
      Northwestern University (Evanston, U.S.A.)
    • Year and Date
      2007-09-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles2007

    • Author(s)
      坂口剛正
    • Organizer
      Negative Strand RNA Viruses 2007 Workshop
    • Place of Presentation
      エバンストン(アメリカ合衆国)
    • Year and Date
      2007-09-15
    • Related Report
      2008 Final Research Report
  • [Presentation] Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles2007

    • Author(s)
      Irie, T.
    • Organizer
      第7回あわじしま感染症免疫フォーラム
    • Place of Presentation
      淡路夢舞台国際会議場(淡路市)
    • Year and Date
      2007-09-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles2007

    • Author(s)
      入江崇
    • Organizer
      第7回あわじしま感染症免疫フォーラム
    • Place of Presentation
      兵庫県立淡路夢舞台国際会議場(淡路市)
    • Year and Date
      2007-09-01
    • Related Report
      2008 Final Research Report
  • [Presentation] センダイウイルスの出芽機構2007

    • Author(s)
      坂口剛正
    • Organizer
      特定領域研究A03班会議
    • Place of Presentation
      九州大学総合研究棟(福岡市)
    • Year and Date
      2007-07-17
    • Related Report
      2008 Final Research Report
  • [Presentation] センダイウイルスの出芽機構2007

    • Author(s)
      坂口 剛正
    • Organizer
      特定領域研究「感染のマトリクス」A03班会議
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2007-07-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] センダイウイルスC蛋白質による出芽促進機構の解明2007

    • Author(s)
      入江 崇
    • Organizer
      第23回中国四国ウイルス研究会
    • Place of Presentation
      松山市
    • Year and Date
      2007-06-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] センダイウイルスC蛋白質による出芽促進機構の解明2007

    • Author(s)
      入江崇
    • Organizer
      第23回中国四国ウイルス研究会
    • Place of Presentation
      にぎたつ会館(松山市)
    • Year and Date
      2007-06-16
    • Related Report
      2008 Final Research Report
  • [Presentation] ニパウイルスM蛋白質のリン酸化が及ぼす出芽への影響2007

    • Author(s)
      門井喬浩
    • Organizer
      第23回中国四国ウイルス研究会
    • Place of Presentation
      にぎたつ会館(松山市)
    • Year and Date
      2007-06-16
    • Related Report
      2008 Final Research Report 2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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