Clinical application of circulating nucleic acids as new biomarkers in patients with gastrointestinal cancers

• Project/Area Number: 19590568
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: ICHLKAWA Daisuke Kyoto Prefectural University of Medicine, 医学研究科, 助教 (20347446)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 胃癌 / 食道癌 / バイオマーカー / DNA断片 / 血漿

Research Abstract

癌患者の末梢血液中に、腫瘍細胞が崩壊することによつて放出されるDNAやRNA等の核酸が安定した状態で存在することが本研究で判明した。実際の胃癌や食道癌患者の末梢血液を用いた検討では、DNAの断片の濃度が健常者に比較して高濃度であることや、一部の患者において癌に特異的な異常分子の検出が可能であつた。DNAの繰り返し配列を用いた検討や、検索RNAを増やすことによって、感度や特異度が上がり、実地臨床に応用可能と思われた.

Research Products

• [Journal Article] Efficacy of prophylactic extended lymphadenectomy with gastrectomy for patlents wlth node- negatlve advanced gastric carcinoma (2008)
  • Author(s): Otsuji E, Ichikawa D, et.al.(他5名)
  • Journal Title: Hepato-gastroenterol 55 Pages: 755-759
  • Peer Reviewed
• [Journal Article] Long-term complete response of multiple hepatic metastases from carcinoma Of the papilla of Vater intrahepatic infusion of 5FU with low- dose cisplatin follOwing pancreaticoduodenectomy (2008)
  • Author(s): Komatsu s, Ichikawa D, et.al.(他6名)
  • Journal Title: Int J Clin Oncol 13 Pages: 567-570
  • Peer Reviewed
• [Journal Article] Efiicacy of prophylactic extended lymphadenectomy with gastrectomy for patients with node-negative advanced gastric cancer. (2008)
  • Author(s): 0tsuji E, Ichikawa D, et al
  • Journal Title: Hepatogastroenterology 55 Pages: 755-759
  • Peer Reviewed
• [Journal Article] Long-term complete response of multiple hepatic metastases from carcinoma of the papilla of Vater intrahepatic infusion of 5FU with low-dose cisplatin following pancreaticoduodenectomy (2008)
  • Author(s): Komatsu S, Ichikawa D, et al
  • Journal Title: Int J Clin Oncol 13 Pages: 567-570
  • Peer Reviewed
• [Journal Article] QuantificatiOn of plasma cell- free DNA in patlents with gastric cancer (2007)
  • Author(s): Sai S, Ichikawa D, et.al.(他8名)
  • Journal Title: Anticancer Res 27 Pages: 2747-2751
  • Peer Reviewed
• [Journal Article] Quantification of circulating plasma DNA fragments as tumor markers inpatients with esophageal cancer (2007)
  • Author(s): Tomita H,Ichikawa D, et.al.(他9名)
  • Journal Title: Anticancer Res 27 Pages: 2737-2741
  • Peer Reviewed
• [Journal Article] Clinical application of methylation specific-polymerase chain reaction inserum of patients with gastric cancer (2007)
  • Author(s): Ikoma H,Ichikawa D, et.al.(他8名)
  • Journal Title: Hepato-gastroenterol 54 Pages: 946-950
  • Peer Reviewed
• [Journal Article] Circtllating cell-free mRNA in plasma as a tumor marker for patlents wlth prlmary and recurrent gastrlc cancer (2007)
  • Author(s): Tani N, Ichikawa D, et.al.(他11名)
  • Journal Title: Anticancer Res 27 Pages: 1207-1212
  • Peer Reviewed
• [Journal Article] Circulating tumor cells and aberrant methylation as tumor markers in patients with esophageal cancer (2007)
  • Author(s): Ikoma D, Ichikawa D, et.al.(他8名)
  • Journal Title: Anticancer Res 27 Pages: 535-539
  • Peer Reviewed
• [Journal Article] 癌存在診断法としての食道癌・胃癌患者におけるCirculating DNA断片定量解析の検討 (2007)
  • Author(s): 富田晴久、市川大輔、他(他16名)
  • Journal Title: 癌と化学療法 34 Pages: 1908-1910
  • Peer Reviewed
• [Journal Article] Circulating tumor cells and aberrant methylation as tumor markers in patients with esophageal cancer (2007)
  • Author(s): Ikoma D, Ichikawa D, et. al.
  • Journal Title: Anticancer Research 27(1B) Pages: 535-539
  • Peer Reviewed
• [Journal Article] Circulating cell-free mRNA in plasma as a tumor marker for patients with primary and recurrent gastric cancer (2007)
  • Author(s): Tani N, Ichikawa D, et. al.
  • Journal Title: Anticancer Research 27(2) Pages: 1207-1212
  • Peer Reviewed
• [Journal Article] Quantification of circulating plasma DNA fragments as tumor markers in patients with esophageal cancer (2007)
  • Author(s): Tomita H, Ichikawa D, et. al.
  • Journal Title: Anticancer Research 27(4C) Pages: 2737-2741
  • Peer Reviewed
• [Journal Article] Quantification of plasma cell-free DNA in patients with gastric cancer. (2007)
  • Author(s): Sai S, Ichikawa D, et. al.
  • Journal Title: Anticancer Research 27(4C) Pages: 2747-2751
  • Peer Reviewed
• [Journal Article] Clinical application of methylation specific-polymerase chain reaction in serum of patients with gastric cancer (2007)
  • Author(s): Ikoma H, Ichikawa D, et. al.
  • Journal Title: Hepato-Gastroenterology 54 Pages: 946-950
  • Peer Reviewed
• [Presentation] 末梢血液中の核酸バイオマーカーを用いた癌存在診断の現状と展望 (2008)
  • Author(s): 市川大輔他
  • Organizer: 第46回日本癌治療学会総会
  • Place of Presentation: 名古屋
• [Presentation] 消化器癌における血中遊離癌細胞の臨床的意義 (2008)
  • Author(s): 市川大輔他
  • Organizer: 第108回日本外科学会総会
  • Place of Presentation: 長崎
• [Presentation] 胃癌患者末梢血液中の遊離核酸を用いた新たなバイオマーカー有用性の検討 (2007)
  • Author(s): 市川大輔他
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜