Identification and isolation of a new common progenitor for dendritic cell subsets

• Project/Area Number: 19590490
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Akita University
• Principal Investigator: ONAI Nobuyuki Akita University, 医学部, 講師 (50323605)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞 / 免疫学 / 樹状細胞 / 血液前駆細胞 / サイトカイン / 細胞分化・増殖

Research Abstract

樹状細胞は全身に分布し、異なる機能を持つ様々なサブセットが存在する。しかし、その分化起源は長い間不明であった。研究代表者はマウス骨髄から、樹状細胞サブセットのみに分化する共通樹状細胞前駆細胞を同定・純化した。この結果により、異なる樹状細胞サブセットが同一の前駆細胞から由来することが証明された。この発見は血液細胞分化経路図に樹状細胞分化経路を新たに追加することが出来た大変重要な研究成果である。

Research Products

• [Journal Article] Interferon regulatory factor-2 protects quiescent hematopoietic stem cells from type-I interferon- dependent exhaustion (2009)
  • Author(s): Sato, T., Onai, N., Yoshihara, H., Arai, F., Suda, T., and Ohteki, T.
  • Journal Title: Nat. Med. doi : 10.1038(in press)
  • Peer Reviewed
• [Journal Article] The concerted action of GM-CSF and Flt3-ligand on in vivo DC homeostasis (2009)
  • Author(s): Kingston, D., Schmid, M.A., Onai, N., Obata-Onai, A., Baumjohann, D., and Manz, M.G.
  • Journal Title: Blood (in press)
  • Peer Reviewed
• [Journal Article] The concerted action of GM-CSF and Flt3-ligand on in vivo DC homeostasis (2009)
  • Author(s): Kingston, D.
  • Journal Title: Blood (in press)
  • Peer Reviewed
• [Journal Article] IRF-2 protects quiescent HSCs from type-I interferon-dependent exhaustion (2009)
  • Author(s): Sato, T.
  • Journal Title: Nature Medicine (in press)
  • Peer Reviewed
• [Journal Article] The STATs on dendritic cell development (2008)
  • Author(s): Onai, N., and Manz, M.G.
  • Journal Title: Immunity 28 Pages: 490-492
  • Peer Reviewed
• [Journal Article] 樹状細胞による (2008)
  • Author(s): 小内伸幸、樗木俊聡
  • Journal Title: Th分化制御、分子細胞治療 7 Pages: 58-66
• [Journal Article] 血液前駆細胞から樹状細胞サブセットへの分化経路 (2008)
  • Author(s): 小内伸幸、小内亜矢
  • Journal Title: 実験医学 26 Pages: 3149-3157
• [Journal Article] The STATs on dendritic cell development (2008)
  • Author(s): Onai, N.
  • Journal Title: Immunity 28 Pages: 490-492
  • Peer Reviewed
• [Journal Article] 樹状細胞によるTh分化制御 (2008)
  • Author(s): 小内伸幸
  • Journal Title: 分子細胞治療 7 Pages: 58-66
• [Journal Article] 血液前駆細胞から樹状細胞サブセットへの分化経路 (2008)
  • Author(s): 小内伸幸
  • Journal Title: 実験医学増刊号 26 Pages: 3149-3157
• [Journal Article] Identification of clonogenic common Flt3^+M-CSFR^+plasmacytoid and dendritic cell progenitors in mouse bone marrow (2007)
  • Author(s): Onail, N., Obata-Onai1, A., Schmid, M.A., Ohteki, T., Jarrossay, D., and Manz, M.G.
  • Journal Title: Nat. Immunol. 8 Pages: 1207-1216
  • Peer Reviewed
• [Journal Article] Flt3 in regulation of type-I interferon producing and dendritic cell development (2007)
  • Author(s): Onai, N., Obata-Onai, A., Schmid, M. and Manz, MG.
  • Journal Title: Ann. N. Y. Acad. Sci. 1106 Pages: 253-261
  • Peer Reviewed
• [Journal Article] 樹状細胞サブセットの分化経路 (2007)
  • Author(s): 小内伸幸、小内亜矢、樗木俊聡
  • Journal Title: 生化学 79 Pages: 1143-1148
  • NAID: 10020284334
• [Journal Article] Identification of clonogenic common Flt3^+M-CSFR^+ plasmacytoid and dendritic cell progenitors in mouse bone marrow. (2007)
  • Author(s): Onai, N.
  • Journal Title: Nat. Immunol. 8 Pages: 1207-1216
  • Peer Reviewed
• [Journal Article] Flt3 in regulation of type-I interferon producing and dendritic cell development. (2007)
  • Author(s): Onai, N.
  • Journal Title: Ann. N.Y. Acad. Sci. 1106 Pages: 253-261
  • Peer Reviewed
• [Journal Article] 樹状細胞サブセットの分化経路 (2007)
  • Author(s): 小内伸幸
  • Journal Title: 生化学 79 Pages: 1143-1148
  • NAID: 10020284334
• [Presentation] 造血肝細胞の機能維持における転写因子IRF2の役割 (2008)
  • Author(s): 佐藤卓
  • Organizer: 第6回北東北血液研究会
  • Place of Presentation: 秋田
  • Year and Date: 2008-06-28
• [Presentation] The role of hematopoietic cytokine in dendritic cell subsets development (2008)
  • Author(s): Onai, N.
  • Organizer: 第38日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] Regulation of dendritic cell homeostasis (2008)
  • Author(s): Onai, N.
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: 神戸
• [Presentation] Regulation of dendritic cell homeostasis (2008)
  • Author(s): Onai, N.
  • Organizer: The 10th International Symposium on Dendritic Cells(招待講演)
  • Place of Presentation: Kobe, Japan
• [Presentation] Critical roles for Nod-like receptor in dendritic cell-mediated cross-priming induction in vivo (2008)
  • Author(s): Asano, J.
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: Kobe, Japan
• [Presentation] The role of hematopoietic cytokine in dendritic cell subsets development (2008)
  • Author(s): Onai, N.
  • Organizer: 第38日本免疫学会学術集会
  • Place of Presentation: 京都
• [Presentation] IRF-2 protects haematopoietic stem cells from type-I interferon-dependent replicative exhaustion (2008)
  • Author(s): Sato, T.
  • Organizer: 第38日本免疫学会学術集会
  • Place of Presentation: 京都
• [Presentation] Critical roles for Nod-like receptor in dendritic cell-mediated cross-priming induction in vivo (2008)
  • Author(s): Asano, J.
  • Organizer: 第38日本免疫学会学術集会
  • Place of Presentation: 京都
• [Presentation] Identification of clonogenic common plasmacytoid and dendritic cell progenitors in mouse bone marrow (2007)
  • Author(s): Onai, N.
  • Organizer: 第37回日本免疫学会総会・学術集会Late breaking symposium
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] Identification of clonogenic common plasmacytoid and dendritic cell progenitors in mouse bone marrow. (2007)
  • Author(s): Onai, N.
  • Organizer: 第37回日本免疫学会 Late breaking symposium
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] Identification of clonogenic common plasmacytoid and dendritic cell progenitors in mouse bone marrow. (2007)
  • Author(s): Onai, N.
  • Organizer: 第37回日本免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-21
• [Presentation] pDC-derived interferon-α downregulates TLR7-mediated immune responses through DC apoptosis. (2007)
  • Author(s): Kuwajima, S.
  • Organizer: 第37回日本免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-21
• [Presentation] マウス骨髄における樹状細胞前駆細胞の同定 (2007)
  • Author(s): 小内伸幸
  • Organizer: 群馬大学・秋田大学連携グローバルCOEプログラム「生体調節シグナルの統合的研究」第2回若手ミニシンポジウム
  • Place of Presentation: 秋田
  • Year and Date: 2007-11-03
• [Presentation] マウス骨髄における樹状細胞前駆細胞の同定 (2007)
  • Author(s): 小内伸幸
  • Organizer: 第2回群馬大学・秋田大学連携グローバルCOEプログラム「生体調節シグナルの統合的研究」第一回若手ミニシンポジウム
  • Place of Presentation: 秋田
  • Year and Date: 2007-11-03
• [Presentation] 樹状細胞分化におけるFlt3シグナル伝達系の役割 (2007)
  • Author(s): 小内伸幸
  • Organizer: 第5回北東北血液研究会
  • Place of Presentation: 秋田
  • Year and Date: 2007-05-12
• [Presentation] common plasmacytoid and dendritic progenitors in mouse bone marrow (2007)
  • Author(s): Onai, N.
  • Organizer: 第20回内藤コンファレンス「自然免役の医学・生物学(III)
  • Place of Presentation: 神奈川
• [Presentation] Prospective isolation of clonogenic common plasmacytoid and dendritic progenitors in mouse bone marrow. (2007)
  • Author(s): Onai, N.
  • Organizer: 第20回内藤コンファレンス「自然免役の医学・生物学(III)」
  • Place of Presentation: 葉山
• [Presentation] Plasmacytoid dendritic cell-derived interferon-α regulates dendritic cell homeostasis. (2007)
  • Author(s): Kuwajima, S.
  • Organizer: 第7回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路
• [Remarks] 東邦大学理学部生物分子科学科第13回(平成20年)生物分子科学賞受賞者 : 小内伸幸研究題目 : 樹状細胞分化メカニズムの解明
  • URL: http://www.med.akita-u.ac.jp/~kisei/Default.html
• [Remarks]
  • URL: http://www.med.akita-u.ac.jp/~kisei/Default.html
• [Remarks]
  • URL: http://www.med.akita-u.ac.jp/~kisei/Default.html