Development of a novel DNA amplification methods by loop-mediated isothermal amplification in human herpesvirus infections.
| Project/Area Number |
19590577
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Fujita Health University (2008-2009)
Fujita Health University College (2007) |
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Principal Investigator |
IHIRA Masaru Fujita Health University, 医療科学部, 准教授 (10290165)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Hiroko 藤田保健衛生大学, 医学研究科, 研究員 (10387714)
ENOMOTO Yoshihiko 藤田保健衛生大学, 医学研究科, 研究員 (00387713)
YOSHIKAWA Tetsushi 藤田保健衛生大学, 医学部, 准教授 (80288472)
ASANO Yoshizo 藤田保健衛生大学, 医学部, 教授 (40131180)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 臨床検査医学 / HSV / LAMP / Real time PCR / 迅速診断 / Qprobe / HHV-6 / ウイルス分離 / IFA |
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| Research Abstract |
We constructed the rapid diagnostic methods using LAMP were developed for all 8 human herpesvirus infections. Viral DNA was detected in serum without DNA extraction by using LAMP. Furthermore, we developed type specific LAMP methods, which could discriminate between VZV vaccine strain and wild-type strain or between HHV-6 variant A and variant B. Qprobe is expected to become a new method for quantification of viral DNA by the LAMP method.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Elevated serum cytokine levels are associated with human herpesvirus 6 reactivation in hematopoietic stem cell transplantation recipients.2008
Author(s)
Fujita A, Ihira M, Suzuki R, Enomoto Y, Sugiyama H, Sugata K, Suga S, Asano Y, Yagasaki H, Kojima S, Matsumoto K, Kato K, Yoshikawa T.
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Journal Title
J. Infect. 57(3)
Pages: 241-248
Related Report
Peer Reviewed
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[Journal Article] Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method.2007
Author(s)
Ihira M, Akimoto S, Miyake F, Fujita A, Sugata K, Suga S, Ohashi M, Nishimura N, Ozaki T, Asano Y, Yoshikawa T.
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Journal Title
J Clin Virol. 39(1)
Pages: 22-26
Related Report
Peer Reviewed
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