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Construction of efficient expression system of anti-tumor marker protein-monoclonal antibodies in transgenic plant.

Research Project

Project/Area Number 19590573
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionToho University

Principal Investigator

SATOH Hiroyuki  Toho University, 理学部, 准教授 (80187228)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords肝細胞癌 / αフェトプロテイン / PIVKA-II / 遺伝子組換え植物 / ペルオキシダーゼ / モノクローナル抗体 / 腫瘍マーカー検査 / AFP / plantibody / 腫瘍マーカー / ワサビ
Research Abstract

To reduce the production cost of monoclonal antibody for diagnosis, we tried to express gamma-globulin cDNAs of AFP and PIVKA-II in transgenic plant for the diagnosis of hepatocellular carcinoma. After we cloned the cDNAs, construction of cDNAs into scFv was made for its expression in transgenic-plant. As the other approach, we found some novel peroxidases possess higher activity from Japanese wsabi radish than that of horse radish peroxidase.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (5 results)

All 2008 2007

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] A Proteomic Profiling Approach to Reveal a Novel Role of BnD22 (Brassica napus drought 22 kD)/Water-Soluble Chlorophyll binding Protein in young leaves during Nitrogen Remobilization induced by Stressful conditions2008

    • Author(s)
      Desclos, et al.
    • Journal Title

      Plant Physiology 147

      Pages: 1830-1844

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural mechanism and photoprotective function of water-soluble chlorophyll-binding protein.2007

    • Author(s)
      Horigome, et. al.
    • Journal Title

      J. Biol. Chem., 282

      Pages: 6525-6531

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ニホンワサビペルオキシダーゼのcDNAクローニング2008

    • Author(s)
      小嶋拓也, 他
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 病原性放線菌Nocardia farcinicaにおけるアミノ配糖体リン酸化酵素遺伝子の同定2008

    • Author(s)
      藤井匠子, 他
    • Organizer
      2008年度日本放線菌学会大会
    • Place of Presentation
      山梨
    • Year and Date
      2008-07-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] ニホンワサビペルオキシダーゼのcDNAクローニング2008

    • Author(s)
      小嶋拓也, 佐藤浩之
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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