Research on EB virus infection cell control that makes CD40 target

• Project/Area Number: 19590486
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: IMADOME Kenichi National Research Institute for Child Health and Development, 母児感染研究部, 共同研究員 (70392488)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: CD40 / EBV / EBウイルス / CD40リガンド

Research Abstract

上皮細胞へEBウイルス(EBV)が感染することでCD40リガンド(CD40L)が発現誘導され、上皮細胞上に存在するCD40と相互作用をすることでアポトーシス回避、細胞増殖に働いていることが示された。また、このCD40シグナルを阻害すると感染細胞のアポトーシス回避、細胞増殖が抑制された。これにより、EBV感染可能な細胞の全て(上皮細胞、B,T,NK細胞)でCD40シグナルが感染細胞の標的分子となりうることが明らかとなった。

Research Products

• [Journal Article] CD40 signaling activated by Epstein-Barr virus promotes cell survival and proliferation in gastric carcinoma-drived human epithelial cells Microbes & Infection (2009)
  • Author(s): Imadome K., et al.
  • Journal Title: 11 Pages: 429-433
  • Peer Reviewed
• [Journal Article] CD40 signaling activated by Epstein-Barr virus promotes cell survival and proliferation in gastric carcinoma-derived human epithelial cells (2009)
  • Author(s): Imadome K., et al
  • Journal Title: Microbes and Infection 11 Pages: 429-433
  • Peer Reviewed
• [Journal Article] A new humanized mouse model of Epstein-Barr virus infection that reproduces persistent infection, lymphoproliferative disorder, and cell-mediated and humoral immune responses (2008)
  • Author(s): Imadome K., et al.
  • Journal Title: J.Infect.Dis 198 Pages: 673-682
  • Peer Reviewed
• [Journal Article] The Latent Membrane Protein 1 (LMP1) Encoded by Epstein-Barr Virus Induces Expression of the Putative Oncogene Bcl-3 Through Activation of the Nuclear Factor-kB. (2008)
  • Author(s): Nakamura H, Iguchi A, Kuroda K, Shimizu K, Shimizu N, Imadome K.,et al.
  • Journal Title: Virus Res 131 Pages: 170-179
  • Peer Reviewed
• [Journal Article] A new humanized mouse model of EBV infection reproducing persistent infection, lymphoproliferative disorder, and cell-mediated and humoral immune responses (2008)
  • Author(s): Imadome K., et al.
  • Journal Title: Journal of Infectious diseases 198 Pages: 673-682
  • Peer Reviewed
• [Journal Article] The latent membrane protein 1 (LMP1) encoded by Epstein-Barr virus in duces expression of the putative oncogene Bcl-3 through activation of the nuclear factor-kappaB (2008)
  • Author(s): Nakamura H., Imadome K., et al
  • Journal Title: Virus Research 131 Pages: 170-179
  • Peer Reviewed
• [Journal Article] A new humanized mouse model of EBV infection reproducing persistent infection,lymphop roliferative disorder,and cell-mediated and humoral immune responses
  • Author(s): Ken-Ichi Imadome, et. al.
  • Journal Title: Journal of Infectious Disease (in press)
  • Peer Reviewed
• [Presentation] NOGマウスを用いた慢性活動性EBウイルス感染症モデルの作製 (2008)
  • Author(s): 今留謙一
  • Organizer: 第5回EBウイルス研究会
  • Place of Presentation: 鳥取
• [Presentation] NOGマウスを用いたCAEBVモデルマウスの作製 (2008)
  • Author(s): 今留謙一
  • Organizer: 第5回EBウイルス研究会
  • Place of Presentation: 鳥取米子
• [Presentation] EBウイルス感染上皮細胞におけるCD40シグナルの働き (2007)
  • Author(s): 今留 謙一
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] 異所性CD40シグナルを標的としたEBウイルス感染細胞制御とヒト化NOGマウスを用いた新規薬剤の開発 (2007)
  • Organizer: 第7回小児リウマチ研究会
  • Place of Presentation: 東京
• [Presentation] Epstein-Barr virus specific immune response in the humanized NOG mouse model (2007)
  • Organizer: 第37回日本免疫学会
  • Place of Presentation: 東京
• [Presentation] ヒト化NOGマウスモデルにおけるEBウイルス特異的免疫応答 (2007)
  • Organizer: 第55回日本ウイルス学会
  • Place of Presentation: 札幌
• [Presentation] EBウイルス感染上皮細胞におけるCD40シグナルの働き (2007)
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜