Basic analysis on the mechanism of antigenic change in influenza B viruses using bioinformatics

• Project/Area Number: 19590482
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Nagoya City University
• Principal Investigator: NOBUSAWA Eri Nagoya City University, 大学院医学研究科, 准教授 (90183904)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: インフルエンザウイルス / 血球凝集素 / 変異許容性

Research Abstract

ランダム変異導入法によりB 型インフルエンザウイルス(B/Kanagwa/73)HA の一アミノ酸変異HA を作製した。301 変異HA のうち69%の変異が許容され、これはA 型ウイルスHA の約1.5 倍に相当する。2 系統に分岐後の B 型ウイルスHA(BHA)も同程度の許容性を示した。また、分岐前後BHA 上の同一変異の96%でその影響が一致した。BHA はアミノ酸変異に対する許容性が高く変異の蓄積による構造的変化が少ないため、分岐前後のBHA 間でアミノ酸変異の機能への影響にほぼ差がないことが示唆された。

Research Products

• [Journal Article] Effects of single-point amino acid substitutions on the structure and function neuraminidase proteins in influenza A virus (2008)
  • Author(s): Yano T, Nobusawa E , Nagy A, Nakajima S, Nakajima K
  • Journal Title: Microbiol Immunol 52(4) Pages: 216-223
  • NAID: 10025691767
  • Peer Reviewed
• [Journal Article] Effects of single-point amino acid substitutions on thestructure and function neuraminidase proteins in influ-enza A virus (2008)
  • Author(s): Yano, T
  • Journal Title: Microviology and immunology 52 Pages: 55-63
  • Peer Reviewed
• [Journal Article] Comparison of epitope structures of H3HAs through protein modeling of influenza A virus hemagglutinin: mechanism for selection of antigenic variants in the presence of a monoclonal antibody (2007)
  • Author(s): Nakajima S, Nakajima K, Nobusawa E, Zhao J, Tanaka S, Fukuzawa K
  • Journal Title: Microbiol Immunol 51(12) Pages: 1179-1187
  • NAID: 10020278027
  • Peer Reviewed
• [Journal Article] Comparison of epitope structures of H3HAs through protein modeling of influenza A virus hemagglutinin: mechanism for selection of antigenic variants in the presence of a monoclonal antibody. (2007)
  • Author(s): Nakajima S
  • Journal Title: Microbiology and immunology 51 Pages: 1179-1187
  • Peer Reviewed
• [Presentation] B 型インフルエンザウイルスHA 上のアミノ酸変異の蓄積 (2008)
  • Author(s): 信澤枝里
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2008-10-26
• [Presentation] B型インフルエンザウイルスHA上のアミノ酸変異の蓄積 (2008)
  • Author(s): 信澤枝里
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2008-10-26
• [Presentation] The Tolerance of Influenza B Virus HA To Amino Acid Changes (2008)
  • Author(s): Nobusawa Eri
  • Organizer: Options for the Control of Influenza VI
  • Place of Presentation: Metro Toronto Convention Centre, Toronto, Canada
  • Year and Date: 2008-06-18