Construction of efficient expression system of anti-tumor marker protein-monoclonal antibodies in transgenic plant.
| Project/Area Number |
19590573
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Toho University |
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Principal Investigator |
SATOH Hiroyuki Toho University, 理学部, 准教授 (80187228)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肝細胞癌 / αフェトプロテイン / PIVKA-II / 遺伝子組換え植物 / ペルオキシダーゼ / モノクローナル抗体 / 腫瘍マーカー検査 / AFP / plantibody / 腫瘍マーカー / ワサビ |
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| Research Abstract |
To reduce the production cost of monoclonal antibody for diagnosis, we tried to express gamma-globulin cDNAs of AFP and PIVKA-II in transgenic plant for the diagnosis of hepatocellular carcinoma. After we cloned the cDNAs, construction of cDNAs into scFv was made for its expression in transgenic-plant. As the other approach, we found some novel peroxidases possess higher activity from Japanese wsabi radish than that of horse radish peroxidase.
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Report
(4 results)
Research Products
(5 results)
