| Project/Area Number |
19590541
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
UEDA Shinichiro University of the Ryukyus, 大学院・医学研究科, 教授 (80285105)
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| Co-Investigator(Kenkyū-buntansha) |
大屋 祐輔 琉球大学, 大学院・医学研究科, 准教授 (30240964)
安 隆則 琉球大学, 大学院・医学研究科, 准教授 (40265278)
浦田 秀則 福岡大学, 医学部, 教授 (30289524)
松岡 秀洋 久留米大学, 医学部, 准教授 (80248393)
山川 研 琉球大学, 大学院・医学研究科, 助教 (00363664)
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| Co-Investigator(Renkei-kenkyūsha) |
OHYA Yusuke 琉球大学, 大学院・医学研究科, 教授 (30240964)
YASU Takanori 琉球大学, 大学院・医学研究科, 准教授 (40265278)
URATA Hidenori 福岡大学, 筑紫病院, 教授 (30289524)
MATSUOKA Hidehiro 久留米大学, 医学部, 准教授 (80248393)
YAMAKAWA Ken 琉球大学, 大学院・医学研究科, 助教 (00363664)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 心血管薬 / 薬効評価システム / サロゲートマーカー / レニンアンジオテンシン系 / ヒト内皮機能 / 代替エンドポイント / FMD / アディポネクチン / 血管内皮機能 / インスリン感受性 |
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| Research Abstract |
1. We assessed methods of the measurement of endothelial function by flow-mediated vasodilatation and of aortic stiffness by pulse wave velocity. We also standardized the method of flow-mediated vasodilatation, which had varied among researchers.
2. We estimated the association of insulin sensitivity measured by glucose clamp technique and serum metabolic marker such as adiponectin.
3. The methods of experiments in humans for the assessment of effects of elevated free fatty acids on angiotensin II forming activity in leukocytes, endothelial function, insulin sensitivity, and leukocyte activation was established. We tested effects of several cardiovascular drugs in this lipid loading experiment mimicking visceral fat obesity. We found that elevated FFA caused endothelial dysfunction, microcirculatory dysfunction (leukocyte activation), insulin resistance partly via leukocyte renin-angiotensin system. This experimental protocol may be useful for the assessment of anti-atherosclerotic drugs. Leukocyte angiotensin II forming activity could be a biomarker for visceral fat obesity linking to vascular dysfunction.
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