Molecular diagnosis by evaluating histone methylation

• Project/Area Number: 19590566
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Sapporo Medical University
• Principal Investigator: ADACHI Masaaki Sapporo Medical University, 医学部, 准教授 (70240926)
• Co-Investigator(Kenkyū-buntansha): YASUI Hiroshi 札幌医科大学, 医学部, 研究員 (40448593) ; IMAI Imai 札幌医科大学, 学長 (60117603)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ヒストンメチル化 / 小胞体ストレス / 酸化ストレス / クロマチン構造 / SUVH20 / マイクロRNA / ヒストンアセチル化 / 分子診断

Research Abstract

ヒストンメチレーションがストレス状態によって変化しうることが明らかとなった。フローサイトメトリーを用いたリンパ球の解析から、ホストのストレス状態を把握できる可能性があり、診断法開発への基盤となる成果が得られたといえる。

Research Products

• [Journal Article] Preventing oxidative stress: A new role for XBP1 (2009)
  • Author(s): Liu Y, Adachi M, Zhao S, Hareyama M, Koong AC, Luo D, Rando TA, Imai K and Shinomura Y
  • Journal Title: Cell Death Diff (in press)
  • Peer Reviewed
• [Journal Article] A novel combination therapy of arsenic trioxide with parthenolide against pancreatic cancer Cells (2009)
  • Author(s): Wang W, Adachi M, Zhou J, and Zhu D
  • Journal Title: Pancreas (in press)
  • Peer Reviewed
• [Journal Article] SNX-2112, a selective Hsp90 inhibitor, potently inhibits tumor cell growth, angiogenesis, and osteoclastogenesis in multiple myeloma and other hematologic tumors by abrogating signaling via Akt and ERK (2009)
  • Author(s): Okawa Y, Hideshima T, Steed P, Vallet S, Hall S, Huang K, Rice J, Barabasz A, Foley B, Ikeda H, Raje N, Kiziltepe T, Yasui H, Enatsu S, Anderson KC
  • Journal Title: Blood 113 Pages: 846-855
  • Peer Reviewed
• [Journal Article] Preventing oxidative stress : a new role for XBP1 (2009)
  • Author(s): Y. Liu
  • Journal Title: Cell Death Differ (In press)
  • Peer Reviewed
• [Journal Article] A novel combination therapy of arsenic trioxide with parthenolide against pancreatic cancer cells. (2009)
  • Author(s): W. Wang
  • Journal Title: Pancreas (In press)
  • Peer Reviewed
• [Journal Article] SNX-2112, a selective Hsp90 inhibitor, potently inhibits tumor cell growth, angiogenesis, and osteoclastogenesis in multiple myeloma and other hematologic tumors by abrogating signaling via Akt and ERK. (2009)
  • Author(s): Y. Okawa
  • Journal Title: Blood 113 Pages: 846-855
  • Peer Reviewed
• [Journal Article] Fatty acid synthase is a novel therapeutic target in multiple myeloma (2008)
  • Author(s): Okawa Y, Hideshima T, Ikeda H, Raje N, Vallet S, Kiziltepe T, Yasui H, Enatsu S, Pozzi S, Breitkreutz I, Cirstea D, Santo L, Richardson P, Anderson KC.
  • Journal Title: Br. J. Haematol 141 Pages: 659-671
  • Peer Reviewed
• [Journal Article] Biological pathways and in vivo antitumor activity induced by Atiprimod in myeloma (2007)
  • Author(s): Neri P, Tassone P, Shammas M, Yasui H, Schipani E, Batchu RB, Blotta S, Prabhala R, Catley L, Hamasaki M, Hideshima T, Chauhan D, Jacob GS, Picker D, Venuta S, Anderson KC, Munshi NC
  • Journal Title: Leukemia 21 Pages: 2519-2526
  • Peer Reviewed
• [Journal Article] Inhibition of Akt induces significant downregulation of survivin and cytotoxicity in human multiple myeloma cells (2007)
  • Author(s): Hideshima T, Catley L, Raje N, Chauhan D, Podar K, Mitsiades C, Tai YT, Vallet S, Kiziltepe T, Ocio E, Ikeda H, Okawa Y, Hideshima H, Munshi NC, Yasui H, Richardson PG, Anderson KC
  • Journal Title: Br J Haematol 138 Pages: 783-791
  • Peer Reviewed
• [Journal Article] MILN3897, a novel CCR1 inhibitor, impairs osteoclastogenesis and inhibits the interaction of multiple myeloma cells and osteoclasts (2007)
  • Author(s): Vallet S, Raje N, Ishitsuka K, Hideshima T, Podar K, Chhetri S, Pozzi S, Breitkreutz I, Kiziltepe T, Yasui H, Ocio EM, Shiraishi N, Jin J, Okawa Y, Ikeda H, Mukherjee S, Vaghela N, Cirstea D, Ladetto M, Boccadoro M, Anderson KC
  • Journal Title: Blood 110 Pages: 3744-3752
  • Peer Reviewed
• [Journal Article] 5-Azacytidine, a DNA methyltransferase inhibitor, induces ATR-mediated DNA double-strand break responses, apoptosis, and synergistic cytotoxicity with doxorubicin and bortezomib against multiple myeloma cells (2007)
  • Author(s): Kiziltepe T, Hideshima T, Catley L, Raje N, Yasui H, Shiraishi N, Okawa Y, Ikeda H, Vallet S, Pozzi S, Ishitsuka K, Ocio EM, Chauhan D, Anderson KC
  • Journal Title: Mol Cancer Ther 6 Pages: 1718-1727
  • Peer Reviewed
• [Journal Article] JS-K, a GST-activated nitric oxide generator, induces DNA double-strand breaks, activates DNA damage response pathways, and induces apoptosis in vitro and in vivo in human multiple myeloma cells (2007)
  • Author(s): Kiziltepe T, Hideshima T, Ishitsuka K, Ocio EM, Raje N, Catley L, Li CQ, Trudel LJ, Yasui H, Vallet S, Kutok JL, Chauhan D, Mitsiades CS, Saavedra JE, Wogan GN, Keefer LK, Shami PJ, Anderson KC
  • Journal Title: Blood 110 Pages: 709-718
  • Peer Reviewed
• [Journal Article] Targeting the beta-catenin/TCF transcriptional complex in the treatment of multiple myeloma (2007)
  • Author(s): Sukhdeo K, Mani M, Zhang Y, Dutta J, Yasui H, Roon e H, Tai YT, Mitsiades C, Anderson KC, Carrasco DR
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 7516-7521
  • Peer Reviewed
• [Journal Article] Up-regulation of c-Jun inhibits proliferation and induces apoptosis via caspase-triggered c-Abl cleavage in human multiple myeloma (2007)
  • Author(s): Podar K, Raab MS, Tonon G, Sattler M, Barila D, Zhang J, Tai YT, Yasui H, Raje N, DePinho RA, Hideshima T, Chauhan D, Anderson KC
  • Journal Title: Cancer Res 67 Pages: 1680-1688
  • Peer Reviewed
• [Journal Article] Nonsteroidal anti-inflammatory drugs and oxidative stress in cancer cells (2007)
  • Author(s): Adachi M, Sakamoto H, Kawamura R, Wang W, Imai K, Shinomura Y
  • Journal Title: Histol Histopathol 22 Pages: 437-442
  • Peer Reviewed
• [Journal Article] Biological pathways and in vivo antitumor activity induced by Atiprimod in myeloma. (2007)
  • Author(s): Neri P
  • Journal Title: Leukemia 21 Pages: 2519-2526
  • Peer Reviewed
• [Journal Article] MLN3897, a novel CCR1 inhibitor, impairs osteoclastogenesis and inhibits the interaction of multiple myeloma cells and osteoclasts. (2007)
  • Author(s): Vallet S
  • Journal Title: Blood 110 Pages: 3744-3752
  • Peer Reviewed
• [Journal Article] Targeting the beta-catenin/TCF transcriptional complex in the treatment of multiple myeloma. (2007)
  • Author(s): Sukhdeo K
  • Journal Title: Proc Natl Acad Sci U S A. 104 Pages: 7516-7521
  • Peer Reviewed
• [Presentation] XBP1の新しい機能 : 酸化ストレス応答への関与 (2008)
  • Author(s): 安達正晃、劉耀華、趙世光、Koong Albert, Rando Thomas, Dan Ruo, 今井浩三、篠村泰久
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] XBP1の新しい機能 : 酸化ストレス応答への関与 (2008)
  • Author(s): 安達 正晃
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Preventing oxidative stress : A new role for XBP1 and its candidate as a molecular target for cancer (2008)
  • Author(s): Adachi M, Liu Y, Zhao S, Hareyama M, Koong A, Imai K and Shinomura Y
  • Organizer: The 6^<th> International Symposium on cancer research and therapy
  • Place of Presentation: Tokyo
  • Year and Date: 2008-11-22
• [Presentation] (2008)
  • Author(s): 安達正晃、劉耀華、Koong Albert, 今井浩三、篠村泰久
  • Organizer: 第17回日本癌病態治療研究会
  • Place of Presentation: 京都
  • Year and Date: 2008-06-26
• [Presentation] IL-2シグナルと小胞体ストレスシグナル (2007)
  • Author(s): 安達正晃, 劉耀華、今井浩三、篠村泰久
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 内在性ホルモンを利用した新たな放射線感受性亢進療法の開発 (2007)
  • Author(s): 安達正晃、今井浩三、晴山雅人、篠村恭久
  • Organizer: 第16回日本癌病態治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-28
• [Book] 基質依存性増殖/アノイキス(Anoikis)改訂培養細胞実験ハンドブック (2008)
  • Author(s): 安達正晃
  • Publisher: 羊土社
• [Book] 遺伝子治療看護のための最新医学講座第2版24巻(腫瘍の臨床) (2008)
  • Author(s): 安達正晃
  • Publisher: 中山書店
• [Book] 改訂 培養細胞実験ハンドブック (2008)
  • Author(s): 安達 正晃
  • Total Pages: 330
  • Publisher: 羊土社